House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters

Autores
Cid, Fabricio Damian; Fernandez, Noelia Celeste; Perez Chaca, Maria Veronica; Pardo Almudí, Rafael; Caviedes Vidal, Enrique Juan Raul; Chediack, Juan Gabriel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
House sparrows (Passer domesticus) have been proposed as a key ecological indicator of urban pollution. Remarkably, we lack knowledge about the physiological effects of lead on this bird species. Therefore, this study was aimed to evaluate the effect of Pb on several physiological parameters in house sparrows exposed to environmental Pb concentrations. In a first experiment, birds were exposed to Pb sub-lethal doses (from 1.3 to 14.0 µg of Pb/g animal/day) during 5 days, which resulted in a dose response increase of blood Pb levels and decrease of blood ALAD activity. However, at the higher doses tested (> 7 μg of Pb/g animal/day) the blood ALAD activity inhibition (~82%) remained constant. Hematocrit and hemoglobin were significantly reduced only at the highest-doses, and the stress indicator, heterophils to lymphocyte (H/L) ratio, did not show apparent changes. In a second experiment, house sparrows were exposed to Pb in drinking water (12.3 ppm) during either 15 or 30 days. Pb concentration used in this study was enough to produce blood lead levels equivalents to those found recently in house sparrows inhabiting urban areas, reduced blood ALAD activity and inversion of the H/L ratio. Decreasing blood ALAD activities were correlated with increasing blood Pb levels. In addition, Pb exposure produced modification in the levels of hepatic antioxidant enzymes, increased GST activity and decreased CAT activity, without lipid peroxidation. In conclusion, our results suggest that blood ALAD activity is a reliable and sensitive biomarker for environmental Pb exposure in house sparrows, additionally chronic exposure produce physiological stress (H/L inversion) and small changes in antioxidant enzyme activity. Finally, this specie could be considered a bioindicator for monitoring the urban Pb contamination.
Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina
Fil: Fernandez, Noelia Celeste. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina
Fil: Perez Chaca, Maria Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Pardo Almudí, Rafael. Universidad de Valladolid; España
Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. University of Wisconsin; Estados Unidos
Fil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina
Materia
ALAD
BIRDS
LEAD
OXIDATIVE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88978
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/88978
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parametersCid, Fabricio DamianFernandez, Noelia CelestePerez Chaca, Maria VeronicaPardo Almudí, RafaelCaviedes Vidal, Enrique Juan RaulChediack, Juan GabrielALADBIRDSLEADOXIDATIVE STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1House sparrows (Passer domesticus) have been proposed as a key ecological indicator of urban pollution. Remarkably, we lack knowledge about the physiological effects of lead on this bird species. Therefore, this study was aimed to evaluate the effect of Pb on several physiological parameters in house sparrows exposed to environmental Pb concentrations. In a first experiment, birds were exposed to Pb sub-lethal doses (from 1.3 to 14.0 µg of Pb/g animal/day) during 5 days, which resulted in a dose response increase of blood Pb levels and decrease of blood ALAD activity. However, at the higher doses tested (> 7 μg of Pb/g animal/day) the blood ALAD activity inhibition (~82%) remained constant. Hematocrit and hemoglobin were significantly reduced only at the highest-doses, and the stress indicator, heterophils to lymphocyte (H/L) ratio, did not show apparent changes. In a second experiment, house sparrows were exposed to Pb in drinking water (12.3 ppm) during either 15 or 30 days. Pb concentration used in this study was enough to produce blood lead levels equivalents to those found recently in house sparrows inhabiting urban areas, reduced blood ALAD activity and inversion of the H/L ratio. Decreasing blood ALAD activities were correlated with increasing blood Pb levels. In addition, Pb exposure produced modification in the levels of hepatic antioxidant enzymes, increased GST activity and decreased CAT activity, without lipid peroxidation. In conclusion, our results suggest that blood ALAD activity is a reliable and sensitive biomarker for environmental Pb exposure in house sparrows, additionally chronic exposure produce physiological stress (H/L inversion) and small changes in antioxidant enzyme activity. Finally, this specie could be considered a bioindicator for monitoring the urban Pb contamination.Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; ArgentinaFil: Fernandez, Noelia Celeste. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; ArgentinaFil: Perez Chaca, Maria Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Pardo Almudí, Rafael. Universidad de Valladolid; EspañaFil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. University of Wisconsin; Estados UnidosFil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; ArgentinaAcademic Press Inc Elsevier Science2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88978Cid, Fabricio Damian; Fernandez, Noelia Celeste; Perez Chaca, Maria Veronica; Pardo Almudí, Rafael; Caviedes Vidal, Enrique Juan Raul; et al.; House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 154; 6-2018; 154-1610147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S014765131830126Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2018.02.040info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:54Zoai:ri.conicet.gov.ar:11336/88978instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:54.527CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters
title House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters
spellingShingle House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters
Cid, Fabricio Damian
ALAD
BIRDS
LEAD
OXIDATIVE STRESS
title_short House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters
title_full House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters
title_fullStr House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters
title_full_unstemmed House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters
title_sort House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters
dc.creator.none.fl_str_mv Cid, Fabricio Damian
Fernandez, Noelia Celeste
Perez Chaca, Maria Veronica
Pardo Almudí, Rafael
Caviedes Vidal, Enrique Juan Raul
Chediack, Juan Gabriel
author Cid, Fabricio Damian
author_facet Cid, Fabricio Damian
Fernandez, Noelia Celeste
Perez Chaca, Maria Veronica
Pardo Almudí, Rafael
Caviedes Vidal, Enrique Juan Raul
Chediack, Juan Gabriel
author_role author
author2 Fernandez, Noelia Celeste
Perez Chaca, Maria Veronica
Pardo Almudí, Rafael
Caviedes Vidal, Enrique Juan Raul
Chediack, Juan Gabriel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALAD
BIRDS
LEAD
OXIDATIVE STRESS
topic ALAD
BIRDS
LEAD
OXIDATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv House sparrows (Passer domesticus) have been proposed as a key ecological indicator of urban pollution. Remarkably, we lack knowledge about the physiological effects of lead on this bird species. Therefore, this study was aimed to evaluate the effect of Pb on several physiological parameters in house sparrows exposed to environmental Pb concentrations. In a first experiment, birds were exposed to Pb sub-lethal doses (from 1.3 to 14.0 µg of Pb/g animal/day) during 5 days, which resulted in a dose response increase of blood Pb levels and decrease of blood ALAD activity. However, at the higher doses tested (> 7 μg of Pb/g animal/day) the blood ALAD activity inhibition (~82%) remained constant. Hematocrit and hemoglobin were significantly reduced only at the highest-doses, and the stress indicator, heterophils to lymphocyte (H/L) ratio, did not show apparent changes. In a second experiment, house sparrows were exposed to Pb in drinking water (12.3 ppm) during either 15 or 30 days. Pb concentration used in this study was enough to produce blood lead levels equivalents to those found recently in house sparrows inhabiting urban areas, reduced blood ALAD activity and inversion of the H/L ratio. Decreasing blood ALAD activities were correlated with increasing blood Pb levels. In addition, Pb exposure produced modification in the levels of hepatic antioxidant enzymes, increased GST activity and decreased CAT activity, without lipid peroxidation. In conclusion, our results suggest that blood ALAD activity is a reliable and sensitive biomarker for environmental Pb exposure in house sparrows, additionally chronic exposure produce physiological stress (H/L inversion) and small changes in antioxidant enzyme activity. Finally, this specie could be considered a bioindicator for monitoring the urban Pb contamination.
Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina
Fil: Fernandez, Noelia Celeste. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina
Fil: Perez Chaca, Maria Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Pardo Almudí, Rafael. Universidad de Valladolid; España
Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. University of Wisconsin; Estados Unidos
Fil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina
description House sparrows (Passer domesticus) have been proposed as a key ecological indicator of urban pollution. Remarkably, we lack knowledge about the physiological effects of lead on this bird species. Therefore, this study was aimed to evaluate the effect of Pb on several physiological parameters in house sparrows exposed to environmental Pb concentrations. In a first experiment, birds were exposed to Pb sub-lethal doses (from 1.3 to 14.0 µg of Pb/g animal/day) during 5 days, which resulted in a dose response increase of blood Pb levels and decrease of blood ALAD activity. However, at the higher doses tested (> 7 μg of Pb/g animal/day) the blood ALAD activity inhibition (~82%) remained constant. Hematocrit and hemoglobin were significantly reduced only at the highest-doses, and the stress indicator, heterophils to lymphocyte (H/L) ratio, did not show apparent changes. In a second experiment, house sparrows were exposed to Pb in drinking water (12.3 ppm) during either 15 or 30 days. Pb concentration used in this study was enough to produce blood lead levels equivalents to those found recently in house sparrows inhabiting urban areas, reduced blood ALAD activity and inversion of the H/L ratio. Decreasing blood ALAD activities were correlated with increasing blood Pb levels. In addition, Pb exposure produced modification in the levels of hepatic antioxidant enzymes, increased GST activity and decreased CAT activity, without lipid peroxidation. In conclusion, our results suggest that blood ALAD activity is a reliable and sensitive biomarker for environmental Pb exposure in house sparrows, additionally chronic exposure produce physiological stress (H/L inversion) and small changes in antioxidant enzyme activity. Finally, this specie could be considered a bioindicator for monitoring the urban Pb contamination.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88978
Cid, Fabricio Damian; Fernandez, Noelia Celeste; Perez Chaca, Maria Veronica; Pardo Almudí, Rafael; Caviedes Vidal, Enrique Juan Raul; et al.; House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 154; 6-2018; 154-161
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88978
identifier_str_mv Cid, Fabricio Damian; Fernandez, Noelia Celeste; Perez Chaca, Maria Veronica; Pardo Almudí, Rafael; Caviedes Vidal, Enrique Juan Raul; et al.; House sparrow biomarkers as lead pollution bioindicators: Evaluation of dose and exposition length on hematological and oxidative stress parameters; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 154; 6-2018; 154-161
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S014765131830126X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2018.02.040
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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