Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir
- Autores
- Moretti, Ana Florencia; Gamba, Raul Ricardo; do Céu Costa, María; de Antoni, Graciela Liliana; León Peláez, Angela Maria
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Kefir is a fermented beverage produced from kefir starter, by means of the milk fermentation with kefir grains. These grains are a protein and polysaccharide matrix that contains Lactic Acid Bacteria (LAB), Acetic Acid Bacteria (BAA) and yeasts. This study aimed to assess the protective activity of the lyophilization process on the activity of kefir grains by comparing their microbiological composition, sensory characteristics and antimicrobial effect on fermented products obtained either from lyophilized and fresh kefir grains. Acidification kinetics and microbial counts were performed on fermented milks obtained from fresh or freeze-dried grains. Inhibitory activity tests against Escherichia coli and Salmonella enterica serovar Enteritidis were carried out with different dilutions of cell free supernatants from fermented milks. On the other hand, similarity and acceptability tests were performed to compare the sensory characteristics of fermented milks obtained from fresh and freeze-dried grains. The microbiological composition and acidification capability of the freeze-dried grains were stable until 6 months of storage at 4°C. Moreover, the minimum inhibitory concentration of fresh and freeze-dried grains was similar (20% v/v) for both pathogens. Additionally, the sensory panel did not detect differences between milk fermented with fresh or freeze-dried kefir grains; the kefir beverage obtained from the freeze-dried kefir grains fermentation showed a wide sensory acceptance among the population surveyed. We considered that lyophilization followed by the storage conditions applied were suitable for the survival of the kefir micro biota, the sensory characteristics and the antimicrobial capability of the fermented beverage after an adequate reactivation process of the grains. In conclusion, the reactivation process risk management is an important stage to ensure a suitable carriage and therefore maximize the kefir grains for large scale use.
Fil: Moretti, Ana Florencia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Gamba, Raul Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: do Céu Costa, María. Universidade Lusófona. Escola de Ciências e Tecnologias da Saúde; Portugal
Fil: de Antoni, Graciela Liliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: León Peláez, Angela Maria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina - Materia
-
LYOPHILIZATION
KEFIR
ANTIMICROBIAL ACTIVITY
ESCHERICHIA COLI
SALMONELLA ENTERICA SEROVAR ENTERITIDIS
SENSORY EVALUATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/118624
Ver los metadatos del registro completo
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Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of KefirMoretti, Ana FlorenciaGamba, Raul Ricardodo Céu Costa, Maríade Antoni, Graciela LilianaLeón Peláez, Angela MariaLYOPHILIZATIONKEFIRANTIMICROBIAL ACTIVITYESCHERICHIA COLISALMONELLA ENTERICA SEROVAR ENTERITIDISSENSORY EVALUATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Kefir is a fermented beverage produced from kefir starter, by means of the milk fermentation with kefir grains. These grains are a protein and polysaccharide matrix that contains Lactic Acid Bacteria (LAB), Acetic Acid Bacteria (BAA) and yeasts. This study aimed to assess the protective activity of the lyophilization process on the activity of kefir grains by comparing their microbiological composition, sensory characteristics and antimicrobial effect on fermented products obtained either from lyophilized and fresh kefir grains. Acidification kinetics and microbial counts were performed on fermented milks obtained from fresh or freeze-dried grains. Inhibitory activity tests against Escherichia coli and Salmonella enterica serovar Enteritidis were carried out with different dilutions of cell free supernatants from fermented milks. On the other hand, similarity and acceptability tests were performed to compare the sensory characteristics of fermented milks obtained from fresh and freeze-dried grains. The microbiological composition and acidification capability of the freeze-dried grains were stable until 6 months of storage at 4°C. Moreover, the minimum inhibitory concentration of fresh and freeze-dried grains was similar (20% v/v) for both pathogens. Additionally, the sensory panel did not detect differences between milk fermented with fresh or freeze-dried kefir grains; the kefir beverage obtained from the freeze-dried kefir grains fermentation showed a wide sensory acceptance among the population surveyed. We considered that lyophilization followed by the storage conditions applied were suitable for the survival of the kefir micro biota, the sensory characteristics and the antimicrobial capability of the fermented beverage after an adequate reactivation process of the grains. In conclusion, the reactivation process risk management is an important stage to ensure a suitable carriage and therefore maximize the kefir grains for large scale use.Fil: Moretti, Ana Florencia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Gamba, Raul Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: do Céu Costa, María. Universidade Lusófona. Escola de Ciências e Tecnologias da Saúde; PortugalFil: de Antoni, Graciela Liliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: León Peláez, Angela Maria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; ArgentinaMadridge Publishers2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118624Moretti, Ana Florencia; Gamba, Raul Ricardo; do Céu Costa, María; de Antoni, Graciela Liliana; León Peláez, Angela Maria; Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir; Madridge Publishers; International Journal of Biochemistry and Pharmacology; 1; 1; 7-2019; 1-72689-7695CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.18689/ijbp-1000102info:eu-repo/semantics/altIdentifier/url/https://tinyurl.com/yymuqwnzinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:32Zoai:ri.conicet.gov.ar:11336/118624instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:32.414CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir |
title |
Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir |
spellingShingle |
Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir Moretti, Ana Florencia LYOPHILIZATION KEFIR ANTIMICROBIAL ACTIVITY ESCHERICHIA COLI SALMONELLA ENTERICA SEROVAR ENTERITIDIS SENSORY EVALUATION |
title_short |
Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir |
title_full |
Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir |
title_fullStr |
Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir |
title_full_unstemmed |
Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir |
title_sort |
Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir |
dc.creator.none.fl_str_mv |
Moretti, Ana Florencia Gamba, Raul Ricardo do Céu Costa, María de Antoni, Graciela Liliana León Peláez, Angela Maria |
author |
Moretti, Ana Florencia |
author_facet |
Moretti, Ana Florencia Gamba, Raul Ricardo do Céu Costa, María de Antoni, Graciela Liliana León Peláez, Angela Maria |
author_role |
author |
author2 |
Gamba, Raul Ricardo do Céu Costa, María de Antoni, Graciela Liliana León Peláez, Angela Maria |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
LYOPHILIZATION KEFIR ANTIMICROBIAL ACTIVITY ESCHERICHIA COLI SALMONELLA ENTERICA SEROVAR ENTERITIDIS SENSORY EVALUATION |
topic |
LYOPHILIZATION KEFIR ANTIMICROBIAL ACTIVITY ESCHERICHIA COLI SALMONELLA ENTERICA SEROVAR ENTERITIDIS SENSORY EVALUATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Kefir is a fermented beverage produced from kefir starter, by means of the milk fermentation with kefir grains. These grains are a protein and polysaccharide matrix that contains Lactic Acid Bacteria (LAB), Acetic Acid Bacteria (BAA) and yeasts. This study aimed to assess the protective activity of the lyophilization process on the activity of kefir grains by comparing their microbiological composition, sensory characteristics and antimicrobial effect on fermented products obtained either from lyophilized and fresh kefir grains. Acidification kinetics and microbial counts were performed on fermented milks obtained from fresh or freeze-dried grains. Inhibitory activity tests against Escherichia coli and Salmonella enterica serovar Enteritidis were carried out with different dilutions of cell free supernatants from fermented milks. On the other hand, similarity and acceptability tests were performed to compare the sensory characteristics of fermented milks obtained from fresh and freeze-dried grains. The microbiological composition and acidification capability of the freeze-dried grains were stable until 6 months of storage at 4°C. Moreover, the minimum inhibitory concentration of fresh and freeze-dried grains was similar (20% v/v) for both pathogens. Additionally, the sensory panel did not detect differences between milk fermented with fresh or freeze-dried kefir grains; the kefir beverage obtained from the freeze-dried kefir grains fermentation showed a wide sensory acceptance among the population surveyed. We considered that lyophilization followed by the storage conditions applied were suitable for the survival of the kefir micro biota, the sensory characteristics and the antimicrobial capability of the fermented beverage after an adequate reactivation process of the grains. In conclusion, the reactivation process risk management is an important stage to ensure a suitable carriage and therefore maximize the kefir grains for large scale use. Fil: Moretti, Ana Florencia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Gamba, Raul Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: do Céu Costa, María. Universidade Lusófona. Escola de Ciências e Tecnologias da Saúde; Portugal Fil: de Antoni, Graciela Liliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: León Peláez, Angela Maria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina |
description |
Kefir is a fermented beverage produced from kefir starter, by means of the milk fermentation with kefir grains. These grains are a protein and polysaccharide matrix that contains Lactic Acid Bacteria (LAB), Acetic Acid Bacteria (BAA) and yeasts. This study aimed to assess the protective activity of the lyophilization process on the activity of kefir grains by comparing their microbiological composition, sensory characteristics and antimicrobial effect on fermented products obtained either from lyophilized and fresh kefir grains. Acidification kinetics and microbial counts were performed on fermented milks obtained from fresh or freeze-dried grains. Inhibitory activity tests against Escherichia coli and Salmonella enterica serovar Enteritidis were carried out with different dilutions of cell free supernatants from fermented milks. On the other hand, similarity and acceptability tests were performed to compare the sensory characteristics of fermented milks obtained from fresh and freeze-dried grains. The microbiological composition and acidification capability of the freeze-dried grains were stable until 6 months of storage at 4°C. Moreover, the minimum inhibitory concentration of fresh and freeze-dried grains was similar (20% v/v) for both pathogens. Additionally, the sensory panel did not detect differences between milk fermented with fresh or freeze-dried kefir grains; the kefir beverage obtained from the freeze-dried kefir grains fermentation showed a wide sensory acceptance among the population surveyed. We considered that lyophilization followed by the storage conditions applied were suitable for the survival of the kefir micro biota, the sensory characteristics and the antimicrobial capability of the fermented beverage after an adequate reactivation process of the grains. In conclusion, the reactivation process risk management is an important stage to ensure a suitable carriage and therefore maximize the kefir grains for large scale use. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/118624 Moretti, Ana Florencia; Gamba, Raul Ricardo; do Céu Costa, María; de Antoni, Graciela Liliana; León Peláez, Angela Maria; Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir; Madridge Publishers; International Journal of Biochemistry and Pharmacology; 1; 1; 7-2019; 1-7 2689-7695 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/118624 |
identifier_str_mv |
Moretti, Ana Florencia; Gamba, Raul Ricardo; do Céu Costa, María; de Antoni, Graciela Liliana; León Peláez, Angela Maria; Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir; Madridge Publishers; International Journal of Biochemistry and Pharmacology; 1; 1; 7-2019; 1-7 2689-7695 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.18689/ijbp-1000102 info:eu-repo/semantics/altIdentifier/url/https://tinyurl.com/yymuqwnz |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Madridge Publishers |
publisher.none.fl_str_mv |
Madridge Publishers |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614167499112448 |
score |
13.070432 |