Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition

Autores
Raffo Iraolagoitia, Ximena Lucía; Martini, María Florencia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work is to determine the binding of ionic calcium (Ca2+) to lipid membranes in which the availability of the phosphate groups to the aqueous phase is modified by the degree of saturation of the lipids and the inclusion of cholesterol. The shifts in the phosphate bands observed in the Fourier transform infrared spectroscopy (FTIR) spectra are direct evidence of the interaction of Ca2+ with phosphate groups. The binding analysis was done by determining the changes in the zeta potential of liposomes suspended in buffer at controlled temperature. The changes produced by the ion on the zeta potential of dioleoylphosphatidylcholine (DOPC); dipalmitoylphosphatidylcholine (DPPC); distearoylphosphatidylcholine (DSPC); dimyristoylphosphatidylethanolamine (DMPE) and their mixers with cholesterol were measured, showing a Langmuir isotherm behavior in all the lipid composition assayed. The results show that the interaction of Ca2+ to lipid membranes depends on the exposure and the density of phosphate groups at the membrane interphase.
Fil: Raffo Iraolagoitia, Ximena Lucía. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martini, María Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Lipid Membrane
Phosphate Groups
Ionic Calcium
Cholesterol
Lipid-Calcium Interaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16285
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16285
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ca2+ adsorption to lipid membranes and the effect of cholesterol in their compositionRaffo Iraolagoitia, Ximena LucíaMartini, María FlorenciaLipid MembranePhosphate GroupsIonic CalciumCholesterolLipid-Calcium Interactionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work is to determine the binding of ionic calcium (Ca2+) to lipid membranes in which the availability of the phosphate groups to the aqueous phase is modified by the degree of saturation of the lipids and the inclusion of cholesterol. The shifts in the phosphate bands observed in the Fourier transform infrared spectroscopy (FTIR) spectra are direct evidence of the interaction of Ca2+ with phosphate groups. The binding analysis was done by determining the changes in the zeta potential of liposomes suspended in buffer at controlled temperature. The changes produced by the ion on the zeta potential of dioleoylphosphatidylcholine (DOPC); dipalmitoylphosphatidylcholine (DPPC); distearoylphosphatidylcholine (DSPC); dimyristoylphosphatidylethanolamine (DMPE) and their mixers with cholesterol were measured, showing a Langmuir isotherm behavior in all the lipid composition assayed. The results show that the interaction of Ca2+ to lipid membranes depends on the exposure and the density of phosphate groups at the membrane interphase.Fil: Raffo Iraolagoitia, Ximena Lucía. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martini, María Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16285Raffo Iraolagoitia, Ximena Lucía; Martini, María Florencia; Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 76; 1; 3-2010; 215-2200927-7765enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776509005311info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2009.10.037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:26Zoai:ri.conicet.gov.ar:11336/16285instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:26.351CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition
title Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition
spellingShingle Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition
Raffo Iraolagoitia, Ximena Lucía
Lipid Membrane
Phosphate Groups
Ionic Calcium
Cholesterol
Lipid-Calcium Interaction
title_short Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition
title_full Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition
title_fullStr Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition
title_full_unstemmed Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition
title_sort Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition
dc.creator.none.fl_str_mv Raffo Iraolagoitia, Ximena Lucía
Martini, María Florencia
author Raffo Iraolagoitia, Ximena Lucía
author_facet Raffo Iraolagoitia, Ximena Lucía
Martini, María Florencia
author_role author
author2 Martini, María Florencia
author2_role author
dc.subject.none.fl_str_mv Lipid Membrane
Phosphate Groups
Ionic Calcium
Cholesterol
Lipid-Calcium Interaction
topic Lipid Membrane
Phosphate Groups
Ionic Calcium
Cholesterol
Lipid-Calcium Interaction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work is to determine the binding of ionic calcium (Ca2+) to lipid membranes in which the availability of the phosphate groups to the aqueous phase is modified by the degree of saturation of the lipids and the inclusion of cholesterol. The shifts in the phosphate bands observed in the Fourier transform infrared spectroscopy (FTIR) spectra are direct evidence of the interaction of Ca2+ with phosphate groups. The binding analysis was done by determining the changes in the zeta potential of liposomes suspended in buffer at controlled temperature. The changes produced by the ion on the zeta potential of dioleoylphosphatidylcholine (DOPC); dipalmitoylphosphatidylcholine (DPPC); distearoylphosphatidylcholine (DSPC); dimyristoylphosphatidylethanolamine (DMPE) and their mixers with cholesterol were measured, showing a Langmuir isotherm behavior in all the lipid composition assayed. The results show that the interaction of Ca2+ to lipid membranes depends on the exposure and the density of phosphate groups at the membrane interphase.
Fil: Raffo Iraolagoitia, Ximena Lucía. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martini, María Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this work is to determine the binding of ionic calcium (Ca2+) to lipid membranes in which the availability of the phosphate groups to the aqueous phase is modified by the degree of saturation of the lipids and the inclusion of cholesterol. The shifts in the phosphate bands observed in the Fourier transform infrared spectroscopy (FTIR) spectra are direct evidence of the interaction of Ca2+ with phosphate groups. The binding analysis was done by determining the changes in the zeta potential of liposomes suspended in buffer at controlled temperature. The changes produced by the ion on the zeta potential of dioleoylphosphatidylcholine (DOPC); dipalmitoylphosphatidylcholine (DPPC); distearoylphosphatidylcholine (DSPC); dimyristoylphosphatidylethanolamine (DMPE) and their mixers with cholesterol were measured, showing a Langmuir isotherm behavior in all the lipid composition assayed. The results show that the interaction of Ca2+ to lipid membranes depends on the exposure and the density of phosphate groups at the membrane interphase.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16285
Raffo Iraolagoitia, Ximena Lucía; Martini, María Florencia; Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 76; 1; 3-2010; 215-220
0927-7765
url http://hdl.handle.net/11336/16285
identifier_str_mv Raffo Iraolagoitia, Ximena Lucía; Martini, María Florencia; Ca2+ adsorption to lipid membranes and the effect of cholesterol in their composition; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 76; 1; 3-2010; 215-220
0927-7765
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776509005311
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2009.10.037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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