Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
- Autores
- Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network.
Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina - Materia
-
BLACK LIPID MEMBRANES
CHOLESTEROL
DETRENDED FLUCTUATION ANALYSIS
IONIC CURRENT
LIPID PORES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1517
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Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayersCorvalán, Natalia AndreaKembro, Jackelyn MelissaClop, Pedro DiegoPerillo, Maria AngelicaBLACK LIPID MEMBRANESCHOLESTEROLDETRENDED FLUCTUATION ANALYSISIONIC CURRENTLIPID POREShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network.Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaElsevier Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1517Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica; Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1828; 8; 8-2013; 1754-17640005-2736enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2013.03.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:35Zoai:ri.conicet.gov.ar:11336/1517instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:35.744CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers |
title |
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers |
spellingShingle |
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers Corvalán, Natalia Andrea BLACK LIPID MEMBRANES CHOLESTEROL DETRENDED FLUCTUATION ANALYSIS IONIC CURRENT LIPID PORES |
title_short |
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers |
title_full |
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers |
title_fullStr |
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers |
title_full_unstemmed |
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers |
title_sort |
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers |
dc.creator.none.fl_str_mv |
Corvalán, Natalia Andrea Kembro, Jackelyn Melissa Clop, Pedro Diego Perillo, Maria Angelica |
author |
Corvalán, Natalia Andrea |
author_facet |
Corvalán, Natalia Andrea Kembro, Jackelyn Melissa Clop, Pedro Diego Perillo, Maria Angelica |
author_role |
author |
author2 |
Kembro, Jackelyn Melissa Clop, Pedro Diego Perillo, Maria Angelica |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
BLACK LIPID MEMBRANES CHOLESTEROL DETRENDED FLUCTUATION ANALYSIS IONIC CURRENT LIPID PORES |
topic |
BLACK LIPID MEMBRANES CHOLESTEROL DETRENDED FLUCTUATION ANALYSIS IONIC CURRENT LIPID PORES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network. Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina |
description |
The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/1517 Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica; Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1828; 8; 8-2013; 1754-1764 0005-2736 |
url |
http://hdl.handle.net/11336/1517 |
identifier_str_mv |
Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica; Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1828; 8; 8-2013; 1754-1764 0005-2736 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2013.03.019 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |