Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers

Autores
Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network.
Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
BLACK LIPID MEMBRANES
CHOLESTEROL
DETRENDED FLUCTUATION ANALYSIS
IONIC CURRENT
LIPID PORES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1517
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1517
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayersCorvalán, Natalia AndreaKembro, Jackelyn MelissaClop, Pedro DiegoPerillo, Maria AngelicaBLACK LIPID MEMBRANESCHOLESTEROLDETRENDED FLUCTUATION ANALYSISIONIC CURRENTLIPID POREShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network.Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaElsevier Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1517Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica; Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1828; 8; 8-2013; 1754-17640005-2736enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2013.03.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:40:32Zoai:ri.conicet.gov.ar:11336/1517instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:40:33.272CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
title Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
spellingShingle Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
Corvalán, Natalia Andrea
BLACK LIPID MEMBRANES
CHOLESTEROL
DETRENDED FLUCTUATION ANALYSIS
IONIC CURRENT
LIPID PORES
title_short Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
title_full Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
title_fullStr Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
title_full_unstemmed Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
title_sort Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
dc.creator.none.fl_str_mv Corvalán, Natalia Andrea
Kembro, Jackelyn Melissa
Clop, Pedro Diego
Perillo, Maria Angelica
author Corvalán, Natalia Andrea
author_facet Corvalán, Natalia Andrea
Kembro, Jackelyn Melissa
Clop, Pedro Diego
Perillo, Maria Angelica
author_role author
author2 Kembro, Jackelyn Melissa
Clop, Pedro Diego
Perillo, Maria Angelica
author2_role author
author
author
dc.subject.none.fl_str_mv BLACK LIPID MEMBRANES
CHOLESTEROL
DETRENDED FLUCTUATION ANALYSIS
IONIC CURRENT
LIPID PORES
topic BLACK LIPID MEMBRANES
CHOLESTEROL
DETRENDED FLUCTUATION ANALYSIS
IONIC CURRENT
LIPID PORES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network.
Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1517
Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica; Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1828; 8; 8-2013; 1754-1764
0005-2736
url http://hdl.handle.net/11336/1517
identifier_str_mv Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica; Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1828; 8; 8-2013; 1754-1764
0005-2736
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2013.03.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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