Influenza A virus negative strand RNA is translated for CD8+ T cell immunosurveillance

• Autores: Hickman, Heather D.; Mays, Jacqueline W.; Gibbs, James; Kosik, Ivan; Magadan, Javier Guillermo; Takeda, Kazuyo; Das, Suman; Reynoso, Glennys V.; Ngudiankama, Barbara F.; Wei, Jiajie; Shannon, John P.; McManus, Daniel; Yewdell, Jonathan W.
• Año de publicación: 2018
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: INFLUENZA A; NEG8; IMMUNOSURVEILLANCE
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Probing the limits of CD8+ T cell immunosurveillance, we inserted the SIINFEKL peptide into influenza A virus (IAV)-negative strand gene segments. Although IAV genomic RNA is considered noncoding, there is a conserved, relatively long open reading frame present in segment 8, encoding a potential protein termed NEG8. The biosynthesis of NEG8 from IAV has yet to be demonstrated. Although we failed to detect NEG8 protein expression in IAV-infected mouse cells, cell surface Kb-SIINFEKL complexes are generated when SIINFEKL is genetically appended to the predicted C terminus of NEG8, as shown by activation of OT-I T cells in vitro and in vivo. Moreover, recombinant IAV encoding of SIINFEKL embedded in the negative strand of the neuraminidase-stalk coding sequence also activates OT-I T cells in mice. Together, our findings demonstrate both the translation of sequences on the negative strand of a single-stranded RNAvirus and its relevance in antiviral immunosurveillance.
Fil: Hickman, Heather D.. National Institute Of Allergy And Infectious Diseases;
Fil: Mays, Jacqueline W.. National Institute Of Allergy And Infectious Diseases;
Fil: Gibbs, James. National Institute Of Allergy And Infectious Diseases;
Fil: Kosik, Ivan. National Institute Of Allergy And Infectious Diseases;
Fil: Magadan, Javier Guillermo. National Institute Of Allergy And Infectious Diseases; . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo; Argentina
Fil: Takeda, Kazuyo. Food And Drug Administration;
Fil: Das, Suman. National Institute Of Allergy And Infectious Diseases;
Fil: Reynoso, Glennys V.. National Institute Of Allergy And Infectious Diseases;
Fil: Ngudiankama, Barbara F.. National Institute Of Allergy And Infectious Diseases;
Fil: Wei, Jiajie. National Institute Of Allergy And Infectious Diseases;
Fil: Shannon, John P.. National Institute Of Allergy And Infectious Diseases;
Fil: McManus, Daniel. National Institute Of Allergy And Infectious Diseases;
Fil: Yewdell, Jonathan W.. National Institute Of Allergy And Infectious Diseases;