External guide sequence technology: a path to development of novel antimicrobial therapeutics

Autores
Davies Sala, Carol Giselle; Soler Bistue, Alfonso J. C.; Bonomo, Robert A.; Zorreguieta, Angeles; Tolmasky, Marcelo E.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
RNase P is a ribozyme originally identified for its role in maturation of tRNAs by cleavage of precursor tRNAs (pre-tRNAs) at the 5'-end termini. RNase P is a ribonucleoprotein consisting of a catalytic RNA molecule and, depending on the organism, one or more cofactor proteins. The site of cleavage of a pre-tRNA is identified by its tertiary structure; and any RNA molecule can be cleaved by RNase P as long as the RNA forms a duplex that resembles the regional structure in the pre-tRNA. When the antisense sequence that forms the duplex with the strand that is subsequently cleaved by RNase P is in a separate molecule, it is called an external guide sequence (EGS). These fundamental observations are the basis for EGS technology, which consists of inhibiting gene expression by utilizing an EGS that elicits RNase P-mediated cleavage of a target mRNA molecule. EGS technology has been used to inhibit expression of a wide variety of genes, and may help development of novel treatments of diseases, including multidrug-resistant bacterial and viral infections.
Fil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. California State University Fullerton; Estados Unidos. Fundación Instituto Leloir; Argentina
Fil: Soler Bistue, Alfonso Jc. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. California State University Fullerton; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Materia
Antimicrobianos
Nuevas Terapias
Tecnología Antisentido
Ribozima P
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9863
Acceder
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network_name_str CONICET Digital (CONICET)
spelling External guide sequence technology: a path to development of novel antimicrobial therapeuticsDavies Sala, Carol GiselleSoler Bistue, Alfonso J. C.Bonomo, Robert A.Zorreguieta, AngelesTolmasky, Marcelo E.AntimicrobianosNuevas TerapiasTecnología AntisentidoRibozima Phttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1RNase P is a ribozyme originally identified for its role in maturation of tRNAs by cleavage of precursor tRNAs (pre-tRNAs) at the 5'-end termini. RNase P is a ribonucleoprotein consisting of a catalytic RNA molecule and, depending on the organism, one or more cofactor proteins. The site of cleavage of a pre-tRNA is identified by its tertiary structure; and any RNA molecule can be cleaved by RNase P as long as the RNA forms a duplex that resembles the regional structure in the pre-tRNA. When the antisense sequence that forms the duplex with the strand that is subsequently cleaved by RNase P is in a separate molecule, it is called an external guide sequence (EGS). These fundamental observations are the basis for EGS technology, which consists of inhibiting gene expression by utilizing an EGS that elicits RNase P-mediated cleavage of a target mRNA molecule. EGS technology has been used to inhibit expression of a wide variety of genes, and may help development of novel treatments of diseases, including multidrug-resistant bacterial and viral infections.Fil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. California State University Fullerton; Estados Unidos. Fundación Instituto Leloir; ArgentinaFil: Soler Bistue, Alfonso Jc. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. California State University Fullerton; Estados UnidosFil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados UnidosFil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Tolmasky, Marcelo E.. California State University; Estados UnidosNew York Academy of Sciences2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9863Davies Sala, Carol Giselle; Soler Bistue, Alfonso J. C.; Bonomo, Robert A.; Zorreguieta, Angeles; Tolmasky, Marcelo E.; External guide sequence technology: a path to development of novel antimicrobial therapeutics; New York Academy of Sciences; Annals Of The New York Academy Of Sciences.; 1354; 9-2015; 98-1100077-8923enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nyas.12755/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/nyas.12755info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600001/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:31Zoai:ri.conicet.gov.ar:11336/9863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:31.459CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv External guide sequence technology: a path to development of novel antimicrobial therapeutics
title External guide sequence technology: a path to development of novel antimicrobial therapeutics
spellingShingle External guide sequence technology: a path to development of novel antimicrobial therapeutics
Davies Sala, Carol Giselle
Antimicrobianos
Nuevas Terapias
Tecnología Antisentido
Ribozima P
title_short External guide sequence technology: a path to development of novel antimicrobial therapeutics
title_full External guide sequence technology: a path to development of novel antimicrobial therapeutics
title_fullStr External guide sequence technology: a path to development of novel antimicrobial therapeutics
title_full_unstemmed External guide sequence technology: a path to development of novel antimicrobial therapeutics
title_sort External guide sequence technology: a path to development of novel antimicrobial therapeutics
dc.creator.none.fl_str_mv Davies Sala, Carol Giselle
Soler Bistue, Alfonso J. C.
Bonomo, Robert A.
Zorreguieta, Angeles
Tolmasky, Marcelo E.
author Davies Sala, Carol Giselle
author_facet Davies Sala, Carol Giselle
Soler Bistue, Alfonso J. C.
Bonomo, Robert A.
Zorreguieta, Angeles
Tolmasky, Marcelo E.
author_role author
author2 Soler Bistue, Alfonso J. C.
Bonomo, Robert A.
Zorreguieta, Angeles
Tolmasky, Marcelo E.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antimicrobianos
Nuevas Terapias
Tecnología Antisentido
Ribozima P
topic Antimicrobianos
Nuevas Terapias
Tecnología Antisentido
Ribozima P
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv RNase P is a ribozyme originally identified for its role in maturation of tRNAs by cleavage of precursor tRNAs (pre-tRNAs) at the 5'-end termini. RNase P is a ribonucleoprotein consisting of a catalytic RNA molecule and, depending on the organism, one or more cofactor proteins. The site of cleavage of a pre-tRNA is identified by its tertiary structure; and any RNA molecule can be cleaved by RNase P as long as the RNA forms a duplex that resembles the regional structure in the pre-tRNA. When the antisense sequence that forms the duplex with the strand that is subsequently cleaved by RNase P is in a separate molecule, it is called an external guide sequence (EGS). These fundamental observations are the basis for EGS technology, which consists of inhibiting gene expression by utilizing an EGS that elicits RNase P-mediated cleavage of a target mRNA molecule. EGS technology has been used to inhibit expression of a wide variety of genes, and may help development of novel treatments of diseases, including multidrug-resistant bacterial and viral infections.
Fil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. California State University Fullerton; Estados Unidos. Fundación Instituto Leloir; Argentina
Fil: Soler Bistue, Alfonso Jc. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. California State University Fullerton; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
description RNase P is a ribozyme originally identified for its role in maturation of tRNAs by cleavage of precursor tRNAs (pre-tRNAs) at the 5'-end termini. RNase P is a ribonucleoprotein consisting of a catalytic RNA molecule and, depending on the organism, one or more cofactor proteins. The site of cleavage of a pre-tRNA is identified by its tertiary structure; and any RNA molecule can be cleaved by RNase P as long as the RNA forms a duplex that resembles the regional structure in the pre-tRNA. When the antisense sequence that forms the duplex with the strand that is subsequently cleaved by RNase P is in a separate molecule, it is called an external guide sequence (EGS). These fundamental observations are the basis for EGS technology, which consists of inhibiting gene expression by utilizing an EGS that elicits RNase P-mediated cleavage of a target mRNA molecule. EGS technology has been used to inhibit expression of a wide variety of genes, and may help development of novel treatments of diseases, including multidrug-resistant bacterial and viral infections.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9863
Davies Sala, Carol Giselle; Soler Bistue, Alfonso J. C.; Bonomo, Robert A.; Zorreguieta, Angeles; Tolmasky, Marcelo E.; External guide sequence technology: a path to development of novel antimicrobial therapeutics; New York Academy of Sciences; Annals Of The New York Academy Of Sciences.; 1354; 9-2015; 98-110
0077-8923
url http://hdl.handle.net/11336/9863
identifier_str_mv Davies Sala, Carol Giselle; Soler Bistue, Alfonso J. C.; Bonomo, Robert A.; Zorreguieta, Angeles; Tolmasky, Marcelo E.; External guide sequence technology: a path to development of novel antimicrobial therapeutics; New York Academy of Sciences; Annals Of The New York Academy Of Sciences.; 1354; 9-2015; 98-110
0077-8923
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nyas.12755/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/nyas.12755
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600001/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv New York Academy of Sciences
publisher.none.fl_str_mv New York Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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