Pancreatic enzyme activity in passer domesticus exposed to corticosterone

Autores
Padrones, Marcelo Nicolás; Cid, Fabricio Damian; Chediack, Juan Gabriel
Año de publicación
2021
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In animals, it is well documented that stress produces metabolic alterations and an allostatic response (activation of the hypothalamicpituitary-adrenal axis and increase of corticosterone levels in blood) to maintain homeostasis in organisms. The objective of this work was to study the long-term corticosterone (CORT) effects on the digestive function in Passer domesticus. We focus especially on modifications in the activity of pancreatic enzymes, trypsin and chymotrypsin. To achieve our goal, 32 sparrows were exposed to different concentrations of CORT in drinking water (0, 20, 40 and 80 mg/l) for 72 hours emulating a long-term stress situation. After treatment, body weight was measured, blood extraction and subsequent removal of digestive organs (intestine, liver and pancreas) were performed at the same time (8:00 am), to avoid disturb by circadian/daily variation. The Heterophil/Lymphocyte (H/L) ratio was used as an indicator of stress in birds. The mass of pancreas was measured and homogenized. The enzymatic activity of trypsin and chymotrypsin were determined. Previously, chymotrypsinogen was activated by enterokinase for 60 min to measure chymotrypsin activity. We analyzed the mass-specific enzyme activities (μmol min−1 g wet tissue−1) using one-way ANOVA with Tukey post-hoc test (p < 0.05). We found a proportional increased inthe H/L index in concordance with CORT treatments. It was not found differences in the mass of pancreas and activity of enzymes (p > 0.05). Studies in chickens show a variation in digestive enzymes by corticosterone. However, our case in wild birds, we didn´t found effect of CORT on pancreatic enzyme activities. Previously, we found similar results with intestinal enzymes (carbohydrases and proteases). So, future research will be in direction to comparative studies in poultry and wild birds, under the same experimental design, to dilucidated mechanism involved in enzyme modulation by CORT. Supported by CyT-UNSL PROICO 02-0820 and FONCYT PICT-201-0595.
Fil: Padrones, Marcelo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
Enzimas Pancreáticas
Aves
Corticosterona
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247634
Acceder
id CONICETDig_bef263d61a5ff59d2006aa023ff8fca8
oai_identifier_str oai:ri.conicet.gov.ar:11336/247634
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pancreatic enzyme activity in passer domesticus exposed to corticosteronePadrones, Marcelo NicolásCid, Fabricio DamianChediack, Juan GabrielEnzimas PancreáticasAvesCorticosteronahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In animals, it is well documented that stress produces metabolic alterations and an allostatic response (activation of the hypothalamicpituitary-adrenal axis and increase of corticosterone levels in blood) to maintain homeostasis in organisms. The objective of this work was to study the long-term corticosterone (CORT) effects on the digestive function in Passer domesticus. We focus especially on modifications in the activity of pancreatic enzymes, trypsin and chymotrypsin. To achieve our goal, 32 sparrows were exposed to different concentrations of CORT in drinking water (0, 20, 40 and 80 mg/l) for 72 hours emulating a long-term stress situation. After treatment, body weight was measured, blood extraction and subsequent removal of digestive organs (intestine, liver and pancreas) were performed at the same time (8:00 am), to avoid disturb by circadian/daily variation. The Heterophil/Lymphocyte (H/L) ratio was used as an indicator of stress in birds. The mass of pancreas was measured and homogenized. The enzymatic activity of trypsin and chymotrypsin were determined. Previously, chymotrypsinogen was activated by enterokinase for 60 min to measure chymotrypsin activity. We analyzed the mass-specific enzyme activities (μmol min−1 g wet tissue−1) using one-way ANOVA with Tukey post-hoc test (p < 0.05). We found a proportional increased inthe H/L index in concordance with CORT treatments. It was not found differences in the mass of pancreas and activity of enzymes (p > 0.05). Studies in chickens show a variation in digestive enzymes by corticosterone. However, our case in wild birds, we didn´t found effect of CORT on pancreatic enzyme activities. Previously, we found similar results with intestinal enzymes (carbohydrases and proteases). So, future research will be in direction to comparative studies in poultry and wild birds, under the same experimental design, to dilucidated mechanism involved in enzyme modulation by CORT. Supported by CyT-UNSL PROICO 02-0820 and FONCYT PICT-201-0595.Fil: Padrones, Marcelo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaXXIX Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247634Pancreatic enzyme activity in passer domesticus exposed to corticosterone; XXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2021; 79-79CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/http://reunion.sbcuyo.org.ar/wp-content/uploads/2021/12/Libro-de-Resu%CC%81menes.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:00Zoai:ri.conicet.gov.ar:11336/247634instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:00.357CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pancreatic enzyme activity in passer domesticus exposed to corticosterone
title Pancreatic enzyme activity in passer domesticus exposed to corticosterone
spellingShingle Pancreatic enzyme activity in passer domesticus exposed to corticosterone
Padrones, Marcelo Nicolás
Enzimas Pancreáticas
Aves
Corticosterona
title_short Pancreatic enzyme activity in passer domesticus exposed to corticosterone
title_full Pancreatic enzyme activity in passer domesticus exposed to corticosterone
title_fullStr Pancreatic enzyme activity in passer domesticus exposed to corticosterone
title_full_unstemmed Pancreatic enzyme activity in passer domesticus exposed to corticosterone
title_sort Pancreatic enzyme activity in passer domesticus exposed to corticosterone
dc.creator.none.fl_str_mv Padrones, Marcelo Nicolás
Cid, Fabricio Damian
Chediack, Juan Gabriel
author Padrones, Marcelo Nicolás
author_facet Padrones, Marcelo Nicolás
Cid, Fabricio Damian
Chediack, Juan Gabriel
author_role author
author2 Cid, Fabricio Damian
Chediack, Juan Gabriel
author2_role author
author
dc.subject.none.fl_str_mv Enzimas Pancreáticas
Aves
Corticosterona
topic Enzimas Pancreáticas
Aves
Corticosterona
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In animals, it is well documented that stress produces metabolic alterations and an allostatic response (activation of the hypothalamicpituitary-adrenal axis and increase of corticosterone levels in blood) to maintain homeostasis in organisms. The objective of this work was to study the long-term corticosterone (CORT) effects on the digestive function in Passer domesticus. We focus especially on modifications in the activity of pancreatic enzymes, trypsin and chymotrypsin. To achieve our goal, 32 sparrows were exposed to different concentrations of CORT in drinking water (0, 20, 40 and 80 mg/l) for 72 hours emulating a long-term stress situation. After treatment, body weight was measured, blood extraction and subsequent removal of digestive organs (intestine, liver and pancreas) were performed at the same time (8:00 am), to avoid disturb by circadian/daily variation. The Heterophil/Lymphocyte (H/L) ratio was used as an indicator of stress in birds. The mass of pancreas was measured and homogenized. The enzymatic activity of trypsin and chymotrypsin were determined. Previously, chymotrypsinogen was activated by enterokinase for 60 min to measure chymotrypsin activity. We analyzed the mass-specific enzyme activities (μmol min−1 g wet tissue−1) using one-way ANOVA with Tukey post-hoc test (p < 0.05). We found a proportional increased inthe H/L index in concordance with CORT treatments. It was not found differences in the mass of pancreas and activity of enzymes (p > 0.05). Studies in chickens show a variation in digestive enzymes by corticosterone. However, our case in wild birds, we didn´t found effect of CORT on pancreatic enzyme activities. Previously, we found similar results with intestinal enzymes (carbohydrases and proteases). So, future research will be in direction to comparative studies in poultry and wild birds, under the same experimental design, to dilucidated mechanism involved in enzyme modulation by CORT. Supported by CyT-UNSL PROICO 02-0820 and FONCYT PICT-201-0595.
Fil: Padrones, Marcelo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description In animals, it is well documented that stress produces metabolic alterations and an allostatic response (activation of the hypothalamicpituitary-adrenal axis and increase of corticosterone levels in blood) to maintain homeostasis in organisms. The objective of this work was to study the long-term corticosterone (CORT) effects on the digestive function in Passer domesticus. We focus especially on modifications in the activity of pancreatic enzymes, trypsin and chymotrypsin. To achieve our goal, 32 sparrows were exposed to different concentrations of CORT in drinking water (0, 20, 40 and 80 mg/l) for 72 hours emulating a long-term stress situation. After treatment, body weight was measured, blood extraction and subsequent removal of digestive organs (intestine, liver and pancreas) were performed at the same time (8:00 am), to avoid disturb by circadian/daily variation. The Heterophil/Lymphocyte (H/L) ratio was used as an indicator of stress in birds. The mass of pancreas was measured and homogenized. The enzymatic activity of trypsin and chymotrypsin were determined. Previously, chymotrypsinogen was activated by enterokinase for 60 min to measure chymotrypsin activity. We analyzed the mass-specific enzyme activities (μmol min−1 g wet tissue−1) using one-way ANOVA with Tukey post-hoc test (p < 0.05). We found a proportional increased inthe H/L index in concordance with CORT treatments. It was not found differences in the mass of pancreas and activity of enzymes (p > 0.05). Studies in chickens show a variation in digestive enzymes by corticosterone. However, our case in wild birds, we didn´t found effect of CORT on pancreatic enzyme activities. Previously, we found similar results with intestinal enzymes (carbohydrases and proteases). So, future research will be in direction to comparative studies in poultry and wild birds, under the same experimental design, to dilucidated mechanism involved in enzyme modulation by CORT. Supported by CyT-UNSL PROICO 02-0820 and FONCYT PICT-201-0595.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247634
Pancreatic enzyme activity in passer domesticus exposed to corticosterone; XXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2021; 79-79
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247634
identifier_str_mv Pancreatic enzyme activity in passer domesticus exposed to corticosterone; XXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2021; 79-79
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://reunion.sbcuyo.org.ar/wp-content/uploads/2021/12/Libro-de-Resu%CC%81menes.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269613779320832
score 13.13397

Publicaciones similares