Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera

Autores
Pilili, Juan Pablo; González, Norma Viviana; Molinari, Gabriela Beatriz; Reigosa, Miguel Antonio; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Effects of 18 commercial lots of fetal calf serum (FCS) after γ-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P < 0.05-0.001, Student's t-test) and 61.1% (P < 0.05-0.001, χ2 test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P < 0.05-0.001, χ2 test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.
Fil: Pilili, Juan Pablo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Molinari, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Reigosa, Miguel Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
COMET ASSAY
COMMERCIAL FETAL CALF SERUM
GAMMA IRRADIATION
MITOTIC INDEX
SCE ASSAY
STERILIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193032
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/193032
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf seraPilili, Juan PabloGonzález, Norma VivianaMolinari, Gabriela BeatrizReigosa, Miguel AntonioSoloneski, Sonia Maria ElsaLarramendy, Marcelo LuisCOMET ASSAYCOMMERCIAL FETAL CALF SERUMGAMMA IRRADIATIONMITOTIC INDEXSCE ASSAYSTERILIZATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Effects of 18 commercial lots of fetal calf serum (FCS) after γ-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P < 0.05-0.001, Student's t-test) and 61.1% (P < 0.05-0.001, χ2 test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P < 0.05-0.001, χ2 test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.Fil: Pilili, Juan Pablo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Molinari, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Reigosa, Miguel Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193032Pilili, Juan Pablo; González, Norma Viviana; Molinari, Gabriela Beatriz; Reigosa, Miguel Antonio; Soloneski, Sonia Maria Elsa; et al.; Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera; Academic Press Ltd - Elsevier Science Ltd; Biologicals; 38; 1; 1-2010; 135-1431045-1056CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1045105609001183info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biologicals.2009.08.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:44Zoai:ri.conicet.gov.ar:11336/193032instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:44.706CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
title Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
spellingShingle Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
Pilili, Juan Pablo
COMET ASSAY
COMMERCIAL FETAL CALF SERUM
GAMMA IRRADIATION
MITOTIC INDEX
SCE ASSAY
STERILIZATION
title_short Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
title_full Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
title_fullStr Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
title_full_unstemmed Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
title_sort Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
dc.creator.none.fl_str_mv Pilili, Juan Pablo
González, Norma Viviana
Molinari, Gabriela Beatriz
Reigosa, Miguel Antonio
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author Pilili, Juan Pablo
author_facet Pilili, Juan Pablo
González, Norma Viviana
Molinari, Gabriela Beatriz
Reigosa, Miguel Antonio
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author_role author
author2 González, Norma Viviana
Molinari, Gabriela Beatriz
Reigosa, Miguel Antonio
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv COMET ASSAY
COMMERCIAL FETAL CALF SERUM
GAMMA IRRADIATION
MITOTIC INDEX
SCE ASSAY
STERILIZATION
topic COMET ASSAY
COMMERCIAL FETAL CALF SERUM
GAMMA IRRADIATION
MITOTIC INDEX
SCE ASSAY
STERILIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Effects of 18 commercial lots of fetal calf serum (FCS) after γ-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P < 0.05-0.001, Student's t-test) and 61.1% (P < 0.05-0.001, χ2 test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P < 0.05-0.001, χ2 test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.
Fil: Pilili, Juan Pablo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Molinari, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Reigosa, Miguel Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Effects of 18 commercial lots of fetal calf serum (FCS) after γ-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P < 0.05-0.001, Student's t-test) and 61.1% (P < 0.05-0.001, χ2 test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P < 0.05-0.001, χ2 test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193032
Pilili, Juan Pablo; González, Norma Viviana; Molinari, Gabriela Beatriz; Reigosa, Miguel Antonio; Soloneski, Sonia Maria Elsa; et al.; Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera; Academic Press Ltd - Elsevier Science Ltd; Biologicals; 38; 1; 1-2010; 135-143
1045-1056
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193032
identifier_str_mv Pilili, Juan Pablo; González, Norma Viviana; Molinari, Gabriela Beatriz; Reigosa, Miguel Antonio; Soloneski, Sonia Maria Elsa; et al.; Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera; Academic Press Ltd - Elsevier Science Ltd; Biologicals; 38; 1; 1-2010; 135-143
1045-1056
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1045105609001183
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biologicals.2009.08.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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