New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)

Autores
Bessega, Cecilia Fabiana; Pometti, Carolina Luciana; Miller, Joe T.; Watts, Richard; Saidman, Beatriz Ofelia; Vilardi, Juan Cesar
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prosopis (Leguminosae) contains woody species of arid and semiarid areas of the Americas, Africa and Asia. There is considerable taxonomic confusion about the American section Algarobia, due largely to the phenotypic variation within species and the low genetic differentiation and frequent hybridization among species. Population genetic, conservation and evolutionary studies using molecular markers have been generated over the last few years in Prosopis. However, the lack of a sufficient number of SSR?s limits the conclusions that can be inferred. The only available microsatellite markers in Prosopis were six loci developed in P. chilensis. They revealed cross-species affinity when tested in five species of Algarobia and two of Strombocarpa sections. Although these markers proved to be useful loci for populations studies in P. alba at least 10 microsatellite loci are recommended to accurately estimate relatedness coefficients and diverse population parameters depending on parentage estimates. In the current study we developed additional SSR useful for population genetic analysis including effects of fragmentation on loss of diversity, fine scale population structure, breeding system and pollen dispersal, and detection of recent bottlenecks in P. alba and P. chilensis, the most important native forest resources among Argentinean ?algarrobos?. Also, these SSR will contribute to more accurate relationships estimates which may be used to in situ heritability estimates. Taking advantage of the high genetic similarity among Algarobia species and the observation of cross-amplification in several species of Prosopis we searched for possible microsatellite loci from a 454 sequence data from a bulk sample of American mesquites introduced to Australia. Then we tested their utility in these species and cross-amplification in five other Algarobia species.
Fil: Bessega, Cecilia Fabiana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;
Fil: Pometti, Carolina Luciana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;
Fil: Miller, Joe T.. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;
Fil: Watts, Richard. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;
Fil: Saidman, Beatriz Ofelia. Universidad de Buenos Aires; Argentina; Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina;
Fil: Vilardi, Juan Cesar. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;
Materia
microsatellite
Next generation sequencing data
Prosopis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/783

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network_name_str CONICET Digital (CONICET)
spelling New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)Bessega, Cecilia FabianaPometti, Carolina LucianaMiller, Joe T.Watts, RichardSaidman, Beatriz OfeliaVilardi, Juan CesarmicrosatelliteNext generation sequencing dataProsopishttps://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6Prosopis (Leguminosae) contains woody species of arid and semiarid areas of the Americas, Africa and Asia. There is considerable taxonomic confusion about the American section Algarobia, due largely to the phenotypic variation within species and the low genetic differentiation and frequent hybridization among species. Population genetic, conservation and evolutionary studies using molecular markers have been generated over the last few years in Prosopis. However, the lack of a sufficient number of SSR?s limits the conclusions that can be inferred. The only available microsatellite markers in Prosopis were six loci developed in P. chilensis. They revealed cross-species affinity when tested in five species of Algarobia and two of Strombocarpa sections. Although these markers proved to be useful loci for populations studies in P. alba at least 10 microsatellite loci are recommended to accurately estimate relatedness coefficients and diverse population parameters depending on parentage estimates. In the current study we developed additional SSR useful for population genetic analysis including effects of fragmentation on loss of diversity, fine scale population structure, breeding system and pollen dispersal, and detection of recent bottlenecks in P. alba and P. chilensis, the most important native forest resources among Argentinean ?algarrobos?. Also, these SSR will contribute to more accurate relationships estimates which may be used to in situ heritability estimates. Taking advantage of the high genetic similarity among Algarobia species and the observation of cross-amplification in several species of Prosopis we searched for possible microsatellite loci from a 454 sequence data from a bulk sample of American mesquites introduced to Australia. Then we tested their utility in these species and cross-amplification in five other Algarobia species.Fil: Bessega, Cecilia Fabiana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;Fil: Pometti, Carolina Luciana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;Fil: Miller, Joe T.. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;Fil: Watts, Richard. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;Fil: Saidman, Beatriz Ofelia. Universidad de Buenos Aires; Argentina; Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina;Fil: Vilardi, Juan Cesar. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;Botanical Soc Amer Inc2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/783Bessega, Cecilia Fabiana; Pometti, Carolina Luciana; Miller, Joe T.; Watts, Richard; Saidman, Beatriz Ofelia; et al.;New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae); Botanical Soc Amer Inc; Applications in Plant Sciences; 1; 5; 5-2013; 1-112168-0450enginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105034/info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/loi/appsinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:19Zoai:ri.conicet.gov.ar:11336/783instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:19.615CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
title New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
spellingShingle New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
Bessega, Cecilia Fabiana
microsatellite
Next generation sequencing data
Prosopis
title_short New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
title_full New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
title_fullStr New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
title_full_unstemmed New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
title_sort New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
dc.creator.none.fl_str_mv Bessega, Cecilia Fabiana
Pometti, Carolina Luciana
Miller, Joe T.
Watts, Richard
Saidman, Beatriz Ofelia
Vilardi, Juan Cesar
author Bessega, Cecilia Fabiana
author_facet Bessega, Cecilia Fabiana
Pometti, Carolina Luciana
Miller, Joe T.
Watts, Richard
Saidman, Beatriz Ofelia
Vilardi, Juan Cesar
author_role author
author2 Pometti, Carolina Luciana
Miller, Joe T.
Watts, Richard
Saidman, Beatriz Ofelia
Vilardi, Juan Cesar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv microsatellite
Next generation sequencing data
Prosopis
topic microsatellite
Next generation sequencing data
Prosopis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv Prosopis (Leguminosae) contains woody species of arid and semiarid areas of the Americas, Africa and Asia. There is considerable taxonomic confusion about the American section Algarobia, due largely to the phenotypic variation within species and the low genetic differentiation and frequent hybridization among species. Population genetic, conservation and evolutionary studies using molecular markers have been generated over the last few years in Prosopis. However, the lack of a sufficient number of SSR?s limits the conclusions that can be inferred. The only available microsatellite markers in Prosopis were six loci developed in P. chilensis. They revealed cross-species affinity when tested in five species of Algarobia and two of Strombocarpa sections. Although these markers proved to be useful loci for populations studies in P. alba at least 10 microsatellite loci are recommended to accurately estimate relatedness coefficients and diverse population parameters depending on parentage estimates. In the current study we developed additional SSR useful for population genetic analysis including effects of fragmentation on loss of diversity, fine scale population structure, breeding system and pollen dispersal, and detection of recent bottlenecks in P. alba and P. chilensis, the most important native forest resources among Argentinean ?algarrobos?. Also, these SSR will contribute to more accurate relationships estimates which may be used to in situ heritability estimates. Taking advantage of the high genetic similarity among Algarobia species and the observation of cross-amplification in several species of Prosopis we searched for possible microsatellite loci from a 454 sequence data from a bulk sample of American mesquites introduced to Australia. Then we tested their utility in these species and cross-amplification in five other Algarobia species.
Fil: Bessega, Cecilia Fabiana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;
Fil: Pometti, Carolina Luciana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;
Fil: Miller, Joe T.. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;
Fil: Watts, Richard. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;
Fil: Saidman, Beatriz Ofelia. Universidad de Buenos Aires; Argentina; Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina;
Fil: Vilardi, Juan Cesar. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;
description Prosopis (Leguminosae) contains woody species of arid and semiarid areas of the Americas, Africa and Asia. There is considerable taxonomic confusion about the American section Algarobia, due largely to the phenotypic variation within species and the low genetic differentiation and frequent hybridization among species. Population genetic, conservation and evolutionary studies using molecular markers have been generated over the last few years in Prosopis. However, the lack of a sufficient number of SSR?s limits the conclusions that can be inferred. The only available microsatellite markers in Prosopis were six loci developed in P. chilensis. They revealed cross-species affinity when tested in five species of Algarobia and two of Strombocarpa sections. Although these markers proved to be useful loci for populations studies in P. alba at least 10 microsatellite loci are recommended to accurately estimate relatedness coefficients and diverse population parameters depending on parentage estimates. In the current study we developed additional SSR useful for population genetic analysis including effects of fragmentation on loss of diversity, fine scale population structure, breeding system and pollen dispersal, and detection of recent bottlenecks in P. alba and P. chilensis, the most important native forest resources among Argentinean ?algarrobos?. Also, these SSR will contribute to more accurate relationships estimates which may be used to in situ heritability estimates. Taking advantage of the high genetic similarity among Algarobia species and the observation of cross-amplification in several species of Prosopis we searched for possible microsatellite loci from a 454 sequence data from a bulk sample of American mesquites introduced to Australia. Then we tested their utility in these species and cross-amplification in five other Algarobia species.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/783
Bessega, Cecilia Fabiana; Pometti, Carolina Luciana; Miller, Joe T.; Watts, Richard; Saidman, Beatriz Ofelia; et al.;New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae); Botanical Soc Amer Inc; Applications in Plant Sciences; 1; 5; 5-2013; 1-11
2168-0450
url http://hdl.handle.net/11336/783
identifier_str_mv Bessega, Cecilia Fabiana; Pometti, Carolina Luciana; Miller, Joe T.; Watts, Richard; Saidman, Beatriz Ofelia; et al.;New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae); Botanical Soc Amer Inc; Applications in Plant Sciences; 1; 5; 5-2013; 1-11
2168-0450
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105034/
info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/loi/apps
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Botanical Soc Amer Inc
publisher.none.fl_str_mv Botanical Soc Amer Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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