New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)
- Autores
- Bessega, Cecilia Fabiana; Pometti, Carolina Luciana; Miller, Joe T.; Watts, Richard; Saidman, Beatriz Ofelia; Vilardi, Juan Cesar
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Prosopis (Leguminosae) contains woody species of arid and semiarid areas of the Americas, Africa and Asia. There is considerable taxonomic confusion about the American section Algarobia, due largely to the phenotypic variation within species and the low genetic differentiation and frequent hybridization among species. Population genetic, conservation and evolutionary studies using molecular markers have been generated over the last few years in Prosopis. However, the lack of a sufficient number of SSR?s limits the conclusions that can be inferred. The only available microsatellite markers in Prosopis were six loci developed in P. chilensis. They revealed cross-species affinity when tested in five species of Algarobia and two of Strombocarpa sections. Although these markers proved to be useful loci for populations studies in P. alba at least 10 microsatellite loci are recommended to accurately estimate relatedness coefficients and diverse population parameters depending on parentage estimates. In the current study we developed additional SSR useful for population genetic analysis including effects of fragmentation on loss of diversity, fine scale population structure, breeding system and pollen dispersal, and detection of recent bottlenecks in P. alba and P. chilensis, the most important native forest resources among Argentinean ?algarrobos?. Also, these SSR will contribute to more accurate relationships estimates which may be used to in situ heritability estimates. Taking advantage of the high genetic similarity among Algarobia species and the observation of cross-amplification in several species of Prosopis we searched for possible microsatellite loci from a 454 sequence data from a bulk sample of American mesquites introduced to Australia. Then we tested their utility in these species and cross-amplification in five other Algarobia species.
Fil: Bessega, Cecilia Fabiana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;
Fil: Pometti, Carolina Luciana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;
Fil: Miller, Joe T.. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;
Fil: Watts, Richard. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;
Fil: Saidman, Beatriz Ofelia. Universidad de Buenos Aires; Argentina; Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina;
Fil: Vilardi, Juan Cesar. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina; - Materia
-
microsatellite
Next generation sequencing data
Prosopis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/783
Ver los metadatos del registro completo
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New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)Bessega, Cecilia FabianaPometti, Carolina LucianaMiller, Joe T.Watts, RichardSaidman, Beatriz OfeliaVilardi, Juan CesarmicrosatelliteNext generation sequencing dataProsopishttps://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6Prosopis (Leguminosae) contains woody species of arid and semiarid areas of the Americas, Africa and Asia. There is considerable taxonomic confusion about the American section Algarobia, due largely to the phenotypic variation within species and the low genetic differentiation and frequent hybridization among species. Population genetic, conservation and evolutionary studies using molecular markers have been generated over the last few years in Prosopis. However, the lack of a sufficient number of SSR?s limits the conclusions that can be inferred. The only available microsatellite markers in Prosopis were six loci developed in P. chilensis. They revealed cross-species affinity when tested in five species of Algarobia and two of Strombocarpa sections. Although these markers proved to be useful loci for populations studies in P. alba at least 10 microsatellite loci are recommended to accurately estimate relatedness coefficients and diverse population parameters depending on parentage estimates. In the current study we developed additional SSR useful for population genetic analysis including effects of fragmentation on loss of diversity, fine scale population structure, breeding system and pollen dispersal, and detection of recent bottlenecks in P. alba and P. chilensis, the most important native forest resources among Argentinean ?algarrobos?. Also, these SSR will contribute to more accurate relationships estimates which may be used to in situ heritability estimates. Taking advantage of the high genetic similarity among Algarobia species and the observation of cross-amplification in several species of Prosopis we searched for possible microsatellite loci from a 454 sequence data from a bulk sample of American mesquites introduced to Australia. Then we tested their utility in these species and cross-amplification in five other Algarobia species.Fil: Bessega, Cecilia Fabiana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;Fil: Pometti, Carolina Luciana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;Fil: Miller, Joe T.. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;Fil: Watts, Richard. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia;Fil: Saidman, Beatriz Ofelia. Universidad de Buenos Aires; Argentina; Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina;Fil: Vilardi, Juan Cesar. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina;Botanical Soc Amer Inc2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/783Bessega, Cecilia Fabiana; Pometti, Carolina Luciana; Miller, Joe T.; Watts, Richard; Saidman, Beatriz Ofelia; et al.;New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae); Botanical Soc Amer Inc; Applications in Plant Sciences; 1; 5; 5-2013; 1-112168-0450enginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105034/info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/loi/appsinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:19Zoai:ri.conicet.gov.ar:11336/783instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:19.615CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae) |
title |
New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae) |
spellingShingle |
New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae) Bessega, Cecilia Fabiana microsatellite Next generation sequencing data Prosopis |
title_short |
New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae) |
title_full |
New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae) |
title_fullStr |
New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae) |
title_full_unstemmed |
New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae) |
title_sort |
New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae) |
dc.creator.none.fl_str_mv |
Bessega, Cecilia Fabiana Pometti, Carolina Luciana Miller, Joe T. Watts, Richard Saidman, Beatriz Ofelia Vilardi, Juan Cesar |
author |
Bessega, Cecilia Fabiana |
author_facet |
Bessega, Cecilia Fabiana Pometti, Carolina Luciana Miller, Joe T. Watts, Richard Saidman, Beatriz Ofelia Vilardi, Juan Cesar |
author_role |
author |
author2 |
Pometti, Carolina Luciana Miller, Joe T. Watts, Richard Saidman, Beatriz Ofelia Vilardi, Juan Cesar |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
microsatellite Next generation sequencing data Prosopis |
topic |
microsatellite Next generation sequencing data Prosopis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 |
dc.description.none.fl_txt_mv |
Prosopis (Leguminosae) contains woody species of arid and semiarid areas of the Americas, Africa and Asia. There is considerable taxonomic confusion about the American section Algarobia, due largely to the phenotypic variation within species and the low genetic differentiation and frequent hybridization among species. Population genetic, conservation and evolutionary studies using molecular markers have been generated over the last few years in Prosopis. However, the lack of a sufficient number of SSR?s limits the conclusions that can be inferred. The only available microsatellite markers in Prosopis were six loci developed in P. chilensis. They revealed cross-species affinity when tested in five species of Algarobia and two of Strombocarpa sections. Although these markers proved to be useful loci for populations studies in P. alba at least 10 microsatellite loci are recommended to accurately estimate relatedness coefficients and diverse population parameters depending on parentage estimates. In the current study we developed additional SSR useful for population genetic analysis including effects of fragmentation on loss of diversity, fine scale population structure, breeding system and pollen dispersal, and detection of recent bottlenecks in P. alba and P. chilensis, the most important native forest resources among Argentinean ?algarrobos?. Also, these SSR will contribute to more accurate relationships estimates which may be used to in situ heritability estimates. Taking advantage of the high genetic similarity among Algarobia species and the observation of cross-amplification in several species of Prosopis we searched for possible microsatellite loci from a 454 sequence data from a bulk sample of American mesquites introduced to Australia. Then we tested their utility in these species and cross-amplification in five other Algarobia species. Fil: Bessega, Cecilia Fabiana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina; Fil: Pometti, Carolina Luciana. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina; Fil: Miller, Joe T.. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia; Fil: Watts, Richard. CSIRO Plant Industry. Centre for Australian National Biodiversity Research; Australia; Fil: Saidman, Beatriz Ofelia. Universidad de Buenos Aires; Argentina; Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Fil: Vilardi, Juan Cesar. Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Bs. As; Argentina; Universidad de Buenos Aires; Argentina; |
description |
Prosopis (Leguminosae) contains woody species of arid and semiarid areas of the Americas, Africa and Asia. There is considerable taxonomic confusion about the American section Algarobia, due largely to the phenotypic variation within species and the low genetic differentiation and frequent hybridization among species. Population genetic, conservation and evolutionary studies using molecular markers have been generated over the last few years in Prosopis. However, the lack of a sufficient number of SSR?s limits the conclusions that can be inferred. The only available microsatellite markers in Prosopis were six loci developed in P. chilensis. They revealed cross-species affinity when tested in five species of Algarobia and two of Strombocarpa sections. Although these markers proved to be useful loci for populations studies in P. alba at least 10 microsatellite loci are recommended to accurately estimate relatedness coefficients and diverse population parameters depending on parentage estimates. In the current study we developed additional SSR useful for population genetic analysis including effects of fragmentation on loss of diversity, fine scale population structure, breeding system and pollen dispersal, and detection of recent bottlenecks in P. alba and P. chilensis, the most important native forest resources among Argentinean ?algarrobos?. Also, these SSR will contribute to more accurate relationships estimates which may be used to in situ heritability estimates. Taking advantage of the high genetic similarity among Algarobia species and the observation of cross-amplification in several species of Prosopis we searched for possible microsatellite loci from a 454 sequence data from a bulk sample of American mesquites introduced to Australia. Then we tested their utility in these species and cross-amplification in five other Algarobia species. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/783 Bessega, Cecilia Fabiana; Pometti, Carolina Luciana; Miller, Joe T.; Watts, Richard; Saidman, Beatriz Ofelia; et al.;New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae); Botanical Soc Amer Inc; Applications in Plant Sciences; 1; 5; 5-2013; 1-11 2168-0450 |
url |
http://hdl.handle.net/11336/783 |
identifier_str_mv |
Bessega, Cecilia Fabiana; Pometti, Carolina Luciana; Miller, Joe T.; Watts, Richard; Saidman, Beatriz Ofelia; et al.;New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae); Botanical Soc Amer Inc; Applications in Plant Sciences; 1; 5; 5-2013; 1-11 2168-0450 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105034/ info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/loi/apps |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Botanical Soc Amer Inc |
publisher.none.fl_str_mv |
Botanical Soc Amer Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269023910232064 |
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13.13397 |