Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors

Autores
Ulloa, Ricardo; Moya Beltrán, Ana; Rojas Villalobos, Camila; Nuñez, Harold; Chiacchiarini, Patricia Alejandra; Donati, Edgardo Ruben; Giaveno, Alejandra; Quatrini, Raquel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Extreme acidophiles play central roles in the geochemical cycling of diverse elements in low pH environments. This has been harnessed in biotechnologies such as biomining, where microorganisms facilitate the recovery of economically important metals such as gold. By generating both extreme acidity and a chemical oxidant (ferric iron) many species of prokaryotes that thrive in low pH environments not only catalyze mineral dissolution but also trigger both community and individual level adaptive changes. These changes vary in extent and direction depending on the ore mineralogy, water availability and local climate. The use of indigenous versus introduced microbial consortia in biomining practices is still a matter of debate. Yet, indigenous microbial consortia colonizing sulfidic ores that have been domesticated, i.e., selected for their ability to survive under specific polyextreme conditions, are claimed to outperform un-adapted foreign consortia. Despite this, little is known on the domestication of acidic microbial communities and the changes elicited in their members. In this study, high resolution targeted metagenomic techniques were used to analyze the changes occurring in the community structure of local microbial consortia acclimated to growing under extreme acidic conditions and adapted to endure the conditions imposed by the target mineral during biooxidation of a gold concentrate in an airlift reactor over a period of 2 years. The results indicated that operative conditions evolving through biooxidation of the mineral concentrate exerted strong selective pressures that, early on, purge biodiversity in favor of a few Acidithiobacillus spp. over other iron oxidizing acidophiles. Metagenomic analysis of the domesticated consortium present at the end of the adaptation experiment enabled reconstruction of the RVS1-MAG, a novel representative of Acidithiobacillus ferrooxidans from the Andacollo gold mineral district. Comparative genomic analysis performed with this genome draft revealed a net enrichment of gene functions related to heavy metal transport and stress management that are likely to play a significant role in adaptation and survival to adverse conditions experienced by these acidophiles during growth in presence of gold concentrates.
Fil: Ulloa, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Moya Beltrán, Ana. Fundación Ciencia & Vida; Chile. Universidad Andrés Bello; Chile
Fil: Rojas Villalobos, Camila. Fundación Ciencia & Vida; Chile
Fil: Nuñez, Harold. Fundación Ciencia & Vida; Chile
Fil: Chiacchiarini, Patricia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Giaveno, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Quatrini, Raquel. Fundación Ciencia & Vida; Chile. Millennium Nucleus in the Biology of the Intestinal Microbiota; Chile
Materia
ACIDITHIOBACILLUS
ACIDOPHILES
ADAPTATION
CONSORTIA
DOMESTICATION
METAGENOME DERIVED ASSEMBLY
TARGETED METAGENOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/122711
Acceder
id CONICETDig_be68480f59d5fee5c75da9967ed4e477
oai_identifier_str oai:ri.conicet.gov.ar:11336/122711
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactorsUlloa, RicardoMoya Beltrán, AnaRojas Villalobos, CamilaNuñez, HaroldChiacchiarini, Patricia AlejandraDonati, Edgardo RubenGiaveno, AlejandraQuatrini, RaquelACIDITHIOBACILLUSACIDOPHILESADAPTATIONCONSORTIADOMESTICATIONMETAGENOME DERIVED ASSEMBLYTARGETED METAGENOMICShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Extreme acidophiles play central roles in the geochemical cycling of diverse elements in low pH environments. This has been harnessed in biotechnologies such as biomining, where microorganisms facilitate the recovery of economically important metals such as gold. By generating both extreme acidity and a chemical oxidant (ferric iron) many species of prokaryotes that thrive in low pH environments not only catalyze mineral dissolution but also trigger both community and individual level adaptive changes. These changes vary in extent and direction depending on the ore mineralogy, water availability and local climate. The use of indigenous versus introduced microbial consortia in biomining practices is still a matter of debate. Yet, indigenous microbial consortia colonizing sulfidic ores that have been domesticated, i.e., selected for their ability to survive under specific polyextreme conditions, are claimed to outperform un-adapted foreign consortia. Despite this, little is known on the domestication of acidic microbial communities and the changes elicited in their members. In this study, high resolution targeted metagenomic techniques were used to analyze the changes occurring in the community structure of local microbial consortia acclimated to growing under extreme acidic conditions and adapted to endure the conditions imposed by the target mineral during biooxidation of a gold concentrate in an airlift reactor over a period of 2 years. The results indicated that operative conditions evolving through biooxidation of the mineral concentrate exerted strong selective pressures that, early on, purge biodiversity in favor of a few Acidithiobacillus spp. over other iron oxidizing acidophiles. Metagenomic analysis of the domesticated consortium present at the end of the adaptation experiment enabled reconstruction of the RVS1-MAG, a novel representative of Acidithiobacillus ferrooxidans from the Andacollo gold mineral district. Comparative genomic analysis performed with this genome draft revealed a net enrichment of gene functions related to heavy metal transport and stress management that are likely to play a significant role in adaptation and survival to adverse conditions experienced by these acidophiles during growth in presence of gold concentrates.Fil: Ulloa, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; ArgentinaFil: Moya Beltrán, Ana. Fundación Ciencia & Vida; Chile. Universidad Andrés Bello; ChileFil: Rojas Villalobos, Camila. Fundación Ciencia & Vida; ChileFil: Nuñez, Harold. Fundación Ciencia & Vida; ChileFil: Chiacchiarini, Patricia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; ArgentinaFil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Giaveno, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; ArgentinaFil: Quatrini, Raquel. Fundación Ciencia & Vida; Chile. Millennium Nucleus in the Biology of the Intestinal Microbiota; ChileFrontiers Media S.A.2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122711Ulloa, Ricardo; Moya Beltrán, Ana; Rojas Villalobos, Camila; Nuñez, Harold; Chiacchiarini, Patricia Alejandra; et al.; Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors; Frontiers Media S.A.; Frontiers in Microbiology; 10; 60; 4-2019; 1-141664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.00060/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.00060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:21:42Zoai:ri.conicet.gov.ar:11336/122711instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:21:42.763CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
title Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
spellingShingle Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
Ulloa, Ricardo
ACIDITHIOBACILLUS
ACIDOPHILES
ADAPTATION
CONSORTIA
DOMESTICATION
METAGENOME DERIVED ASSEMBLY
TARGETED METAGENOMICS
title_short Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
title_full Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
title_fullStr Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
title_full_unstemmed Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
title_sort Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
dc.creator.none.fl_str_mv Ulloa, Ricardo
Moya Beltrán, Ana
Rojas Villalobos, Camila
Nuñez, Harold
Chiacchiarini, Patricia Alejandra
Donati, Edgardo Ruben
Giaveno, Alejandra
Quatrini, Raquel
author Ulloa, Ricardo
author_facet Ulloa, Ricardo
Moya Beltrán, Ana
Rojas Villalobos, Camila
Nuñez, Harold
Chiacchiarini, Patricia Alejandra
Donati, Edgardo Ruben
Giaveno, Alejandra
Quatrini, Raquel
author_role author
author2 Moya Beltrán, Ana
Rojas Villalobos, Camila
Nuñez, Harold
Chiacchiarini, Patricia Alejandra
Donati, Edgardo Ruben
Giaveno, Alejandra
Quatrini, Raquel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACIDITHIOBACILLUS
ACIDOPHILES
ADAPTATION
CONSORTIA
DOMESTICATION
METAGENOME DERIVED ASSEMBLY
TARGETED METAGENOMICS
topic ACIDITHIOBACILLUS
ACIDOPHILES
ADAPTATION
CONSORTIA
DOMESTICATION
METAGENOME DERIVED ASSEMBLY
TARGETED METAGENOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Extreme acidophiles play central roles in the geochemical cycling of diverse elements in low pH environments. This has been harnessed in biotechnologies such as biomining, where microorganisms facilitate the recovery of economically important metals such as gold. By generating both extreme acidity and a chemical oxidant (ferric iron) many species of prokaryotes that thrive in low pH environments not only catalyze mineral dissolution but also trigger both community and individual level adaptive changes. These changes vary in extent and direction depending on the ore mineralogy, water availability and local climate. The use of indigenous versus introduced microbial consortia in biomining practices is still a matter of debate. Yet, indigenous microbial consortia colonizing sulfidic ores that have been domesticated, i.e., selected for their ability to survive under specific polyextreme conditions, are claimed to outperform un-adapted foreign consortia. Despite this, little is known on the domestication of acidic microbial communities and the changes elicited in their members. In this study, high resolution targeted metagenomic techniques were used to analyze the changes occurring in the community structure of local microbial consortia acclimated to growing under extreme acidic conditions and adapted to endure the conditions imposed by the target mineral during biooxidation of a gold concentrate in an airlift reactor over a period of 2 years. The results indicated that operative conditions evolving through biooxidation of the mineral concentrate exerted strong selective pressures that, early on, purge biodiversity in favor of a few Acidithiobacillus spp. over other iron oxidizing acidophiles. Metagenomic analysis of the domesticated consortium present at the end of the adaptation experiment enabled reconstruction of the RVS1-MAG, a novel representative of Acidithiobacillus ferrooxidans from the Andacollo gold mineral district. Comparative genomic analysis performed with this genome draft revealed a net enrichment of gene functions related to heavy metal transport and stress management that are likely to play a significant role in adaptation and survival to adverse conditions experienced by these acidophiles during growth in presence of gold concentrates.
Fil: Ulloa, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Moya Beltrán, Ana. Fundación Ciencia & Vida; Chile. Universidad Andrés Bello; Chile
Fil: Rojas Villalobos, Camila. Fundación Ciencia & Vida; Chile
Fil: Nuñez, Harold. Fundación Ciencia & Vida; Chile
Fil: Chiacchiarini, Patricia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Giaveno, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Quatrini, Raquel. Fundación Ciencia & Vida; Chile. Millennium Nucleus in the Biology of the Intestinal Microbiota; Chile
description Extreme acidophiles play central roles in the geochemical cycling of diverse elements in low pH environments. This has been harnessed in biotechnologies such as biomining, where microorganisms facilitate the recovery of economically important metals such as gold. By generating both extreme acidity and a chemical oxidant (ferric iron) many species of prokaryotes that thrive in low pH environments not only catalyze mineral dissolution but also trigger both community and individual level adaptive changes. These changes vary in extent and direction depending on the ore mineralogy, water availability and local climate. The use of indigenous versus introduced microbial consortia in biomining practices is still a matter of debate. Yet, indigenous microbial consortia colonizing sulfidic ores that have been domesticated, i.e., selected for their ability to survive under specific polyextreme conditions, are claimed to outperform un-adapted foreign consortia. Despite this, little is known on the domestication of acidic microbial communities and the changes elicited in their members. In this study, high resolution targeted metagenomic techniques were used to analyze the changes occurring in the community structure of local microbial consortia acclimated to growing under extreme acidic conditions and adapted to endure the conditions imposed by the target mineral during biooxidation of a gold concentrate in an airlift reactor over a period of 2 years. The results indicated that operative conditions evolving through biooxidation of the mineral concentrate exerted strong selective pressures that, early on, purge biodiversity in favor of a few Acidithiobacillus spp. over other iron oxidizing acidophiles. Metagenomic analysis of the domesticated consortium present at the end of the adaptation experiment enabled reconstruction of the RVS1-MAG, a novel representative of Acidithiobacillus ferrooxidans from the Andacollo gold mineral district. Comparative genomic analysis performed with this genome draft revealed a net enrichment of gene functions related to heavy metal transport and stress management that are likely to play a significant role in adaptation and survival to adverse conditions experienced by these acidophiles during growth in presence of gold concentrates.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/122711
Ulloa, Ricardo; Moya Beltrán, Ana; Rojas Villalobos, Camila; Nuñez, Harold; Chiacchiarini, Patricia Alejandra; et al.; Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors; Frontiers Media S.A.; Frontiers in Microbiology; 10; 60; 4-2019; 1-14
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/122711
identifier_str_mv Ulloa, Ricardo; Moya Beltrán, Ana; Rojas Villalobos, Camila; Nuñez, Harold; Chiacchiarini, Patricia Alejandra; et al.; Domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors; Frontiers Media S.A.; Frontiers in Microbiology; 10; 60; 4-2019; 1-14
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.00060/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.00060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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