Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch

Autores
Marquez, Lorenzo; Hernandez, Adrián J.; Carvalho, Edison S. M.; Morales, Gabriel Alejandro; Dantagnan, Patricio; Díaz, Manuel; Moyano, Francisco Javier
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.
Fil: Marquez, Lorenzo. Universidad Catolica de Temuco.; Chile
Fil: Hernandez, Adrián J.. Universidad Catolica de Temuco.; Chile
Fil: Carvalho, Edison S. M.. Universidade de Cabo Verde; Cabo Verde
Fil: Morales, Gabriel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Dantagnan, Patricio. Universidad Catolica de Temuco.; Chile
Fil: Díaz, Manuel. Universidad de Almería; España
Fil: Moyano, Francisco Javier. Universidad de Almería; España
Materia
COHO SALMON
ENZYMATIC ACTIVITY
GASTRIC PROTEASES
IONIC STRENGTH
ONCORHYNCHUS KISUTCH
PH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147489
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutchMarquez, LorenzoHernandez, Adrián J.Carvalho, Edison S. M.Morales, Gabriel AlejandroDantagnan, PatricioDíaz, ManuelMoyano, Francisco JavierCOHO SALMONENZYMATIC ACTIVITYGASTRIC PROTEASESIONIC STRENGTHONCORHYNCHUS KISUTCHPHhttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4The activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.Fil: Marquez, Lorenzo. Universidad Catolica de Temuco.; ChileFil: Hernandez, Adrián J.. Universidad Catolica de Temuco.; ChileFil: Carvalho, Edison S. M.. Universidade de Cabo Verde; Cabo VerdeFil: Morales, Gabriel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Dantagnan, Patricio. Universidad Catolica de Temuco.; ChileFil: Díaz, Manuel. Universidad de Almería; EspañaFil: Moyano, Francisco Javier. Universidad de Almería; EspañaPontificia Universidad Católica de Valparaíso2019-11-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147489Marquez, Lorenzo; Hernandez, Adrián J.; Carvalho, Edison S. M.; Morales, Gabriel Alejandro; Dantagnan, Patricio; et al.; Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch; Pontificia Universidad Católica de Valparaíso; Latin American Journal of Aquatic Research; 47; 5; 7-11-2019; 860-8640718-560XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://lajar.ucv.cl/index.php/rlajar/article/view/vol47-issue5-fulltext-16info:eu-repo/semantics/altIdentifier/doi/10.3856/vol47-issue5-fulltext-16info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:01Zoai:ri.conicet.gov.ar:11336/147489instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:01.512CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
title Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
spellingShingle Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
Marquez, Lorenzo
COHO SALMON
ENZYMATIC ACTIVITY
GASTRIC PROTEASES
IONIC STRENGTH
ONCORHYNCHUS KISUTCH
PH
title_short Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
title_full Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
title_fullStr Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
title_full_unstemmed Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
title_sort Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
dc.creator.none.fl_str_mv Marquez, Lorenzo
Hernandez, Adrián J.
Carvalho, Edison S. M.
Morales, Gabriel Alejandro
Dantagnan, Patricio
Díaz, Manuel
Moyano, Francisco Javier
author Marquez, Lorenzo
author_facet Marquez, Lorenzo
Hernandez, Adrián J.
Carvalho, Edison S. M.
Morales, Gabriel Alejandro
Dantagnan, Patricio
Díaz, Manuel
Moyano, Francisco Javier
author_role author
author2 Hernandez, Adrián J.
Carvalho, Edison S. M.
Morales, Gabriel Alejandro
Dantagnan, Patricio
Díaz, Manuel
Moyano, Francisco Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv COHO SALMON
ENZYMATIC ACTIVITY
GASTRIC PROTEASES
IONIC STRENGTH
ONCORHYNCHUS KISUTCH
PH
topic COHO SALMON
ENZYMATIC ACTIVITY
GASTRIC PROTEASES
IONIC STRENGTH
ONCORHYNCHUS KISUTCH
PH
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.
Fil: Marquez, Lorenzo. Universidad Catolica de Temuco.; Chile
Fil: Hernandez, Adrián J.. Universidad Catolica de Temuco.; Chile
Fil: Carvalho, Edison S. M.. Universidade de Cabo Verde; Cabo Verde
Fil: Morales, Gabriel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Dantagnan, Patricio. Universidad Catolica de Temuco.; Chile
Fil: Díaz, Manuel. Universidad de Almería; España
Fil: Moyano, Francisco Javier. Universidad de Almería; España
description The activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147489
Marquez, Lorenzo; Hernandez, Adrián J.; Carvalho, Edison S. M.; Morales, Gabriel Alejandro; Dantagnan, Patricio; et al.; Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch; Pontificia Universidad Católica de Valparaíso; Latin American Journal of Aquatic Research; 47; 5; 7-11-2019; 860-864
0718-560X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147489
identifier_str_mv Marquez, Lorenzo; Hernandez, Adrián J.; Carvalho, Edison S. M.; Morales, Gabriel Alejandro; Dantagnan, Patricio; et al.; Effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch; Pontificia Universidad Católica de Valparaíso; Latin American Journal of Aquatic Research; 47; 5; 7-11-2019; 860-864
0718-560X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://lajar.ucv.cl/index.php/rlajar/article/view/vol47-issue5-fulltext-16
info:eu-repo/semantics/altIdentifier/doi/10.3856/vol47-issue5-fulltext-16
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
publisher.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980560666886144
score 12.993085

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