Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation

Autores
Kalinka, Julia; Hachmeister, Marie; Geraci, Jennifer; Sordelli, Daniel Oscar; Hansen, Uwe; Niemann, Silke; Oetermann, Sylvia; Peters, Georg; Löffler, Bettina; Tuchscherr, Lorena Paola Nelly
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Osteomyelitis is a severe inflammatory disease of the bone that is mainly caused by Staphylococcus aureus. Particularly, bone infections are difficult to treat and can develop into a chronic course with a high relapsing rate despite of antimicrobial treatments. The complex interaction of staphylococci with osseous tissue and the bacterial ability to invade host cells are thought to determine the severity of infection. Yet, defined bacterial virulence factors responsible for the pathogenesis of osteomyelitis have not been clearly identified. The aim of this study was to detect S. aureus virulence factors that are associated with osteomyelitis and contribute to a chronic course of infection. To this purpose, we collected 41 S. aureus isolates, each 11 from acute osteomyelitis (infection period less than 2 months), 10 from chronic osteomyelitis (infection period more than 12 months), 10 from sepsis and 10 from nasal colonization. All isolates were analyzed for gene expression and in functional in-vitro systems. Adhesion assays to bone matrix revealed that all isolates equally bound to matrix structures, but invasion assays in human osteoblasts showed a high invasive capacity of chronic osteomyelitis isolates. The high invasion rate could not be explained by defined adhesins, as all infecting strains expressed a multitude of adhesins that act together and determine the level of adhesion. Following host cell invasion isolates from chronic osteomyelitis induced less cytotoxicity than all other isolates and a higher percentage of Small-colony-variant (SCV)-formation, which represents an adaptation mechanism during long-term persistence. Isolates from acute and chronic osteomyelitis strongly produced biofilm and highly expressed agr and sarA that regulate secreted virulence factors and induced an inflammatory response in osteoblasts. In conclusion, chronic osteomyelitis isolates were characterized by a high host cell invasion rate, low cytotoxicity and the ability to persist and adapt within osteoblasts. Furthermore, isolates from both acute and chronic osteomyelitis strongly produced biofilm and induced high levels of host cell inflammation, which may explain tissue destruction and bone deformation observed as typical complications of long-lasting bone infections.
Fil: Kalinka, Julia. University Hospital of Münster; Alemania
Fil: Hachmeister, Marie. University Hospital of Münster; Alemania
Fil: Geraci, Jennifer. University Hospital of Münster; Alemania. Jena University Hospital; Alemania
Fil: Sordelli, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Hansen, Uwe. University Hospital of Münster; Alemania
Fil: Niemann, Silke. University Hospital of Münster; Alemania
Fil: Oetermann, Sylvia. University Hospital of Münster; Alemania
Fil: Peters, Georg. University Hospital of Münster; Alemania
Fil: Löffler, Bettina. University Hospital of Münster; Alemania. Jena University Hospital; Alemania
Fil: Tuchscherr, Lorena Paola Nelly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University Hospital of Münster; Alemania. Jena University Hospital; Alemania
Materia
ADHESINS
CHRONIC INFECTIONS
HOST CELL INVASION
INTRACELLULAR PERSISTENCE
S. AUREUS OSTEOMYELITIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94483
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94483
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammationKalinka, JuliaHachmeister, MarieGeraci, JenniferSordelli, Daniel OscarHansen, UweNiemann, SilkeOetermann, SylviaPeters, GeorgLöffler, BettinaTuchscherr, Lorena Paola NellyADHESINSCHRONIC INFECTIONSHOST CELL INVASIONINTRACELLULAR PERSISTENCES. AUREUS OSTEOMYELITIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Osteomyelitis is a severe inflammatory disease of the bone that is mainly caused by Staphylococcus aureus. Particularly, bone infections are difficult to treat and can develop into a chronic course with a high relapsing rate despite of antimicrobial treatments. The complex interaction of staphylococci with osseous tissue and the bacterial ability to invade host cells are thought to determine the severity of infection. Yet, defined bacterial virulence factors responsible for the pathogenesis of osteomyelitis have not been clearly identified. The aim of this study was to detect S. aureus virulence factors that are associated with osteomyelitis and contribute to a chronic course of infection. To this purpose, we collected 41 S. aureus isolates, each 11 from acute osteomyelitis (infection period less than 2 months), 10 from chronic osteomyelitis (infection period more than 12 months), 10 from sepsis and 10 from nasal colonization. All isolates were analyzed for gene expression and in functional in-vitro systems. Adhesion assays to bone matrix revealed that all isolates equally bound to matrix structures, but invasion assays in human osteoblasts showed a high invasive capacity of chronic osteomyelitis isolates. The high invasion rate could not be explained by defined adhesins, as all infecting strains expressed a multitude of adhesins that act together and determine the level of adhesion. Following host cell invasion isolates from chronic osteomyelitis induced less cytotoxicity than all other isolates and a higher percentage of Small-colony-variant (SCV)-formation, which represents an adaptation mechanism during long-term persistence. Isolates from acute and chronic osteomyelitis strongly produced biofilm and highly expressed agr and sarA that regulate secreted virulence factors and induced an inflammatory response in osteoblasts. In conclusion, chronic osteomyelitis isolates were characterized by a high host cell invasion rate, low cytotoxicity and the ability to persist and adapt within osteoblasts. Furthermore, isolates from both acute and chronic osteomyelitis strongly produced biofilm and induced high levels of host cell inflammation, which may explain tissue destruction and bone deformation observed as typical complications of long-lasting bone infections.Fil: Kalinka, Julia. University Hospital of Münster; AlemaniaFil: Hachmeister, Marie. University Hospital of Münster; AlemaniaFil: Geraci, Jennifer. University Hospital of Münster; Alemania. Jena University Hospital; AlemaniaFil: Sordelli, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaFil: Hansen, Uwe. University Hospital of Münster; AlemaniaFil: Niemann, Silke. University Hospital of Münster; AlemaniaFil: Oetermann, Sylvia. University Hospital of Münster; AlemaniaFil: Peters, Georg. University Hospital of Münster; AlemaniaFil: Löffler, Bettina. University Hospital of Münster; Alemania. Jena University Hospital; AlemaniaFil: Tuchscherr, Lorena Paola Nelly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University Hospital of Münster; Alemania. Jena University Hospital; AlemaniaElsevier Gmbh2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94483Kalinka, Julia; Hachmeister, Marie; Geraci, Jennifer; Sordelli, Daniel Oscar; Hansen, Uwe; et al.; Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation; Elsevier Gmbh; International Journal of Medical Microbiology (print); 304; 8; 11-2014; 1038-10491438-4221CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1438422114000952info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijmm.2014.07.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:56Zoai:ri.conicet.gov.ar:11336/94483instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:57.081CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation
title Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation
spellingShingle Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation
Kalinka, Julia
ADHESINS
CHRONIC INFECTIONS
HOST CELL INVASION
INTRACELLULAR PERSISTENCE
S. AUREUS OSTEOMYELITIS
title_short Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation
title_full Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation
title_fullStr Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation
title_full_unstemmed Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation
title_sort Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation
dc.creator.none.fl_str_mv Kalinka, Julia
Hachmeister, Marie
Geraci, Jennifer
Sordelli, Daniel Oscar
Hansen, Uwe
Niemann, Silke
Oetermann, Sylvia
Peters, Georg
Löffler, Bettina
Tuchscherr, Lorena Paola Nelly
author Kalinka, Julia
author_facet Kalinka, Julia
Hachmeister, Marie
Geraci, Jennifer
Sordelli, Daniel Oscar
Hansen, Uwe
Niemann, Silke
Oetermann, Sylvia
Peters, Georg
Löffler, Bettina
Tuchscherr, Lorena Paola Nelly
author_role author
author2 Hachmeister, Marie
Geraci, Jennifer
Sordelli, Daniel Oscar
Hansen, Uwe
Niemann, Silke
Oetermann, Sylvia
Peters, Georg
Löffler, Bettina
Tuchscherr, Lorena Paola Nelly
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ADHESINS
CHRONIC INFECTIONS
HOST CELL INVASION
INTRACELLULAR PERSISTENCE
S. AUREUS OSTEOMYELITIS
topic ADHESINS
CHRONIC INFECTIONS
HOST CELL INVASION
INTRACELLULAR PERSISTENCE
S. AUREUS OSTEOMYELITIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Osteomyelitis is a severe inflammatory disease of the bone that is mainly caused by Staphylococcus aureus. Particularly, bone infections are difficult to treat and can develop into a chronic course with a high relapsing rate despite of antimicrobial treatments. The complex interaction of staphylococci with osseous tissue and the bacterial ability to invade host cells are thought to determine the severity of infection. Yet, defined bacterial virulence factors responsible for the pathogenesis of osteomyelitis have not been clearly identified. The aim of this study was to detect S. aureus virulence factors that are associated with osteomyelitis and contribute to a chronic course of infection. To this purpose, we collected 41 S. aureus isolates, each 11 from acute osteomyelitis (infection period less than 2 months), 10 from chronic osteomyelitis (infection period more than 12 months), 10 from sepsis and 10 from nasal colonization. All isolates were analyzed for gene expression and in functional in-vitro systems. Adhesion assays to bone matrix revealed that all isolates equally bound to matrix structures, but invasion assays in human osteoblasts showed a high invasive capacity of chronic osteomyelitis isolates. The high invasion rate could not be explained by defined adhesins, as all infecting strains expressed a multitude of adhesins that act together and determine the level of adhesion. Following host cell invasion isolates from chronic osteomyelitis induced less cytotoxicity than all other isolates and a higher percentage of Small-colony-variant (SCV)-formation, which represents an adaptation mechanism during long-term persistence. Isolates from acute and chronic osteomyelitis strongly produced biofilm and highly expressed agr and sarA that regulate secreted virulence factors and induced an inflammatory response in osteoblasts. In conclusion, chronic osteomyelitis isolates were characterized by a high host cell invasion rate, low cytotoxicity and the ability to persist and adapt within osteoblasts. Furthermore, isolates from both acute and chronic osteomyelitis strongly produced biofilm and induced high levels of host cell inflammation, which may explain tissue destruction and bone deformation observed as typical complications of long-lasting bone infections.
Fil: Kalinka, Julia. University Hospital of Münster; Alemania
Fil: Hachmeister, Marie. University Hospital of Münster; Alemania
Fil: Geraci, Jennifer. University Hospital of Münster; Alemania. Jena University Hospital; Alemania
Fil: Sordelli, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Hansen, Uwe. University Hospital of Münster; Alemania
Fil: Niemann, Silke. University Hospital of Münster; Alemania
Fil: Oetermann, Sylvia. University Hospital of Münster; Alemania
Fil: Peters, Georg. University Hospital of Münster; Alemania
Fil: Löffler, Bettina. University Hospital of Münster; Alemania. Jena University Hospital; Alemania
Fil: Tuchscherr, Lorena Paola Nelly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University Hospital of Münster; Alemania. Jena University Hospital; Alemania
description Osteomyelitis is a severe inflammatory disease of the bone that is mainly caused by Staphylococcus aureus. Particularly, bone infections are difficult to treat and can develop into a chronic course with a high relapsing rate despite of antimicrobial treatments. The complex interaction of staphylococci with osseous tissue and the bacterial ability to invade host cells are thought to determine the severity of infection. Yet, defined bacterial virulence factors responsible for the pathogenesis of osteomyelitis have not been clearly identified. The aim of this study was to detect S. aureus virulence factors that are associated with osteomyelitis and contribute to a chronic course of infection. To this purpose, we collected 41 S. aureus isolates, each 11 from acute osteomyelitis (infection period less than 2 months), 10 from chronic osteomyelitis (infection period more than 12 months), 10 from sepsis and 10 from nasal colonization. All isolates were analyzed for gene expression and in functional in-vitro systems. Adhesion assays to bone matrix revealed that all isolates equally bound to matrix structures, but invasion assays in human osteoblasts showed a high invasive capacity of chronic osteomyelitis isolates. The high invasion rate could not be explained by defined adhesins, as all infecting strains expressed a multitude of adhesins that act together and determine the level of adhesion. Following host cell invasion isolates from chronic osteomyelitis induced less cytotoxicity than all other isolates and a higher percentage of Small-colony-variant (SCV)-formation, which represents an adaptation mechanism during long-term persistence. Isolates from acute and chronic osteomyelitis strongly produced biofilm and highly expressed agr and sarA that regulate secreted virulence factors and induced an inflammatory response in osteoblasts. In conclusion, chronic osteomyelitis isolates were characterized by a high host cell invasion rate, low cytotoxicity and the ability to persist and adapt within osteoblasts. Furthermore, isolates from both acute and chronic osteomyelitis strongly produced biofilm and induced high levels of host cell inflammation, which may explain tissue destruction and bone deformation observed as typical complications of long-lasting bone infections.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94483
Kalinka, Julia; Hachmeister, Marie; Geraci, Jennifer; Sordelli, Daniel Oscar; Hansen, Uwe; et al.; Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation; Elsevier Gmbh; International Journal of Medical Microbiology (print); 304; 8; 11-2014; 1038-1049
1438-4221
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94483
identifier_str_mv Kalinka, Julia; Hachmeister, Marie; Geraci, Jennifer; Sordelli, Daniel Oscar; Hansen, Uwe; et al.; Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation; Elsevier Gmbh; International Journal of Medical Microbiology (print); 304; 8; 11-2014; 1038-1049
1438-4221
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1438422114000952
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijmm.2014.07.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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