Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode

Autores
Aloisio, Carolina; Razola Díaz, María del Carmen; Aznar Ramos, María José; Longhi, Marcela R.; Andreatta, Alfonsina Ester; Verardo, Vito
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
Fil: Aloisio, Carolina. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Razola Díaz, María del Carmen. Universidad de Granada; España
Fil: Aznar Ramos, María José. Universidad de Granada; España
Fil: Longhi, Marcela R.. Universidad Nacional de Córdoba; Argentina
Fil: Andreatta, Alfonsina Ester. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Verardo, Vito. Universidad de Granada; España
Materia
BOX-BEHNKEN DESIGN
HPLC-ESI-TOF-MS
SWEET BASIL (OCIMUM BASILICUM)
ULTRASONIC-ASSISTED EXTRACTION VIA A SONOTRODE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222398
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/222398
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a SonotrodeAloisio, CarolinaRazola Díaz, María del CarmenAznar Ramos, María JoséLonghi, Marcela R.Andreatta, Alfonsina EsterVerardo, VitoBOX-BEHNKEN DESIGNHPLC-ESI-TOF-MSSWEET BASIL (OCIMUM BASILICUM)ULTRASONIC-ASSISTED EXTRACTION VIA A SONOTRODEhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.Fil: Aloisio, Carolina. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Razola Díaz, María del Carmen. Universidad de Granada; EspañaFil: Aznar Ramos, María José. Universidad de Granada; EspañaFil: Longhi, Marcela R.. Universidad Nacional de Córdoba; ArgentinaFil: Andreatta, Alfonsina Ester. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Verardo, Vito. Universidad de Granada; EspañaMolecular Diversity Preservation International2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222398Aloisio, Carolina; Razola Díaz, María del Carmen; Aznar Ramos, María José; Longhi, Marcela R.; Andreatta, Alfonsina Ester; et al.; Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode; Molecular Diversity Preservation International; Molecules; 28; 13; 7-2023; 1-141420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/molecules28135286info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:52Zoai:ri.conicet.gov.ar:11336/222398instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:53.091CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
title Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
spellingShingle Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
Aloisio, Carolina
BOX-BEHNKEN DESIGN
HPLC-ESI-TOF-MS
SWEET BASIL (OCIMUM BASILICUM)
ULTRASONIC-ASSISTED EXTRACTION VIA A SONOTRODE
title_short Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
title_full Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
title_fullStr Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
title_full_unstemmed Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
title_sort Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
dc.creator.none.fl_str_mv Aloisio, Carolina
Razola Díaz, María del Carmen
Aznar Ramos, María José
Longhi, Marcela R.
Andreatta, Alfonsina Ester
Verardo, Vito
author Aloisio, Carolina
author_facet Aloisio, Carolina
Razola Díaz, María del Carmen
Aznar Ramos, María José
Longhi, Marcela R.
Andreatta, Alfonsina Ester
Verardo, Vito
author_role author
author2 Razola Díaz, María del Carmen
Aznar Ramos, María José
Longhi, Marcela R.
Andreatta, Alfonsina Ester
Verardo, Vito
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BOX-BEHNKEN DESIGN
HPLC-ESI-TOF-MS
SWEET BASIL (OCIMUM BASILICUM)
ULTRASONIC-ASSISTED EXTRACTION VIA A SONOTRODE
topic BOX-BEHNKEN DESIGN
HPLC-ESI-TOF-MS
SWEET BASIL (OCIMUM BASILICUM)
ULTRASONIC-ASSISTED EXTRACTION VIA A SONOTRODE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
Fil: Aloisio, Carolina. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Razola Díaz, María del Carmen. Universidad de Granada; España
Fil: Aznar Ramos, María José. Universidad de Granada; España
Fil: Longhi, Marcela R.. Universidad Nacional de Córdoba; Argentina
Fil: Andreatta, Alfonsina Ester. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Verardo, Vito. Universidad de Granada; España
description Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222398
Aloisio, Carolina; Razola Díaz, María del Carmen; Aznar Ramos, María José; Longhi, Marcela R.; Andreatta, Alfonsina Ester; et al.; Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode; Molecular Diversity Preservation International; Molecules; 28; 13; 7-2023; 1-14
1420-3049
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222398
identifier_str_mv Aloisio, Carolina; Razola Díaz, María del Carmen; Aznar Ramos, María José; Longhi, Marcela R.; Andreatta, Alfonsina Ester; et al.; Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode; Molecular Diversity Preservation International; Molecules; 28; 13; 7-2023; 1-14
1420-3049
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules28135286
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269932555862016
score 13.13397

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