Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
- Autores
- Bergero, Maria Fernanda; Lucchesi, Gloria Ines
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Pseudomonas putida A (ATCC 12633) were encapsulated in Ca-alginate and their ability to degrade the quaternary ammonium compounds (QACs) tetradecylbenzyldimethylammonium chloride (C14BDMA), hexadecylbenzyldimethylammonium chloride (C16BDMA) and benzalkonium chloride (BAC) (a mixture 1:1 of C14BDMA and C16BDMA), was tested and compared with the degradation of the same compounds by freely suspended cells. Degradation was carried out in pH 7.4 buffered medium, at 30 °C with agitation at 100 rpm. After 24 h of incubation immobilized cells degraded 90 % of 35 to 315 mg l-1 of C14BDMA, C16BDMA and BAC, whereas free cells were unable to degrade concentrations above 35 mg l-1. Immobilized cells of P. putida removed 80 % QACs of an industrial effluent in 48 h under both sterile and non-sterile conditions. Degradation of QACs carried out using the non-sterilized effluent showed that the beads could be reused up to four cycles without changing the degradation efficiency. The reduction in the QACs concentration after treatment with immobilized cells can be correlated with the reduction in the phytotoxicity to Lactuta sativa seed germination and root elongation. We conclude that immobilized QACs-degrading bacteria represent a promising tool for bioremediation of QACs in industrial wastewater systems.
Fil: Bergero, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina - Materia
-
Biodegradation.
Benzalkonium Chloride.
Pseudomonas Putida.
Alginate - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/44895
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Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluentsBergero, Maria FernandaLucchesi, Gloria InesBiodegradation.Benzalkonium Chloride.Pseudomonas Putida.Alginatehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pseudomonas putida A (ATCC 12633) were encapsulated in Ca-alginate and their ability to degrade the quaternary ammonium compounds (QACs) tetradecylbenzyldimethylammonium chloride (C14BDMA), hexadecylbenzyldimethylammonium chloride (C16BDMA) and benzalkonium chloride (BAC) (a mixture 1:1 of C14BDMA and C16BDMA), was tested and compared with the degradation of the same compounds by freely suspended cells. Degradation was carried out in pH 7.4 buffered medium, at 30 °C with agitation at 100 rpm. After 24 h of incubation immobilized cells degraded 90 % of 35 to 315 mg l-1 of C14BDMA, C16BDMA and BAC, whereas free cells were unable to degrade concentrations above 35 mg l-1. Immobilized cells of P. putida removed 80 % QACs of an industrial effluent in 48 h under both sterile and non-sterile conditions. Degradation of QACs carried out using the non-sterilized effluent showed that the beads could be reused up to four cycles without changing the degradation efficiency. The reduction in the QACs concentration after treatment with immobilized cells can be correlated with the reduction in the phytotoxicity to Lactuta sativa seed germination and root elongation. We conclude that immobilized QACs-degrading bacteria represent a promising tool for bioremediation of QACs in industrial wastewater systems.Fil: Bergero, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaElsevier2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44895Bergero, Maria Fernanda; Lucchesi, Gloria Ines; Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents; Elsevier; International Biodeterioration and Biodegradation; 100; 2-2015; 38-430964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.02.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830515000360info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:01:14Zoai:ri.conicet.gov.ar:11336/44895instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:01:14.502CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents |
title |
Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents |
spellingShingle |
Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents Bergero, Maria Fernanda Biodegradation. Benzalkonium Chloride. Pseudomonas Putida. Alginate |
title_short |
Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents |
title_full |
Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents |
title_fullStr |
Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents |
title_full_unstemmed |
Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents |
title_sort |
Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents |
dc.creator.none.fl_str_mv |
Bergero, Maria Fernanda Lucchesi, Gloria Ines |
author |
Bergero, Maria Fernanda |
author_facet |
Bergero, Maria Fernanda Lucchesi, Gloria Ines |
author_role |
author |
author2 |
Lucchesi, Gloria Ines |
author2_role |
author |
dc.subject.none.fl_str_mv |
Biodegradation. Benzalkonium Chloride. Pseudomonas Putida. Alginate |
topic |
Biodegradation. Benzalkonium Chloride. Pseudomonas Putida. Alginate |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Pseudomonas putida A (ATCC 12633) were encapsulated in Ca-alginate and their ability to degrade the quaternary ammonium compounds (QACs) tetradecylbenzyldimethylammonium chloride (C14BDMA), hexadecylbenzyldimethylammonium chloride (C16BDMA) and benzalkonium chloride (BAC) (a mixture 1:1 of C14BDMA and C16BDMA), was tested and compared with the degradation of the same compounds by freely suspended cells. Degradation was carried out in pH 7.4 buffered medium, at 30 °C with agitation at 100 rpm. After 24 h of incubation immobilized cells degraded 90 % of 35 to 315 mg l-1 of C14BDMA, C16BDMA and BAC, whereas free cells were unable to degrade concentrations above 35 mg l-1. Immobilized cells of P. putida removed 80 % QACs of an industrial effluent in 48 h under both sterile and non-sterile conditions. Degradation of QACs carried out using the non-sterilized effluent showed that the beads could be reused up to four cycles without changing the degradation efficiency. The reduction in the QACs concentration after treatment with immobilized cells can be correlated with the reduction in the phytotoxicity to Lactuta sativa seed germination and root elongation. We conclude that immobilized QACs-degrading bacteria represent a promising tool for bioremediation of QACs in industrial wastewater systems. Fil: Bergero, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina Fil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina |
description |
Pseudomonas putida A (ATCC 12633) were encapsulated in Ca-alginate and their ability to degrade the quaternary ammonium compounds (QACs) tetradecylbenzyldimethylammonium chloride (C14BDMA), hexadecylbenzyldimethylammonium chloride (C16BDMA) and benzalkonium chloride (BAC) (a mixture 1:1 of C14BDMA and C16BDMA), was tested and compared with the degradation of the same compounds by freely suspended cells. Degradation was carried out in pH 7.4 buffered medium, at 30 °C with agitation at 100 rpm. After 24 h of incubation immobilized cells degraded 90 % of 35 to 315 mg l-1 of C14BDMA, C16BDMA and BAC, whereas free cells were unable to degrade concentrations above 35 mg l-1. Immobilized cells of P. putida removed 80 % QACs of an industrial effluent in 48 h under both sterile and non-sterile conditions. Degradation of QACs carried out using the non-sterilized effluent showed that the beads could be reused up to four cycles without changing the degradation efficiency. The reduction in the QACs concentration after treatment with immobilized cells can be correlated with the reduction in the phytotoxicity to Lactuta sativa seed germination and root elongation. We conclude that immobilized QACs-degrading bacteria represent a promising tool for bioremediation of QACs in industrial wastewater systems. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/44895 Bergero, Maria Fernanda; Lucchesi, Gloria Ines; Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents; Elsevier; International Biodeterioration and Biodegradation; 100; 2-2015; 38-43 0964-8305 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/44895 |
identifier_str_mv |
Bergero, Maria Fernanda; Lucchesi, Gloria Ines; Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents; Elsevier; International Biodeterioration and Biodegradation; 100; 2-2015; 38-43 0964-8305 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.02.004 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830515000360 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846781192243773440 |
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12.982451 |