Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents

Autores
Bergero, Maria Fernanda; Lucchesi, Gloria Ines
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas putida A (ATCC 12633) were encapsulated in Ca-alginate and their ability to degrade the quaternary ammonium compounds (QACs) tetradecylbenzyldimethylammonium chloride (C14BDMA), hexadecylbenzyldimethylammonium chloride (C16BDMA) and benzalkonium chloride (BAC) (a mixture 1:1 of C14BDMA and C16BDMA), was tested and compared with the degradation of the same compounds by freely suspended cells. Degradation was carried out in pH 7.4 buffered medium, at 30 °C with agitation at 100 rpm. After 24 h of incubation immobilized cells degraded 90 % of 35 to 315 mg l-1 of C14BDMA, C16BDMA and BAC, whereas free cells were unable to degrade concentrations above 35 mg l-1. Immobilized cells of P. putida removed 80 % QACs of an industrial effluent in 48 h under both sterile and non-sterile conditions. Degradation of QACs carried out using the non-sterilized effluent showed that the beads could be reused up to four cycles without changing the degradation efficiency. The reduction in the QACs concentration after treatment with immobilized cells can be correlated with the reduction in the phytotoxicity to Lactuta sativa seed germination and root elongation. We conclude that immobilized QACs-degrading bacteria represent a promising tool for bioremediation of QACs in industrial wastewater systems.
Fil: Bergero, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Materia
Biodegradation.
Benzalkonium Chloride.
Pseudomonas Putida.
Alginate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44895
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44895
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluentsBergero, Maria FernandaLucchesi, Gloria InesBiodegradation.Benzalkonium Chloride.Pseudomonas Putida.Alginatehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pseudomonas putida A (ATCC 12633) were encapsulated in Ca-alginate and their ability to degrade the quaternary ammonium compounds (QACs) tetradecylbenzyldimethylammonium chloride (C14BDMA), hexadecylbenzyldimethylammonium chloride (C16BDMA) and benzalkonium chloride (BAC) (a mixture 1:1 of C14BDMA and C16BDMA), was tested and compared with the degradation of the same compounds by freely suspended cells. Degradation was carried out in pH 7.4 buffered medium, at 30 °C with agitation at 100 rpm. After 24 h of incubation immobilized cells degraded 90 % of 35 to 315 mg l-1 of C14BDMA, C16BDMA and BAC, whereas free cells were unable to degrade concentrations above 35 mg l-1. Immobilized cells of P. putida removed 80 % QACs of an industrial effluent in 48 h under both sterile and non-sterile conditions. Degradation of QACs carried out using the non-sterilized effluent showed that the beads could be reused up to four cycles without changing the degradation efficiency. The reduction in the QACs concentration after treatment with immobilized cells can be correlated with the reduction in the phytotoxicity to Lactuta sativa seed germination and root elongation. We conclude that immobilized QACs-degrading bacteria represent a promising tool for bioremediation of QACs in industrial wastewater systems.Fil: Bergero, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaElsevier2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44895Bergero, Maria Fernanda; Lucchesi, Gloria Ines; Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents; Elsevier; International Biodeterioration and Biodegradation; 100; 2-2015; 38-430964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.02.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830515000360info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:01:14Zoai:ri.conicet.gov.ar:11336/44895instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:01:14.502CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
title Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
spellingShingle Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
Bergero, Maria Fernanda
Biodegradation.
Benzalkonium Chloride.
Pseudomonas Putida.
Alginate
title_short Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
title_full Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
title_fullStr Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
title_full_unstemmed Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
title_sort Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents
dc.creator.none.fl_str_mv Bergero, Maria Fernanda
Lucchesi, Gloria Ines
author Bergero, Maria Fernanda
author_facet Bergero, Maria Fernanda
Lucchesi, Gloria Ines
author_role author
author2 Lucchesi, Gloria Ines
author2_role author
dc.subject.none.fl_str_mv Biodegradation.
Benzalkonium Chloride.
Pseudomonas Putida.
Alginate
topic Biodegradation.
Benzalkonium Chloride.
Pseudomonas Putida.
Alginate
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pseudomonas putida A (ATCC 12633) were encapsulated in Ca-alginate and their ability to degrade the quaternary ammonium compounds (QACs) tetradecylbenzyldimethylammonium chloride (C14BDMA), hexadecylbenzyldimethylammonium chloride (C16BDMA) and benzalkonium chloride (BAC) (a mixture 1:1 of C14BDMA and C16BDMA), was tested and compared with the degradation of the same compounds by freely suspended cells. Degradation was carried out in pH 7.4 buffered medium, at 30 °C with agitation at 100 rpm. After 24 h of incubation immobilized cells degraded 90 % of 35 to 315 mg l-1 of C14BDMA, C16BDMA and BAC, whereas free cells were unable to degrade concentrations above 35 mg l-1. Immobilized cells of P. putida removed 80 % QACs of an industrial effluent in 48 h under both sterile and non-sterile conditions. Degradation of QACs carried out using the non-sterilized effluent showed that the beads could be reused up to four cycles without changing the degradation efficiency. The reduction in the QACs concentration after treatment with immobilized cells can be correlated with the reduction in the phytotoxicity to Lactuta sativa seed germination and root elongation. We conclude that immobilized QACs-degrading bacteria represent a promising tool for bioremediation of QACs in industrial wastewater systems.
Fil: Bergero, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
description Pseudomonas putida A (ATCC 12633) were encapsulated in Ca-alginate and their ability to degrade the quaternary ammonium compounds (QACs) tetradecylbenzyldimethylammonium chloride (C14BDMA), hexadecylbenzyldimethylammonium chloride (C16BDMA) and benzalkonium chloride (BAC) (a mixture 1:1 of C14BDMA and C16BDMA), was tested and compared with the degradation of the same compounds by freely suspended cells. Degradation was carried out in pH 7.4 buffered medium, at 30 °C with agitation at 100 rpm. After 24 h of incubation immobilized cells degraded 90 % of 35 to 315 mg l-1 of C14BDMA, C16BDMA and BAC, whereas free cells were unable to degrade concentrations above 35 mg l-1. Immobilized cells of P. putida removed 80 % QACs of an industrial effluent in 48 h under both sterile and non-sterile conditions. Degradation of QACs carried out using the non-sterilized effluent showed that the beads could be reused up to four cycles without changing the degradation efficiency. The reduction in the QACs concentration after treatment with immobilized cells can be correlated with the reduction in the phytotoxicity to Lactuta sativa seed germination and root elongation. We conclude that immobilized QACs-degrading bacteria represent a promising tool for bioremediation of QACs in industrial wastewater systems.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44895
Bergero, Maria Fernanda; Lucchesi, Gloria Ines; Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents; Elsevier; International Biodeterioration and Biodegradation; 100; 2-2015; 38-43
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44895
identifier_str_mv Bergero, Maria Fernanda; Lucchesi, Gloria Ines; Immobilization of Pseudomonas putida A (ATCC 12633) cells: A promising tool for effective degradation of quaternary ammonium compounds in industrial effluents; Elsevier; International Biodeterioration and Biodegradation; 100; 2-2015; 38-43
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.02.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830515000360
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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