Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus...

Autores
Vrdoljak, Juan Esteban; Sánchez, Kevin Imanol; Arreola Ramos, Roberto; Diaz Huesa, Emilce Guadalupe; Villagra, Alejandro; Avila, Luciano Javier; Morando, Mariana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The repeatability of findings is the key factor behind scientific reliability, and the failure to reproduce scientificfindings has been termed the "replication crisis". Geometric morphometrics is an established tool in evolutionarybiology. However, different operators (and/or different methods) could act as large sources of variation in the dataobtained. Here, we investigated inter-operator error in geometric morphometric protocols on complex shapes ofLiolaemus lizards, as well as measurement error in three taxa varying in their difficulty of digitalization. We alsoexamined the potential for these protocols to discriminate among complex shapes in closely related species. Wefound a wide range of inter-operator error, contributing between 19.5% and 60% to the total variation. Moreover,measurement error increased with the complexity of the quantified shape. All protocols were able to discriminatebetween species, but the use of more landmarks did not imply better performance. We present evidence that complexshapes reduce repeatability, highlighting the need to explore different sources of variation that could lead to suchlow repeatability. Lastly, we suggest some recommendations to improve the repeatability and reliability of geometricmorphometrics results.
Fil: Vrdoljak, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Sánchez, Kevin Imanol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Arreola Ramos, Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Diaz Huesa, Emilce Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina
Fil: Villagra, Alejandro. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Avila, Luciano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Morando, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Materia
COMPLEX SHAPES
GEOMETRIC MORPHOMETRICS
INTER-OPERATOR ERROR
MEASUREMENT ERROR
REPLICATION CRISIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/135182
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/135182
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)Vrdoljak, Juan EstebanSánchez, Kevin ImanolArreola Ramos, RobertoDiaz Huesa, Emilce GuadalupeVillagra, AlejandroAvila, Luciano JavierMorando, MarianaCOMPLEX SHAPESGEOMETRIC MORPHOMETRICSINTER-OPERATOR ERRORMEASUREMENT ERRORREPLICATION CRISIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The repeatability of findings is the key factor behind scientific reliability, and the failure to reproduce scientificfindings has been termed the "replication crisis". Geometric morphometrics is an established tool in evolutionarybiology. However, different operators (and/or different methods) could act as large sources of variation in the dataobtained. Here, we investigated inter-operator error in geometric morphometric protocols on complex shapes ofLiolaemus lizards, as well as measurement error in three taxa varying in their difficulty of digitalization. We alsoexamined the potential for these protocols to discriminate among complex shapes in closely related species. Wefound a wide range of inter-operator error, contributing between 19.5% and 60% to the total variation. Moreover,measurement error increased with the complexity of the quantified shape. All protocols were able to discriminatebetween species, but the use of more landmarks did not imply better performance. We present evidence that complexshapes reduce repeatability, highlighting the need to explore different sources of variation that could lead to suchlow repeatability. Lastly, we suggest some recommendations to improve the repeatability and reliability of geometricmorphometrics results.Fil: Vrdoljak, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Sánchez, Kevin Imanol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Arreola Ramos, Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Diaz Huesa, Emilce Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Villagra, Alejandro. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Avila, Luciano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Morando, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaWiley Blackwell Publishing, Inc2020-07-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/135182Vrdoljak, Juan Esteban; Sánchez, Kevin Imanol; Arreola Ramos, Roberto; Diaz Huesa, Emilce Guadalupe; Villagra, Alejandro; et al.; Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini); Wiley Blackwell Publishing, Inc; Biological Journal of The Linnean Society; 130; 4; 14-7-2020; 800-8120024-40661095-8312CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolinnean/advance-article/doi/10.1093/biolinnean/blaa079/5871286info:eu-repo/semantics/altIdentifier/doi/10.1093/biolinnean/blaa079info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-04T10:56:05Zoai:ri.conicet.gov.ar:11336/135182instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-04 10:56:05.769CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
title Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
spellingShingle Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
Vrdoljak, Juan Esteban
COMPLEX SHAPES
GEOMETRIC MORPHOMETRICS
INTER-OPERATOR ERROR
MEASUREMENT ERROR
REPLICATION CRISIS
title_short Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
title_full Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
title_fullStr Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
title_full_unstemmed Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
title_sort Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
dc.creator.none.fl_str_mv Vrdoljak, Juan Esteban
Sánchez, Kevin Imanol
Arreola Ramos, Roberto
Diaz Huesa, Emilce Guadalupe
Villagra, Alejandro
Avila, Luciano Javier
Morando, Mariana
author Vrdoljak, Juan Esteban
author_facet Vrdoljak, Juan Esteban
Sánchez, Kevin Imanol
Arreola Ramos, Roberto
Diaz Huesa, Emilce Guadalupe
Villagra, Alejandro
Avila, Luciano Javier
Morando, Mariana
author_role author
author2 Sánchez, Kevin Imanol
Arreola Ramos, Roberto
Diaz Huesa, Emilce Guadalupe
Villagra, Alejandro
Avila, Luciano Javier
Morando, Mariana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv COMPLEX SHAPES
GEOMETRIC MORPHOMETRICS
INTER-OPERATOR ERROR
MEASUREMENT ERROR
REPLICATION CRISIS
topic COMPLEX SHAPES
GEOMETRIC MORPHOMETRICS
INTER-OPERATOR ERROR
MEASUREMENT ERROR
REPLICATION CRISIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The repeatability of findings is the key factor behind scientific reliability, and the failure to reproduce scientificfindings has been termed the "replication crisis". Geometric morphometrics is an established tool in evolutionarybiology. However, different operators (and/or different methods) could act as large sources of variation in the dataobtained. Here, we investigated inter-operator error in geometric morphometric protocols on complex shapes ofLiolaemus lizards, as well as measurement error in three taxa varying in their difficulty of digitalization. We alsoexamined the potential for these protocols to discriminate among complex shapes in closely related species. Wefound a wide range of inter-operator error, contributing between 19.5% and 60% to the total variation. Moreover,measurement error increased with the complexity of the quantified shape. All protocols were able to discriminatebetween species, but the use of more landmarks did not imply better performance. We present evidence that complexshapes reduce repeatability, highlighting the need to explore different sources of variation that could lead to suchlow repeatability. Lastly, we suggest some recommendations to improve the repeatability and reliability of geometricmorphometrics results.
Fil: Vrdoljak, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Sánchez, Kevin Imanol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Arreola Ramos, Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Diaz Huesa, Emilce Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina
Fil: Villagra, Alejandro. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Avila, Luciano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Morando, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
description The repeatability of findings is the key factor behind scientific reliability, and the failure to reproduce scientificfindings has been termed the "replication crisis". Geometric morphometrics is an established tool in evolutionarybiology. However, different operators (and/or different methods) could act as large sources of variation in the dataobtained. Here, we investigated inter-operator error in geometric morphometric protocols on complex shapes ofLiolaemus lizards, as well as measurement error in three taxa varying in their difficulty of digitalization. We alsoexamined the potential for these protocols to discriminate among complex shapes in closely related species. Wefound a wide range of inter-operator error, contributing between 19.5% and 60% to the total variation. Moreover,measurement error increased with the complexity of the quantified shape. All protocols were able to discriminatebetween species, but the use of more landmarks did not imply better performance. We present evidence that complexshapes reduce repeatability, highlighting the need to explore different sources of variation that could lead to suchlow repeatability. Lastly, we suggest some recommendations to improve the repeatability and reliability of geometricmorphometrics results.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/135182
Vrdoljak, Juan Esteban; Sánchez, Kevin Imanol; Arreola Ramos, Roberto; Diaz Huesa, Emilce Guadalupe; Villagra, Alejandro; et al.; Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini); Wiley Blackwell Publishing, Inc; Biological Journal of The Linnean Society; 130; 4; 14-7-2020; 800-812
0024-4066
1095-8312
CONICET Digital
CONICET
url http://hdl.handle.net/11336/135182
identifier_str_mv Vrdoljak, Juan Esteban; Sánchez, Kevin Imanol; Arreola Ramos, Roberto; Diaz Huesa, Emilce Guadalupe; Villagra, Alejandro; et al.; Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: a case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini); Wiley Blackwell Publishing, Inc; Biological Journal of The Linnean Society; 130; 4; 14-7-2020; 800-812
0024-4066
1095-8312
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolinnean/advance-article/doi/10.1093/biolinnean/blaa079/5871286
info:eu-repo/semantics/altIdentifier/doi/10.1093/biolinnean/blaa079
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.832306

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