Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains

Autores
Montaner, Alejandro Daniel; Mongiat, Lucas Alberto; Lux, Victoria Adela R.; Park, M. K.; Fischer, W. H.; Rivier, J. E.; Lescheid, D.; Lovejoy, D.; Libertun, Carlos; Sherwood, N. M.; Somoza, Gustavo Manuel
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rat and hamster brain tissues were used to investigate the possible existence of a follicle stimulating hormone (FSH)-releasing factor with similar characteristics to the lamprey gonadotropin-releasing hormone III (lGnRH-III) form proposed in previous reports. The present studies involved isolation and purification of the molecule by high-performance liquid chromatography (HPLC), identification by radioimmunoassay, sequence analysis by automated Edman degradation, mass spectrometry and examination of biological activity. Hypothalamic extracts from both species contained an HPLC fraction that was immunoreactive to GnRH and coeluted with lGnRH-III and 9-hydroxyproline mGnRH ([Hyp(9)]GnRH). Determination of primary structure from purified total brain material demonstrated that the isolated molecule was [Hyp(9)]GnRH. This is the first report showing the presence of the posttranslationally modified form already known as [Hyp(9)]GnRH by primary sequence analysis. The biological activity of distinct GnRH peptides was also tested in vitro for gonadotropin release using rat pituitary primary cell cultures. The results showed that [Hyp(9)]GnRH stimulated both luteinizing hormone and FSH release, as already reported, whereas lGnRH-III had no action on the secretion of either gonadotropin.
Fil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
Fil: Mongiat, Lucas Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Park, M. K.. The Salk Institute; Estados Unidos
Fil: Fischer, W. H.. The Salk Institute; Estados Unidos
Fil: Rivier, J. E.. The Salk Institute; Estados Unidos
Fil: Lescheid, D.. University Of Victoria; Canadá. University of Toronto; Canadá
Fil: Lovejoy, D.. University of Toronto; Canadá
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Sherwood, N. M.. University Of Victoria; Canadá. University of Toronto; Canadá
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
Materia
Gonadotropins
Processing
Structure Function Relationship
Hydroxyproline Gnrh
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31321
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31321
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brainsMontaner, Alejandro DanielMongiat, Lucas AlbertoLux, Victoria Adela R.Park, M. K.Fischer, W. H.Rivier, J. E.Lescheid, D.Lovejoy, D.Libertun, CarlosSherwood, N. M.Somoza, Gustavo ManuelGonadotropinsProcessingStructure Function RelationshipHydroxyproline Gnrhhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Rat and hamster brain tissues were used to investigate the possible existence of a follicle stimulating hormone (FSH)-releasing factor with similar characteristics to the lamprey gonadotropin-releasing hormone III (lGnRH-III) form proposed in previous reports. The present studies involved isolation and purification of the molecule by high-performance liquid chromatography (HPLC), identification by radioimmunoassay, sequence analysis by automated Edman degradation, mass spectrometry and examination of biological activity. Hypothalamic extracts from both species contained an HPLC fraction that was immunoreactive to GnRH and coeluted with lGnRH-III and 9-hydroxyproline mGnRH ([Hyp(9)]GnRH). Determination of primary structure from purified total brain material demonstrated that the isolated molecule was [Hyp(9)]GnRH. This is the first report showing the presence of the posttranslationally modified form already known as [Hyp(9)]GnRH by primary sequence analysis. The biological activity of distinct GnRH peptides was also tested in vitro for gonadotropin release using rat pituitary primary cell cultures. The results showed that [Hyp(9)]GnRH stimulated both luteinizing hormone and FSH release, as already reported, whereas lGnRH-III had no action on the secretion of either gonadotropin.Fil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; ArgentinaFil: Mongiat, Lucas Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Park, M. K.. The Salk Institute; Estados UnidosFil: Fischer, W. H.. The Salk Institute; Estados UnidosFil: Rivier, J. E.. The Salk Institute; Estados UnidosFil: Lescheid, D.. University Of Victoria; Canadá. University of Toronto; CanadáFil: Lovejoy, D.. University of Toronto; CanadáFil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Sherwood, N. M.. University Of Victoria; Canadá. University of Toronto; CanadáFil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; ArgentinaKarger2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31321Somoza, Gustavo Manuel; Sherwood, N. M.; Libertun, Carlos; Lovejoy, D.; Lescheid, D.; Rivier, J. E.; et al.; Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains; Karger; Neuroendocrinology; 74; 3; 12-2001; 202-2120028-38351423-0194CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/54687info:eu-repo/semantics/altIdentifier/doi/10.1159/000054687info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:51Zoai:ri.conicet.gov.ar:11336/31321instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:52.216CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains
title Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains
spellingShingle Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains
Montaner, Alejandro Daniel
Gonadotropins
Processing
Structure Function Relationship
Hydroxyproline Gnrh
title_short Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains
title_full Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains
title_fullStr Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains
title_full_unstemmed Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains
title_sort Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains
dc.creator.none.fl_str_mv Montaner, Alejandro Daniel
Mongiat, Lucas Alberto
Lux, Victoria Adela R.
Park, M. K.
Fischer, W. H.
Rivier, J. E.
Lescheid, D.
Lovejoy, D.
Libertun, Carlos
Sherwood, N. M.
Somoza, Gustavo Manuel
author Montaner, Alejandro Daniel
author_facet Montaner, Alejandro Daniel
Mongiat, Lucas Alberto
Lux, Victoria Adela R.
Park, M. K.
Fischer, W. H.
Rivier, J. E.
Lescheid, D.
Lovejoy, D.
Libertun, Carlos
Sherwood, N. M.
Somoza, Gustavo Manuel
author_role author
author2 Mongiat, Lucas Alberto
Lux, Victoria Adela R.
Park, M. K.
Fischer, W. H.
Rivier, J. E.
Lescheid, D.
Lovejoy, D.
Libertun, Carlos
Sherwood, N. M.
Somoza, Gustavo Manuel
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gonadotropins
Processing
Structure Function Relationship
Hydroxyproline Gnrh
topic Gonadotropins
Processing
Structure Function Relationship
Hydroxyproline Gnrh
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Rat and hamster brain tissues were used to investigate the possible existence of a follicle stimulating hormone (FSH)-releasing factor with similar characteristics to the lamprey gonadotropin-releasing hormone III (lGnRH-III) form proposed in previous reports. The present studies involved isolation and purification of the molecule by high-performance liquid chromatography (HPLC), identification by radioimmunoassay, sequence analysis by automated Edman degradation, mass spectrometry and examination of biological activity. Hypothalamic extracts from both species contained an HPLC fraction that was immunoreactive to GnRH and coeluted with lGnRH-III and 9-hydroxyproline mGnRH ([Hyp(9)]GnRH). Determination of primary structure from purified total brain material demonstrated that the isolated molecule was [Hyp(9)]GnRH. This is the first report showing the presence of the posttranslationally modified form already known as [Hyp(9)]GnRH by primary sequence analysis. The biological activity of distinct GnRH peptides was also tested in vitro for gonadotropin release using rat pituitary primary cell cultures. The results showed that [Hyp(9)]GnRH stimulated both luteinizing hormone and FSH release, as already reported, whereas lGnRH-III had no action on the secretion of either gonadotropin.
Fil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
Fil: Mongiat, Lucas Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Park, M. K.. The Salk Institute; Estados Unidos
Fil: Fischer, W. H.. The Salk Institute; Estados Unidos
Fil: Rivier, J. E.. The Salk Institute; Estados Unidos
Fil: Lescheid, D.. University Of Victoria; Canadá. University of Toronto; Canadá
Fil: Lovejoy, D.. University of Toronto; Canadá
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Sherwood, N. M.. University Of Victoria; Canadá. University of Toronto; Canadá
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
description Rat and hamster brain tissues were used to investigate the possible existence of a follicle stimulating hormone (FSH)-releasing factor with similar characteristics to the lamprey gonadotropin-releasing hormone III (lGnRH-III) form proposed in previous reports. The present studies involved isolation and purification of the molecule by high-performance liquid chromatography (HPLC), identification by radioimmunoassay, sequence analysis by automated Edman degradation, mass spectrometry and examination of biological activity. Hypothalamic extracts from both species contained an HPLC fraction that was immunoreactive to GnRH and coeluted with lGnRH-III and 9-hydroxyproline mGnRH ([Hyp(9)]GnRH). Determination of primary structure from purified total brain material demonstrated that the isolated molecule was [Hyp(9)]GnRH. This is the first report showing the presence of the posttranslationally modified form already known as [Hyp(9)]GnRH by primary sequence analysis. The biological activity of distinct GnRH peptides was also tested in vitro for gonadotropin release using rat pituitary primary cell cultures. The results showed that [Hyp(9)]GnRH stimulated both luteinizing hormone and FSH release, as already reported, whereas lGnRH-III had no action on the secretion of either gonadotropin.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31321
Somoza, Gustavo Manuel; Sherwood, N. M.; Libertun, Carlos; Lovejoy, D.; Lescheid, D.; Rivier, J. E.; et al.; Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains; Karger; Neuroendocrinology; 74; 3; 12-2001; 202-212
0028-3835
1423-0194
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31321
identifier_str_mv Somoza, Gustavo Manuel; Sherwood, N. M.; Libertun, Carlos; Lovejoy, D.; Lescheid, D.; Rivier, J. E.; et al.; Structure and biological activity of gonadotropin-releasing hormone isoforms isolated from rat and hamster brains; Karger; Neuroendocrinology; 74; 3; 12-2001; 202-212
0028-3835
1423-0194
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/54687
info:eu-repo/semantics/altIdentifier/doi/10.1159/000054687
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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