Effects of irradiated biodegradable polymer in endothelial cell monolayer formation

Autores
Arbeitman, Claudia Roxana; del Grosso, Mariela Fernanda; Behar, Moni; Garcia Bermudez, Gerardo Jose
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we study cell adhesion, proliferation and cell morphology of endothelial cell cultured on poly-l-lactide acid (PLLA) modified by heavy ion irradiation. Thin films of PLLA samples were irradiated with sulfur (S) at energies of 75 MeV and gold (Au) at 18 MeV ion-beams. Ion beams were provided by the Tandar (Buenos Aires, Argentina) and Tandetron (Porto Alegre, Brazil) accelerators, respectively. The growth of a monolayer of bovine aortic endothelial cells (BAEC) onto unirradiated and irradiated surfaces has been studied by in vitro techniques in static culture. Cell viability and proliferation increased on modified substrates. But the results on unirradiated samples, indicate cell death (necrosis/apoptosis) with the consequent decrease in proliferation. We analyzed the correlation between irradiation parameters and cell metabolism and morphology.
Fil: Arbeitman, Claudia Roxana. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: del Grosso, Mariela Fernanda. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Behar, Moni. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Garcia Bermudez, Gerardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Materia
Plla
Irradiated Polymers
Swift Heavy Ions
Cell Proliferation
Cell Adhesion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24900
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24900
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of irradiated biodegradable polymer in endothelial cell monolayer formationArbeitman, Claudia Roxanadel Grosso, Mariela FernandaBehar, MoniGarcia Bermudez, Gerardo JosePllaIrradiated PolymersSwift Heavy IonsCell ProliferationCell Adhesionhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2In this work we study cell adhesion, proliferation and cell morphology of endothelial cell cultured on poly-l-lactide acid (PLLA) modified by heavy ion irradiation. Thin films of PLLA samples were irradiated with sulfur (S) at energies of 75 MeV and gold (Au) at 18 MeV ion-beams. Ion beams were provided by the Tandar (Buenos Aires, Argentina) and Tandetron (Porto Alegre, Brazil) accelerators, respectively. The growth of a monolayer of bovine aortic endothelial cells (BAEC) onto unirradiated and irradiated surfaces has been studied by in vitro techniques in static culture. Cell viability and proliferation increased on modified substrates. But the results on unirradiated samples, indicate cell death (necrosis/apoptosis) with the consequent decrease in proliferation. We analyzed the correlation between irradiation parameters and cell metabolism and morphology.Fil: Arbeitman, Claudia Roxana. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: del Grosso, Mariela Fernanda. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Behar, Moni. Universidade Federal do Rio Grande do Sul; BrasilFil: Garcia Bermudez, Gerardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaElsevier2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24900Arbeitman, Claudia Roxana; del Grosso, Mariela Fernanda; Behar, Moni; Garcia Bermudez, Gerardo Jose; Effects of irradiated biodegradable polymer in endothelial cell monolayer formation; Elsevier; Nuclear Instruments and Methods in Physics Research B: Beam Interactions with Materials and Atoms; 314; 6-2013; 86-890168-583XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.nimb.2013.05.039info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:37Zoai:ri.conicet.gov.ar:11336/24900instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:38.276CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of irradiated biodegradable polymer in endothelial cell monolayer formation
title Effects of irradiated biodegradable polymer in endothelial cell monolayer formation
spellingShingle Effects of irradiated biodegradable polymer in endothelial cell monolayer formation
Arbeitman, Claudia Roxana
Plla
Irradiated Polymers
Swift Heavy Ions
Cell Proliferation
Cell Adhesion
title_short Effects of irradiated biodegradable polymer in endothelial cell monolayer formation
title_full Effects of irradiated biodegradable polymer in endothelial cell monolayer formation
title_fullStr Effects of irradiated biodegradable polymer in endothelial cell monolayer formation
title_full_unstemmed Effects of irradiated biodegradable polymer in endothelial cell monolayer formation
title_sort Effects of irradiated biodegradable polymer in endothelial cell monolayer formation
dc.creator.none.fl_str_mv Arbeitman, Claudia Roxana
del Grosso, Mariela Fernanda
Behar, Moni
Garcia Bermudez, Gerardo Jose
author Arbeitman, Claudia Roxana
author_facet Arbeitman, Claudia Roxana
del Grosso, Mariela Fernanda
Behar, Moni
Garcia Bermudez, Gerardo Jose
author_role author
author2 del Grosso, Mariela Fernanda
Behar, Moni
Garcia Bermudez, Gerardo Jose
author2_role author
author
author
dc.subject.none.fl_str_mv Plla
Irradiated Polymers
Swift Heavy Ions
Cell Proliferation
Cell Adhesion
topic Plla
Irradiated Polymers
Swift Heavy Ions
Cell Proliferation
Cell Adhesion
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this work we study cell adhesion, proliferation and cell morphology of endothelial cell cultured on poly-l-lactide acid (PLLA) modified by heavy ion irradiation. Thin films of PLLA samples were irradiated with sulfur (S) at energies of 75 MeV and gold (Au) at 18 MeV ion-beams. Ion beams were provided by the Tandar (Buenos Aires, Argentina) and Tandetron (Porto Alegre, Brazil) accelerators, respectively. The growth of a monolayer of bovine aortic endothelial cells (BAEC) onto unirradiated and irradiated surfaces has been studied by in vitro techniques in static culture. Cell viability and proliferation increased on modified substrates. But the results on unirradiated samples, indicate cell death (necrosis/apoptosis) with the consequent decrease in proliferation. We analyzed the correlation between irradiation parameters and cell metabolism and morphology.
Fil: Arbeitman, Claudia Roxana. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: del Grosso, Mariela Fernanda. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Behar, Moni. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Garcia Bermudez, Gerardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia Física (Centro Atómico Constituyentes). Proyecto Tandar; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
description In this work we study cell adhesion, proliferation and cell morphology of endothelial cell cultured on poly-l-lactide acid (PLLA) modified by heavy ion irradiation. Thin films of PLLA samples were irradiated with sulfur (S) at energies of 75 MeV and gold (Au) at 18 MeV ion-beams. Ion beams were provided by the Tandar (Buenos Aires, Argentina) and Tandetron (Porto Alegre, Brazil) accelerators, respectively. The growth of a monolayer of bovine aortic endothelial cells (BAEC) onto unirradiated and irradiated surfaces has been studied by in vitro techniques in static culture. Cell viability and proliferation increased on modified substrates. But the results on unirradiated samples, indicate cell death (necrosis/apoptosis) with the consequent decrease in proliferation. We analyzed the correlation between irradiation parameters and cell metabolism and morphology.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24900
Arbeitman, Claudia Roxana; del Grosso, Mariela Fernanda; Behar, Moni; Garcia Bermudez, Gerardo Jose; Effects of irradiated biodegradable polymer in endothelial cell monolayer formation; Elsevier; Nuclear Instruments and Methods in Physics Research B: Beam Interactions with Materials and Atoms; 314; 6-2013; 86-89
0168-583X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24900
identifier_str_mv Arbeitman, Claudia Roxana; del Grosso, Mariela Fernanda; Behar, Moni; Garcia Bermudez, Gerardo Jose; Effects of irradiated biodegradable polymer in endothelial cell monolayer formation; Elsevier; Nuclear Instruments and Methods in Physics Research B: Beam Interactions with Materials and Atoms; 314; 6-2013; 86-89
0168-583X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nimb.2013.05.039
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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