Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study

Autores
Capdevila, Daiana Andrea; Marmisollé, Waldemar Alejandro; Williams, Federico Jose; Murgida, Daniel Horacio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study of proteins immobilized on biomimetic or biocompatible electrodes represents an active field of research as it pursues both fundamental and technological interests. In this context, adsorption and redox properties of cytochrome c (Cyt) on different electrode surfaces have been extensively reported, although in some cases with contradictory results. Here we report a SERR spectroelectrochemical study of the adsorption and electron transfer behaviour of the basic protein Cyt on electrodes coated with amino-terminated monolayers. The obtained results show that inorganic phosphate (Pi) and ATP anions are able to mediate high affinity binding of the protein with preservation of the native structure and rendering an average orientation that guarantees efficient pathways for direct electron transfer. These findings aid the design of Cyt-based bioelectronic devices and understanding the modulation by Pi and ATP of physiological functions of Cyt.
Fil: Capdevila, Daiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Williams, Federico Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Materia
Cytochrome C
Electron Transfer
Surface-Enhanced Raman
Self-Assembled Monolayers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7817
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical StudyCapdevila, Daiana AndreaMarmisollé, Waldemar AlejandroWilliams, Federico JoseMurgida, Daniel HoracioCytochrome CElectron TransferSurface-Enhanced RamanSelf-Assembled Monolayershttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The study of proteins immobilized on biomimetic or biocompatible electrodes represents an active field of research as it pursues both fundamental and technological interests. In this context, adsorption and redox properties of cytochrome c (Cyt) on different electrode surfaces have been extensively reported, although in some cases with contradictory results. Here we report a SERR spectroelectrochemical study of the adsorption and electron transfer behaviour of the basic protein Cyt on electrodes coated with amino-terminated monolayers. The obtained results show that inorganic phosphate (Pi) and ATP anions are able to mediate high affinity binding of the protein with preservation of the native structure and rendering an average orientation that guarantees efficient pathways for direct electron transfer. These findings aid the design of Cyt-based bioelectronic devices and understanding the modulation by Pi and ATP of physiological functions of Cyt.Fil: Capdevila, Daiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; ArgentinaFil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; ArgentinaFil: Williams, Federico Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; ArgentinaFil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; ArgentinaRoyal Society Of Chemistry2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7817Capdevila, Daiana Andrea; Marmisollé, Waldemar Alejandro; Williams, Federico Jose; Murgida, Daniel Horacio; Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study; Royal Society Of Chemistry; Physical Chemistry Chemical Physics; 15; 15; 3-2013; 5386-53941463-9076enginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c2cp42044ainfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2013/cp/c2cp42044a#!divAbstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:19Zoai:ri.conicet.gov.ar:11336/7817instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:19.398CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study
title Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study
spellingShingle Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study
Capdevila, Daiana Andrea
Cytochrome C
Electron Transfer
Surface-Enhanced Raman
Self-Assembled Monolayers
title_short Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study
title_full Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study
title_fullStr Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study
title_full_unstemmed Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study
title_sort Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study
dc.creator.none.fl_str_mv Capdevila, Daiana Andrea
Marmisollé, Waldemar Alejandro
Williams, Federico Jose
Murgida, Daniel Horacio
author Capdevila, Daiana Andrea
author_facet Capdevila, Daiana Andrea
Marmisollé, Waldemar Alejandro
Williams, Federico Jose
Murgida, Daniel Horacio
author_role author
author2 Marmisollé, Waldemar Alejandro
Williams, Federico Jose
Murgida, Daniel Horacio
author2_role author
author
author
dc.subject.none.fl_str_mv Cytochrome C
Electron Transfer
Surface-Enhanced Raman
Self-Assembled Monolayers
topic Cytochrome C
Electron Transfer
Surface-Enhanced Raman
Self-Assembled Monolayers
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The study of proteins immobilized on biomimetic or biocompatible electrodes represents an active field of research as it pursues both fundamental and technological interests. In this context, adsorption and redox properties of cytochrome c (Cyt) on different electrode surfaces have been extensively reported, although in some cases with contradictory results. Here we report a SERR spectroelectrochemical study of the adsorption and electron transfer behaviour of the basic protein Cyt on electrodes coated with amino-terminated monolayers. The obtained results show that inorganic phosphate (Pi) and ATP anions are able to mediate high affinity binding of the protein with preservation of the native structure and rendering an average orientation that guarantees efficient pathways for direct electron transfer. These findings aid the design of Cyt-based bioelectronic devices and understanding the modulation by Pi and ATP of physiological functions of Cyt.
Fil: Capdevila, Daiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Williams, Federico Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
description The study of proteins immobilized on biomimetic or biocompatible electrodes represents an active field of research as it pursues both fundamental and technological interests. In this context, adsorption and redox properties of cytochrome c (Cyt) on different electrode surfaces have been extensively reported, although in some cases with contradictory results. Here we report a SERR spectroelectrochemical study of the adsorption and electron transfer behaviour of the basic protein Cyt on electrodes coated with amino-terminated monolayers. The obtained results show that inorganic phosphate (Pi) and ATP anions are able to mediate high affinity binding of the protein with preservation of the native structure and rendering an average orientation that guarantees efficient pathways for direct electron transfer. These findings aid the design of Cyt-based bioelectronic devices and understanding the modulation by Pi and ATP of physiological functions of Cyt.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7817
Capdevila, Daiana Andrea; Marmisollé, Waldemar Alejandro; Williams, Federico Jose; Murgida, Daniel Horacio; Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study; Royal Society Of Chemistry; Physical Chemistry Chemical Physics; 15; 15; 3-2013; 5386-5394
1463-9076
url http://hdl.handle.net/11336/7817
identifier_str_mv Capdevila, Daiana Andrea; Marmisollé, Waldemar Alejandro; Williams, Federico Jose; Murgida, Daniel Horacio; Phosphate Mediated Adsorption and Electron Transfer of Cytochrome C. A Time-Resolved SERR Spectroelectrochemical Study; Royal Society Of Chemistry; Physical Chemistry Chemical Physics; 15; 15; 3-2013; 5386-5394
1463-9076
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c2cp42044a
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2013/cp/c2cp42044a#!divAbstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society Of Chemistry
publisher.none.fl_str_mv Royal Society Of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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