Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis

Autores
Esteban, Laura Emilia; Temprana, Carlos Facundo; Argüelles, M. H.; Glikmann, G.; Castello, Alejandro Andrés
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection.
Fil: Esteban, Laura Emilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argüelles, M. H.. Universidad Nacional de Quilmes; Argentina
Fil: Glikmann, G.. Universidad Nacional de Quilmes; Argentina
Fil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes; Argentina
Materia
ROTAVIRUS
L. LACTIS
VP6
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27797

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network_name_str CONICET Digital (CONICET)
spelling Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactisEsteban, Laura EmiliaTemprana, Carlos FacundoArgüelles, M. H.Glikmann, G.Castello, Alejandro AndrésROTAVIRUSL. LACTISVP6https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection.Fil: Esteban, Laura Emilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Argüelles, M. H.. Universidad Nacional de Quilmes; ArgentinaFil: Glikmann, G.. Universidad Nacional de Quilmes; ArgentinaFil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes; ArgentinaHindawi Publishing Corporation2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27797Esteban, Laura Emilia; Temprana, Carlos Facundo; Argüelles, M. H.; Glikmann, G.; Castello, Alejandro Andrés; Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis; Hindawi Publishing Corporation; BioMed Research International; 2013; 7-2013; 1-8; 2985982314-61332314-6141CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/298598/info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/298598info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:27Zoai:ri.conicet.gov.ar:11336/27797instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:27.655CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
title Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
spellingShingle Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
Esteban, Laura Emilia
ROTAVIRUS
L. LACTIS
VP6
title_short Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
title_full Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
title_fullStr Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
title_full_unstemmed Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
title_sort Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
dc.creator.none.fl_str_mv Esteban, Laura Emilia
Temprana, Carlos Facundo
Argüelles, M. H.
Glikmann, G.
Castello, Alejandro Andrés
author Esteban, Laura Emilia
author_facet Esteban, Laura Emilia
Temprana, Carlos Facundo
Argüelles, M. H.
Glikmann, G.
Castello, Alejandro Andrés
author_role author
author2 Temprana, Carlos Facundo
Argüelles, M. H.
Glikmann, G.
Castello, Alejandro Andrés
author2_role author
author
author
author
dc.subject.none.fl_str_mv ROTAVIRUS
L. LACTIS
VP6
topic ROTAVIRUS
L. LACTIS
VP6
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection.
Fil: Esteban, Laura Emilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argüelles, M. H.. Universidad Nacional de Quilmes; Argentina
Fil: Glikmann, G.. Universidad Nacional de Quilmes; Argentina
Fil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes; Argentina
description Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27797
Esteban, Laura Emilia; Temprana, Carlos Facundo; Argüelles, M. H.; Glikmann, G.; Castello, Alejandro Andrés; Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis; Hindawi Publishing Corporation; BioMed Research International; 2013; 7-2013; 1-8; 298598
2314-6133
2314-6141
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27797
identifier_str_mv Esteban, Laura Emilia; Temprana, Carlos Facundo; Argüelles, M. H.; Glikmann, G.; Castello, Alejandro Andrés; Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis; Hindawi Publishing Corporation; BioMed Research International; 2013; 7-2013; 1-8; 298598
2314-6133
2314-6141
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/298598/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/298598
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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