Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis
- Autores
- Esteban, Laura Emilia; Temprana, Carlos Facundo; Argüelles, M. H.; Glikmann, G.; Castello, Alejandro Andrés
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection.
Fil: Esteban, Laura Emilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argüelles, M. H.. Universidad Nacional de Quilmes; Argentina
Fil: Glikmann, G.. Universidad Nacional de Quilmes; Argentina
Fil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes; Argentina - Materia
-
ROTAVIRUS
L. LACTIS
VP6 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/27797
Ver los metadatos del registro completo
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Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactisEsteban, Laura EmiliaTemprana, Carlos FacundoArgüelles, M. H.Glikmann, G.Castello, Alejandro AndrésROTAVIRUSL. LACTISVP6https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection.Fil: Esteban, Laura Emilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Argüelles, M. H.. Universidad Nacional de Quilmes; ArgentinaFil: Glikmann, G.. Universidad Nacional de Quilmes; ArgentinaFil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes; ArgentinaHindawi Publishing Corporation2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27797Esteban, Laura Emilia; Temprana, Carlos Facundo; Argüelles, M. H.; Glikmann, G.; Castello, Alejandro Andrés; Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis; Hindawi Publishing Corporation; BioMed Research International; 2013; 7-2013; 1-8; 2985982314-61332314-6141CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/298598/info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/298598info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:27Zoai:ri.conicet.gov.ar:11336/27797instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:27.655CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis |
title |
Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis |
spellingShingle |
Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis Esteban, Laura Emilia ROTAVIRUS L. LACTIS VP6 |
title_short |
Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis |
title_full |
Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis |
title_fullStr |
Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis |
title_full_unstemmed |
Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis |
title_sort |
Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis |
dc.creator.none.fl_str_mv |
Esteban, Laura Emilia Temprana, Carlos Facundo Argüelles, M. H. Glikmann, G. Castello, Alejandro Andrés |
author |
Esteban, Laura Emilia |
author_facet |
Esteban, Laura Emilia Temprana, Carlos Facundo Argüelles, M. H. Glikmann, G. Castello, Alejandro Andrés |
author_role |
author |
author2 |
Temprana, Carlos Facundo Argüelles, M. H. Glikmann, G. Castello, Alejandro Andrés |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
ROTAVIRUS L. LACTIS VP6 |
topic |
ROTAVIRUS L. LACTIS VP6 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection. Fil: Esteban, Laura Emilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Argüelles, M. H.. Universidad Nacional de Quilmes; Argentina Fil: Glikmann, G.. Universidad Nacional de Quilmes; Argentina Fil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes; Argentina |
description |
Group A rotaviruses are the major etiologic agents of acute gastroenteritis worldwide in children and young animals. Among its structural proteins, VP6 is the most immunogenic and is highly conserved within this group. Lactococcus lactis is a food-grade, Gram-positive, and nonpathogenic lactic acid bacteria that has already been explored as a mucosal delivery system of heterologous antigens. In this work, the nisin-controlled expression system was used to display the VP6 protein at the cell surface of L. lactis. Conditions for optimal gene expression were established by testing different nisin concentrations, cell density at induction, and incubation times after induction. Cytoplasmic and cell wall protein extracts were analyzed by Western blot and surface expression was confirmed by flow cytometry. Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis. Furthermore, the humoral response of mice immunized with recombinant L. lactis was evaluated and the displayed recombinant VP6 protein proved to be immunogenic. In conclusion, this is the first report of displaying VP6 protein on the surface of L. lactis to induce a specific immune response against rotavirus. These results provide the basis for further evaluation of this VP6-displaying L. lactis as a mucosal delivery vector in a mouse model of rotavirus infection. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/27797 Esteban, Laura Emilia; Temprana, Carlos Facundo; Argüelles, M. H.; Glikmann, G.; Castello, Alejandro Andrés; Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis; Hindawi Publishing Corporation; BioMed Research International; 2013; 7-2013; 1-8; 298598 2314-6133 2314-6141 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/27797 |
identifier_str_mv |
Esteban, Laura Emilia; Temprana, Carlos Facundo; Argüelles, M. H.; Glikmann, G.; Castello, Alejandro Andrés; Antigenicity and immunogenicity of Rotavirus VP6 rrotein expressed on the surface of Lactococcus lactis; Hindawi Publishing Corporation; BioMed Research International; 2013; 7-2013; 1-8; 298598 2314-6133 2314-6141 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/298598/ info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/298598 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614385234870272 |
score |
13.070432 |