Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation

Autores
Bivi, Nicoletta; Condon, Keith W.; Allen, Matthew R.; Farlow, Nathan; Passeri, Giovanni; Brun, Lucas Ricardo Martín; Rhee, Yumie; Bellido, Teresita; Plotkin, Lilian I.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Connexin43 (Cx43) mediates osteocyte communication with other cells and with the extracellular milieu and regulates osteoblastic cell signaling and gene expression. We now report that mice lacking Cx43 in osteoblasts/osteocytes or only in osteocytes (Cx43Ot mice)exhibit increased osteocyte apoptosis, endocortical resorptionand periosteal bone formation, resulting in higher marrow cavityand total tissueareas measured at the femoral mid-diaphysis.Blockade of resorption reversed the increasedmarrow cavity but not total tissue area, demonstrating that endocortical resorption andperiosteal apposition are independently regulated.Anatomical mappingof apoptotic osteocytes,osteocytic protein expression, and resorption and formation,suggeststhat Cx43 controls osteoclast and osteoblast activity by regulating osteoprotegerin and sclerostinlevels, respectively, in osteocytes located in specific areas of the cortex.Whereas empty lacunae and living osteocytes lacking osteoprotegerinwere distributed throughout cortical bone in Cx43Ot mice, apoptotic osteocyteswere preferentially located in areas containing osteoclasts, suggesting that osteoclast recruitment requires active signaling from dying osteocytes.Furthermore, Cx43 deletion in cultured osteocytic cells resulted in increased apoptosis and decreased osteoprotegerin expression. Thus, Cx43 is essential in a cell-autonomous fashionin vivo and in vitrofor osteocyte survivaland for controlling the expression of osteocytic genesthat affectosteoclast and osteoblast function.
Fil: Bivi, Nicoletta. Indiana University; Estados Unidos
Fil: Condon, Keith W.. Indiana University; Estados Unidos
Fil: Allen, Matthew R.. Indiana University; Estados Unidos
Fil: Farlow, Nathan. Indiana University; Estados Unidos
Fil: Passeri, Giovanni. Indiana University; Estados Unidos
Fil: Brun, Lucas Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Indiana University; Estados Unidos
Fil: Rhee, Yumie. Indiana University; Estados Unidos
Fil: Bellido, Teresita. Indiana University; Estados Unidos
Fil: Plotkin, Lilian I.. Indiana University; Estados Unidos
Materia
Connexin 43
Apoptosis
Osteocyte
Osteoblast
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269363
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/269363
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formationBivi, NicolettaCondon, Keith W.Allen, Matthew R.Farlow, NathanPasseri, GiovanniBrun, Lucas Ricardo MartínRhee, YumieBellido, TeresitaPlotkin, Lilian I.Connexin 43ApoptosisOsteocyteOsteoblasthttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Connexin43 (Cx43) mediates osteocyte communication with other cells and with the extracellular milieu and regulates osteoblastic cell signaling and gene expression. We now report that mice lacking Cx43 in osteoblasts/osteocytes or only in osteocytes (Cx43Ot mice)exhibit increased osteocyte apoptosis, endocortical resorptionand periosteal bone formation, resulting in higher marrow cavityand total tissueareas measured at the femoral mid-diaphysis.Blockade of resorption reversed the increasedmarrow cavity but not total tissue area, demonstrating that endocortical resorption andperiosteal apposition are independently regulated.Anatomical mappingof apoptotic osteocytes,osteocytic protein expression, and resorption and formation,suggeststhat Cx43 controls osteoclast and osteoblast activity by regulating osteoprotegerin and sclerostinlevels, respectively, in osteocytes located in specific areas of the cortex.Whereas empty lacunae and living osteocytes lacking osteoprotegerinwere distributed throughout cortical bone in Cx43Ot mice, apoptotic osteocyteswere preferentially located in areas containing osteoclasts, suggesting that osteoclast recruitment requires active signaling from dying osteocytes.Furthermore, Cx43 deletion in cultured osteocytic cells resulted in increased apoptosis and decreased osteoprotegerin expression. Thus, Cx43 is essential in a cell-autonomous fashionin vivo and in vitrofor osteocyte survivaland for controlling the expression of osteocytic genesthat affectosteoclast and osteoblast function.Fil: Bivi, Nicoletta. Indiana University; Estados UnidosFil: Condon, Keith W.. Indiana University; Estados UnidosFil: Allen, Matthew R.. Indiana University; Estados UnidosFil: Farlow, Nathan. Indiana University; Estados UnidosFil: Passeri, Giovanni. Indiana University; Estados UnidosFil: Brun, Lucas Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Indiana University; Estados UnidosFil: Rhee, Yumie. Indiana University; Estados UnidosFil: Bellido, Teresita. Indiana University; Estados UnidosFil: Plotkin, Lilian I.. Indiana University; Estados UnidosAmerican Society for Bone and Mineral Research2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269363Bivi, Nicoletta; Condon, Keith W.; Allen, Matthew R.; Farlow, Nathan; Passeri, Giovanni; et al.; Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation; American Society for Bone and Mineral Research; Journal of Bone and Mineral Research; 27; 2; 1-2012; 374-3890884-0431CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jbmr/article-abstract/27/2/374/7598351info:eu-repo/semantics/altIdentifier/doi/10.1002/jbmr.548info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:56Zoai:ri.conicet.gov.ar:11336/269363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:56.952CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation
title Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation
spellingShingle Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation
Bivi, Nicoletta
Connexin 43
Apoptosis
Osteocyte
Osteoblast
title_short Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation
title_full Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation
title_fullStr Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation
title_full_unstemmed Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation
title_sort Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation
dc.creator.none.fl_str_mv Bivi, Nicoletta
Condon, Keith W.
Allen, Matthew R.
Farlow, Nathan
Passeri, Giovanni
Brun, Lucas Ricardo Martín
Rhee, Yumie
Bellido, Teresita
Plotkin, Lilian I.
author Bivi, Nicoletta
author_facet Bivi, Nicoletta
Condon, Keith W.
Allen, Matthew R.
Farlow, Nathan
Passeri, Giovanni
Brun, Lucas Ricardo Martín
Rhee, Yumie
Bellido, Teresita
Plotkin, Lilian I.
author_role author
author2 Condon, Keith W.
Allen, Matthew R.
Farlow, Nathan
Passeri, Giovanni
Brun, Lucas Ricardo Martín
Rhee, Yumie
Bellido, Teresita
Plotkin, Lilian I.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Connexin 43
Apoptosis
Osteocyte
Osteoblast
topic Connexin 43
Apoptosis
Osteocyte
Osteoblast
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Connexin43 (Cx43) mediates osteocyte communication with other cells and with the extracellular milieu and regulates osteoblastic cell signaling and gene expression. We now report that mice lacking Cx43 in osteoblasts/osteocytes or only in osteocytes (Cx43Ot mice)exhibit increased osteocyte apoptosis, endocortical resorptionand periosteal bone formation, resulting in higher marrow cavityand total tissueareas measured at the femoral mid-diaphysis.Blockade of resorption reversed the increasedmarrow cavity but not total tissue area, demonstrating that endocortical resorption andperiosteal apposition are independently regulated.Anatomical mappingof apoptotic osteocytes,osteocytic protein expression, and resorption and formation,suggeststhat Cx43 controls osteoclast and osteoblast activity by regulating osteoprotegerin and sclerostinlevels, respectively, in osteocytes located in specific areas of the cortex.Whereas empty lacunae and living osteocytes lacking osteoprotegerinwere distributed throughout cortical bone in Cx43Ot mice, apoptotic osteocyteswere preferentially located in areas containing osteoclasts, suggesting that osteoclast recruitment requires active signaling from dying osteocytes.Furthermore, Cx43 deletion in cultured osteocytic cells resulted in increased apoptosis and decreased osteoprotegerin expression. Thus, Cx43 is essential in a cell-autonomous fashionin vivo and in vitrofor osteocyte survivaland for controlling the expression of osteocytic genesthat affectosteoclast and osteoblast function.
Fil: Bivi, Nicoletta. Indiana University; Estados Unidos
Fil: Condon, Keith W.. Indiana University; Estados Unidos
Fil: Allen, Matthew R.. Indiana University; Estados Unidos
Fil: Farlow, Nathan. Indiana University; Estados Unidos
Fil: Passeri, Giovanni. Indiana University; Estados Unidos
Fil: Brun, Lucas Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Indiana University; Estados Unidos
Fil: Rhee, Yumie. Indiana University; Estados Unidos
Fil: Bellido, Teresita. Indiana University; Estados Unidos
Fil: Plotkin, Lilian I.. Indiana University; Estados Unidos
description Connexin43 (Cx43) mediates osteocyte communication with other cells and with the extracellular milieu and regulates osteoblastic cell signaling and gene expression. We now report that mice lacking Cx43 in osteoblasts/osteocytes or only in osteocytes (Cx43Ot mice)exhibit increased osteocyte apoptosis, endocortical resorptionand periosteal bone formation, resulting in higher marrow cavityand total tissueareas measured at the femoral mid-diaphysis.Blockade of resorption reversed the increasedmarrow cavity but not total tissue area, demonstrating that endocortical resorption andperiosteal apposition are independently regulated.Anatomical mappingof apoptotic osteocytes,osteocytic protein expression, and resorption and formation,suggeststhat Cx43 controls osteoclast and osteoblast activity by regulating osteoprotegerin and sclerostinlevels, respectively, in osteocytes located in specific areas of the cortex.Whereas empty lacunae and living osteocytes lacking osteoprotegerinwere distributed throughout cortical bone in Cx43Ot mice, apoptotic osteocyteswere preferentially located in areas containing osteoclasts, suggesting that osteoclast recruitment requires active signaling from dying osteocytes.Furthermore, Cx43 deletion in cultured osteocytic cells resulted in increased apoptosis and decreased osteoprotegerin expression. Thus, Cx43 is essential in a cell-autonomous fashionin vivo and in vitrofor osteocyte survivaland for controlling the expression of osteocytic genesthat affectosteoclast and osteoblast function.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269363
Bivi, Nicoletta; Condon, Keith W.; Allen, Matthew R.; Farlow, Nathan; Passeri, Giovanni; et al.; Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation; American Society for Bone and Mineral Research; Journal of Bone and Mineral Research; 27; 2; 1-2012; 374-389
0884-0431
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269363
identifier_str_mv Bivi, Nicoletta; Condon, Keith W.; Allen, Matthew R.; Farlow, Nathan; Passeri, Giovanni; et al.; Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation; American Society for Bone and Mineral Research; Journal of Bone and Mineral Research; 27; 2; 1-2012; 374-389
0884-0431
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jbmr/article-abstract/27/2/374/7598351
info:eu-repo/semantics/altIdentifier/doi/10.1002/jbmr.548
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Bone and Mineral Research
publisher.none.fl_str_mv American Society for Bone and Mineral Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269776161800192
score 13.13397

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