Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice

Autores
Bivi, Nicoletta; Pacheco Costa, Rafael; Brun, Lucas Ricardo Martín; Murphy, Thomas R.; Farlow, Nathan R.; Robling, Alexander G.; Bellido, Teresita; Plotkin, Lilian I.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The osteocyte network is crucial for the response of bone to mechanical force. Within this network, connexin43 (Cx43) is thought to mediate the communication of osteocytes and osteoblasts among themselves and the exchange of small molecules with the extracellular milieu. Despite recent advances in understanding Cx43 role for the response of bone cells to mechanical stimulation, the contribution of Cx43 specifically in osteocytes to mechanotransduction in vivo is not well-known. We examined the anabolic response to ulnar axial loading of mice lacking Cx43 in osteocytes (Cx43DOt). Loading induced a greater increase in periosteal bone formation rate in Cx43DOt mice compared to control littermates, resulting from higher mineralizing surface and enhanced mineral apposition rate. Expression of b-catenin protein, a molecule implicated in mechanotransduction, was higher in bones from Cx43DOt mice, compared to littermate controls. In addition, MLO-Y4 osteocytic cells knocked-down for Cx43 exhibited higher b-catenin protein expression and enhanced response to mechanical stimulation. These findings suggest that osteocytes lacking Cx43 are “primed” to respond to mechanical stimulation and that absence of Cx43 in osteocytes unleashes bone formation, by a mechanism that might involve accumulation of b-catenin.
Fil: Bivi, Nicoletta. Indiana University; Estados Unidos
Fil: Pacheco Costa, Rafael. Indiana University; Estados Unidos. Universidade de Sao Paulo; Brasil
Fil: Brun, Lucas Ricardo Martín. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Murphy, Thomas R.. Indiana University; Estados Unidos
Fil: Farlow, Nathan R.. Indiana University; Estados Unidos
Fil: Robling, Alexander G.. Indiana University; Estados Unidos
Fil: Bellido, Teresita. Indiana University; Estados Unidos
Fil: Plotkin, Lilian I.. Indiana University; Estados Unidos
Materia
Connexin 43
Bone Formation
Osteocyte
Mechanical Loading
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21682
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21682
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in miceBivi, NicolettaPacheco Costa, RafaelBrun, Lucas Ricardo MartínMurphy, Thomas R.Farlow, Nathan R.Robling, Alexander G.Bellido, TeresitaPlotkin, Lilian I.Connexin 43Bone FormationOsteocyteMechanical Loadinghttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The osteocyte network is crucial for the response of bone to mechanical force. Within this network, connexin43 (Cx43) is thought to mediate the communication of osteocytes and osteoblasts among themselves and the exchange of small molecules with the extracellular milieu. Despite recent advances in understanding Cx43 role for the response of bone cells to mechanical stimulation, the contribution of Cx43 specifically in osteocytes to mechanotransduction in vivo is not well-known. We examined the anabolic response to ulnar axial loading of mice lacking Cx43 in osteocytes (Cx43DOt). Loading induced a greater increase in periosteal bone formation rate in Cx43DOt mice compared to control littermates, resulting from higher mineralizing surface and enhanced mineral apposition rate. Expression of b-catenin protein, a molecule implicated in mechanotransduction, was higher in bones from Cx43DOt mice, compared to littermate controls. In addition, MLO-Y4 osteocytic cells knocked-down for Cx43 exhibited higher b-catenin protein expression and enhanced response to mechanical stimulation. These findings suggest that osteocytes lacking Cx43 are “primed” to respond to mechanical stimulation and that absence of Cx43 in osteocytes unleashes bone formation, by a mechanism that might involve accumulation of b-catenin.Fil: Bivi, Nicoletta. Indiana University; Estados UnidosFil: Pacheco Costa, Rafael. Indiana University; Estados Unidos. Universidade de Sao Paulo; BrasilFil: Brun, Lucas Ricardo Martín. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Murphy, Thomas R.. Indiana University; Estados UnidosFil: Farlow, Nathan R.. Indiana University; Estados UnidosFil: Robling, Alexander G.. Indiana University; Estados UnidosFil: Bellido, Teresita. Indiana University; Estados UnidosFil: Plotkin, Lilian I.. Indiana University; Estados UnidosWiley2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21682Bivi, Nicoletta; Pacheco Costa, Rafael; Brun, Lucas Ricardo Martín; Murphy, Thomas R.; Farlow, Nathan R.; et al.; Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice; Wiley; Journal Of Orthopaedic Research; 31; 7; 3-2013; 1075-10810736-0266CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jor.22341info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jor.22341/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:08:56Zoai:ri.conicet.gov.ar:11336/21682instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:08:56.904CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice
title Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice
spellingShingle Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice
Bivi, Nicoletta
Connexin 43
Bone Formation
Osteocyte
Mechanical Loading
title_short Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice
title_full Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice
title_fullStr Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice
title_full_unstemmed Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice
title_sort Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice
dc.creator.none.fl_str_mv Bivi, Nicoletta
Pacheco Costa, Rafael
Brun, Lucas Ricardo Martín
Murphy, Thomas R.
Farlow, Nathan R.
Robling, Alexander G.
Bellido, Teresita
Plotkin, Lilian I.
author Bivi, Nicoletta
author_facet Bivi, Nicoletta
Pacheco Costa, Rafael
Brun, Lucas Ricardo Martín
Murphy, Thomas R.
Farlow, Nathan R.
Robling, Alexander G.
Bellido, Teresita
Plotkin, Lilian I.
author_role author
author2 Pacheco Costa, Rafael
Brun, Lucas Ricardo Martín
Murphy, Thomas R.
Farlow, Nathan R.
Robling, Alexander G.
Bellido, Teresita
Plotkin, Lilian I.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Connexin 43
Bone Formation
Osteocyte
Mechanical Loading
topic Connexin 43
Bone Formation
Osteocyte
Mechanical Loading
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The osteocyte network is crucial for the response of bone to mechanical force. Within this network, connexin43 (Cx43) is thought to mediate the communication of osteocytes and osteoblasts among themselves and the exchange of small molecules with the extracellular milieu. Despite recent advances in understanding Cx43 role for the response of bone cells to mechanical stimulation, the contribution of Cx43 specifically in osteocytes to mechanotransduction in vivo is not well-known. We examined the anabolic response to ulnar axial loading of mice lacking Cx43 in osteocytes (Cx43DOt). Loading induced a greater increase in periosteal bone formation rate in Cx43DOt mice compared to control littermates, resulting from higher mineralizing surface and enhanced mineral apposition rate. Expression of b-catenin protein, a molecule implicated in mechanotransduction, was higher in bones from Cx43DOt mice, compared to littermate controls. In addition, MLO-Y4 osteocytic cells knocked-down for Cx43 exhibited higher b-catenin protein expression and enhanced response to mechanical stimulation. These findings suggest that osteocytes lacking Cx43 are “primed” to respond to mechanical stimulation and that absence of Cx43 in osteocytes unleashes bone formation, by a mechanism that might involve accumulation of b-catenin.
Fil: Bivi, Nicoletta. Indiana University; Estados Unidos
Fil: Pacheco Costa, Rafael. Indiana University; Estados Unidos. Universidade de Sao Paulo; Brasil
Fil: Brun, Lucas Ricardo Martín. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Murphy, Thomas R.. Indiana University; Estados Unidos
Fil: Farlow, Nathan R.. Indiana University; Estados Unidos
Fil: Robling, Alexander G.. Indiana University; Estados Unidos
Fil: Bellido, Teresita. Indiana University; Estados Unidos
Fil: Plotkin, Lilian I.. Indiana University; Estados Unidos
description The osteocyte network is crucial for the response of bone to mechanical force. Within this network, connexin43 (Cx43) is thought to mediate the communication of osteocytes and osteoblasts among themselves and the exchange of small molecules with the extracellular milieu. Despite recent advances in understanding Cx43 role for the response of bone cells to mechanical stimulation, the contribution of Cx43 specifically in osteocytes to mechanotransduction in vivo is not well-known. We examined the anabolic response to ulnar axial loading of mice lacking Cx43 in osteocytes (Cx43DOt). Loading induced a greater increase in periosteal bone formation rate in Cx43DOt mice compared to control littermates, resulting from higher mineralizing surface and enhanced mineral apposition rate. Expression of b-catenin protein, a molecule implicated in mechanotransduction, was higher in bones from Cx43DOt mice, compared to littermate controls. In addition, MLO-Y4 osteocytic cells knocked-down for Cx43 exhibited higher b-catenin protein expression and enhanced response to mechanical stimulation. These findings suggest that osteocytes lacking Cx43 are “primed” to respond to mechanical stimulation and that absence of Cx43 in osteocytes unleashes bone formation, by a mechanism that might involve accumulation of b-catenin.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21682
Bivi, Nicoletta; Pacheco Costa, Rafael; Brun, Lucas Ricardo Martín; Murphy, Thomas R.; Farlow, Nathan R.; et al.; Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice; Wiley; Journal Of Orthopaedic Research; 31; 7; 3-2013; 1075-1081
0736-0266
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21682
identifier_str_mv Bivi, Nicoletta; Pacheco Costa, Rafael; Brun, Lucas Ricardo Martín; Murphy, Thomas R.; Farlow, Nathan R.; et al.; Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice; Wiley; Journal Of Orthopaedic Research; 31; 7; 3-2013; 1075-1081
0736-0266
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jor.22341
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jor.22341/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.891075

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