Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port o...

Autores
Corti Monzón, Georgina de la Paz; Nisenbaum, Melina; Peressutti, Silvia Rita Peressutti; Junca, Howard; García Bonilla, Erika; Murialdo, Silvia Elena
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present work provides the first knowledge about the diversity and functionality of the microorganisms occurring in oily bilge wastes, from the port of Mar del Plata, Argentina. We used a culture independent approach, based on massive sequencing of the 16S rRNA gene (Illumina), to analyze the microbiome structure from different oily bilge wastes. To gain insight into functional biomarkers for hydrocarbon (HC) degradation in these wastes, the occurrence of alkB genes in the degrading bacteria communities was also investigated trough PCR-DGGE method. The microbial compositions were determined in bilge waste oily phase (BWOP) samples from a deep-sea, a fishing ship, a dredge ship, a small coastal vessel, and an open pool, where bilge wastes from Mar del Plata’s vessels are deposited. A core microbiome with OTUs classified as bacterial types associated with HC biodegradation was found across all samples. Seven highly predominant and stable bacterial types accounted for 82% of the relative composition determined in all the BWOP samples. These results indicate that oily bilge is a strong selector for bacteria having common taxonomical and functional features, independent of the vessel of collection. Alkane degradation marker gene alkB PCR-DGGE fingerprinting showed distinct diversity patterns among the samples. Our analysis showed that there is a well-defined bacterial assemblage able to withstand and thrive in the harsh conditions of the oily bilge wastes. Also, a well-established aliphatic biodegradation potential marker was detected, indicating that it could promptly be used for the generation of new bioremediation technologies.
Fil: Corti Monzón, Georgina de la Paz. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Nisenbaum, Melina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Peressutti, Silvia Rita Peressutti. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Junca, Howard. No especifíca;
Fil: García Bonilla, Erika. No especifíca;
Fil: Murialdo, Silvia Elena. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Materia
ALKANE MONOOXYGENASE GENE
BILGE WASTE
HYDROCARBON
MICROBIOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/164710
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, ArgentinaCorti Monzón, Georgina de la PazNisenbaum, MelinaPeressutti, Silvia Rita PeressuttiJunca, HowardGarcía Bonilla, ErikaMurialdo, Silvia ElenaALKANE MONOOXYGENASE GENEBILGE WASTEHYDROCARBONMICROBIOMEhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The present work provides the first knowledge about the diversity and functionality of the microorganisms occurring in oily bilge wastes, from the port of Mar del Plata, Argentina. We used a culture independent approach, based on massive sequencing of the 16S rRNA gene (Illumina), to analyze the microbiome structure from different oily bilge wastes. To gain insight into functional biomarkers for hydrocarbon (HC) degradation in these wastes, the occurrence of alkB genes in the degrading bacteria communities was also investigated trough PCR-DGGE method. The microbial compositions were determined in bilge waste oily phase (BWOP) samples from a deep-sea, a fishing ship, a dredge ship, a small coastal vessel, and an open pool, where bilge wastes from Mar del Plata’s vessels are deposited. A core microbiome with OTUs classified as bacterial types associated with HC biodegradation was found across all samples. Seven highly predominant and stable bacterial types accounted for 82% of the relative composition determined in all the BWOP samples. These results indicate that oily bilge is a strong selector for bacteria having common taxonomical and functional features, independent of the vessel of collection. Alkane degradation marker gene alkB PCR-DGGE fingerprinting showed distinct diversity patterns among the samples. Our analysis showed that there is a well-defined bacterial assemblage able to withstand and thrive in the harsh conditions of the oily bilge wastes. Also, a well-established aliphatic biodegradation potential marker was detected, indicating that it could promptly be used for the generation of new bioremediation technologies.Fil: Corti Monzón, Georgina de la Paz. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Nisenbaum, Melina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Peressutti, Silvia Rita Peressutti. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Junca, Howard. No especifíca;Fil: García Bonilla, Erika. No especifíca;Fil: Murialdo, Silvia Elena. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaSpringer2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/164710Corti Monzón, Georgina de la Paz; Nisenbaum, Melina; Peressutti, Silvia Rita Peressutti; Junca, Howard; García Bonilla, Erika; et al.; Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina; Springer; Water, Air and Soil Pollution; 232; 7; 7-2021; 1-190049-6979CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-021-05239-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:32Zoai:ri.conicet.gov.ar:11336/164710instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:32.312CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina
title Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina
spellingShingle Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina
Corti Monzón, Georgina de la Paz
ALKANE MONOOXYGENASE GENE
BILGE WASTE
HYDROCARBON
MICROBIOME
title_short Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina
title_full Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina
title_fullStr Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina
title_full_unstemmed Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina
title_sort Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina
dc.creator.none.fl_str_mv Corti Monzón, Georgina de la Paz
Nisenbaum, Melina
Peressutti, Silvia Rita Peressutti
Junca, Howard
García Bonilla, Erika
Murialdo, Silvia Elena
author Corti Monzón, Georgina de la Paz
author_facet Corti Monzón, Georgina de la Paz
Nisenbaum, Melina
Peressutti, Silvia Rita Peressutti
Junca, Howard
García Bonilla, Erika
Murialdo, Silvia Elena
author_role author
author2 Nisenbaum, Melina
Peressutti, Silvia Rita Peressutti
Junca, Howard
García Bonilla, Erika
Murialdo, Silvia Elena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALKANE MONOOXYGENASE GENE
BILGE WASTE
HYDROCARBON
MICROBIOME
topic ALKANE MONOOXYGENASE GENE
BILGE WASTE
HYDROCARBON
MICROBIOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The present work provides the first knowledge about the diversity and functionality of the microorganisms occurring in oily bilge wastes, from the port of Mar del Plata, Argentina. We used a culture independent approach, based on massive sequencing of the 16S rRNA gene (Illumina), to analyze the microbiome structure from different oily bilge wastes. To gain insight into functional biomarkers for hydrocarbon (HC) degradation in these wastes, the occurrence of alkB genes in the degrading bacteria communities was also investigated trough PCR-DGGE method. The microbial compositions were determined in bilge waste oily phase (BWOP) samples from a deep-sea, a fishing ship, a dredge ship, a small coastal vessel, and an open pool, where bilge wastes from Mar del Plata’s vessels are deposited. A core microbiome with OTUs classified as bacterial types associated with HC biodegradation was found across all samples. Seven highly predominant and stable bacterial types accounted for 82% of the relative composition determined in all the BWOP samples. These results indicate that oily bilge is a strong selector for bacteria having common taxonomical and functional features, independent of the vessel of collection. Alkane degradation marker gene alkB PCR-DGGE fingerprinting showed distinct diversity patterns among the samples. Our analysis showed that there is a well-defined bacterial assemblage able to withstand and thrive in the harsh conditions of the oily bilge wastes. Also, a well-established aliphatic biodegradation potential marker was detected, indicating that it could promptly be used for the generation of new bioremediation technologies.
Fil: Corti Monzón, Georgina de la Paz. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Nisenbaum, Melina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Peressutti, Silvia Rita Peressutti. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Junca, Howard. No especifíca;
Fil: García Bonilla, Erika. No especifíca;
Fil: Murialdo, Silvia Elena. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
description The present work provides the first knowledge about the diversity and functionality of the microorganisms occurring in oily bilge wastes, from the port of Mar del Plata, Argentina. We used a culture independent approach, based on massive sequencing of the 16S rRNA gene (Illumina), to analyze the microbiome structure from different oily bilge wastes. To gain insight into functional biomarkers for hydrocarbon (HC) degradation in these wastes, the occurrence of alkB genes in the degrading bacteria communities was also investigated trough PCR-DGGE method. The microbial compositions were determined in bilge waste oily phase (BWOP) samples from a deep-sea, a fishing ship, a dredge ship, a small coastal vessel, and an open pool, where bilge wastes from Mar del Plata’s vessels are deposited. A core microbiome with OTUs classified as bacterial types associated with HC biodegradation was found across all samples. Seven highly predominant and stable bacterial types accounted for 82% of the relative composition determined in all the BWOP samples. These results indicate that oily bilge is a strong selector for bacteria having common taxonomical and functional features, independent of the vessel of collection. Alkane degradation marker gene alkB PCR-DGGE fingerprinting showed distinct diversity patterns among the samples. Our analysis showed that there is a well-defined bacterial assemblage able to withstand and thrive in the harsh conditions of the oily bilge wastes. Also, a well-established aliphatic biodegradation potential marker was detected, indicating that it could promptly be used for the generation of new bioremediation technologies.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/164710
Corti Monzón, Georgina de la Paz; Nisenbaum, Melina; Peressutti, Silvia Rita Peressutti; Junca, Howard; García Bonilla, Erika; et al.; Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina; Springer; Water, Air and Soil Pollution; 232; 7; 7-2021; 1-19
0049-6979
CONICET Digital
CONICET
url http://hdl.handle.net/11336/164710
identifier_str_mv Corti Monzón, Georgina de la Paz; Nisenbaum, Melina; Peressutti, Silvia Rita Peressutti; Junca, Howard; García Bonilla, Erika; et al.; Oily Bilge Wastes Harbor a Set of Persistent Hydrocarbonoclastic Bacteria Accompanied by a Variable alkB Gene Composition in Marine Vessel Samples from Southwestern Atlantic Port of Mar del Plata, Argentina; Springer; Water, Air and Soil Pollution; 232; 7; 7-2021; 1-19
0049-6979
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-021-05239-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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