Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain
- Autores
- Cisternas, Carla Daniela; Cortes, Laura R.; Bruggeman, Emily C.; Yao, Bing; Forger, Nancy G.
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- DNA methylation is dynamically modulated during postnatal brain development, and plays a key role in neuronal lineage commitment. This epigenetic mark has also recently been implicated in the development of neural sex differences, many of which are found in the hypothalamus. The level of DNA methylation depends on a balance between the placement of methyl marks by DNA methyltransferases (Dnmts) and their removal, which is catalyzed by ten-eleven translocation (Tet) methylcytosine dioxygenases. Here, we examined developmental changes and sex differences in the expression of Tet and Dnmt enzymes from birth to adulthood in two hypothalamic regions (the preoptic area and ventromedial nucleus) and the hippocampus of mice. We found highest expression of all Tet enzymes (Tet1, Tet2, Tet3) and Dnmts (Dnmt1, Dnmt3a, Dnmt3b) in newborns, despite the fact that global methylation and hydroxymethylation were at their lowest levels at birth. Expression of the Dnmt co-activator, Dnmt3l, followed a pattern opposite to that of the canonical Dnmts (i.e., was very low in newborns and increased with age). Tet enzyme activity was much higher at birth than at weaning in both the hypothalamus and hippocampus, mirroring developmental changes in gene expression. Sex differences in Tet enzyme expression were seen in all brain regions examined during the first week of life, whereas Dnmt expression was more balanced between the sexes. Neonatal testosterone treatment of females only partially masculinized enzyme expression. Thus, Tet expression and activity are elevated during neonatal brain development, and may play important roles in sexual differentiation of the brain.
Fil: Cisternas, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Georgia State University; Estados Unidos
Fil: Cortes, Laura R.. Georgia State University; Estados Unidos
Fil: Bruggeman, Emily C.. Emory University School Of Medicine; Estados Unidos
Fil: Yao, Bing. Emory University School Of Medicine; Estados Unidos
Fil: Forger, Nancy G.. Georgia State University; Estados Unidos - Materia
-
5-HYDROXYMETHYLCYTOSINE
5-METHYLCYTOSINE
DNA HYDROXYMETHYLATION
DNA METHYLATION
DNA METHYLTRANSFERASES
HIPPOCAMPUS
HYPOTHALAMUS
TET METHYLCYTOSINE DIOXYGENASES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/140248
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brainCisternas, Carla DanielaCortes, Laura R.Bruggeman, Emily C.Yao, BingForger, Nancy G.5-HYDROXYMETHYLCYTOSINE5-METHYLCYTOSINEDNA HYDROXYMETHYLATIONDNA METHYLATIONDNA METHYLTRANSFERASESHIPPOCAMPUSHYPOTHALAMUSTET METHYLCYTOSINE DIOXYGENASEShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3DNA methylation is dynamically modulated during postnatal brain development, and plays a key role in neuronal lineage commitment. This epigenetic mark has also recently been implicated in the development of neural sex differences, many of which are found in the hypothalamus. The level of DNA methylation depends on a balance between the placement of methyl marks by DNA methyltransferases (Dnmts) and their removal, which is catalyzed by ten-eleven translocation (Tet) methylcytosine dioxygenases. Here, we examined developmental changes and sex differences in the expression of Tet and Dnmt enzymes from birth to adulthood in two hypothalamic regions (the preoptic area and ventromedial nucleus) and the hippocampus of mice. We found highest expression of all Tet enzymes (Tet1, Tet2, Tet3) and Dnmts (Dnmt1, Dnmt3a, Dnmt3b) in newborns, despite the fact that global methylation and hydroxymethylation were at their lowest levels at birth. Expression of the Dnmt co-activator, Dnmt3l, followed a pattern opposite to that of the canonical Dnmts (i.e., was very low in newborns and increased with age). Tet enzyme activity was much higher at birth than at weaning in both the hypothalamus and hippocampus, mirroring developmental changes in gene expression. Sex differences in Tet enzyme expression were seen in all brain regions examined during the first week of life, whereas Dnmt expression was more balanced between the sexes. Neonatal testosterone treatment of females only partially masculinized enzyme expression. Thus, Tet expression and activity are elevated during neonatal brain development, and may play important roles in sexual differentiation of the brain.Fil: Cisternas, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Georgia State University; Estados UnidosFil: Cortes, Laura R.. Georgia State University; Estados UnidosFil: Bruggeman, Emily C.. Emory University School Of Medicine; Estados UnidosFil: Yao, Bing. Emory University School Of Medicine; Estados UnidosFil: Forger, Nancy G.. Georgia State University; Estados UnidosLandes Bioscience2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140248Cisternas, Carla Daniela; Cortes, Laura R.; Bruggeman, Emily C.; Yao, Bing; Forger, Nancy G.; Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain; Landes Bioscience; Epigenetics; 15; 1-2; 8-2019; 72-841559-2294CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/15592294.2019.1649528info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15592294.2019.1649528info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:09Zoai:ri.conicet.gov.ar:11336/140248instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:10.235CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain |
title |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain |
spellingShingle |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain Cisternas, Carla Daniela 5-HYDROXYMETHYLCYTOSINE 5-METHYLCYTOSINE DNA HYDROXYMETHYLATION DNA METHYLATION DNA METHYLTRANSFERASES HIPPOCAMPUS HYPOTHALAMUS TET METHYLCYTOSINE DIOXYGENASES |
title_short |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain |
title_full |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain |
title_fullStr |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain |
title_full_unstemmed |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain |
title_sort |
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain |
dc.creator.none.fl_str_mv |
Cisternas, Carla Daniela Cortes, Laura R. Bruggeman, Emily C. Yao, Bing Forger, Nancy G. |
author |
Cisternas, Carla Daniela |
author_facet |
Cisternas, Carla Daniela Cortes, Laura R. Bruggeman, Emily C. Yao, Bing Forger, Nancy G. |
author_role |
author |
author2 |
Cortes, Laura R. Bruggeman, Emily C. Yao, Bing Forger, Nancy G. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
5-HYDROXYMETHYLCYTOSINE 5-METHYLCYTOSINE DNA HYDROXYMETHYLATION DNA METHYLATION DNA METHYLTRANSFERASES HIPPOCAMPUS HYPOTHALAMUS TET METHYLCYTOSINE DIOXYGENASES |
topic |
5-HYDROXYMETHYLCYTOSINE 5-METHYLCYTOSINE DNA HYDROXYMETHYLATION DNA METHYLATION DNA METHYLTRANSFERASES HIPPOCAMPUS HYPOTHALAMUS TET METHYLCYTOSINE DIOXYGENASES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
DNA methylation is dynamically modulated during postnatal brain development, and plays a key role in neuronal lineage commitment. This epigenetic mark has also recently been implicated in the development of neural sex differences, many of which are found in the hypothalamus. The level of DNA methylation depends on a balance between the placement of methyl marks by DNA methyltransferases (Dnmts) and their removal, which is catalyzed by ten-eleven translocation (Tet) methylcytosine dioxygenases. Here, we examined developmental changes and sex differences in the expression of Tet and Dnmt enzymes from birth to adulthood in two hypothalamic regions (the preoptic area and ventromedial nucleus) and the hippocampus of mice. We found highest expression of all Tet enzymes (Tet1, Tet2, Tet3) and Dnmts (Dnmt1, Dnmt3a, Dnmt3b) in newborns, despite the fact that global methylation and hydroxymethylation were at their lowest levels at birth. Expression of the Dnmt co-activator, Dnmt3l, followed a pattern opposite to that of the canonical Dnmts (i.e., was very low in newborns and increased with age). Tet enzyme activity was much higher at birth than at weaning in both the hypothalamus and hippocampus, mirroring developmental changes in gene expression. Sex differences in Tet enzyme expression were seen in all brain regions examined during the first week of life, whereas Dnmt expression was more balanced between the sexes. Neonatal testosterone treatment of females only partially masculinized enzyme expression. Thus, Tet expression and activity are elevated during neonatal brain development, and may play important roles in sexual differentiation of the brain. Fil: Cisternas, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Georgia State University; Estados Unidos Fil: Cortes, Laura R.. Georgia State University; Estados Unidos Fil: Bruggeman, Emily C.. Emory University School Of Medicine; Estados Unidos Fil: Yao, Bing. Emory University School Of Medicine; Estados Unidos Fil: Forger, Nancy G.. Georgia State University; Estados Unidos |
description |
DNA methylation is dynamically modulated during postnatal brain development, and plays a key role in neuronal lineage commitment. This epigenetic mark has also recently been implicated in the development of neural sex differences, many of which are found in the hypothalamus. The level of DNA methylation depends on a balance between the placement of methyl marks by DNA methyltransferases (Dnmts) and their removal, which is catalyzed by ten-eleven translocation (Tet) methylcytosine dioxygenases. Here, we examined developmental changes and sex differences in the expression of Tet and Dnmt enzymes from birth to adulthood in two hypothalamic regions (the preoptic area and ventromedial nucleus) and the hippocampus of mice. We found highest expression of all Tet enzymes (Tet1, Tet2, Tet3) and Dnmts (Dnmt1, Dnmt3a, Dnmt3b) in newborns, despite the fact that global methylation and hydroxymethylation were at their lowest levels at birth. Expression of the Dnmt co-activator, Dnmt3l, followed a pattern opposite to that of the canonical Dnmts (i.e., was very low in newborns and increased with age). Tet enzyme activity was much higher at birth than at weaning in both the hypothalamus and hippocampus, mirroring developmental changes in gene expression. Sex differences in Tet enzyme expression were seen in all brain regions examined during the first week of life, whereas Dnmt expression was more balanced between the sexes. Neonatal testosterone treatment of females only partially masculinized enzyme expression. Thus, Tet expression and activity are elevated during neonatal brain development, and may play important roles in sexual differentiation of the brain. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/140248 Cisternas, Carla Daniela; Cortes, Laura R.; Bruggeman, Emily C.; Yao, Bing; Forger, Nancy G.; Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain; Landes Bioscience; Epigenetics; 15; 1-2; 8-2019; 72-84 1559-2294 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/140248 |
identifier_str_mv |
Cisternas, Carla Daniela; Cortes, Laura R.; Bruggeman, Emily C.; Yao, Bing; Forger, Nancy G.; Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain; Landes Bioscience; Epigenetics; 15; 1-2; 8-2019; 72-84 1559-2294 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1080/15592294.2019.1649528 info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15592294.2019.1649528 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Landes Bioscience |
publisher.none.fl_str_mv |
Landes Bioscience |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614121946873856 |
score |
13.070432 |