Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples

Autores
Contin, Mario Daniel; Flor, Sabrina Andrea; Lucangioli, Silvia Edith; Tripodi, Valeria Paula
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An estrogen selected molecular imprinted polymer (MIP) has been developed to be used as an alternative sorbent in solid phase extraction of serum samples before capillary electrophoresis analysis. Following a heat polymerization, MIP has been synthesized using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker, benzoyl peroxide as radical initiator and 17β- estradiol as template, and toluene as porogen. The optimized molecular imprinted solid phase extraction (MISPE) was compared to traditional C18 solid phase extraction to purify estrogens from complex matrices. It was concluded that the MISPE system developed is useful for the selective extraction of 17β- estradiol and its major natural related estrogens, estriol and estrone, from serum samples with minimal interferences and high recoveries.
Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
Estrogens
Molecular Imprinting
Solid Phase Extraction
Serum
Capillary Electrophoresis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30526
Acceder
id CONICETDig_bbe99fddbf0ab6447422e50850116e90
oai_identifier_str oai:ri.conicet.gov.ar:11336/30526
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum SamplesContin, Mario DanielFlor, Sabrina AndreaLucangioli, Silvia EdithTripodi, Valeria PaulaEstrogensMolecular ImprintingSolid Phase ExtractionSerumCapillary Electrophoresishttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3An estrogen selected molecular imprinted polymer (MIP) has been developed to be used as an alternative sorbent in solid phase extraction of serum samples before capillary electrophoresis analysis. Following a heat polymerization, MIP has been synthesized using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker, benzoyl peroxide as radical initiator and 17β- estradiol as template, and toluene as porogen. The optimized molecular imprinted solid phase extraction (MISPE) was compared to traditional C18 solid phase extraction to purify estrogens from complex matrices. It was concluded that the MISPE system developed is useful for the selective extraction of 17β- estradiol and its major natural related estrogens, estriol and estrone, from serum samples with minimal interferences and high recoveries.Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaBentham Science Publishers2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30526Contin, Mario Daniel; Flor, Sabrina Andrea; Lucangioli, Silvia Edith; Tripodi, Valeria Paula; Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples; Bentham Science Publishers; Current Analytical Chemistry; 10; 2; 12-2014; 235-2401573-41101875-6727CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/111746info:eu-repo/semantics/altIdentifier/doi/10.2174/15734110113090990027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:02Zoai:ri.conicet.gov.ar:11336/30526instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:02.308CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples
title Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples
spellingShingle Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples
Contin, Mario Daniel
Estrogens
Molecular Imprinting
Solid Phase Extraction
Serum
Capillary Electrophoresis
title_short Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples
title_full Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples
title_fullStr Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples
title_full_unstemmed Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples
title_sort Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples
dc.creator.none.fl_str_mv Contin, Mario Daniel
Flor, Sabrina Andrea
Lucangioli, Silvia Edith
Tripodi, Valeria Paula
author Contin, Mario Daniel
author_facet Contin, Mario Daniel
Flor, Sabrina Andrea
Lucangioli, Silvia Edith
Tripodi, Valeria Paula
author_role author
author2 Flor, Sabrina Andrea
Lucangioli, Silvia Edith
Tripodi, Valeria Paula
author2_role author
author
author
dc.subject.none.fl_str_mv Estrogens
Molecular Imprinting
Solid Phase Extraction
Serum
Capillary Electrophoresis
topic Estrogens
Molecular Imprinting
Solid Phase Extraction
Serum
Capillary Electrophoresis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv An estrogen selected molecular imprinted polymer (MIP) has been developed to be used as an alternative sorbent in solid phase extraction of serum samples before capillary electrophoresis analysis. Following a heat polymerization, MIP has been synthesized using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker, benzoyl peroxide as radical initiator and 17β- estradiol as template, and toluene as porogen. The optimized molecular imprinted solid phase extraction (MISPE) was compared to traditional C18 solid phase extraction to purify estrogens from complex matrices. It was concluded that the MISPE system developed is useful for the selective extraction of 17β- estradiol and its major natural related estrogens, estriol and estrone, from serum samples with minimal interferences and high recoveries.
Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description An estrogen selected molecular imprinted polymer (MIP) has been developed to be used as an alternative sorbent in solid phase extraction of serum samples before capillary electrophoresis analysis. Following a heat polymerization, MIP has been synthesized using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker, benzoyl peroxide as radical initiator and 17β- estradiol as template, and toluene as porogen. The optimized molecular imprinted solid phase extraction (MISPE) was compared to traditional C18 solid phase extraction to purify estrogens from complex matrices. It was concluded that the MISPE system developed is useful for the selective extraction of 17β- estradiol and its major natural related estrogens, estriol and estrone, from serum samples with minimal interferences and high recoveries.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30526
Contin, Mario Daniel; Flor, Sabrina Andrea; Lucangioli, Silvia Edith; Tripodi, Valeria Paula; Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples; Bentham Science Publishers; Current Analytical Chemistry; 10; 2; 12-2014; 235-240
1573-4110
1875-6727
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30526
identifier_str_mv Contin, Mario Daniel; Flor, Sabrina Andrea; Lucangioli, Silvia Edith; Tripodi, Valeria Paula; Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples; Bentham Science Publishers; Current Analytical Chemistry; 10; 2; 12-2014; 235-240
1573-4110
1875-6727
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/111746
info:eu-repo/semantics/altIdentifier/doi/10.2174/15734110113090990027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268898339061760
score 13.13397

Publicaciones similares