Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions

Autores
Gitman, Iván Federico Berco; Martinez Moyano, Edgar; Grossi, Cecilia Eugenia María; Ulloa, Rita Maria
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Analysis of the potato genome (SPUD DB) allowed us to identify 142 protease inhibitors (PIs); 42 PI genes are clustered in tandem in chr.3, position 43 Mbp (3.43): 14 encode Kunitz-type PIs (KTIs), 4 encode trypsin type PIs (TPI) and 1 encodes a cystein PI (CPI), and at position 48 MBp (3.48), 10 genes encode KTIs, 8 encode PIN-II and 5 encode serine type PI (PIN-I). On the other hand, 11 genes encoding 5 KTIs, 3 cystatin B, and 3 PIN-I are grouped in Chr. 6, and 12 PIN-I genes are in chr. 9. This multigene family regulates protein turn-over preventing catabolism of essential proteins during metabolic processes and plays a role in the defense against heterologous proteases from pathogens and pests. PIs have been proposed as an alternative to chemical pesticides for the control of herbivorous insects, and as abiotic stress-protective factors. Degenerate primers were designed against five PIs (Soltu.DM.03G018480, -G018520, -G018580, - G018620, -G018650) of 3.43, seven PIs (Soltu.DM.03G023490, -G023500, -G23510, -G23520, -G23530, - G23540, and -G024110) of 3.48, and seven PIs of Chr. 9 (Soltu.DM.09G025840, -G025850 -G025860 - G025880, -G025900, -G025910, and -G025930) that share at least 83.46, 55,2 and 77,53 % of nucleotide identity. According to RNAseq data these three gene clusters are induced upon drought, salt, mannitol and N2 fertilization, and are downregulated during tuber dormancy release. To confirm the RNAseq data, RT-qPCR assays were performed using RNAs from in-vitro plants cultured under control conditions or a) with different NH4+ and NO3- concentrations, b) exposed during 1 week to continuous darkness, and c) to 150 mM NaCl during 24 and 96 h. In addition, RNA was extracted from greenhouse plants exposed to drought conditions. To analyze, the role of PIs during source-sink processes, RNA was obtained from dormant and sprouting tubers, from etiolated and green sprouts, and from young, fully developed and senescent leaves. In the future we aim to characterize one gene of each cluster in overexpressing or knockdown plants to elucidate their potential as biotechnological tools.
Fil: Gitman, Iván Federico Berco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Martinez Moyano, Edgar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Grossi, Cecilia Eugenia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Mendoza
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
PROTEASE INHIBITORS
POTATO
STRESS RESPONSE
BIOTECHNOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240813
Acceder
id CONICETDig_bb91bcb02a856f357e53dbce0c6463dc
oai_identifier_str oai:ri.conicet.gov.ar:11336/240813
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditionsGitman, Iván Federico BercoMartinez Moyano, EdgarGrossi, Cecilia Eugenia MaríaUlloa, Rita MariaPROTEASE INHIBITORSPOTATOSTRESS RESPONSEBIOTECHNOLOGYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Analysis of the potato genome (SPUD DB) allowed us to identify 142 protease inhibitors (PIs); 42 PI genes are clustered in tandem in chr.3, position 43 Mbp (3.43): 14 encode Kunitz-type PIs (KTIs), 4 encode trypsin type PIs (TPI) and 1 encodes a cystein PI (CPI), and at position 48 MBp (3.48), 10 genes encode KTIs, 8 encode PIN-II and 5 encode serine type PI (PIN-I). On the other hand, 11 genes encoding 5 KTIs, 3 cystatin B, and 3 PIN-I are grouped in Chr. 6, and 12 PIN-I genes are in chr. 9. This multigene family regulates protein turn-over preventing catabolism of essential proteins during metabolic processes and plays a role in the defense against heterologous proteases from pathogens and pests. PIs have been proposed as an alternative to chemical pesticides for the control of herbivorous insects, and as abiotic stress-protective factors. Degenerate primers were designed against five PIs (Soltu.DM.03G018480, -G018520, -G018580, - G018620, -G018650) of 3.43, seven PIs (Soltu.DM.03G023490, -G023500, -G23510, -G23520, -G23530, - G23540, and -G024110) of 3.48, and seven PIs of Chr. 9 (Soltu.DM.09G025840, -G025850 -G025860 - G025880, -G025900, -G025910, and -G025930) that share at least 83.46, 55,2 and 77,53 % of nucleotide identity. According to RNAseq data these three gene clusters are induced upon drought, salt, mannitol and N2 fertilization, and are downregulated during tuber dormancy release. To confirm the RNAseq data, RT-qPCR assays were performed using RNAs from in-vitro plants cultured under control conditions or a) with different NH4+ and NO3- concentrations, b) exposed during 1 week to continuous darkness, and c) to 150 mM NaCl during 24 and 96 h. In addition, RNA was extracted from greenhouse plants exposed to drought conditions. To analyze, the role of PIs during source-sink processes, RNA was obtained from dormant and sprouting tubers, from etiolated and green sprouts, and from young, fully developed and senescent leaves. In the future we aim to characterize one gene of each cluster in overexpressing or knockdown plants to elucidate their potential as biotechnological tools.Fil: Gitman, Iván Federico Berco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Martinez Moyano, Edgar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Grossi, Cecilia Eugenia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaLVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchMendozaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularTech Science Press2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240813Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 1-10327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:01:13Zoai:ri.conicet.gov.ar:11336/240813instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:01:14.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
title Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
spellingShingle Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
Gitman, Iván Federico Berco
PROTEASE INHIBITORS
POTATO
STRESS RESPONSE
BIOTECHNOLOGY
title_short Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
title_full Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
title_fullStr Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
title_full_unstemmed Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
title_sort Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
dc.creator.none.fl_str_mv Gitman, Iván Federico Berco
Martinez Moyano, Edgar
Grossi, Cecilia Eugenia María
Ulloa, Rita Maria
author Gitman, Iván Federico Berco
author_facet Gitman, Iván Federico Berco
Martinez Moyano, Edgar
Grossi, Cecilia Eugenia María
Ulloa, Rita Maria
author_role author
author2 Martinez Moyano, Edgar
Grossi, Cecilia Eugenia María
Ulloa, Rita Maria
author2_role author
author
author
dc.subject.none.fl_str_mv PROTEASE INHIBITORS
POTATO
STRESS RESPONSE
BIOTECHNOLOGY
topic PROTEASE INHIBITORS
POTATO
STRESS RESPONSE
BIOTECHNOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Analysis of the potato genome (SPUD DB) allowed us to identify 142 protease inhibitors (PIs); 42 PI genes are clustered in tandem in chr.3, position 43 Mbp (3.43): 14 encode Kunitz-type PIs (KTIs), 4 encode trypsin type PIs (TPI) and 1 encodes a cystein PI (CPI), and at position 48 MBp (3.48), 10 genes encode KTIs, 8 encode PIN-II and 5 encode serine type PI (PIN-I). On the other hand, 11 genes encoding 5 KTIs, 3 cystatin B, and 3 PIN-I are grouped in Chr. 6, and 12 PIN-I genes are in chr. 9. This multigene family regulates protein turn-over preventing catabolism of essential proteins during metabolic processes and plays a role in the defense against heterologous proteases from pathogens and pests. PIs have been proposed as an alternative to chemical pesticides for the control of herbivorous insects, and as abiotic stress-protective factors. Degenerate primers were designed against five PIs (Soltu.DM.03G018480, -G018520, -G018580, - G018620, -G018650) of 3.43, seven PIs (Soltu.DM.03G023490, -G023500, -G23510, -G23520, -G23530, - G23540, and -G024110) of 3.48, and seven PIs of Chr. 9 (Soltu.DM.09G025840, -G025850 -G025860 - G025880, -G025900, -G025910, and -G025930) that share at least 83.46, 55,2 and 77,53 % of nucleotide identity. According to RNAseq data these three gene clusters are induced upon drought, salt, mannitol and N2 fertilization, and are downregulated during tuber dormancy release. To confirm the RNAseq data, RT-qPCR assays were performed using RNAs from in-vitro plants cultured under control conditions or a) with different NH4+ and NO3- concentrations, b) exposed during 1 week to continuous darkness, and c) to 150 mM NaCl during 24 and 96 h. In addition, RNA was extracted from greenhouse plants exposed to drought conditions. To analyze, the role of PIs during source-sink processes, RNA was obtained from dormant and sprouting tubers, from etiolated and green sprouts, and from young, fully developed and senescent leaves. In the future we aim to characterize one gene of each cluster in overexpressing or knockdown plants to elucidate their potential as biotechnological tools.
Fil: Gitman, Iván Federico Berco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Martinez Moyano, Edgar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Grossi, Cecilia Eugenia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Mendoza
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description Analysis of the potato genome (SPUD DB) allowed us to identify 142 protease inhibitors (PIs); 42 PI genes are clustered in tandem in chr.3, position 43 Mbp (3.43): 14 encode Kunitz-type PIs (KTIs), 4 encode trypsin type PIs (TPI) and 1 encodes a cystein PI (CPI), and at position 48 MBp (3.48), 10 genes encode KTIs, 8 encode PIN-II and 5 encode serine type PI (PIN-I). On the other hand, 11 genes encoding 5 KTIs, 3 cystatin B, and 3 PIN-I are grouped in Chr. 6, and 12 PIN-I genes are in chr. 9. This multigene family regulates protein turn-over preventing catabolism of essential proteins during metabolic processes and plays a role in the defense against heterologous proteases from pathogens and pests. PIs have been proposed as an alternative to chemical pesticides for the control of herbivorous insects, and as abiotic stress-protective factors. Degenerate primers were designed against five PIs (Soltu.DM.03G018480, -G018520, -G018580, - G018620, -G018650) of 3.43, seven PIs (Soltu.DM.03G023490, -G023500, -G23510, -G23520, -G23530, - G23540, and -G024110) of 3.48, and seven PIs of Chr. 9 (Soltu.DM.09G025840, -G025850 -G025860 - G025880, -G025900, -G025910, and -G025930) that share at least 83.46, 55,2 and 77,53 % of nucleotide identity. According to RNAseq data these three gene clusters are induced upon drought, salt, mannitol and N2 fertilization, and are downregulated during tuber dormancy release. To confirm the RNAseq data, RT-qPCR assays were performed using RNAs from in-vitro plants cultured under control conditions or a) with different NH4+ and NO3- concentrations, b) exposed during 1 week to continuous darkness, and c) to 150 mM NaCl during 24 and 96 h. In addition, RNA was extracted from greenhouse plants exposed to drought conditions. To analyze, the role of PIs during source-sink processes, RNA was obtained from dormant and sprouting tubers, from etiolated and green sprouts, and from young, fully developed and senescent leaves. In the future we aim to characterize one gene of each cluster in overexpressing or knockdown plants to elucidate their potential as biotechnological tools.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240813
Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 1-1
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240813
identifier_str_mv Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 1-1
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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