Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles

Autores
Attademo, Andres Maximiliano; Curi, Lucila Marilén; Cuzziol Boccioni, Ana Paula; Barrios, Carlos Eduardo; Peltzer, Paola; Simoniello, María F.; Lajmanovich, Rafael Carlos; Michlig, Melina Paola; Repetti, María Rosa; Rios, Juan Manuel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyethylene microplastics (PE-MPs), a whitish thermoplastic polymer with numerous applications, is one of the materials most widely used in the industrial sector, whereas tetrabromobisphenol A (TBBPA) is a brominated flame retardant. The aim of this study was to analyze the effects of PE-MPs and TBBPA on Rhinella arenarum tadpoles at the laboratory scale. Tadpoles were chronically exposed (30 days) to four treatments: PE-MPs (60 mg L−1), TBBPA (10 µg L−1), their mixture (PE-MPs + TBBPA), and dechlorinated water as negative control (CO). Biomarkers of enzymatic activity (acetylcholinesterase, AChE; carboxylesterase, CbE; glutathione reductase, GR; and glutathione-S-transferase, GST), hepatic physiological alteration (alkaline phosphatase; ALP activity, and cholesterol; CHOL level), and endocrine disruption through thyroid hormone (T4) levels were assessed. In addition, intestine and liver were histomorphologically evaluated. AChE activity in tadpoles was significantly inhibited after exposure to PE-MPs and TBBPA with respect to CO. In addition, CbE, GR, and ALP activities showed higher values in the mixture of PE-MPs+ TBBPA treatment than in CO, whereas CHOL level was higher in TBBPA and PE-MPs+ TBBPA treatments than in CO. GST activity did not show significant differences between treatments and CO. T4 levels increased significantly in all treatments with respect to CO. The intestinal structure of tadpoles exposed to PE-MPs and PE-MPs+ TBBPA showed signs of mechanical damage. The intestinal wall of tadpoles under PE-MPs, TBBPA and PE-MPs+ TBBPA treatments was thicker than that of CO individuals. The analysis of liver histology demonstrated the hepatotoxicity caused by PE-MPs+ TBBPA. This study provides quantitative evidence of the harmful effects of PE-MPs, TBBPA and their mixture on enzymatic and hormonal activities, and histological evidence of intestinal wall hypertrophy and liver damage of R. arenarum tadpoles.
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Curi, Lucila Marilén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Cuzziol Boccioni, Ana Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Barrios, Carlos Eduardo. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Simoniello, María F.. Universidad Nacional del Litoral; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Michlig, Melina Paola. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Nordeste; Argentina
Fil: Rios, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
ANURAN TADPOLES
BIOMARKERS
POLYETHYLENE MICROPLASTICS
TBBPA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/224590
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/224590
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpolesAttademo, Andres MaximilianoCuri, Lucila MarilénCuzziol Boccioni, Ana PaulaBarrios, Carlos EduardoPeltzer, PaolaSimoniello, María F.Lajmanovich, Rafael CarlosMichlig, Melina PaolaRepetti, María RosaRios, Juan ManuelANURAN TADPOLESBIOMARKERSPOLYETHYLENE MICROPLASTICSTBBPAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Polyethylene microplastics (PE-MPs), a whitish thermoplastic polymer with numerous applications, is one of the materials most widely used in the industrial sector, whereas tetrabromobisphenol A (TBBPA) is a brominated flame retardant. The aim of this study was to analyze the effects of PE-MPs and TBBPA on Rhinella arenarum tadpoles at the laboratory scale. Tadpoles were chronically exposed (30 days) to four treatments: PE-MPs (60 mg L−1), TBBPA (10 µg L−1), their mixture (PE-MPs + TBBPA), and dechlorinated water as negative control (CO). Biomarkers of enzymatic activity (acetylcholinesterase, AChE; carboxylesterase, CbE; glutathione reductase, GR; and glutathione-S-transferase, GST), hepatic physiological alteration (alkaline phosphatase; ALP activity, and cholesterol; CHOL level), and endocrine disruption through thyroid hormone (T4) levels were assessed. In addition, intestine and liver were histomorphologically evaluated. AChE activity in tadpoles was significantly inhibited after exposure to PE-MPs and TBBPA with respect to CO. In addition, CbE, GR, and ALP activities showed higher values in the mixture of PE-MPs+ TBBPA treatment than in CO, whereas CHOL level was higher in TBBPA and PE-MPs+ TBBPA treatments than in CO. GST activity did not show significant differences between treatments and CO. T4 levels increased significantly in all treatments with respect to CO. The intestinal structure of tadpoles exposed to PE-MPs and PE-MPs+ TBBPA showed signs of mechanical damage. The intestinal wall of tadpoles under PE-MPs, TBBPA and PE-MPs+ TBBPA treatments was thicker than that of CO individuals. The analysis of liver histology demonstrated the hepatotoxicity caused by PE-MPs+ TBBPA. This study provides quantitative evidence of the harmful effects of PE-MPs, TBBPA and their mixture on enzymatic and hormonal activities, and histological evidence of intestinal wall hypertrophy and liver damage of R. arenarum tadpoles.Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Curi, Lucila Marilén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Cuzziol Boccioni, Ana Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Barrios, Carlos Eduardo. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Peltzer, Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Simoniello, María F.. Universidad Nacional del Litoral; ArgentinaFil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Michlig, Melina Paola. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Nordeste; ArgentinaFil: Rios, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaElsevier2023-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/224590Attademo, Andres Maximiliano; Curi, Lucila Marilén; Cuzziol Boccioni, Ana Paula; Barrios, Carlos Eduardo; Peltzer, Paola; et al.; Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles; Elsevier; Environmental Advances; 14; 12-2023; 1-432666-7657CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2666765723001023info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envadv.2023.100444info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:45Zoai:ri.conicet.gov.ar:11336/224590instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:45.985CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles
title Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles
spellingShingle Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles
Attademo, Andres Maximiliano
ANURAN TADPOLES
BIOMARKERS
POLYETHYLENE MICROPLASTICS
TBBPA
title_short Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles
title_full Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles
title_fullStr Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles
title_full_unstemmed Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles
title_sort Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles
dc.creator.none.fl_str_mv Attademo, Andres Maximiliano
Curi, Lucila Marilén
Cuzziol Boccioni, Ana Paula
Barrios, Carlos Eduardo
Peltzer, Paola
Simoniello, María F.
Lajmanovich, Rafael Carlos
Michlig, Melina Paola
Repetti, María Rosa
Rios, Juan Manuel
author Attademo, Andres Maximiliano
author_facet Attademo, Andres Maximiliano
Curi, Lucila Marilén
Cuzziol Boccioni, Ana Paula
Barrios, Carlos Eduardo
Peltzer, Paola
Simoniello, María F.
Lajmanovich, Rafael Carlos
Michlig, Melina Paola
Repetti, María Rosa
Rios, Juan Manuel
author_role author
author2 Curi, Lucila Marilén
Cuzziol Boccioni, Ana Paula
Barrios, Carlos Eduardo
Peltzer, Paola
Simoniello, María F.
Lajmanovich, Rafael Carlos
Michlig, Melina Paola
Repetti, María Rosa
Rios, Juan Manuel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANURAN TADPOLES
BIOMARKERS
POLYETHYLENE MICROPLASTICS
TBBPA
topic ANURAN TADPOLES
BIOMARKERS
POLYETHYLENE MICROPLASTICS
TBBPA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polyethylene microplastics (PE-MPs), a whitish thermoplastic polymer with numerous applications, is one of the materials most widely used in the industrial sector, whereas tetrabromobisphenol A (TBBPA) is a brominated flame retardant. The aim of this study was to analyze the effects of PE-MPs and TBBPA on Rhinella arenarum tadpoles at the laboratory scale. Tadpoles were chronically exposed (30 days) to four treatments: PE-MPs (60 mg L−1), TBBPA (10 µg L−1), their mixture (PE-MPs + TBBPA), and dechlorinated water as negative control (CO). Biomarkers of enzymatic activity (acetylcholinesterase, AChE; carboxylesterase, CbE; glutathione reductase, GR; and glutathione-S-transferase, GST), hepatic physiological alteration (alkaline phosphatase; ALP activity, and cholesterol; CHOL level), and endocrine disruption through thyroid hormone (T4) levels were assessed. In addition, intestine and liver were histomorphologically evaluated. AChE activity in tadpoles was significantly inhibited after exposure to PE-MPs and TBBPA with respect to CO. In addition, CbE, GR, and ALP activities showed higher values in the mixture of PE-MPs+ TBBPA treatment than in CO, whereas CHOL level was higher in TBBPA and PE-MPs+ TBBPA treatments than in CO. GST activity did not show significant differences between treatments and CO. T4 levels increased significantly in all treatments with respect to CO. The intestinal structure of tadpoles exposed to PE-MPs and PE-MPs+ TBBPA showed signs of mechanical damage. The intestinal wall of tadpoles under PE-MPs, TBBPA and PE-MPs+ TBBPA treatments was thicker than that of CO individuals. The analysis of liver histology demonstrated the hepatotoxicity caused by PE-MPs+ TBBPA. This study provides quantitative evidence of the harmful effects of PE-MPs, TBBPA and their mixture on enzymatic and hormonal activities, and histological evidence of intestinal wall hypertrophy and liver damage of R. arenarum tadpoles.
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Curi, Lucila Marilén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Cuzziol Boccioni, Ana Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Barrios, Carlos Eduardo. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Simoniello, María F.. Universidad Nacional del Litoral; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Michlig, Melina Paola. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Nordeste; Argentina
Fil: Rios, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description Polyethylene microplastics (PE-MPs), a whitish thermoplastic polymer with numerous applications, is one of the materials most widely used in the industrial sector, whereas tetrabromobisphenol A (TBBPA) is a brominated flame retardant. The aim of this study was to analyze the effects of PE-MPs and TBBPA on Rhinella arenarum tadpoles at the laboratory scale. Tadpoles were chronically exposed (30 days) to four treatments: PE-MPs (60 mg L−1), TBBPA (10 µg L−1), their mixture (PE-MPs + TBBPA), and dechlorinated water as negative control (CO). Biomarkers of enzymatic activity (acetylcholinesterase, AChE; carboxylesterase, CbE; glutathione reductase, GR; and glutathione-S-transferase, GST), hepatic physiological alteration (alkaline phosphatase; ALP activity, and cholesterol; CHOL level), and endocrine disruption through thyroid hormone (T4) levels were assessed. In addition, intestine and liver were histomorphologically evaluated. AChE activity in tadpoles was significantly inhibited after exposure to PE-MPs and TBBPA with respect to CO. In addition, CbE, GR, and ALP activities showed higher values in the mixture of PE-MPs+ TBBPA treatment than in CO, whereas CHOL level was higher in TBBPA and PE-MPs+ TBBPA treatments than in CO. GST activity did not show significant differences between treatments and CO. T4 levels increased significantly in all treatments with respect to CO. The intestinal structure of tadpoles exposed to PE-MPs and PE-MPs+ TBBPA showed signs of mechanical damage. The intestinal wall of tadpoles under PE-MPs, TBBPA and PE-MPs+ TBBPA treatments was thicker than that of CO individuals. The analysis of liver histology demonstrated the hepatotoxicity caused by PE-MPs+ TBBPA. This study provides quantitative evidence of the harmful effects of PE-MPs, TBBPA and their mixture on enzymatic and hormonal activities, and histological evidence of intestinal wall hypertrophy and liver damage of R. arenarum tadpoles.
publishDate 2023
dc.date.none.fl_str_mv 2023-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/224590
Attademo, Andres Maximiliano; Curi, Lucila Marilén; Cuzziol Boccioni, Ana Paula; Barrios, Carlos Eduardo; Peltzer, Paola; et al.; Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles; Elsevier; Environmental Advances; 14; 12-2023; 1-43
2666-7657
CONICET Digital
CONICET
url http://hdl.handle.net/11336/224590
identifier_str_mv Attademo, Andres Maximiliano; Curi, Lucila Marilén; Cuzziol Boccioni, Ana Paula; Barrios, Carlos Eduardo; Peltzer, Paola; et al.; Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles; Elsevier; Environmental Advances; 14; 12-2023; 1-43
2666-7657
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2666765723001023
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envadv.2023.100444
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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