When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?

Autores
Soldi, Natalia; Ferella, Luciana; Schinder, Alejandro Fabián; Piatti, Veronica del Carmen
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Our mammalian GPS, Global Positioning System, is made of diverse brain structures such as the dentate gyrus (DG) of the hippocampus. This is one of the most plastic brain regions because it has adult neurogenesis and this process is modulated by the animal experiences.The most accepted function of the DG and its new neurons is the discrimination of similar spatial contexts. However, their contribution in an ordinary behaviour remains unknown. We started investigating the hypothesis that the DG would be necessary to solve difficult spatial challenges but not easy ones in a goal guided behaviour. Therefore, adult mice were infected with AAV flex-hM4Di plus CAG-Cre retrovirus in the DG to allow chemogenetic manipulation of neuronal activity in mice navigating in a crossword maze after the i. p. injection of the synthetic agonist clozapine-N-oxide (?CNO?) or its vehicle (?control?). In a single day mice had to learn two shortest paths to a new reward location. We designed spatial routes of two levels of cognitive demand and evaluated mice performance with or without chemogenetic inhibition (CNO vs. control). Easy and difficult journeys were equally solved and learnt by mice under control condition. However, mice receiving CNO could only learn easy spatial trajectories but not the difficult ones (p = 0.01, Wilcoxon signed rank test, N = 4 mice). Therefore, our preliminary data suggest that the DG is required by the GPS during spatial navigation in complex mnemonic challenges.
Fil: Soldi, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Ferella, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
CROSSWORD MAZE
hM4Di
NOVELTY
CLOZAPINE-N-OXIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/164580
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/164580
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network_name_str CONICET Digital (CONICET)
spelling When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?Soldi, NataliaFerella, LucianaSchinder, Alejandro FabiánPiatti, Veronica del CarmenCROSSWORD MAZEhM4DiNOVELTYCLOZAPINE-N-OXIDEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Our mammalian GPS, Global Positioning System, is made of diverse brain structures such as the dentate gyrus (DG) of the hippocampus. This is one of the most plastic brain regions because it has adult neurogenesis and this process is modulated by the animal experiences.The most accepted function of the DG and its new neurons is the discrimination of similar spatial contexts. However, their contribution in an ordinary behaviour remains unknown. We started investigating the hypothesis that the DG would be necessary to solve difficult spatial challenges but not easy ones in a goal guided behaviour. Therefore, adult mice were infected with AAV flex-hM4Di plus CAG-Cre retrovirus in the DG to allow chemogenetic manipulation of neuronal activity in mice navigating in a crossword maze after the i. p. injection of the synthetic agonist clozapine-N-oxide (?CNO?) or its vehicle (?control?). In a single day mice had to learn two shortest paths to a new reward location. We designed spatial routes of two levels of cognitive demand and evaluated mice performance with or without chemogenetic inhibition (CNO vs. control). Easy and difficult journeys were equally solved and learnt by mice under control condition. However, mice receiving CNO could only learn easy spatial trajectories but not the difficult ones (p = 0.01, Wilcoxon signed rank test, N = 4 mice). Therefore, our preliminary data suggest that the DG is required by the GPS during spatial navigation in complex mnemonic challenges.Fil: Soldi, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ferella, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaXXXIV Annual Meeting of the Argentine Society for Research in NeuroscienceArgentinaSociedad Argentina de Investigación en NeurocienciasSAGE Publications2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/164580When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?; XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience; Argentina; 2019; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/pdf/10.1177/1759091420979851info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/full/10.1177/1759091420979851info:eu-repo/semantics/altIdentifier/doi/10.1177/1759091420979851Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:31Zoai:ri.conicet.gov.ar:11336/164580instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:31.878CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
title When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
spellingShingle When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
Soldi, Natalia
CROSSWORD MAZE
hM4Di
NOVELTY
CLOZAPINE-N-OXIDE
title_short When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
title_full When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
title_fullStr When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
title_full_unstemmed When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
title_sort When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
dc.creator.none.fl_str_mv Soldi, Natalia
Ferella, Luciana
Schinder, Alejandro Fabián
Piatti, Veronica del Carmen
author Soldi, Natalia
author_facet Soldi, Natalia
Ferella, Luciana
Schinder, Alejandro Fabián
Piatti, Veronica del Carmen
author_role author
author2 Ferella, Luciana
Schinder, Alejandro Fabián
Piatti, Veronica del Carmen
author2_role author
author
author
dc.subject.none.fl_str_mv CROSSWORD MAZE
hM4Di
NOVELTY
CLOZAPINE-N-OXIDE
topic CROSSWORD MAZE
hM4Di
NOVELTY
CLOZAPINE-N-OXIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Our mammalian GPS, Global Positioning System, is made of diverse brain structures such as the dentate gyrus (DG) of the hippocampus. This is one of the most plastic brain regions because it has adult neurogenesis and this process is modulated by the animal experiences.The most accepted function of the DG and its new neurons is the discrimination of similar spatial contexts. However, their contribution in an ordinary behaviour remains unknown. We started investigating the hypothesis that the DG would be necessary to solve difficult spatial challenges but not easy ones in a goal guided behaviour. Therefore, adult mice were infected with AAV flex-hM4Di plus CAG-Cre retrovirus in the DG to allow chemogenetic manipulation of neuronal activity in mice navigating in a crossword maze after the i. p. injection of the synthetic agonist clozapine-N-oxide (?CNO?) or its vehicle (?control?). In a single day mice had to learn two shortest paths to a new reward location. We designed spatial routes of two levels of cognitive demand and evaluated mice performance with or without chemogenetic inhibition (CNO vs. control). Easy and difficult journeys were equally solved and learnt by mice under control condition. However, mice receiving CNO could only learn easy spatial trajectories but not the difficult ones (p = 0.01, Wilcoxon signed rank test, N = 4 mice). Therefore, our preliminary data suggest that the DG is required by the GPS during spatial navigation in complex mnemonic challenges.
Fil: Soldi, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Ferella, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience
Argentina
Sociedad Argentina de Investigación en Neurociencias
description Our mammalian GPS, Global Positioning System, is made of diverse brain structures such as the dentate gyrus (DG) of the hippocampus. This is one of the most plastic brain regions because it has adult neurogenesis and this process is modulated by the animal experiences.The most accepted function of the DG and its new neurons is the discrimination of similar spatial contexts. However, their contribution in an ordinary behaviour remains unknown. We started investigating the hypothesis that the DG would be necessary to solve difficult spatial challenges but not easy ones in a goal guided behaviour. Therefore, adult mice were infected with AAV flex-hM4Di plus CAG-Cre retrovirus in the DG to allow chemogenetic manipulation of neuronal activity in mice navigating in a crossword maze after the i. p. injection of the synthetic agonist clozapine-N-oxide (?CNO?) or its vehicle (?control?). In a single day mice had to learn two shortest paths to a new reward location. We designed spatial routes of two levels of cognitive demand and evaluated mice performance with or without chemogenetic inhibition (CNO vs. control). Easy and difficult journeys were equally solved and learnt by mice under control condition. However, mice receiving CNO could only learn easy spatial trajectories but not the difficult ones (p = 0.01, Wilcoxon signed rank test, N = 4 mice). Therefore, our preliminary data suggest that the DG is required by the GPS during spatial navigation in complex mnemonic challenges.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/164580
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?; XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience; Argentina; 2019; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/164580
identifier_str_mv When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?; XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience; Argentina; 2019; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/pdf/10.1177/1759091420979851
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/full/10.1177/1759091420979851
info:eu-repo/semantics/altIdentifier/doi/10.1177/1759091420979851
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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