When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?
- Autores
- Soldi, Natalia; Ferella, Luciana; Schinder, Alejandro Fabián; Piatti, Veronica del Carmen
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Our mammalian GPS, Global Positioning System, is made of diverse brain structures such as the dentate gyrus (DG) of the hippocampus. This is one of the most plastic brain regions because it has adult neurogenesis and this process is modulated by the animal experiences.The most accepted function of the DG and its new neurons is the discrimination of similar spatial contexts. However, their contribution in an ordinary behaviour remains unknown. We started investigating the hypothesis that the DG would be necessary to solve difficult spatial challenges but not easy ones in a goal guided behaviour. Therefore, adult mice were infected with AAV flex-hM4Di plus CAG-Cre retrovirus in the DG to allow chemogenetic manipulation of neuronal activity in mice navigating in a crossword maze after the i. p. injection of the synthetic agonist clozapine-N-oxide (?CNO?) or its vehicle (?control?). In a single day mice had to learn two shortest paths to a new reward location. We designed spatial routes of two levels of cognitive demand and evaluated mice performance with or without chemogenetic inhibition (CNO vs. control). Easy and difficult journeys were equally solved and learnt by mice under control condition. However, mice receiving CNO could only learn easy spatial trajectories but not the difficult ones (p = 0.01, Wilcoxon signed rank test, N = 4 mice). Therefore, our preliminary data suggest that the DG is required by the GPS during spatial navigation in complex mnemonic challenges.
Fil: Soldi, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Ferella, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience
Argentina
Sociedad Argentina de Investigación en Neurociencias - Materia
-
CROSSWORD MAZE
hM4Di
NOVELTY
CLOZAPINE-N-OXIDE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/164580
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When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?Soldi, NataliaFerella, LucianaSchinder, Alejandro FabiánPiatti, Veronica del CarmenCROSSWORD MAZEhM4DiNOVELTYCLOZAPINE-N-OXIDEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Our mammalian GPS, Global Positioning System, is made of diverse brain structures such as the dentate gyrus (DG) of the hippocampus. This is one of the most plastic brain regions because it has adult neurogenesis and this process is modulated by the animal experiences.The most accepted function of the DG and its new neurons is the discrimination of similar spatial contexts. However, their contribution in an ordinary behaviour remains unknown. We started investigating the hypothesis that the DG would be necessary to solve difficult spatial challenges but not easy ones in a goal guided behaviour. Therefore, adult mice were infected with AAV flex-hM4Di plus CAG-Cre retrovirus in the DG to allow chemogenetic manipulation of neuronal activity in mice navigating in a crossword maze after the i. p. injection of the synthetic agonist clozapine-N-oxide (?CNO?) or its vehicle (?control?). In a single day mice had to learn two shortest paths to a new reward location. We designed spatial routes of two levels of cognitive demand and evaluated mice performance with or without chemogenetic inhibition (CNO vs. control). Easy and difficult journeys were equally solved and learnt by mice under control condition. However, mice receiving CNO could only learn easy spatial trajectories but not the difficult ones (p = 0.01, Wilcoxon signed rank test, N = 4 mice). Therefore, our preliminary data suggest that the DG is required by the GPS during spatial navigation in complex mnemonic challenges.Fil: Soldi, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ferella, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaXXXIV Annual Meeting of the Argentine Society for Research in NeuroscienceArgentinaSociedad Argentina de Investigación en NeurocienciasSAGE Publications2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/164580When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?; XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience; Argentina; 2019; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/pdf/10.1177/1759091420979851info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/full/10.1177/1759091420979851info:eu-repo/semantics/altIdentifier/doi/10.1177/1759091420979851Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:38Zoai:ri.conicet.gov.ar:11336/164580instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:39.269CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation? |
title |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation? |
spellingShingle |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation? Soldi, Natalia CROSSWORD MAZE hM4Di NOVELTY CLOZAPINE-N-OXIDE |
title_short |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation? |
title_full |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation? |
title_fullStr |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation? |
title_full_unstemmed |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation? |
title_sort |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation? |
dc.creator.none.fl_str_mv |
Soldi, Natalia Ferella, Luciana Schinder, Alejandro Fabián Piatti, Veronica del Carmen |
author |
Soldi, Natalia |
author_facet |
Soldi, Natalia Ferella, Luciana Schinder, Alejandro Fabián Piatti, Veronica del Carmen |
author_role |
author |
author2 |
Ferella, Luciana Schinder, Alejandro Fabián Piatti, Veronica del Carmen |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
CROSSWORD MAZE hM4Di NOVELTY CLOZAPINE-N-OXIDE |
topic |
CROSSWORD MAZE hM4Di NOVELTY CLOZAPINE-N-OXIDE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Our mammalian GPS, Global Positioning System, is made of diverse brain structures such as the dentate gyrus (DG) of the hippocampus. This is one of the most plastic brain regions because it has adult neurogenesis and this process is modulated by the animal experiences.The most accepted function of the DG and its new neurons is the discrimination of similar spatial contexts. However, their contribution in an ordinary behaviour remains unknown. We started investigating the hypothesis that the DG would be necessary to solve difficult spatial challenges but not easy ones in a goal guided behaviour. Therefore, adult mice were infected with AAV flex-hM4Di plus CAG-Cre retrovirus in the DG to allow chemogenetic manipulation of neuronal activity in mice navigating in a crossword maze after the i. p. injection of the synthetic agonist clozapine-N-oxide (?CNO?) or its vehicle (?control?). In a single day mice had to learn two shortest paths to a new reward location. We designed spatial routes of two levels of cognitive demand and evaluated mice performance with or without chemogenetic inhibition (CNO vs. control). Easy and difficult journeys were equally solved and learnt by mice under control condition. However, mice receiving CNO could only learn easy spatial trajectories but not the difficult ones (p = 0.01, Wilcoxon signed rank test, N = 4 mice). Therefore, our preliminary data suggest that the DG is required by the GPS during spatial navigation in complex mnemonic challenges. Fil: Soldi, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Ferella, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience Argentina Sociedad Argentina de Investigación en Neurociencias |
description |
Our mammalian GPS, Global Positioning System, is made of diverse brain structures such as the dentate gyrus (DG) of the hippocampus. This is one of the most plastic brain regions because it has adult neurogenesis and this process is modulated by the animal experiences.The most accepted function of the DG and its new neurons is the discrimination of similar spatial contexts. However, their contribution in an ordinary behaviour remains unknown. We started investigating the hypothesis that the DG would be necessary to solve difficult spatial challenges but not easy ones in a goal guided behaviour. Therefore, adult mice were infected with AAV flex-hM4Di plus CAG-Cre retrovirus in the DG to allow chemogenetic manipulation of neuronal activity in mice navigating in a crossword maze after the i. p. injection of the synthetic agonist clozapine-N-oxide (?CNO?) or its vehicle (?control?). In a single day mice had to learn two shortest paths to a new reward location. We designed spatial routes of two levels of cognitive demand and evaluated mice performance with or without chemogenetic inhibition (CNO vs. control). Easy and difficult journeys were equally solved and learnt by mice under control condition. However, mice receiving CNO could only learn easy spatial trajectories but not the difficult ones (p = 0.01, Wilcoxon signed rank test, N = 4 mice). Therefore, our preliminary data suggest that the DG is required by the GPS during spatial navigation in complex mnemonic challenges. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
format |
conferenceObject |
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http://hdl.handle.net/11336/164580 When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?; XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience; Argentina; 2019; 1-2 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/164580 |
identifier_str_mv |
When does the mammalian GPS use the dentate gyrus in everyday challenges of spatial navigation?; XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience; Argentina; 2019; 1-2 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/pdf/10.1177/1759091420979851 info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/full/10.1177/1759091420979851 info:eu-repo/semantics/altIdentifier/doi/10.1177/1759091420979851 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Nacional |
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SAGE Publications |
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SAGE Publications |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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