Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton

Autores
Vigil, Maximiliano Angel; Picco, María Elisa; Rinaldi, Debora Eugenia; Rossi, Rolando Carlos; Rey, Osvaldo; Rossi, Juan Pablo Francisco; Ferreira Gomes, Mariela Soledad
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The Plasma Membrane Calcium ATPase (PMCA) is a calmodulin-modulated P-type ATPaseresponsible for the maintenance of low intracellular concentrations of Ca2+ in mosteukaryotic cells. Our group have previously shown that purified actin can exert a dualmodulation on the activity of Ca2+-ATPase 4b isoform (hPMCA4b): F-actin inhibits it whileshort actin oligomers may contribute to its activation. These studies had to be performedwith purified proteins given the nature of the biophysical and biochemical approachesused.On the other hand, in HEK293 human cells that overexpressed PMCA2w/b isoform, theactin depolymerization upon Cytochalasin D (CytD) treatment significantly increasedPMCA2-mediated Ca2+ extrusion and when F-actin was stabilized using jasplakinolide,PMCA2w/b activity was completely abolished.In order to assess whether the functional interaction between the hPMCA4 isoform and theactin cytoskeleton may be of physiological relevance, we decided to further characterize itin the context of a living cell by monitoring in real-time the changes in the actinpolymerization and cytosolic Ca2+ concentration ([Ca2+]cyt). For this, hPMCA4 isoform wastransiently expressed in HEK293T cells. The dynamics of [Ca2+]cyt was performed usingthe fluorescent probe Fluo-4 and studying the alterations in [Ca2+]cyt generated by Ca2+release from the endoplasmic reticulum, and by extracellular Ca2+ entry through store-operated Ca2+ channels. The dynamics of actin polymerization was performed transientlyexpressing LifeAct-Ruby.Results show that the alteration of actin polymerization by CytD treatment significantlyincreased hPMCA4 activity (102%). On the other hand, in absent of CytD, actinpolymerization dynamics did not change after TG stimulus, while after Ca2+ stimulus, anactin reorganization was observed. This reorganization takes place at the same times thatthe hPMCA4 increases its activity, suggesting that hPMCA4 may be activated by actindepolymerization in the cells.
Fil: Vigil, Maximiliano Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Picco, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Rinaldi, Debora Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Rossi, Rolando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Rey, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Rossi, Juan Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Ferreira Gomes, Mariela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
XLIX Reunión Anual de la Sociedad Argentina de Biofísica
Argentina
Sociedad Argentina de Biofísica
Materia
PLASMA MEMBRANE CA2+-ATPASE
MODULATION
ACTIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173665
Acceder
id CONICETDig_b9e3198cd88f8a2f1c5a8c3e507cedab
oai_identifier_str oai:ri.conicet.gov.ar:11336/173665
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeletonVigil, Maximiliano AngelPicco, María ElisaRinaldi, Debora EugeniaRossi, Rolando CarlosRey, OsvaldoRossi, Juan Pablo FranciscoFerreira Gomes, Mariela SoledadPLASMA MEMBRANE CA2+-ATPASEMODULATIONACTINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Plasma Membrane Calcium ATPase (PMCA) is a calmodulin-modulated P-type ATPaseresponsible for the maintenance of low intracellular concentrations of Ca2+ in mosteukaryotic cells. Our group have previously shown that purified actin can exert a dualmodulation on the activity of Ca2+-ATPase 4b isoform (hPMCA4b): F-actin inhibits it whileshort actin oligomers may contribute to its activation. These studies had to be performedwith purified proteins given the nature of the biophysical and biochemical approachesused.On the other hand, in HEK293 human cells that overexpressed PMCA2w/b isoform, theactin depolymerization upon Cytochalasin D (CytD) treatment significantly increasedPMCA2-mediated Ca2+ extrusion and when F-actin was stabilized using jasplakinolide,PMCA2w/b activity was completely abolished.In order to assess whether the functional interaction between the hPMCA4 isoform and theactin cytoskeleton may be of physiological relevance, we decided to further characterize itin the context of a living cell by monitoring in real-time the changes in the actinpolymerization and cytosolic Ca2+ concentration ([Ca2+]cyt). For this, hPMCA4 isoform wastransiently expressed in HEK293T cells. The dynamics of [Ca2+]cyt was performed usingthe fluorescent probe Fluo-4 and studying the alterations in [Ca2+]cyt generated by Ca2+release from the endoplasmic reticulum, and by extracellular Ca2+ entry through store-operated Ca2+ channels. The dynamics of actin polymerization was performed transientlyexpressing LifeAct-Ruby.Results show that the alteration of actin polymerization by CytD treatment significantlyincreased hPMCA4 activity (102%). On the other hand, in absent of CytD, actinpolymerization dynamics did not change after TG stimulus, while after Ca2+ stimulus, anactin reorganization was observed. This reorganization takes place at the same times thatthe hPMCA4 increases its activity, suggesting that hPMCA4 may be activated by actindepolymerization in the cells.Fil: Vigil, Maximiliano Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Picco, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Rinaldi, Debora Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Rossi, Rolando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Rey, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Rossi, Juan Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Ferreira Gomes, Mariela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaXLIX Reunión Anual de la Sociedad Argentina de BiofísicaArgentinaSociedad Argentina de BiofísicaSociedad Argentina de Biofísica2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/173665Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton; XLIX Reunión Anual de la Sociedad Argentina de Biofísica; Argentina; 2021; 50-50978-987-27591-9-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:44Zoai:ri.conicet.gov.ar:11336/173665instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:44.914CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton
title Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton
spellingShingle Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton
Vigil, Maximiliano Angel
PLASMA MEMBRANE CA2+-ATPASE
MODULATION
ACTIN
title_short Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton
title_full Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton
title_fullStr Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton
title_full_unstemmed Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton
title_sort Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton
dc.creator.none.fl_str_mv Vigil, Maximiliano Angel
Picco, María Elisa
Rinaldi, Debora Eugenia
Rossi, Rolando Carlos
Rey, Osvaldo
Rossi, Juan Pablo Francisco
Ferreira Gomes, Mariela Soledad
author Vigil, Maximiliano Angel
author_facet Vigil, Maximiliano Angel
Picco, María Elisa
Rinaldi, Debora Eugenia
Rossi, Rolando Carlos
Rey, Osvaldo
Rossi, Juan Pablo Francisco
Ferreira Gomes, Mariela Soledad
author_role author
author2 Picco, María Elisa
Rinaldi, Debora Eugenia
Rossi, Rolando Carlos
Rey, Osvaldo
Rossi, Juan Pablo Francisco
Ferreira Gomes, Mariela Soledad
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PLASMA MEMBRANE CA2+-ATPASE
MODULATION
ACTIN
topic PLASMA MEMBRANE CA2+-ATPASE
MODULATION
ACTIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Plasma Membrane Calcium ATPase (PMCA) is a calmodulin-modulated P-type ATPaseresponsible for the maintenance of low intracellular concentrations of Ca2+ in mosteukaryotic cells. Our group have previously shown that purified actin can exert a dualmodulation on the activity of Ca2+-ATPase 4b isoform (hPMCA4b): F-actin inhibits it whileshort actin oligomers may contribute to its activation. These studies had to be performedwith purified proteins given the nature of the biophysical and biochemical approachesused.On the other hand, in HEK293 human cells that overexpressed PMCA2w/b isoform, theactin depolymerization upon Cytochalasin D (CytD) treatment significantly increasedPMCA2-mediated Ca2+ extrusion and when F-actin was stabilized using jasplakinolide,PMCA2w/b activity was completely abolished.In order to assess whether the functional interaction between the hPMCA4 isoform and theactin cytoskeleton may be of physiological relevance, we decided to further characterize itin the context of a living cell by monitoring in real-time the changes in the actinpolymerization and cytosolic Ca2+ concentration ([Ca2+]cyt). For this, hPMCA4 isoform wastransiently expressed in HEK293T cells. The dynamics of [Ca2+]cyt was performed usingthe fluorescent probe Fluo-4 and studying the alterations in [Ca2+]cyt generated by Ca2+release from the endoplasmic reticulum, and by extracellular Ca2+ entry through store-operated Ca2+ channels. The dynamics of actin polymerization was performed transientlyexpressing LifeAct-Ruby.Results show that the alteration of actin polymerization by CytD treatment significantlyincreased hPMCA4 activity (102%). On the other hand, in absent of CytD, actinpolymerization dynamics did not change after TG stimulus, while after Ca2+ stimulus, anactin reorganization was observed. This reorganization takes place at the same times thatthe hPMCA4 increases its activity, suggesting that hPMCA4 may be activated by actindepolymerization in the cells.
Fil: Vigil, Maximiliano Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Picco, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Rinaldi, Debora Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Rossi, Rolando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Rey, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Rossi, Juan Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Ferreira Gomes, Mariela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
XLIX Reunión Anual de la Sociedad Argentina de Biofísica
Argentina
Sociedad Argentina de Biofísica
description The Plasma Membrane Calcium ATPase (PMCA) is a calmodulin-modulated P-type ATPaseresponsible for the maintenance of low intracellular concentrations of Ca2+ in mosteukaryotic cells. Our group have previously shown that purified actin can exert a dualmodulation on the activity of Ca2+-ATPase 4b isoform (hPMCA4b): F-actin inhibits it whileshort actin oligomers may contribute to its activation. These studies had to be performedwith purified proteins given the nature of the biophysical and biochemical approachesused.On the other hand, in HEK293 human cells that overexpressed PMCA2w/b isoform, theactin depolymerization upon Cytochalasin D (CytD) treatment significantly increasedPMCA2-mediated Ca2+ extrusion and when F-actin was stabilized using jasplakinolide,PMCA2w/b activity was completely abolished.In order to assess whether the functional interaction between the hPMCA4 isoform and theactin cytoskeleton may be of physiological relevance, we decided to further characterize itin the context of a living cell by monitoring in real-time the changes in the actinpolymerization and cytosolic Ca2+ concentration ([Ca2+]cyt). For this, hPMCA4 isoform wastransiently expressed in HEK293T cells. The dynamics of [Ca2+]cyt was performed usingthe fluorescent probe Fluo-4 and studying the alterations in [Ca2+]cyt generated by Ca2+release from the endoplasmic reticulum, and by extracellular Ca2+ entry through store-operated Ca2+ channels. The dynamics of actin polymerization was performed transientlyexpressing LifeAct-Ruby.Results show that the alteration of actin polymerization by CytD treatment significantlyincreased hPMCA4 activity (102%). On the other hand, in absent of CytD, actinpolymerization dynamics did not change after TG stimulus, while after Ca2+ stimulus, anactin reorganization was observed. This reorganization takes place at the same times thatthe hPMCA4 increases its activity, suggesting that hPMCA4 may be activated by actindepolymerization in the cells.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173665
Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton; XLIX Reunión Anual de la Sociedad Argentina de Biofísica; Argentina; 2021; 50-50
978-987-27591-9-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173665
identifier_str_mv Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton; XLIX Reunión Anual de la Sociedad Argentina de Biofísica; Argentina; 2021; 50-50
978-987-27591-9-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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