Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimu...

Autores: Toso, Federico; Buldain, Daniel Cornelio; Retta, Daiana Sabrina; Di Leo Lira, Paola Maria del Rosario; Marchetti, Maria Laura; Mestorino, Olga Nora
Año de publicación: 2025
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The ban on antibiotic growth promoters (AGPs) in poultry farming has prompted the search for effective, natural alternatives. Essential oils (EOs), such as those from oregano (Origanum vulgare: OVEO) and rosemary (Salvia rosmarinus: ROEO), possess antimicrobial and antioxidant properties that may contribute to intestinal health and pathogen control. This study evaluated the antibacterial activity of OVEO and ROEO, individually and combined, against six Escherichia coli and six Salmonella typhimurium strains isolated from healthy poultry via cloacal swabs, as well as E. coli ATCC 25922 and S. typhimurium ATCC 14028 strains. The minimum inhibitory concentrations (MIC) were determined at five pH levels (7.4–5) simulating avian gastrointestinal tract conditions. EO composition was determined by GC-FID-MS. Checkerboard assays revealed partial or full synergistic effects at most pH, especially under acidic environments (pH ≤ 5.5), where the fractional inhibitory concentration (ΣFIC) values often indicated synergy. No antagonistic interactions were observed. These results suggest that OVEO and ROEO combinations are promising candidates to replace AGPs in poultry, particularly because of their enhanced efficacy under gastrointestinal pH. The strategic use of EO blends may reduce pathogen load, support performance, and limit antimicrobial resistance development, suggesting their potential as natural alternatives to AGPs under One Health principles.
Fil: Toso, Federico. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Buldain, Daniel Cornelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Retta, Daiana Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Di Leo Lira, Paola Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Marchetti, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Materia: OREGANO
ROSEMARY
ESSENTIAL OILS
E. COLI
S. TYPHIMURIUM
BROILER CHICKENS
CHECKERBOARD ASSAY
ANTIMICROBIAL SYNERGY
ANTIBIOTIC ALTERNATIVES
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/281663

Acceder

id	CONICETDig_b9bf2d4290e47a06b2a896fba3185ff0
oai_identifier_str	oai:ri.conicet.gov.ar:11336/281663
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from PoultryToso, FedericoBuldain, Daniel CornelioRetta, Daiana SabrinaDi Leo Lira, Paola Maria del RosarioMarchetti, Maria LauraMestorino, Olga NoraOREGANOROSEMARYESSENTIAL OILSE. COLIS. TYPHIMURIUMBROILER CHICKENSCHECKERBOARD ASSAYANTIMICROBIAL SYNERGYANTIBIOTIC ALTERNATIVEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The ban on antibiotic growth promoters (AGPs) in poultry farming has prompted the search for effective, natural alternatives. Essential oils (EOs), such as those from oregano (Origanum vulgare: OVEO) and rosemary (Salvia rosmarinus: ROEO), possess antimicrobial and antioxidant properties that may contribute to intestinal health and pathogen control. This study evaluated the antibacterial activity of OVEO and ROEO, individually and combined, against six Escherichia coli and six Salmonella typhimurium strains isolated from healthy poultry via cloacal swabs, as well as E. coli ATCC 25922 and S. typhimurium ATCC 14028 strains. The minimum inhibitory concentrations (MIC) were determined at five pH levels (7.4–5) simulating avian gastrointestinal tract conditions. EO composition was determined by GC-FID-MS. Checkerboard assays revealed partial or full synergistic effects at most pH, especially under acidic environments (pH ≤ 5.5), where the fractional inhibitory concentration (ΣFIC) values often indicated synergy. No antagonistic interactions were observed. These results suggest that OVEO and ROEO combinations are promising candidates to replace AGPs in poultry, particularly because of their enhanced efficacy under gastrointestinal pH. The strategic use of EO blends may reduce pathogen load, support performance, and limit antimicrobial resistance development, suggesting their potential as natural alternatives to AGPs under One Health principles.Fil: Toso, Federico. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaFil: Buldain, Daniel Cornelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaFil: Retta, Daiana Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Di Leo Lira, Paola Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Marchetti, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaFil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaMultidisciplinary Digital Publishing Institute2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281663Toso, Federico; Buldain, Daniel Cornelio; Retta, Daiana Sabrina; Di Leo Lira, Paola Maria del Rosario; Marchetti, Maria Laura; et al.; Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry; Multidisciplinary Digital Publishing Institute; Processes; 13; 9; 9-2025; 1-182227-9717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2227-9717/13/9/2856info:eu-repo/semantics/altIdentifier/doi/10.3390/pr13092856info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-31T15:22:56Zoai:ri.conicet.gov.ar:11336/281663instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-31 15:22:56.544CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry
title	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry
spellingShingle	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry Toso, Federico OREGANO ROSEMARY ESSENTIAL OILS E. COLI S. TYPHIMURIUM BROILER CHICKENS CHECKERBOARD ASSAY ANTIMICROBIAL SYNERGY ANTIBIOTIC ALTERNATIVES
title_short	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry
title_full	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry
title_fullStr	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry
title_full_unstemmed	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry
title_sort	Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry
dc.creator.none.fl_str_mv	Toso, Federico Buldain, Daniel Cornelio Retta, Daiana Sabrina Di Leo Lira, Paola Maria del Rosario Marchetti, Maria Laura Mestorino, Olga Nora
author	Toso, Federico
author_facet	Toso, Federico Buldain, Daniel Cornelio Retta, Daiana Sabrina Di Leo Lira, Paola Maria del Rosario Marchetti, Maria Laura Mestorino, Olga Nora
author_role	author
author2	Buldain, Daniel Cornelio Retta, Daiana Sabrina Di Leo Lira, Paola Maria del Rosario Marchetti, Maria Laura Mestorino, Olga Nora
author2_role	author author author author author
dc.subject.none.fl_str_mv	OREGANO ROSEMARY ESSENTIAL OILS E. COLI S. TYPHIMURIUM BROILER CHICKENS CHECKERBOARD ASSAY ANTIMICROBIAL SYNERGY ANTIBIOTIC ALTERNATIVES
topic	OREGANO ROSEMARY ESSENTIAL OILS E. COLI S. TYPHIMURIUM BROILER CHICKENS CHECKERBOARD ASSAY ANTIMICROBIAL SYNERGY ANTIBIOTIC ALTERNATIVES
purl_subject.fl_str_mv	https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv	The ban on antibiotic growth promoters (AGPs) in poultry farming has prompted the search for effective, natural alternatives. Essential oils (EOs), such as those from oregano (Origanum vulgare: OVEO) and rosemary (Salvia rosmarinus: ROEO), possess antimicrobial and antioxidant properties that may contribute to intestinal health and pathogen control. This study evaluated the antibacterial activity of OVEO and ROEO, individually and combined, against six Escherichia coli and six Salmonella typhimurium strains isolated from healthy poultry via cloacal swabs, as well as E. coli ATCC 25922 and S. typhimurium ATCC 14028 strains. The minimum inhibitory concentrations (MIC) were determined at five pH levels (7.4–5) simulating avian gastrointestinal tract conditions. EO composition was determined by GC-FID-MS. Checkerboard assays revealed partial or full synergistic effects at most pH, especially under acidic environments (pH ≤ 5.5), where the fractional inhibitory concentration (ΣFIC) values often indicated synergy. No antagonistic interactions were observed. These results suggest that OVEO and ROEO combinations are promising candidates to replace AGPs in poultry, particularly because of their enhanced efficacy under gastrointestinal pH. The strategic use of EO blends may reduce pathogen load, support performance, and limit antimicrobial resistance development, suggesting their potential as natural alternatives to AGPs under One Health principles. Fil: Toso, Federico. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina Fil: Buldain, Daniel Cornelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina Fil: Retta, Daiana Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Di Leo Lira, Paola Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Marchetti, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina Fil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
description	The ban on antibiotic growth promoters (AGPs) in poultry farming has prompted the search for effective, natural alternatives. Essential oils (EOs), such as those from oregano (Origanum vulgare: OVEO) and rosemary (Salvia rosmarinus: ROEO), possess antimicrobial and antioxidant properties that may contribute to intestinal health and pathogen control. This study evaluated the antibacterial activity of OVEO and ROEO, individually and combined, against six Escherichia coli and six Salmonella typhimurium strains isolated from healthy poultry via cloacal swabs, as well as E. coli ATCC 25922 and S. typhimurium ATCC 14028 strains. The minimum inhibitory concentrations (MIC) were determined at five pH levels (7.4–5) simulating avian gastrointestinal tract conditions. EO composition was determined by GC-FID-MS. Checkerboard assays revealed partial or full synergistic effects at most pH, especially under acidic environments (pH ≤ 5.5), where the fractional inhibitory concentration (ΣFIC) values often indicated synergy. No antagonistic interactions were observed. These results suggest that OVEO and ROEO combinations are promising candidates to replace AGPs in poultry, particularly because of their enhanced efficacy under gastrointestinal pH. The strategic use of EO blends may reduce pathogen load, support performance, and limit antimicrobial resistance development, suggesting their potential as natural alternatives to AGPs under One Health principles.
publishDate	2025
dc.date.none.fl_str_mv	2025-09
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/281663 Toso, Federico; Buldain, Daniel Cornelio; Retta, Daiana Sabrina; Di Leo Lira, Paola Maria del Rosario; Marchetti, Maria Laura; et al.; Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry; Multidisciplinary Digital Publishing Institute; Processes; 13; 9; 9-2025; 1-18 2227-9717 CONICET Digital CONICET
url	http://hdl.handle.net/11336/281663
identifier_str_mv	Toso, Federico; Buldain, Daniel Cornelio; Retta, Daiana Sabrina; Di Leo Lira, Paola Maria del Rosario; Marchetti, Maria Laura; et al.; Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimurium Isolated from Poultry; Multidisciplinary Digital Publishing Institute; Processes; 13; 9; 9-2025; 1-18 2227-9717 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2227-9717/13/9/2856 info:eu-repo/semantics/altIdentifier/doi/10.3390/pr13092856
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1861214683310063616
score	12.822162

Antimicrobial Activity of Origanum vulgare L. And Salvia rosmarinus Spenn (syn Rosmarinus officinalis L.) Essential Oil Combinations Against Escherichia coli and Salmonella typhimu...

Publicaciones similares