A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge

Autores
Borrego, Belén; Argilaguet, Jordi M.; Pérez Martín, Eva; Dominguez, Javier; Pérez Filgueira, Daniel Mariano; Escribano, José M.; Sobrino, Francisco; Rodriguez, Fernando
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Development of efficient and safer vaccines against foot-and-mouth disease virus (FMDV) is a must. Previous results obtained in our laboratory have demonstrated that DNA vaccines encoding B and T cell epitopes from type C FMDV, efficiently controlled virus replication in mice, while they did not protect against FMDV challenge in pigs, one of the FMDV natural hosts. The main finding of this work is the ability to improve the protection afforded in swine using a new DNA-vaccine prototype (pCMV-APCH1BTT), encoding FMDV B and T-cell epitopes fused to the single-chain variable fragment of the 1F12 mouse monoclonal antibody that recognizes Class-II Swine Leukocyte antigens. Half of the DNA-immunized pigs were fully protected upon viral challenge, while the remaining animals were partially protected, showing a delayed, shorter and milder disease than control pigs. Full protection in a given vaccinated-pig correlated with the induction of specific IFNγ-secreting T-cells, detectable prior to FMDV-challenge, together with a rapid development of neutralizing antibodies after viral challenge, pointing towards the relevance that both arms of the immune response can play in protection. Our results open new avenues for developing future FMDV subunit vaccines.
Fil: Borrego, Belén. No especifíca;
Fil: Argilaguet, Jordi M.. Universitat Autònoma de Barcelona; España
Fil: Pérez Martín, Eva. Universitat Autònoma de Barcelona; España
Fil: Dominguez, Javier. No especifíca;
Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escribano, José M.. No especifíca;
Fil: Sobrino, Francisco. No especifíca;
Fil: Rodriguez, Fernando. Universitat Autònoma de Barcelona; España
Materia
DNA VACCINE
FMDV
NEUTRALIZING ANTIBODY
SCFV
T-CELL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193887
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/193887
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challengeBorrego, BelénArgilaguet, Jordi M.Pérez Martín, EvaDominguez, JavierPérez Filgueira, Daniel MarianoEscribano, José M.Sobrino, FranciscoRodriguez, FernandoDNA VACCINEFMDVNEUTRALIZING ANTIBODYSCFVT-CELLhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Development of efficient and safer vaccines against foot-and-mouth disease virus (FMDV) is a must. Previous results obtained in our laboratory have demonstrated that DNA vaccines encoding B and T cell epitopes from type C FMDV, efficiently controlled virus replication in mice, while they did not protect against FMDV challenge in pigs, one of the FMDV natural hosts. The main finding of this work is the ability to improve the protection afforded in swine using a new DNA-vaccine prototype (pCMV-APCH1BTT), encoding FMDV B and T-cell epitopes fused to the single-chain variable fragment of the 1F12 mouse monoclonal antibody that recognizes Class-II Swine Leukocyte antigens. Half of the DNA-immunized pigs were fully protected upon viral challenge, while the remaining animals were partially protected, showing a delayed, shorter and milder disease than control pigs. Full protection in a given vaccinated-pig correlated with the induction of specific IFNγ-secreting T-cells, detectable prior to FMDV-challenge, together with a rapid development of neutralizing antibodies after viral challenge, pointing towards the relevance that both arms of the immune response can play in protection. Our results open new avenues for developing future FMDV subunit vaccines.Fil: Borrego, Belén. No especifíca;Fil: Argilaguet, Jordi M.. Universitat Autònoma de Barcelona; EspañaFil: Pérez Martín, Eva. Universitat Autònoma de Barcelona; EspañaFil: Dominguez, Javier. No especifíca;Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escribano, José M.. No especifíca;Fil: Sobrino, Francisco. No especifíca;Fil: Rodriguez, Fernando. Universitat Autònoma de Barcelona; EspañaElsevier Science2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193887Borrego, Belén; Argilaguet, Jordi M.; Pérez Martín, Eva; Dominguez, Javier; Pérez Filgueira, Daniel Mariano; et al.; A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge; Elsevier Science; Antiviral Research; 92; 2; 11-2011; 359-3630166-3542CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0166354211003883info:eu-repo/semantics/altIdentifier/doi/10.1016/j.antiviral.2011.07.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:02:05Zoai:ri.conicet.gov.ar:11336/193887instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:02:05.43CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge
title A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge
spellingShingle A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge
Borrego, Belén
DNA VACCINE
FMDV
NEUTRALIZING ANTIBODY
SCFV
T-CELL
title_short A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge
title_full A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge
title_fullStr A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge
title_full_unstemmed A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge
title_sort A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge
dc.creator.none.fl_str_mv Borrego, Belén
Argilaguet, Jordi M.
Pérez Martín, Eva
Dominguez, Javier
Pérez Filgueira, Daniel Mariano
Escribano, José M.
Sobrino, Francisco
Rodriguez, Fernando
author Borrego, Belén
author_facet Borrego, Belén
Argilaguet, Jordi M.
Pérez Martín, Eva
Dominguez, Javier
Pérez Filgueira, Daniel Mariano
Escribano, José M.
Sobrino, Francisco
Rodriguez, Fernando
author_role author
author2 Argilaguet, Jordi M.
Pérez Martín, Eva
Dominguez, Javier
Pérez Filgueira, Daniel Mariano
Escribano, José M.
Sobrino, Francisco
Rodriguez, Fernando
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DNA VACCINE
FMDV
NEUTRALIZING ANTIBODY
SCFV
T-CELL
topic DNA VACCINE
FMDV
NEUTRALIZING ANTIBODY
SCFV
T-CELL
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Development of efficient and safer vaccines against foot-and-mouth disease virus (FMDV) is a must. Previous results obtained in our laboratory have demonstrated that DNA vaccines encoding B and T cell epitopes from type C FMDV, efficiently controlled virus replication in mice, while they did not protect against FMDV challenge in pigs, one of the FMDV natural hosts. The main finding of this work is the ability to improve the protection afforded in swine using a new DNA-vaccine prototype (pCMV-APCH1BTT), encoding FMDV B and T-cell epitopes fused to the single-chain variable fragment of the 1F12 mouse monoclonal antibody that recognizes Class-II Swine Leukocyte antigens. Half of the DNA-immunized pigs were fully protected upon viral challenge, while the remaining animals were partially protected, showing a delayed, shorter and milder disease than control pigs. Full protection in a given vaccinated-pig correlated with the induction of specific IFNγ-secreting T-cells, detectable prior to FMDV-challenge, together with a rapid development of neutralizing antibodies after viral challenge, pointing towards the relevance that both arms of the immune response can play in protection. Our results open new avenues for developing future FMDV subunit vaccines.
Fil: Borrego, Belén. No especifíca;
Fil: Argilaguet, Jordi M.. Universitat Autònoma de Barcelona; España
Fil: Pérez Martín, Eva. Universitat Autònoma de Barcelona; España
Fil: Dominguez, Javier. No especifíca;
Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escribano, José M.. No especifíca;
Fil: Sobrino, Francisco. No especifíca;
Fil: Rodriguez, Fernando. Universitat Autònoma de Barcelona; España
description Development of efficient and safer vaccines against foot-and-mouth disease virus (FMDV) is a must. Previous results obtained in our laboratory have demonstrated that DNA vaccines encoding B and T cell epitopes from type C FMDV, efficiently controlled virus replication in mice, while they did not protect against FMDV challenge in pigs, one of the FMDV natural hosts. The main finding of this work is the ability to improve the protection afforded in swine using a new DNA-vaccine prototype (pCMV-APCH1BTT), encoding FMDV B and T-cell epitopes fused to the single-chain variable fragment of the 1F12 mouse monoclonal antibody that recognizes Class-II Swine Leukocyte antigens. Half of the DNA-immunized pigs were fully protected upon viral challenge, while the remaining animals were partially protected, showing a delayed, shorter and milder disease than control pigs. Full protection in a given vaccinated-pig correlated with the induction of specific IFNγ-secreting T-cells, detectable prior to FMDV-challenge, together with a rapid development of neutralizing antibodies after viral challenge, pointing towards the relevance that both arms of the immune response can play in protection. Our results open new avenues for developing future FMDV subunit vaccines.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193887
Borrego, Belén; Argilaguet, Jordi M.; Pérez Martín, Eva; Dominguez, Javier; Pérez Filgueira, Daniel Mariano; et al.; A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge; Elsevier Science; Antiviral Research; 92; 2; 11-2011; 359-363
0166-3542
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193887
identifier_str_mv Borrego, Belén; Argilaguet, Jordi M.; Pérez Martín, Eva; Dominguez, Javier; Pérez Filgueira, Daniel Mariano; et al.; A DNA vaccine encoding foot-and-mouth disease virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs against viral challenge; Elsevier Science; Antiviral Research; 92; 2; 11-2011; 359-363
0166-3542
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0166354211003883
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.antiviral.2011.07.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083161937674240
score 13.22299

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