Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
- Autores
- Argilaguet, J. M.; Pérez Martín, E.; Gallardo, C.; Salguero, F. J.; Borrego, B.; Lacasta, A.; Accensi, F.; Díaz, I.; Nofrarías, M.; Pujols, J.; Blanco, E.; Pérez Filgueira, Daniel Mariano; Escribano, José M.; Rodríguez, F.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- One of the main criticisms to DNA vaccines is the poor immunogenicity that they confer on occasions, at least in large animals. Confirming this theory, immunization with plasmid DNA encoding two African swine fever virus genes in frame (pCMV-PQ), failed in inducing detectable immune responses in pigs, while it was successful in mice. Aiming to improve the immune responses induced in swine, a new plasmid was constructed, encoding the viral genes fused in frame with a single chain variable fragment of an antibody specific for a swine leukocyte antigen II (pCMV-APCH1PQ). Our results clearly demonstrate that targeting antigens to antigen professional cells exponentially enhanced the immune response induced in pigs, albeit that the DNA vaccine was not able to confer protection against lethal viral challenge. Indeed, a viremia exacerbation was observed in each of the pigs that received the pCMV-APCH1PQ plasmid, this correlating with the presence of non-neutralizing antibodies and antigen-specific SLA II-restricted T-cells. The implications of our discoveries for the development of future vaccines against African swine fever virus and other swine pathogens are discussed.
Fil: Argilaguet, J. M.. No especifíca;
Fil: Pérez Martín, E.. No especifíca;
Fil: Gallardo, C.. No especifíca;
Fil: Salguero, F. J.. No especifíca;
Fil: Borrego, B.. No especifíca;
Fil: Lacasta, A.. No especifíca;
Fil: Accensi, F.. No especifíca;
Fil: Díaz, I.. No especifíca;
Fil: Nofrarías, M.. No especifíca;
Fil: Pujols, J.. No especifíca;
Fil: Blanco, E.. No especifíca;
Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escribano, José M.. No especifíca;
Fil: Rodríguez, F.. No especifíca; - Materia
-
ASFV
DNA VACCINE
SCFV - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/192540
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/192540 |
network_acronym_str |
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network_name_str |
CONICET Digital (CONICET) |
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Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cellsArgilaguet, J. M.Pérez Martín, E.Gallardo, C.Salguero, F. J.Borrego, B.Lacasta, A.Accensi, F.Díaz, I.Nofrarías, M.Pujols, J.Blanco, E.Pérez Filgueira, Daniel MarianoEscribano, José M.Rodríguez, F.ASFVDNA VACCINESCFVhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4One of the main criticisms to DNA vaccines is the poor immunogenicity that they confer on occasions, at least in large animals. Confirming this theory, immunization with plasmid DNA encoding two African swine fever virus genes in frame (pCMV-PQ), failed in inducing detectable immune responses in pigs, while it was successful in mice. Aiming to improve the immune responses induced in swine, a new plasmid was constructed, encoding the viral genes fused in frame with a single chain variable fragment of an antibody specific for a swine leukocyte antigen II (pCMV-APCH1PQ). Our results clearly demonstrate that targeting antigens to antigen professional cells exponentially enhanced the immune response induced in pigs, albeit that the DNA vaccine was not able to confer protection against lethal viral challenge. Indeed, a viremia exacerbation was observed in each of the pigs that received the pCMV-APCH1PQ plasmid, this correlating with the presence of non-neutralizing antibodies and antigen-specific SLA II-restricted T-cells. The implications of our discoveries for the development of future vaccines against African swine fever virus and other swine pathogens are discussed.Fil: Argilaguet, J. M.. No especifíca;Fil: Pérez Martín, E.. No especifíca;Fil: Gallardo, C.. No especifíca;Fil: Salguero, F. J.. No especifíca;Fil: Borrego, B.. No especifíca;Fil: Lacasta, A.. No especifíca;Fil: Accensi, F.. No especifíca;Fil: Díaz, I.. No especifíca;Fil: Nofrarías, M.. No especifíca;Fil: Pujols, J.. No especifíca;Fil: Blanco, E.. No especifíca;Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escribano, José M.. No especifíca;Fil: Rodríguez, F.. No especifíca;Elsevier2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192540Argilaguet, J. M.; Pérez Martín, E.; Gallardo, C.; Salguero, F. J.; Borrego, B.; et al.; Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells; Elsevier; Vaccine; 29; 33; 6-2011; 5379-53850264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X11008310info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2011.05.084info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:12Zoai:ri.conicet.gov.ar:11336/192540instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:12.851CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells |
title |
Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells |
spellingShingle |
Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells Argilaguet, J. M. ASFV DNA VACCINE SCFV |
title_short |
Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells |
title_full |
Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells |
title_fullStr |
Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells |
title_full_unstemmed |
Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells |
title_sort |
Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells |
dc.creator.none.fl_str_mv |
Argilaguet, J. M. Pérez Martín, E. Gallardo, C. Salguero, F. J. Borrego, B. Lacasta, A. Accensi, F. Díaz, I. Nofrarías, M. Pujols, J. Blanco, E. Pérez Filgueira, Daniel Mariano Escribano, José M. Rodríguez, F. |
author |
Argilaguet, J. M. |
author_facet |
Argilaguet, J. M. Pérez Martín, E. Gallardo, C. Salguero, F. J. Borrego, B. Lacasta, A. Accensi, F. Díaz, I. Nofrarías, M. Pujols, J. Blanco, E. Pérez Filgueira, Daniel Mariano Escribano, José M. Rodríguez, F. |
author_role |
author |
author2 |
Pérez Martín, E. Gallardo, C. Salguero, F. J. Borrego, B. Lacasta, A. Accensi, F. Díaz, I. Nofrarías, M. Pujols, J. Blanco, E. Pérez Filgueira, Daniel Mariano Escribano, José M. Rodríguez, F. |
author2_role |
author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
ASFV DNA VACCINE SCFV |
topic |
ASFV DNA VACCINE SCFV |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
One of the main criticisms to DNA vaccines is the poor immunogenicity that they confer on occasions, at least in large animals. Confirming this theory, immunization with plasmid DNA encoding two African swine fever virus genes in frame (pCMV-PQ), failed in inducing detectable immune responses in pigs, while it was successful in mice. Aiming to improve the immune responses induced in swine, a new plasmid was constructed, encoding the viral genes fused in frame with a single chain variable fragment of an antibody specific for a swine leukocyte antigen II (pCMV-APCH1PQ). Our results clearly demonstrate that targeting antigens to antigen professional cells exponentially enhanced the immune response induced in pigs, albeit that the DNA vaccine was not able to confer protection against lethal viral challenge. Indeed, a viremia exacerbation was observed in each of the pigs that received the pCMV-APCH1PQ plasmid, this correlating with the presence of non-neutralizing antibodies and antigen-specific SLA II-restricted T-cells. The implications of our discoveries for the development of future vaccines against African swine fever virus and other swine pathogens are discussed. Fil: Argilaguet, J. M.. No especifíca; Fil: Pérez Martín, E.. No especifíca; Fil: Gallardo, C.. No especifíca; Fil: Salguero, F. J.. No especifíca; Fil: Borrego, B.. No especifíca; Fil: Lacasta, A.. No especifíca; Fil: Accensi, F.. No especifíca; Fil: Díaz, I.. No especifíca; Fil: Nofrarías, M.. No especifíca; Fil: Pujols, J.. No especifíca; Fil: Blanco, E.. No especifíca; Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Escribano, José M.. No especifíca; Fil: Rodríguez, F.. No especifíca; |
description |
One of the main criticisms to DNA vaccines is the poor immunogenicity that they confer on occasions, at least in large animals. Confirming this theory, immunization with plasmid DNA encoding two African swine fever virus genes in frame (pCMV-PQ), failed in inducing detectable immune responses in pigs, while it was successful in mice. Aiming to improve the immune responses induced in swine, a new plasmid was constructed, encoding the viral genes fused in frame with a single chain variable fragment of an antibody specific for a swine leukocyte antigen II (pCMV-APCH1PQ). Our results clearly demonstrate that targeting antigens to antigen professional cells exponentially enhanced the immune response induced in pigs, albeit that the DNA vaccine was not able to confer protection against lethal viral challenge. Indeed, a viremia exacerbation was observed in each of the pigs that received the pCMV-APCH1PQ plasmid, this correlating with the presence of non-neutralizing antibodies and antigen-specific SLA II-restricted T-cells. The implications of our discoveries for the development of future vaccines against African swine fever virus and other swine pathogens are discussed. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/192540 Argilaguet, J. M.; Pérez Martín, E.; Gallardo, C.; Salguero, F. J.; Borrego, B.; et al.; Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells; Elsevier; Vaccine; 29; 33; 6-2011; 5379-5385 0264-410X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/192540 |
identifier_str_mv |
Argilaguet, J. M.; Pérez Martín, E.; Gallardo, C.; Salguero, F. J.; Borrego, B.; et al.; Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells; Elsevier; Vaccine; 29; 33; 6-2011; 5379-5385 0264-410X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X11008310 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2011.05.084 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846781737109028864 |
score |
13.229304 |