Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells

Autores
Argilaguet, J. M.; Pérez Martín, E.; Gallardo, C.; Salguero, F. J.; Borrego, B.; Lacasta, A.; Accensi, F.; Díaz, I.; Nofrarías, M.; Pujols, J.; Blanco, E.; Pérez Filgueira, Daniel Mariano; Escribano, José M.; Rodríguez, F.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the main criticisms to DNA vaccines is the poor immunogenicity that they confer on occasions, at least in large animals. Confirming this theory, immunization with plasmid DNA encoding two African swine fever virus genes in frame (pCMV-PQ), failed in inducing detectable immune responses in pigs, while it was successful in mice. Aiming to improve the immune responses induced in swine, a new plasmid was constructed, encoding the viral genes fused in frame with a single chain variable fragment of an antibody specific for a swine leukocyte antigen II (pCMV-APCH1PQ). Our results clearly demonstrate that targeting antigens to antigen professional cells exponentially enhanced the immune response induced in pigs, albeit that the DNA vaccine was not able to confer protection against lethal viral challenge. Indeed, a viremia exacerbation was observed in each of the pigs that received the pCMV-APCH1PQ plasmid, this correlating with the presence of non-neutralizing antibodies and antigen-specific SLA II-restricted T-cells. The implications of our discoveries for the development of future vaccines against African swine fever virus and other swine pathogens are discussed.
Fil: Argilaguet, J. M.. No especifíca;
Fil: Pérez Martín, E.. No especifíca;
Fil: Gallardo, C.. No especifíca;
Fil: Salguero, F. J.. No especifíca;
Fil: Borrego, B.. No especifíca;
Fil: Lacasta, A.. No especifíca;
Fil: Accensi, F.. No especifíca;
Fil: Díaz, I.. No especifíca;
Fil: Nofrarías, M.. No especifíca;
Fil: Pujols, J.. No especifíca;
Fil: Blanco, E.. No especifíca;
Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escribano, José M.. No especifíca;
Fil: Rodríguez, F.. No especifíca;
Materia
ASFV
DNA VACCINE
SCFV
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192540

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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cellsArgilaguet, J. M.Pérez Martín, E.Gallardo, C.Salguero, F. J.Borrego, B.Lacasta, A.Accensi, F.Díaz, I.Nofrarías, M.Pujols, J.Blanco, E.Pérez Filgueira, Daniel MarianoEscribano, José M.Rodríguez, F.ASFVDNA VACCINESCFVhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4One of the main criticisms to DNA vaccines is the poor immunogenicity that they confer on occasions, at least in large animals. Confirming this theory, immunization with plasmid DNA encoding two African swine fever virus genes in frame (pCMV-PQ), failed in inducing detectable immune responses in pigs, while it was successful in mice. Aiming to improve the immune responses induced in swine, a new plasmid was constructed, encoding the viral genes fused in frame with a single chain variable fragment of an antibody specific for a swine leukocyte antigen II (pCMV-APCH1PQ). Our results clearly demonstrate that targeting antigens to antigen professional cells exponentially enhanced the immune response induced in pigs, albeit that the DNA vaccine was not able to confer protection against lethal viral challenge. Indeed, a viremia exacerbation was observed in each of the pigs that received the pCMV-APCH1PQ plasmid, this correlating with the presence of non-neutralizing antibodies and antigen-specific SLA II-restricted T-cells. The implications of our discoveries for the development of future vaccines against African swine fever virus and other swine pathogens are discussed.Fil: Argilaguet, J. M.. No especifíca;Fil: Pérez Martín, E.. No especifíca;Fil: Gallardo, C.. No especifíca;Fil: Salguero, F. J.. No especifíca;Fil: Borrego, B.. No especifíca;Fil: Lacasta, A.. No especifíca;Fil: Accensi, F.. No especifíca;Fil: Díaz, I.. No especifíca;Fil: Nofrarías, M.. No especifíca;Fil: Pujols, J.. No especifíca;Fil: Blanco, E.. No especifíca;Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escribano, José M.. No especifíca;Fil: Rodríguez, F.. No especifíca;Elsevier2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192540Argilaguet, J. M.; Pérez Martín, E.; Gallardo, C.; Salguero, F. J.; Borrego, B.; et al.; Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells; Elsevier; Vaccine; 29; 33; 6-2011; 5379-53850264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X11008310info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2011.05.084info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:12Zoai:ri.conicet.gov.ar:11336/192540instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:12.851CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
title Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
spellingShingle Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
Argilaguet, J. M.
ASFV
DNA VACCINE
SCFV
title_short Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
title_full Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
title_fullStr Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
title_full_unstemmed Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
title_sort Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells
dc.creator.none.fl_str_mv Argilaguet, J. M.
Pérez Martín, E.
Gallardo, C.
Salguero, F. J.
Borrego, B.
Lacasta, A.
Accensi, F.
Díaz, I.
Nofrarías, M.
Pujols, J.
Blanco, E.
Pérez Filgueira, Daniel Mariano
Escribano, José M.
Rodríguez, F.
author Argilaguet, J. M.
author_facet Argilaguet, J. M.
Pérez Martín, E.
Gallardo, C.
Salguero, F. J.
Borrego, B.
Lacasta, A.
Accensi, F.
Díaz, I.
Nofrarías, M.
Pujols, J.
Blanco, E.
Pérez Filgueira, Daniel Mariano
Escribano, José M.
Rodríguez, F.
author_role author
author2 Pérez Martín, E.
Gallardo, C.
Salguero, F. J.
Borrego, B.
Lacasta, A.
Accensi, F.
Díaz, I.
Nofrarías, M.
Pujols, J.
Blanco, E.
Pérez Filgueira, Daniel Mariano
Escribano, José M.
Rodríguez, F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ASFV
DNA VACCINE
SCFV
topic ASFV
DNA VACCINE
SCFV
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv One of the main criticisms to DNA vaccines is the poor immunogenicity that they confer on occasions, at least in large animals. Confirming this theory, immunization with plasmid DNA encoding two African swine fever virus genes in frame (pCMV-PQ), failed in inducing detectable immune responses in pigs, while it was successful in mice. Aiming to improve the immune responses induced in swine, a new plasmid was constructed, encoding the viral genes fused in frame with a single chain variable fragment of an antibody specific for a swine leukocyte antigen II (pCMV-APCH1PQ). Our results clearly demonstrate that targeting antigens to antigen professional cells exponentially enhanced the immune response induced in pigs, albeit that the DNA vaccine was not able to confer protection against lethal viral challenge. Indeed, a viremia exacerbation was observed in each of the pigs that received the pCMV-APCH1PQ plasmid, this correlating with the presence of non-neutralizing antibodies and antigen-specific SLA II-restricted T-cells. The implications of our discoveries for the development of future vaccines against African swine fever virus and other swine pathogens are discussed.
Fil: Argilaguet, J. M.. No especifíca;
Fil: Pérez Martín, E.. No especifíca;
Fil: Gallardo, C.. No especifíca;
Fil: Salguero, F. J.. No especifíca;
Fil: Borrego, B.. No especifíca;
Fil: Lacasta, A.. No especifíca;
Fil: Accensi, F.. No especifíca;
Fil: Díaz, I.. No especifíca;
Fil: Nofrarías, M.. No especifíca;
Fil: Pujols, J.. No especifíca;
Fil: Blanco, E.. No especifíca;
Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escribano, José M.. No especifíca;
Fil: Rodríguez, F.. No especifíca;
description One of the main criticisms to DNA vaccines is the poor immunogenicity that they confer on occasions, at least in large animals. Confirming this theory, immunization with plasmid DNA encoding two African swine fever virus genes in frame (pCMV-PQ), failed in inducing detectable immune responses in pigs, while it was successful in mice. Aiming to improve the immune responses induced in swine, a new plasmid was constructed, encoding the viral genes fused in frame with a single chain variable fragment of an antibody specific for a swine leukocyte antigen II (pCMV-APCH1PQ). Our results clearly demonstrate that targeting antigens to antigen professional cells exponentially enhanced the immune response induced in pigs, albeit that the DNA vaccine was not able to confer protection against lethal viral challenge. Indeed, a viremia exacerbation was observed in each of the pigs that received the pCMV-APCH1PQ plasmid, this correlating with the presence of non-neutralizing antibodies and antigen-specific SLA II-restricted T-cells. The implications of our discoveries for the development of future vaccines against African swine fever virus and other swine pathogens are discussed.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192540
Argilaguet, J. M.; Pérez Martín, E.; Gallardo, C.; Salguero, F. J.; Borrego, B.; et al.; Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells; Elsevier; Vaccine; 29; 33; 6-2011; 5379-5385
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192540
identifier_str_mv Argilaguet, J. M.; Pérez Martín, E.; Gallardo, C.; Salguero, F. J.; Borrego, B.; et al.; Enhancing dna immunization by targeting asfv antigens to sla-ii bearing cells; Elsevier; Vaccine; 29; 33; 6-2011; 5379-5385
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X11008310
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2011.05.084
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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