Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase

Autores
Castro, Claudia Magdalena; Gil Lorenzo, Andrea Fernanda; Gonzalez, Adriana; Cruzado, Montserrat Cecilia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Garlic has been used for prevention and treatment of hypertension; however the molecular mechanisms of garlic’s effects remain to be elucidated. In the present study, the mechanisms of the in vitro effect of organosulphur compounds derived from garlic on growth and migration of cultured aortic smooth muscle cells (AoSMC) isolated from spontaneously hypertensive rats (SHR) were investigated. We demonstrated that Allyl methyl sulphide (AMS) and Diallyl sulphide (DAS), inhibited AoSMC angiotensin II-stimulated cell cycle progression and migration. Neither cell viability nor annexin-V binding analysis revealed cytotoxic effects of both organosulphur compounds at the used concentrations. Instead, their inhibitory effects were associated to the prevention of the cell cycle inhibitor p27Kip1 (p27) downregulation and the reduction of ERK 1/2 phosphorilation. When we assessed the antioxidant activity of AMS and DAS we found that both organosulphur compounds inhibited angiotensin II- reactive oxygen species (ROS) generation. Our findings show that AMS and DAS, compounds derivate from garlic, could be effective antioxidants targeted at the arterial remodeling seen in hypertension.
Fil: Castro, Claudia Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Gil Lorenzo, Andrea Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Gonzalez, Adriana. Universidad Nacional de Cuyo; Argentina
Fil: Cruzado, Montserrat Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
ANGIOTENSIN II
CELL CYCLE
GARLIC
HYPERTENSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280059
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/280059
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinaseCastro, Claudia MagdalenaGil Lorenzo, Andrea FernandaGonzalez, AdrianaCruzado, Montserrat CeciliaANGIOTENSIN IICELL CYCLEGARLICHYPERTENSIONhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Garlic has been used for prevention and treatment of hypertension; however the molecular mechanisms of garlic’s effects remain to be elucidated. In the present study, the mechanisms of the in vitro effect of organosulphur compounds derived from garlic on growth and migration of cultured aortic smooth muscle cells (AoSMC) isolated from spontaneously hypertensive rats (SHR) were investigated. We demonstrated that Allyl methyl sulphide (AMS) and Diallyl sulphide (DAS), inhibited AoSMC angiotensin II-stimulated cell cycle progression and migration. Neither cell viability nor annexin-V binding analysis revealed cytotoxic effects of both organosulphur compounds at the used concentrations. Instead, their inhibitory effects were associated to the prevention of the cell cycle inhibitor p27Kip1 (p27) downregulation and the reduction of ERK 1/2 phosphorilation. When we assessed the antioxidant activity of AMS and DAS we found that both organosulphur compounds inhibited angiotensin II- reactive oxygen species (ROS) generation. Our findings show that AMS and DAS, compounds derivate from garlic, could be effective antioxidants targeted at the arterial remodeling seen in hypertension.Fil: Castro, Claudia Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Gil Lorenzo, Andrea Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Gonzalez, Adriana. Universidad Nacional de Cuyo; ArgentinaFil: Cruzado, Montserrat Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaWiley VCH Verlag2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280059Castro, Claudia Magdalena; Gil Lorenzo, Andrea Fernanda; Gonzalez, Adriana; Cruzado, Montserrat Cecilia; Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase; Wiley VCH Verlag; Molecular Nutrition & Food Research; 54; 6; 12-2010; 781-7871613-4125CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/mnfr.200900108info:eu-repo/semantics/altIdentifier/doi/10.1002/mnfr.200900108info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:30:46Zoai:ri.conicet.gov.ar:11336/280059instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:30:46.433CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase
title Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase
spellingShingle Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase
Castro, Claudia Magdalena
ANGIOTENSIN II
CELL CYCLE
GARLIC
HYPERTENSION
title_short Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase
title_full Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase
title_fullStr Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase
title_full_unstemmed Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase
title_sort Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase
dc.creator.none.fl_str_mv Castro, Claudia Magdalena
Gil Lorenzo, Andrea Fernanda
Gonzalez, Adriana
Cruzado, Montserrat Cecilia
author Castro, Claudia Magdalena
author_facet Castro, Claudia Magdalena
Gil Lorenzo, Andrea Fernanda
Gonzalez, Adriana
Cruzado, Montserrat Cecilia
author_role author
author2 Gil Lorenzo, Andrea Fernanda
Gonzalez, Adriana
Cruzado, Montserrat Cecilia
author2_role author
author
author
dc.subject.none.fl_str_mv ANGIOTENSIN II
CELL CYCLE
GARLIC
HYPERTENSION
topic ANGIOTENSIN II
CELL CYCLE
GARLIC
HYPERTENSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Garlic has been used for prevention and treatment of hypertension; however the molecular mechanisms of garlic’s effects remain to be elucidated. In the present study, the mechanisms of the in vitro effect of organosulphur compounds derived from garlic on growth and migration of cultured aortic smooth muscle cells (AoSMC) isolated from spontaneously hypertensive rats (SHR) were investigated. We demonstrated that Allyl methyl sulphide (AMS) and Diallyl sulphide (DAS), inhibited AoSMC angiotensin II-stimulated cell cycle progression and migration. Neither cell viability nor annexin-V binding analysis revealed cytotoxic effects of both organosulphur compounds at the used concentrations. Instead, their inhibitory effects were associated to the prevention of the cell cycle inhibitor p27Kip1 (p27) downregulation and the reduction of ERK 1/2 phosphorilation. When we assessed the antioxidant activity of AMS and DAS we found that both organosulphur compounds inhibited angiotensin II- reactive oxygen species (ROS) generation. Our findings show that AMS and DAS, compounds derivate from garlic, could be effective antioxidants targeted at the arterial remodeling seen in hypertension.
Fil: Castro, Claudia Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Gil Lorenzo, Andrea Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Gonzalez, Adriana. Universidad Nacional de Cuyo; Argentina
Fil: Cruzado, Montserrat Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description Garlic has been used for prevention and treatment of hypertension; however the molecular mechanisms of garlic’s effects remain to be elucidated. In the present study, the mechanisms of the in vitro effect of organosulphur compounds derived from garlic on growth and migration of cultured aortic smooth muscle cells (AoSMC) isolated from spontaneously hypertensive rats (SHR) were investigated. We demonstrated that Allyl methyl sulphide (AMS) and Diallyl sulphide (DAS), inhibited AoSMC angiotensin II-stimulated cell cycle progression and migration. Neither cell viability nor annexin-V binding analysis revealed cytotoxic effects of both organosulphur compounds at the used concentrations. Instead, their inhibitory effects were associated to the prevention of the cell cycle inhibitor p27Kip1 (p27) downregulation and the reduction of ERK 1/2 phosphorilation. When we assessed the antioxidant activity of AMS and DAS we found that both organosulphur compounds inhibited angiotensin II- reactive oxygen species (ROS) generation. Our findings show that AMS and DAS, compounds derivate from garlic, could be effective antioxidants targeted at the arterial remodeling seen in hypertension.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280059
Castro, Claudia Magdalena; Gil Lorenzo, Andrea Fernanda; Gonzalez, Adriana; Cruzado, Montserrat Cecilia; Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase; Wiley VCH Verlag; Molecular Nutrition & Food Research; 54; 6; 12-2010; 781-787
1613-4125
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280059
identifier_str_mv Castro, Claudia Magdalena; Gil Lorenzo, Andrea Fernanda; Gonzalez, Adriana; Cruzado, Montserrat Cecilia; Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27 Kip1 and mitogen‐activated protein kinase; Wiley VCH Verlag; Molecular Nutrition & Food Research; 54; 6; 12-2010; 781-787
1613-4125
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/mnfr.200900108
info:eu-repo/semantics/altIdentifier/doi/10.1002/mnfr.200900108
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.665996

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