Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane

Autores
Arriola Benitez, Paula Constanza; Liotta, Domingo Javier; Padovan, C.; Quiroga, Marina Inés
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leaf scald disease caused by Xanthomonas albilineans (Ashby) Dowson is one of the most devastating diseases of sugarcane which has a direct impact on production and manufacturing quality. Currently, molecular tests have been proposed for the detection of this phytopathogen. In this work, two PCR's protocols developed by Pan et al. in 1997 and 1999 were evaluated, with primers that amplify a fragment of the ITS region between the ribosomal genes 16S and 23S. The search of X. albilineans was done from the juice obtained of 35 asymptomatic and symptomatic plants of sugarcane cultivated in localities of the provinces of Misiones and Corrientes, Argentina. X. albilineans was detected in only one of the symptomatic samples but in any of the asymptomatic ones. The protocol developed in 1999 was the one that gave the best results. It is important to emphasize the detection of this phytopathogen in the province of Misiones.
Fil: Arriola Benitez, Paula Constanza. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Padovan, C.. Programa de Desarrollo de Áreas Rurales; Argentina
Fil: Quiroga, Marina Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Materia
Phytopathogen
Molecular Detection
Sugarcane Diseases
Leaf Scald
Xanthomonas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18742
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcaneArriola Benitez, Paula ConstanzaLiotta, Domingo JavierPadovan, C.Quiroga, Marina InésPhytopathogenMolecular DetectionSugarcane DiseasesLeaf ScaldXanthomonashttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Leaf scald disease caused by Xanthomonas albilineans (Ashby) Dowson is one of the most devastating diseases of sugarcane which has a direct impact on production and manufacturing quality. Currently, molecular tests have been proposed for the detection of this phytopathogen. In this work, two PCR's protocols developed by Pan et al. in 1997 and 1999 were evaluated, with primers that amplify a fragment of the ITS region between the ribosomal genes 16S and 23S. The search of X. albilineans was done from the juice obtained of 35 asymptomatic and symptomatic plants of sugarcane cultivated in localities of the provinces of Misiones and Corrientes, Argentina. X. albilineans was detected in only one of the symptomatic samples but in any of the asymptomatic ones. The protocol developed in 1999 was the one that gave the best results. It is important to emphasize the detection of this phytopathogen in the province of Misiones.Fil: Arriola Benitez, Paula Constanza. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Padovan, C.. Programa de Desarrollo de Áreas Rurales; ArgentinaFil: Quiroga, Marina Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; ArgentinaOpen Science2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18742Arriola Benitez, Paula Constanza; Liotta, Domingo Javier; Padovan, C.; Quiroga, Marina Inés; Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane; Open Science; International Journal of Agriculture, Forestry and Fisheries; 3; 1; 2-2015; 12-162381-43652381-4373CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.openscienceonline.com/journal/archive2?journalId=706&paperId=1434info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:26:23Zoai:ri.conicet.gov.ar:11336/18742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:26:23.767CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane
title Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane
spellingShingle Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane
Arriola Benitez, Paula Constanza
Phytopathogen
Molecular Detection
Sugarcane Diseases
Leaf Scald
Xanthomonas
title_short Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane
title_full Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane
title_fullStr Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane
title_full_unstemmed Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane
title_sort Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane
dc.creator.none.fl_str_mv Arriola Benitez, Paula Constanza
Liotta, Domingo Javier
Padovan, C.
Quiroga, Marina Inés
author Arriola Benitez, Paula Constanza
author_facet Arriola Benitez, Paula Constanza
Liotta, Domingo Javier
Padovan, C.
Quiroga, Marina Inés
author_role author
author2 Liotta, Domingo Javier
Padovan, C.
Quiroga, Marina Inés
author2_role author
author
author
dc.subject.none.fl_str_mv Phytopathogen
Molecular Detection
Sugarcane Diseases
Leaf Scald
Xanthomonas
topic Phytopathogen
Molecular Detection
Sugarcane Diseases
Leaf Scald
Xanthomonas
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Leaf scald disease caused by Xanthomonas albilineans (Ashby) Dowson is one of the most devastating diseases of sugarcane which has a direct impact on production and manufacturing quality. Currently, molecular tests have been proposed for the detection of this phytopathogen. In this work, two PCR's protocols developed by Pan et al. in 1997 and 1999 were evaluated, with primers that amplify a fragment of the ITS region between the ribosomal genes 16S and 23S. The search of X. albilineans was done from the juice obtained of 35 asymptomatic and symptomatic plants of sugarcane cultivated in localities of the provinces of Misiones and Corrientes, Argentina. X. albilineans was detected in only one of the symptomatic samples but in any of the asymptomatic ones. The protocol developed in 1999 was the one that gave the best results. It is important to emphasize the detection of this phytopathogen in the province of Misiones.
Fil: Arriola Benitez, Paula Constanza. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Padovan, C.. Programa de Desarrollo de Áreas Rurales; Argentina
Fil: Quiroga, Marina Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
description Leaf scald disease caused by Xanthomonas albilineans (Ashby) Dowson is one of the most devastating diseases of sugarcane which has a direct impact on production and manufacturing quality. Currently, molecular tests have been proposed for the detection of this phytopathogen. In this work, two PCR's protocols developed by Pan et al. in 1997 and 1999 were evaluated, with primers that amplify a fragment of the ITS region between the ribosomal genes 16S and 23S. The search of X. albilineans was done from the juice obtained of 35 asymptomatic and symptomatic plants of sugarcane cultivated in localities of the provinces of Misiones and Corrientes, Argentina. X. albilineans was detected in only one of the symptomatic samples but in any of the asymptomatic ones. The protocol developed in 1999 was the one that gave the best results. It is important to emphasize the detection of this phytopathogen in the province of Misiones.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18742
Arriola Benitez, Paula Constanza; Liotta, Domingo Javier; Padovan, C.; Quiroga, Marina Inés; Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane; Open Science; International Journal of Agriculture, Forestry and Fisheries; 3; 1; 2-2015; 12-16
2381-4365
2381-4373
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18742
identifier_str_mv Arriola Benitez, Paula Constanza; Liotta, Domingo Javier; Padovan, C.; Quiroga, Marina Inés; Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane; Open Science; International Journal of Agriculture, Forestry and Fisheries; 3; 1; 2-2015; 12-16
2381-4365
2381-4373
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.openscienceonline.com/journal/archive2?journalId=706&paperId=1434
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Open Science
publisher.none.fl_str_mv Open Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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