Chitosan enhances transcellular permeability in human and rat intestine epithelium

Autores
Canali, María Magdalena; Pedrotti, Luciano Pablo; Balsinde, Jesús; Ibarra, Cristina Adriana; Correa, Silvia Graciela
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The intestinal epithelium regulates the transit of molecules from and into the organism. Several agentsact as absorption enhancers inducing changes in both transcellular and paracellular routes. Chitosan isa non-toxic biocompatible polysaccharide widely used as dietary supplement and mucosal delivery.Chitosan triggers both the activation of intestinal epithelial cells and the release of regulatory factors relevantfor its immunomodulatory activity. Yet, the interaction of chitosan with intestinal epithelial cells ispoorly characterized. We studied the uptake of this polysaccharide, and we evaluated its effects in boththe net water and ion movements across human and rat colon samples and the epithelial permeability.Herein, we demonstrate that chitosan increases the transcellular permeability to ions, water and proteinmarkers in human and rat intestinal mucosa and decreases the water permeability across the paracellularpathway. These findings are relevant to understand the activity of the polysaccharide in the mucosalenvironment.
Fil: Canali, María Magdalena. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pedrotti, Luciano Pablo. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Balsinde, Jesús. Universidad de Valladolid; España
Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Correa, Silvia Graciela. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ANTIGEN
CHITOSAN
ELECTROPHYSIOLOGY
INTESTINE
WATER TRANSPORT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/198942
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/198942
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chitosan enhances transcellular permeability in human and rat intestine epitheliumCanali, María MagdalenaPedrotti, Luciano PabloBalsinde, JesúsIbarra, Cristina AdrianaCorrea, Silvia GracielaANTIGENCHITOSANELECTROPHYSIOLOGYINTESTINEWATER TRANSPORThttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The intestinal epithelium regulates the transit of molecules from and into the organism. Several agentsact as absorption enhancers inducing changes in both transcellular and paracellular routes. Chitosan isa non-toxic biocompatible polysaccharide widely used as dietary supplement and mucosal delivery.Chitosan triggers both the activation of intestinal epithelial cells and the release of regulatory factors relevantfor its immunomodulatory activity. Yet, the interaction of chitosan with intestinal epithelial cells ispoorly characterized. We studied the uptake of this polysaccharide, and we evaluated its effects in boththe net water and ion movements across human and rat colon samples and the epithelial permeability.Herein, we demonstrate that chitosan increases the transcellular permeability to ions, water and proteinmarkers in human and rat intestinal mucosa and decreases the water permeability across the paracellularpathway. These findings are relevant to understand the activity of the polysaccharide in the mucosalenvironment.Fil: Canali, María Magdalena. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pedrotti, Luciano Pablo. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Balsinde, Jesús. Universidad de Valladolid; EspañaFil: Ibarra, Cristina Adriana. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Correa, Silvia Graciela. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/198942Canali, María Magdalena; Pedrotti, Luciano Pablo; Balsinde, Jesús; Ibarra, Cristina Adriana; Correa, Silvia Graciela; Chitosan enhances transcellular permeability in human and rat intestine epithelium; Elsevier Science; European Journal of Pharmaceutics and Biopharmaceutics; 80; 2; 2-2012; 418-4250939-6411CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0939641111003250info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejpb.2011.11.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:17Zoai:ri.conicet.gov.ar:11336/198942instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:17.722CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chitosan enhances transcellular permeability in human and rat intestine epithelium
title Chitosan enhances transcellular permeability in human and rat intestine epithelium
spellingShingle Chitosan enhances transcellular permeability in human and rat intestine epithelium
Canali, María Magdalena
ANTIGEN
CHITOSAN
ELECTROPHYSIOLOGY
INTESTINE
WATER TRANSPORT
title_short Chitosan enhances transcellular permeability in human and rat intestine epithelium
title_full Chitosan enhances transcellular permeability in human and rat intestine epithelium
title_fullStr Chitosan enhances transcellular permeability in human and rat intestine epithelium
title_full_unstemmed Chitosan enhances transcellular permeability in human and rat intestine epithelium
title_sort Chitosan enhances transcellular permeability in human and rat intestine epithelium
dc.creator.none.fl_str_mv Canali, María Magdalena
Pedrotti, Luciano Pablo
Balsinde, Jesús
Ibarra, Cristina Adriana
Correa, Silvia Graciela
author Canali, María Magdalena
author_facet Canali, María Magdalena
Pedrotti, Luciano Pablo
Balsinde, Jesús
Ibarra, Cristina Adriana
Correa, Silvia Graciela
author_role author
author2 Pedrotti, Luciano Pablo
Balsinde, Jesús
Ibarra, Cristina Adriana
Correa, Silvia Graciela
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTIGEN
CHITOSAN
ELECTROPHYSIOLOGY
INTESTINE
WATER TRANSPORT
topic ANTIGEN
CHITOSAN
ELECTROPHYSIOLOGY
INTESTINE
WATER TRANSPORT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The intestinal epithelium regulates the transit of molecules from and into the organism. Several agentsact as absorption enhancers inducing changes in both transcellular and paracellular routes. Chitosan isa non-toxic biocompatible polysaccharide widely used as dietary supplement and mucosal delivery.Chitosan triggers both the activation of intestinal epithelial cells and the release of regulatory factors relevantfor its immunomodulatory activity. Yet, the interaction of chitosan with intestinal epithelial cells ispoorly characterized. We studied the uptake of this polysaccharide, and we evaluated its effects in boththe net water and ion movements across human and rat colon samples and the epithelial permeability.Herein, we demonstrate that chitosan increases the transcellular permeability to ions, water and proteinmarkers in human and rat intestinal mucosa and decreases the water permeability across the paracellularpathway. These findings are relevant to understand the activity of the polysaccharide in the mucosalenvironment.
Fil: Canali, María Magdalena. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pedrotti, Luciano Pablo. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Balsinde, Jesús. Universidad de Valladolid; España
Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Correa, Silvia Graciela. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The intestinal epithelium regulates the transit of molecules from and into the organism. Several agentsact as absorption enhancers inducing changes in both transcellular and paracellular routes. Chitosan isa non-toxic biocompatible polysaccharide widely used as dietary supplement and mucosal delivery.Chitosan triggers both the activation of intestinal epithelial cells and the release of regulatory factors relevantfor its immunomodulatory activity. Yet, the interaction of chitosan with intestinal epithelial cells ispoorly characterized. We studied the uptake of this polysaccharide, and we evaluated its effects in boththe net water and ion movements across human and rat colon samples and the epithelial permeability.Herein, we demonstrate that chitosan increases the transcellular permeability to ions, water and proteinmarkers in human and rat intestinal mucosa and decreases the water permeability across the paracellularpathway. These findings are relevant to understand the activity of the polysaccharide in the mucosalenvironment.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/198942
Canali, María Magdalena; Pedrotti, Luciano Pablo; Balsinde, Jesús; Ibarra, Cristina Adriana; Correa, Silvia Graciela; Chitosan enhances transcellular permeability in human and rat intestine epithelium; Elsevier Science; European Journal of Pharmaceutics and Biopharmaceutics; 80; 2; 2-2012; 418-425
0939-6411
CONICET Digital
CONICET
url http://hdl.handle.net/11336/198942
identifier_str_mv Canali, María Magdalena; Pedrotti, Luciano Pablo; Balsinde, Jesús; Ibarra, Cristina Adriana; Correa, Silvia Graciela; Chitosan enhances transcellular permeability in human and rat intestine epithelium; Elsevier Science; European Journal of Pharmaceutics and Biopharmaceutics; 80; 2; 2-2012; 418-425
0939-6411
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0939641111003250
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejpb.2011.11.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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