Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin

Autores
Adriano, Wellington; Mendonça, Dany; Rodrigues, Dasciana; Mammarella, Enrique José; Giordano, Raquel
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Changing gel structure and immobilization conditions led to a significant improvement in the covalent multipoint attachment of chymotrypsin on chitosan. The use of sodium alginate, gelatin, or κ-carrageenan, activation with glutaraldehyde, glycidol, or epichlorohydrin, and addition of microorganisms followed by cellular lysis allowed the modification of the gel structure. Immobilization yields, recovered activities, and stabilization factors at 55 and 65 °C were evaluated. Enzyme immobilization for 72 h at pH 10.05, 25 °C and reduction with NaBH4 in chitosan 2.5%−carrageenan 2.5%, with addition of S. cerevisiae 5% and activation with epichlorohydrin led to the best derivative, which was 9900-fold more stable than the soluble enzyme. This support allowed an enzyme load up to 40 mg chymotrypsin × ggel−1. The number of covalent bonds, formed by active groups in the support and lysine residues of the enzyme, can explain the obtained results. SEM images of the gel structures corroborate these conclusions.
Fil: Adriano, Wellington. Universidade Federal do Sao Carlos; Brasil
Fil: Mendonça, Dany. Universidade Federal do Sao Carlos; Brasil
Fil: Rodrigues, Dasciana. Universidade Federal do Sao Carlos; Brasil
Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Giordano, Raquel. Universidade Federal do Sao Carlos; Brasil
Materia
Covalent Multipoint Immobilization
Chymotrypsin
Chitosan
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19798
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19798
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsinAdriano, WellingtonMendonça, DanyRodrigues, DascianaMammarella, Enrique JoséGiordano, RaquelCovalent Multipoint ImmobilizationChymotrypsinChitosanhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Changing gel structure and immobilization conditions led to a significant improvement in the covalent multipoint attachment of chymotrypsin on chitosan. The use of sodium alginate, gelatin, or κ-carrageenan, activation with glutaraldehyde, glycidol, or epichlorohydrin, and addition of microorganisms followed by cellular lysis allowed the modification of the gel structure. Immobilization yields, recovered activities, and stabilization factors at 55 and 65 °C were evaluated. Enzyme immobilization for 72 h at pH 10.05, 25 °C and reduction with NaBH4 in chitosan 2.5%−carrageenan 2.5%, with addition of S. cerevisiae 5% and activation with epichlorohydrin led to the best derivative, which was 9900-fold more stable than the soluble enzyme. This support allowed an enzyme load up to 40 mg chymotrypsin × ggel−1. The number of covalent bonds, formed by active groups in the support and lysine residues of the enzyme, can explain the obtained results. SEM images of the gel structures corroborate these conclusions.Fil: Adriano, Wellington. Universidade Federal do Sao Carlos; BrasilFil: Mendonça, Dany. Universidade Federal do Sao Carlos; BrasilFil: Rodrigues, Dasciana. Universidade Federal do Sao Carlos; BrasilFil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Giordano, Raquel. Universidade Federal do Sao Carlos; BrasilAmerican Chemical Society2008-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19798Adriano, Wellington; Mendonça, Dany; Rodrigues, Dasciana; Mammarella, Enrique José; Giordano, Raquel; Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin; American Chemical Society; Biomacromolecules; 9; 8; 7-2008; 2170-21791525-7797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/bm8002754info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/bm8002754info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:24Zoai:ri.conicet.gov.ar:11336/19798instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:24.56CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
title Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
spellingShingle Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
Adriano, Wellington
Covalent Multipoint Immobilization
Chymotrypsin
Chitosan
title_short Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
title_full Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
title_fullStr Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
title_full_unstemmed Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
title_sort Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
dc.creator.none.fl_str_mv Adriano, Wellington
Mendonça, Dany
Rodrigues, Dasciana
Mammarella, Enrique José
Giordano, Raquel
author Adriano, Wellington
author_facet Adriano, Wellington
Mendonça, Dany
Rodrigues, Dasciana
Mammarella, Enrique José
Giordano, Raquel
author_role author
author2 Mendonça, Dany
Rodrigues, Dasciana
Mammarella, Enrique José
Giordano, Raquel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Covalent Multipoint Immobilization
Chymotrypsin
Chitosan
topic Covalent Multipoint Immobilization
Chymotrypsin
Chitosan
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Changing gel structure and immobilization conditions led to a significant improvement in the covalent multipoint attachment of chymotrypsin on chitosan. The use of sodium alginate, gelatin, or κ-carrageenan, activation with glutaraldehyde, glycidol, or epichlorohydrin, and addition of microorganisms followed by cellular lysis allowed the modification of the gel structure. Immobilization yields, recovered activities, and stabilization factors at 55 and 65 °C were evaluated. Enzyme immobilization for 72 h at pH 10.05, 25 °C and reduction with NaBH4 in chitosan 2.5%−carrageenan 2.5%, with addition of S. cerevisiae 5% and activation with epichlorohydrin led to the best derivative, which was 9900-fold more stable than the soluble enzyme. This support allowed an enzyme load up to 40 mg chymotrypsin × ggel−1. The number of covalent bonds, formed by active groups in the support and lysine residues of the enzyme, can explain the obtained results. SEM images of the gel structures corroborate these conclusions.
Fil: Adriano, Wellington. Universidade Federal do Sao Carlos; Brasil
Fil: Mendonça, Dany. Universidade Federal do Sao Carlos; Brasil
Fil: Rodrigues, Dasciana. Universidade Federal do Sao Carlos; Brasil
Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Giordano, Raquel. Universidade Federal do Sao Carlos; Brasil
description Changing gel structure and immobilization conditions led to a significant improvement in the covalent multipoint attachment of chymotrypsin on chitosan. The use of sodium alginate, gelatin, or κ-carrageenan, activation with glutaraldehyde, glycidol, or epichlorohydrin, and addition of microorganisms followed by cellular lysis allowed the modification of the gel structure. Immobilization yields, recovered activities, and stabilization factors at 55 and 65 °C were evaluated. Enzyme immobilization for 72 h at pH 10.05, 25 °C and reduction with NaBH4 in chitosan 2.5%−carrageenan 2.5%, with addition of S. cerevisiae 5% and activation with epichlorohydrin led to the best derivative, which was 9900-fold more stable than the soluble enzyme. This support allowed an enzyme load up to 40 mg chymotrypsin × ggel−1. The number of covalent bonds, formed by active groups in the support and lysine residues of the enzyme, can explain the obtained results. SEM images of the gel structures corroborate these conclusions.
publishDate 2008
dc.date.none.fl_str_mv 2008-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19798
Adriano, Wellington; Mendonça, Dany; Rodrigues, Dasciana; Mammarella, Enrique José; Giordano, Raquel; Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin; American Chemical Society; Biomacromolecules; 9; 8; 7-2008; 2170-2179
1525-7797
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19798
identifier_str_mv Adriano, Wellington; Mendonça, Dany; Rodrigues, Dasciana; Mammarella, Enrique José; Giordano, Raquel; Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin; American Chemical Society; Biomacromolecules; 9; 8; 7-2008; 2170-2179
1525-7797
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/bm8002754
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/bm8002754
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268599696228352
score 13.13397

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