Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry...

Autores
Mohedano, Mari Luz; Hernández Recio, Sara; Yépez, Alba; Requena, Teresa; Martínez Cuesta, M. Carmen; Peláez, Carmen; Russo, Pasquale; Leblanc, Jean Guy Joseph; Spano, Giuseppe; Aznar, Rosa; López, Paloma
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some strains of lactic acid bacteria (LAB) produce riboflavin, a water-soluble vitamin of the B complex, essential for human beings. Here, we have evaluated riboflavin (B2 vitamin) production by five Lactobacillus plantarum strains isolated from chicha, a traditional maize-based fermented alcoholic beverage from north-western Argentina and their isogenic riboflavin-overproducing derivatives previously selected using roseoflavin. A direct fluorescence spectroscopic detection method to quantify riboflavin production in bacterial culture supernatants has been tested. Comparison of the efficiency for riboflavin fluorescence quantification with and without prior HPLC fractionation showed that the developed method is a rapid and easy test for selection of B2 vitamin-producing strains. In addition, it can be used for quantitative detection of the vitamin production in real time during bacterial growth. On the basis of this and previous analyses, the L. plantarum M5MA1-B2 riboflavin overproducer was selected for in vitro and in vivo studies after being fluorescently labeled by transfer of the pRCR12 plasmid, which encodes the mCherry protein. The labeling did not affect negatively the growth, the riboflavin production nor the adhesion of the strain to Caco-2 cells. Thus, L. plantarum M5MA1-B2[pRCR12] was evaluated for its survival under digestive tract stresses in the presence of microbiota in the dynamic multistage BFBL gut model and in a murine model. After exposure to both models, M5MA1-B2[pRCR12] could be recovered and detected by the pink color of the colonies. The results indicated a satisfactory resistance of the strain to gastric and intestinal stress conditions but a low colonization capability observed both in vitro and in vivo. Overall, L. plantarum M5MA1-B2 could be proposed as a probiotic strain for the development of functional foods.
Fil: Mohedano, Mari Luz. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Hernández Recio, Sara. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Yépez, Alba. Universidad de Valencia; España
Fil: Requena, Teresa. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Martínez Cuesta, M. Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Peláez, Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Russo, Pasquale. Università di Foggia; Italia
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Spano, Giuseppe. Università di Foggia; Italia
Fil: Aznar, Rosa. Universidad de Valencia; España. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España
Fil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Materia
FLUORESCENT LABELING
LACTIC ACID BACTERIA
LACTOBACILLUS PLANTARUM
PROBIOTIC
RIBOFLAVIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96617
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labelingMohedano, Mari LuzHernández Recio, SaraYépez, AlbaRequena, TeresaMartínez Cuesta, M. CarmenPeláez, CarmenRusso, PasqualeLeblanc, Jean Guy JosephSpano, GiuseppeAznar, RosaLópez, PalomaFLUORESCENT LABELINGLACTIC ACID BACTERIALACTOBACILLUS PLANTARUMPROBIOTICRIBOFLAVINhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Some strains of lactic acid bacteria (LAB) produce riboflavin, a water-soluble vitamin of the B complex, essential for human beings. Here, we have evaluated riboflavin (B2 vitamin) production by five Lactobacillus plantarum strains isolated from chicha, a traditional maize-based fermented alcoholic beverage from north-western Argentina and their isogenic riboflavin-overproducing derivatives previously selected using roseoflavin. A direct fluorescence spectroscopic detection method to quantify riboflavin production in bacterial culture supernatants has been tested. Comparison of the efficiency for riboflavin fluorescence quantification with and without prior HPLC fractionation showed that the developed method is a rapid and easy test for selection of B2 vitamin-producing strains. In addition, it can be used for quantitative detection of the vitamin production in real time during bacterial growth. On the basis of this and previous analyses, the L. plantarum M5MA1-B2 riboflavin overproducer was selected for in vitro and in vivo studies after being fluorescently labeled by transfer of the pRCR12 plasmid, which encodes the mCherry protein. The labeling did not affect negatively the growth, the riboflavin production nor the adhesion of the strain to Caco-2 cells. Thus, L. plantarum M5MA1-B2[pRCR12] was evaluated for its survival under digestive tract stresses in the presence of microbiota in the dynamic multistage BFBL gut model and in a murine model. After exposure to both models, M5MA1-B2[pRCR12] could be recovered and detected by the pink color of the colonies. The results indicated a satisfactory resistance of the strain to gastric and intestinal stress conditions but a low colonization capability observed both in vitro and in vivo. Overall, L. plantarum M5MA1-B2 could be proposed as a probiotic strain for the development of functional foods.Fil: Mohedano, Mari Luz. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Hernández Recio, Sara. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Yépez, Alba. Universidad de Valencia; EspañaFil: Requena, Teresa. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; EspañaFil: Martínez Cuesta, M. Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; EspañaFil: Peláez, Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; EspañaFil: Russo, Pasquale. Università di Foggia; ItaliaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Spano, Giuseppe. Università di Foggia; ItaliaFil: Aznar, Rosa. Universidad de Valencia; España. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFrontiers Media S.A.2019-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96617Mohedano, Mari Luz; Hernández Recio, Sara; Yépez, Alba; Requena, Teresa; Martínez Cuesta, M. Carmen; et al.; Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling; Frontiers Media S.A.; Frontiers in Microbiology; 10; 31-7-2019; 1-131664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.01748/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.01748info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:14Zoai:ri.conicet.gov.ar:11336/96617instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:14.427CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling
title Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling
spellingShingle Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling
Mohedano, Mari Luz
FLUORESCENT LABELING
LACTIC ACID BACTERIA
LACTOBACILLUS PLANTARUM
PROBIOTIC
RIBOFLAVIN
title_short Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling
title_full Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling
title_fullStr Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling
title_full_unstemmed Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling
title_sort Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling
dc.creator.none.fl_str_mv Mohedano, Mari Luz
Hernández Recio, Sara
Yépez, Alba
Requena, Teresa
Martínez Cuesta, M. Carmen
Peláez, Carmen
Russo, Pasquale
Leblanc, Jean Guy Joseph
Spano, Giuseppe
Aznar, Rosa
López, Paloma
author Mohedano, Mari Luz
author_facet Mohedano, Mari Luz
Hernández Recio, Sara
Yépez, Alba
Requena, Teresa
Martínez Cuesta, M. Carmen
Peláez, Carmen
Russo, Pasquale
Leblanc, Jean Guy Joseph
Spano, Giuseppe
Aznar, Rosa
López, Paloma
author_role author
author2 Hernández Recio, Sara
Yépez, Alba
Requena, Teresa
Martínez Cuesta, M. Carmen
Peláez, Carmen
Russo, Pasquale
Leblanc, Jean Guy Joseph
Spano, Giuseppe
Aznar, Rosa
López, Paloma
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FLUORESCENT LABELING
LACTIC ACID BACTERIA
LACTOBACILLUS PLANTARUM
PROBIOTIC
RIBOFLAVIN
topic FLUORESCENT LABELING
LACTIC ACID BACTERIA
LACTOBACILLUS PLANTARUM
PROBIOTIC
RIBOFLAVIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Some strains of lactic acid bacteria (LAB) produce riboflavin, a water-soluble vitamin of the B complex, essential for human beings. Here, we have evaluated riboflavin (B2 vitamin) production by five Lactobacillus plantarum strains isolated from chicha, a traditional maize-based fermented alcoholic beverage from north-western Argentina and their isogenic riboflavin-overproducing derivatives previously selected using roseoflavin. A direct fluorescence spectroscopic detection method to quantify riboflavin production in bacterial culture supernatants has been tested. Comparison of the efficiency for riboflavin fluorescence quantification with and without prior HPLC fractionation showed that the developed method is a rapid and easy test for selection of B2 vitamin-producing strains. In addition, it can be used for quantitative detection of the vitamin production in real time during bacterial growth. On the basis of this and previous analyses, the L. plantarum M5MA1-B2 riboflavin overproducer was selected for in vitro and in vivo studies after being fluorescently labeled by transfer of the pRCR12 plasmid, which encodes the mCherry protein. The labeling did not affect negatively the growth, the riboflavin production nor the adhesion of the strain to Caco-2 cells. Thus, L. plantarum M5MA1-B2[pRCR12] was evaluated for its survival under digestive tract stresses in the presence of microbiota in the dynamic multistage BFBL gut model and in a murine model. After exposure to both models, M5MA1-B2[pRCR12] could be recovered and detected by the pink color of the colonies. The results indicated a satisfactory resistance of the strain to gastric and intestinal stress conditions but a low colonization capability observed both in vitro and in vivo. Overall, L. plantarum M5MA1-B2 could be proposed as a probiotic strain for the development of functional foods.
Fil: Mohedano, Mari Luz. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Hernández Recio, Sara. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Yépez, Alba. Universidad de Valencia; España
Fil: Requena, Teresa. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Martínez Cuesta, M. Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Peláez, Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Russo, Pasquale. Università di Foggia; Italia
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Spano, Giuseppe. Università di Foggia; Italia
Fil: Aznar, Rosa. Universidad de Valencia; España. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España
Fil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
description Some strains of lactic acid bacteria (LAB) produce riboflavin, a water-soluble vitamin of the B complex, essential for human beings. Here, we have evaluated riboflavin (B2 vitamin) production by five Lactobacillus plantarum strains isolated from chicha, a traditional maize-based fermented alcoholic beverage from north-western Argentina and their isogenic riboflavin-overproducing derivatives previously selected using roseoflavin. A direct fluorescence spectroscopic detection method to quantify riboflavin production in bacterial culture supernatants has been tested. Comparison of the efficiency for riboflavin fluorescence quantification with and without prior HPLC fractionation showed that the developed method is a rapid and easy test for selection of B2 vitamin-producing strains. In addition, it can be used for quantitative detection of the vitamin production in real time during bacterial growth. On the basis of this and previous analyses, the L. plantarum M5MA1-B2 riboflavin overproducer was selected for in vitro and in vivo studies after being fluorescently labeled by transfer of the pRCR12 plasmid, which encodes the mCherry protein. The labeling did not affect negatively the growth, the riboflavin production nor the adhesion of the strain to Caco-2 cells. Thus, L. plantarum M5MA1-B2[pRCR12] was evaluated for its survival under digestive tract stresses in the presence of microbiota in the dynamic multistage BFBL gut model and in a murine model. After exposure to both models, M5MA1-B2[pRCR12] could be recovered and detected by the pink color of the colonies. The results indicated a satisfactory resistance of the strain to gastric and intestinal stress conditions but a low colonization capability observed both in vitro and in vivo. Overall, L. plantarum M5MA1-B2 could be proposed as a probiotic strain for the development of functional foods.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96617
Mohedano, Mari Luz; Hernández Recio, Sara; Yépez, Alba; Requena, Teresa; Martínez Cuesta, M. Carmen; et al.; Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling; Frontiers Media S.A.; Frontiers in Microbiology; 10; 31-7-2019; 1-13
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96617
identifier_str_mv Mohedano, Mari Luz; Hernández Recio, Sara; Yépez, Alba; Requena, Teresa; Martínez Cuesta, M. Carmen; et al.; Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling; Frontiers Media S.A.; Frontiers in Microbiology; 10; 31-7-2019; 1-13
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.01748/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.01748
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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