Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties

Autores
Rodríguez, María Cecilia; Matulewicz, Maria Cristina; Noseda, M. D.; Ducatti, D. R. B.; Leonardi, Patricia Ines
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Milled summer thalli of Gracilaria gracilis from Argentina were sequentially extracted with water at room temperature (RTW1-3), 70 C (W701-3) and 90 C (W901-2). Both W701 and W901 consisted of high molecular weight polysaccharides (ca. 540,000 Da), but polydispersity was higher for the major product W701 (yield, 72% of the recovered). Structural analyzes by methylation and 13C NMR spectroscopy revealed that W701 was mainly agarose. Alkaline treatment, together with structural analyzes, indicated a negligible proportion of precursor L-galactose 6-sulfate residues in this product, while they were clearly detected in the 13C NMR spectra of RTW2-3. The presence of floridean starch in W901 had an antagonistic effect on its gel strength, which resulted nearly three times lower than that of fraction W701. Ultrastructural observation by transmission electron microscopy showed that, after extraction with hot water, a partial loss of cell wall stratification and disorganization of the cuticle had occurred. Final cellular debris exhibited swelling in the microfibrillar component. After this first thorough study of the chemical composition and physical properties of the products of G. gracilis from Bahía Bustamante we conclude that a good quality agarose is obtained in high yield after extraction with water at 70 C without the requirement of alkaline pretreatment, which usually produces degradation of the polysaccharide.
Fil: Rodríguez, María Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Matulewicz, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Noseda, M. D.. Universidade Federal do Paraná; Brasil
Fil: Ducatti, D. R. B.. Universidade Federal do Paraná; Brasil
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
Agarose
Cell Wall
Gracilaria Gracilis
Structure Determination
Sulfated Galactans
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26245
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/26245
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical propertiesRodríguez, María CeciliaMatulewicz, Maria CristinaNoseda, M. D.Ducatti, D. R. B.Leonardi, Patricia InesAgaroseCell WallGracilaria GracilisStructure DeterminationSulfated Galactanshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Milled summer thalli of Gracilaria gracilis from Argentina were sequentially extracted with water at room temperature (RTW1-3), 70 C (W701-3) and 90 C (W901-2). Both W701 and W901 consisted of high molecular weight polysaccharides (ca. 540,000 Da), but polydispersity was higher for the major product W701 (yield, 72% of the recovered). Structural analyzes by methylation and 13C NMR spectroscopy revealed that W701 was mainly agarose. Alkaline treatment, together with structural analyzes, indicated a negligible proportion of precursor L-galactose 6-sulfate residues in this product, while they were clearly detected in the 13C NMR spectra of RTW2-3. The presence of floridean starch in W901 had an antagonistic effect on its gel strength, which resulted nearly three times lower than that of fraction W701. Ultrastructural observation by transmission electron microscopy showed that, after extraction with hot water, a partial loss of cell wall stratification and disorganization of the cuticle had occurred. Final cellular debris exhibited swelling in the microfibrillar component. After this first thorough study of the chemical composition and physical properties of the products of G. gracilis from Bahía Bustamante we conclude that a good quality agarose is obtained in high yield after extraction with water at 70 C without the requirement of alkaline pretreatment, which usually produces degradation of the polysaccharide.Fil: Rodríguez, María Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Matulewicz, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Noseda, M. D.. Universidade Federal do Paraná; BrasilFil: Ducatti, D. R. B.. Universidade Federal do Paraná; BrasilFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaElsevier2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26245Rodríguez, María Cecilia; Matulewicz, Maria Cristina; Noseda, M. D.; Ducatti, D. R. B.; Leonardi, Patricia Ines; Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties; Elsevier; Bioresource Technology; 100; 3; 2-2009; 1435-14410960-8524CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2008.08.025info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960852408007256info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:21Zoai:ri.conicet.gov.ar:11336/26245instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:21.715CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties
title Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties
spellingShingle Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties
Rodríguez, María Cecilia
Agarose
Cell Wall
Gracilaria Gracilis
Structure Determination
Sulfated Galactans
title_short Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties
title_full Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties
title_fullStr Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties
title_full_unstemmed Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties
title_sort Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties
dc.creator.none.fl_str_mv Rodríguez, María Cecilia
Matulewicz, Maria Cristina
Noseda, M. D.
Ducatti, D. R. B.
Leonardi, Patricia Ines
author Rodríguez, María Cecilia
author_facet Rodríguez, María Cecilia
Matulewicz, Maria Cristina
Noseda, M. D.
Ducatti, D. R. B.
Leonardi, Patricia Ines
author_role author
author2 Matulewicz, Maria Cristina
Noseda, M. D.
Ducatti, D. R. B.
Leonardi, Patricia Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv Agarose
Cell Wall
Gracilaria Gracilis
Structure Determination
Sulfated Galactans
topic Agarose
Cell Wall
Gracilaria Gracilis
Structure Determination
Sulfated Galactans
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Milled summer thalli of Gracilaria gracilis from Argentina were sequentially extracted with water at room temperature (RTW1-3), 70 C (W701-3) and 90 C (W901-2). Both W701 and W901 consisted of high molecular weight polysaccharides (ca. 540,000 Da), but polydispersity was higher for the major product W701 (yield, 72% of the recovered). Structural analyzes by methylation and 13C NMR spectroscopy revealed that W701 was mainly agarose. Alkaline treatment, together with structural analyzes, indicated a negligible proportion of precursor L-galactose 6-sulfate residues in this product, while they were clearly detected in the 13C NMR spectra of RTW2-3. The presence of floridean starch in W901 had an antagonistic effect on its gel strength, which resulted nearly three times lower than that of fraction W701. Ultrastructural observation by transmission electron microscopy showed that, after extraction with hot water, a partial loss of cell wall stratification and disorganization of the cuticle had occurred. Final cellular debris exhibited swelling in the microfibrillar component. After this first thorough study of the chemical composition and physical properties of the products of G. gracilis from Bahía Bustamante we conclude that a good quality agarose is obtained in high yield after extraction with water at 70 C without the requirement of alkaline pretreatment, which usually produces degradation of the polysaccharide.
Fil: Rodríguez, María Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Matulewicz, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Noseda, M. D.. Universidade Federal do Paraná; Brasil
Fil: Ducatti, D. R. B.. Universidade Federal do Paraná; Brasil
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description Milled summer thalli of Gracilaria gracilis from Argentina were sequentially extracted with water at room temperature (RTW1-3), 70 C (W701-3) and 90 C (W901-2). Both W701 and W901 consisted of high molecular weight polysaccharides (ca. 540,000 Da), but polydispersity was higher for the major product W701 (yield, 72% of the recovered). Structural analyzes by methylation and 13C NMR spectroscopy revealed that W701 was mainly agarose. Alkaline treatment, together with structural analyzes, indicated a negligible proportion of precursor L-galactose 6-sulfate residues in this product, while they were clearly detected in the 13C NMR spectra of RTW2-3. The presence of floridean starch in W901 had an antagonistic effect on its gel strength, which resulted nearly three times lower than that of fraction W701. Ultrastructural observation by transmission electron microscopy showed that, after extraction with hot water, a partial loss of cell wall stratification and disorganization of the cuticle had occurred. Final cellular debris exhibited swelling in the microfibrillar component. After this first thorough study of the chemical composition and physical properties of the products of G. gracilis from Bahía Bustamante we conclude that a good quality agarose is obtained in high yield after extraction with water at 70 C without the requirement of alkaline pretreatment, which usually produces degradation of the polysaccharide.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26245
Rodríguez, María Cecilia; Matulewicz, Maria Cristina; Noseda, M. D.; Ducatti, D. R. B.; Leonardi, Patricia Ines; Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties; Elsevier; Bioresource Technology; 100; 3; 2-2009; 1435-1441
0960-8524
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26245
identifier_str_mv Rodríguez, María Cecilia; Matulewicz, Maria Cristina; Noseda, M. D.; Ducatti, D. R. B.; Leonardi, Patricia Ines; Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina-Content, structure and physical properties; Elsevier; Bioresource Technology; 100; 3; 2-2009; 1435-1441
0960-8524
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2008.08.025
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960852408007256
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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