A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions

Autores
Sacca, Paula Alejandra; Pistone Creydt, Virginia; Tesone, Amelia Julieta; Calvo, Juan Carlos
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The goal of the present work was to obtain a simple and reproducible experimental model that would maintain the characteristics of the extracellular physiological environment of breast epithelial cells, both in factors as well as stromal structure, on which we could grow and evaluate changes of normal and tumor breast cells. 3T3-L1 pre-adipocytes (breast stromal cell model) were cultured and irradiated in a microwave oven at different times and potencies. In order to lose their proliferation ability, cells had to be irradiated twice at 650 Watts with a two-minute pulse each. The characteristics of the treated stromal support were analyzed for cell morphology, presence of DNA and proteins. We then evaluated on this support the effect on proliferation and migration of both normal and tumor - murine and human - breast epithelial cells. Both cell types increased their proliferation, while only tumor cells increased migration, thus improving their metastatic capacity. We believe this is a new and simple experimental method of studying epithelial-stromal cell interaction.
Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Tesone, Amelia Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
CANCER
EPITHELIAL-STROMAL INTERACTIONS
EXTRACELLULAR MATRIX
MICROWAVE OVEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26290
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel and alternative in vitro method using microwave to study the epithelial-stromal interactionsSacca, Paula AlejandraPistone Creydt, VirginiaTesone, Amelia JulietaCalvo, Juan CarlosCANCEREPITHELIAL-STROMAL INTERACTIONSEXTRACELLULAR MATRIXMICROWAVE OVENhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The goal of the present work was to obtain a simple and reproducible experimental model that would maintain the characteristics of the extracellular physiological environment of breast epithelial cells, both in factors as well as stromal structure, on which we could grow and evaluate changes of normal and tumor breast cells. 3T3-L1 pre-adipocytes (breast stromal cell model) were cultured and irradiated in a microwave oven at different times and potencies. In order to lose their proliferation ability, cells had to be irradiated twice at 650 Watts with a two-minute pulse each. The characteristics of the treated stromal support were analyzed for cell morphology, presence of DNA and proteins. We then evaluated on this support the effect on proliferation and migration of both normal and tumor - murine and human - breast epithelial cells. Both cell types increased their proliferation, while only tumor cells increased migration, thus improving their metastatic capacity. We believe this is a new and simple experimental method of studying epithelial-stromal cell interaction.Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Tesone, Amelia Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaAcademic Journals2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26290Sacca, Paula Alejandra; Pistone Creydt, Virginia; Tesone, Amelia Julieta; Calvo, Juan Carlos; A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions; Academic Journals; Scientific Research and Essay; 4; 12; 12-2009; 1487-14951992-22481992-2248CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.academicjournals.org/journal/SRE/article-abstract/E7782C819974info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:05:28Zoai:ri.conicet.gov.ar:11336/26290instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:05:28.56CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
title A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
spellingShingle A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
Sacca, Paula Alejandra
CANCER
EPITHELIAL-STROMAL INTERACTIONS
EXTRACELLULAR MATRIX
MICROWAVE OVEN
title_short A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
title_full A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
title_fullStr A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
title_full_unstemmed A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
title_sort A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
dc.creator.none.fl_str_mv Sacca, Paula Alejandra
Pistone Creydt, Virginia
Tesone, Amelia Julieta
Calvo, Juan Carlos
author Sacca, Paula Alejandra
author_facet Sacca, Paula Alejandra
Pistone Creydt, Virginia
Tesone, Amelia Julieta
Calvo, Juan Carlos
author_role author
author2 Pistone Creydt, Virginia
Tesone, Amelia Julieta
Calvo, Juan Carlos
author2_role author
author
author
dc.subject.none.fl_str_mv CANCER
EPITHELIAL-STROMAL INTERACTIONS
EXTRACELLULAR MATRIX
MICROWAVE OVEN
topic CANCER
EPITHELIAL-STROMAL INTERACTIONS
EXTRACELLULAR MATRIX
MICROWAVE OVEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The goal of the present work was to obtain a simple and reproducible experimental model that would maintain the characteristics of the extracellular physiological environment of breast epithelial cells, both in factors as well as stromal structure, on which we could grow and evaluate changes of normal and tumor breast cells. 3T3-L1 pre-adipocytes (breast stromal cell model) were cultured and irradiated in a microwave oven at different times and potencies. In order to lose their proliferation ability, cells had to be irradiated twice at 650 Watts with a two-minute pulse each. The characteristics of the treated stromal support were analyzed for cell morphology, presence of DNA and proteins. We then evaluated on this support the effect on proliferation and migration of both normal and tumor - murine and human - breast epithelial cells. Both cell types increased their proliferation, while only tumor cells increased migration, thus improving their metastatic capacity. We believe this is a new and simple experimental method of studying epithelial-stromal cell interaction.
Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Tesone, Amelia Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description The goal of the present work was to obtain a simple and reproducible experimental model that would maintain the characteristics of the extracellular physiological environment of breast epithelial cells, both in factors as well as stromal structure, on which we could grow and evaluate changes of normal and tumor breast cells. 3T3-L1 pre-adipocytes (breast stromal cell model) were cultured and irradiated in a microwave oven at different times and potencies. In order to lose their proliferation ability, cells had to be irradiated twice at 650 Watts with a two-minute pulse each. The characteristics of the treated stromal support were analyzed for cell morphology, presence of DNA and proteins. We then evaluated on this support the effect on proliferation and migration of both normal and tumor - murine and human - breast epithelial cells. Both cell types increased their proliferation, while only tumor cells increased migration, thus improving their metastatic capacity. We believe this is a new and simple experimental method of studying epithelial-stromal cell interaction.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26290
Sacca, Paula Alejandra; Pistone Creydt, Virginia; Tesone, Amelia Julieta; Calvo, Juan Carlos; A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions; Academic Journals; Scientific Research and Essay; 4; 12; 12-2009; 1487-1495
1992-2248
1992-2248
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26290
identifier_str_mv Sacca, Paula Alejandra; Pistone Creydt, Virginia; Tesone, Amelia Julieta; Calvo, Juan Carlos; A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions; Academic Journals; Scientific Research and Essay; 4; 12; 12-2009; 1487-1495
1992-2248
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.academicjournals.org/journal/SRE/article-abstract/E7782C819974
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781334268149760
score 12.982451

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