β3-Chimaerin, a novel member of the chimaerin Rac-GAP family

Autores
Zubeldia Brenner, Lautaro; Gutierrez Uzquiza, Alvaro; Barrio Real, Laura; Wang, Hongbin; Kazanietz, Marcelo Gabriel; Coluccio Leskow, Federico
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (β1- and β2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that β1- and β2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named β3-chimaerin. Expression profile analysis revealed predominantly low levels for the β3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, β3-chimaerin has a unique N-terminal domain. Studies in cells established that β3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of β3-chimaerin for phorbol ester-induced translocation relative to β2-chimaerin suggests differential ligand accessibility to the C1 domain.
Fil: Zubeldia Brenner, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gutierrez Uzquiza, Alvaro. University of Pennsylvania; Estados Unidos
Fil: Barrio Real, Laura. University of Pennsylvania; Estados Unidos
Fil: Wang, Hongbin. University of Pennsylvania; Estados Unidos
Fil: Kazanietz, Marcelo Gabriel. University of Pennsylvania; Estados Unidos
Fil: Coluccio Leskow, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Materia
Chimaerin
Rac
Gap
Tyrosine Kinase Receptor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32090
Acceder
id CONICETDig_b6e263135f27f6b974d7463e3479ff31
oai_identifier_str oai:ri.conicet.gov.ar:11336/32090
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling β3-Chimaerin, a novel member of the chimaerin Rac-GAP familyZubeldia Brenner, LautaroGutierrez Uzquiza, AlvaroBarrio Real, LauraWang, HongbinKazanietz, Marcelo GabrielColuccio Leskow, FedericoChimaerinRacGapTyrosine Kinase Receptorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (β1- and β2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that β1- and β2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named β3-chimaerin. Expression profile analysis revealed predominantly low levels for the β3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, β3-chimaerin has a unique N-terminal domain. Studies in cells established that β3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of β3-chimaerin for phorbol ester-induced translocation relative to β2-chimaerin suggests differential ligand accessibility to the C1 domain.Fil: Zubeldia Brenner, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Gutierrez Uzquiza, Alvaro. University of Pennsylvania; Estados UnidosFil: Barrio Real, Laura. University of Pennsylvania; Estados UnidosFil: Wang, Hongbin. University of Pennsylvania; Estados UnidosFil: Kazanietz, Marcelo Gabriel. University of Pennsylvania; Estados UnidosFil: Coluccio Leskow, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaSpringer2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32090Coluccio Leskow, Federico; Kazanietz, Marcelo Gabriel; Wang, Hongbin; Barrio Real, Laura; Gutierrez Uzquiza, Alvaro; Zubeldia Brenner, Lautaro; et al.; β3-Chimaerin, a novel member of the chimaerin Rac-GAP family; Springer; Molecular Biology Reports; 41; 4; 1-2014; 2067-20760301-48511573-4978CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11033-014-3055-3info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11033-014-3055-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:28Zoai:ri.conicet.gov.ar:11336/32090instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:29.265CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
title β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
spellingShingle β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
Zubeldia Brenner, Lautaro
Chimaerin
Rac
Gap
Tyrosine Kinase Receptor
title_short β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
title_full β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
title_fullStr β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
title_full_unstemmed β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
title_sort β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
dc.creator.none.fl_str_mv Zubeldia Brenner, Lautaro
Gutierrez Uzquiza, Alvaro
Barrio Real, Laura
Wang, Hongbin
Kazanietz, Marcelo Gabriel
Coluccio Leskow, Federico
author Zubeldia Brenner, Lautaro
author_facet Zubeldia Brenner, Lautaro
Gutierrez Uzquiza, Alvaro
Barrio Real, Laura
Wang, Hongbin
Kazanietz, Marcelo Gabriel
Coluccio Leskow, Federico
author_role author
author2 Gutierrez Uzquiza, Alvaro
Barrio Real, Laura
Wang, Hongbin
Kazanietz, Marcelo Gabriel
Coluccio Leskow, Federico
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Chimaerin
Rac
Gap
Tyrosine Kinase Receptor
topic Chimaerin
Rac
Gap
Tyrosine Kinase Receptor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (β1- and β2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that β1- and β2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named β3-chimaerin. Expression profile analysis revealed predominantly low levels for the β3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, β3-chimaerin has a unique N-terminal domain. Studies in cells established that β3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of β3-chimaerin for phorbol ester-induced translocation relative to β2-chimaerin suggests differential ligand accessibility to the C1 domain.
Fil: Zubeldia Brenner, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gutierrez Uzquiza, Alvaro. University of Pennsylvania; Estados Unidos
Fil: Barrio Real, Laura. University of Pennsylvania; Estados Unidos
Fil: Wang, Hongbin. University of Pennsylvania; Estados Unidos
Fil: Kazanietz, Marcelo Gabriel. University of Pennsylvania; Estados Unidos
Fil: Coluccio Leskow, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
description Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (β1- and β2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that β1- and β2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named β3-chimaerin. Expression profile analysis revealed predominantly low levels for the β3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, β3-chimaerin has a unique N-terminal domain. Studies in cells established that β3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of β3-chimaerin for phorbol ester-induced translocation relative to β2-chimaerin suggests differential ligand accessibility to the C1 domain.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32090
Coluccio Leskow, Federico; Kazanietz, Marcelo Gabriel; Wang, Hongbin; Barrio Real, Laura; Gutierrez Uzquiza, Alvaro; Zubeldia Brenner, Lautaro; et al.; β3-Chimaerin, a novel member of the chimaerin Rac-GAP family; Springer; Molecular Biology Reports; 41; 4; 1-2014; 2067-2076
0301-4851
1573-4978
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32090
identifier_str_mv Coluccio Leskow, Federico; Kazanietz, Marcelo Gabriel; Wang, Hongbin; Barrio Real, Laura; Gutierrez Uzquiza, Alvaro; Zubeldia Brenner, Lautaro; et al.; β3-Chimaerin, a novel member of the chimaerin Rac-GAP family; Springer; Molecular Biology Reports; 41; 4; 1-2014; 2067-2076
0301-4851
1573-4978
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11033-014-3055-3
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11033-014-3055-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269582899806208
score 13.13397

Publicaciones similares