Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)

Autores
Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Vianna, María Florencia; Abarca, Camila Lucía; Scorsetti, Ana Clara
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Entomopathogenic fungi have been used as biological control agents of insectpests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species.Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassianainoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassi-ana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference andgrowth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping.The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B.bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant sta-tistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a lowpresence. This might indicate the occurrence of antifungal activity that interferes with the establishment of theentomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepperleaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation withB. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We didnot find significant differences in the feeding preference between inoculated and control leaves, although therewas a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strainB. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effectiveinoculation technique was leaf spraying. We did not find differences of statistical significance with regard to themortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed atendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluatingthe indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to furtherexplore their potential use in the management of insect pests.
Fil: Allegrucci, Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Velázquez, María Silvana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Vianna, María Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Abarca, Camila Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Scorsetti, Ana Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
Endophytic Fungi
Inoculation techniques
Biological control agents
Aphid pest
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
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Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/139809
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network_name_str CONICET Digital (CONICET)
spelling Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)Establecimiento del hongo entomopatógeno Beauveria bassiana como endófito en Capsicum annuum y sus efectos sobre la plaga del áfido Myzus persicae (Homoptera: Aphididae)Allegrucci, NataliaVelázquez, María SilvanaRusso, Maria LeticiaVianna, María FlorenciaAbarca, Camila LucíaScorsetti, Ana ClaraEndophytic FungiInoculation techniquesBiological control agentsAphid pesthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Entomopathogenic fungi have been used as biological control agents of insectpests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species.Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassianainoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassi-ana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference andgrowth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping.The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B.bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant sta-tistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a lowpresence. This might indicate the occurrence of antifungal activity that interferes with the establishment of theentomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepperleaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation withB. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We didnot find significant differences in the feeding preference between inoculated and control leaves, although therewas a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strainB. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effectiveinoculation technique was leaf spraying. We did not find differences of statistical significance with regard to themortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed atendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluatingthe indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to furtherexplore their potential use in the management of insect pests.Fil: Allegrucci, Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Velázquez, María Silvana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Vianna, María Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Abarca, Camila Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Scorsetti, Ana Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaUniversidad de Costa Rica2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139809Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Vianna, María Florencia; Abarca, Camila Lucía; et al.; Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae); Universidad de Costa Rica; Revista de Biología Tropical; 68; 4; 12-2020; 1084-10940034-7744CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15517/RBT.V68I4.41218info:eu-repo/semantics/altIdentifier/url/https://revistas.ucr.ac.cr/index.php/rbt/article/view/41218info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:18:12Zoai:ri.conicet.gov.ar:11336/139809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:18:12.38CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
Establecimiento del hongo entomopatógeno Beauveria bassiana como endófito en Capsicum annuum y sus efectos sobre la plaga del áfido Myzus persicae (Homoptera: Aphididae)
title Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
spellingShingle Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
Allegrucci, Natalia
Endophytic Fungi
Inoculation techniques
Biological control agents
Aphid pest
title_short Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
title_full Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
title_fullStr Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
title_full_unstemmed Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
title_sort Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
dc.creator.none.fl_str_mv Allegrucci, Natalia
Velázquez, María Silvana
Russo, Maria Leticia
Vianna, María Florencia
Abarca, Camila Lucía
Scorsetti, Ana Clara
author Allegrucci, Natalia
author_facet Allegrucci, Natalia
Velázquez, María Silvana
Russo, Maria Leticia
Vianna, María Florencia
Abarca, Camila Lucía
Scorsetti, Ana Clara
author_role author
author2 Velázquez, María Silvana
Russo, Maria Leticia
Vianna, María Florencia
Abarca, Camila Lucía
Scorsetti, Ana Clara
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Endophytic Fungi
Inoculation techniques
Biological control agents
Aphid pest
topic Endophytic Fungi
Inoculation techniques
Biological control agents
Aphid pest
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: Entomopathogenic fungi have been used as biological control agents of insectpests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species.Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassianainoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassi-ana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference andgrowth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping.The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B.bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant sta-tistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a lowpresence. This might indicate the occurrence of antifungal activity that interferes with the establishment of theentomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepperleaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation withB. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We didnot find significant differences in the feeding preference between inoculated and control leaves, although therewas a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strainB. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effectiveinoculation technique was leaf spraying. We did not find differences of statistical significance with regard to themortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed atendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluatingthe indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to furtherexplore their potential use in the management of insect pests.
Fil: Allegrucci, Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Velázquez, María Silvana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Vianna, María Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Abarca, Camila Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Scorsetti, Ana Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Introduction: Entomopathogenic fungi have been used as biological control agents of insectpests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species.Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassianainoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassi-ana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference andgrowth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping.The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B.bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant sta-tistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a lowpresence. This might indicate the occurrence of antifungal activity that interferes with the establishment of theentomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepperleaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation withB. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We didnot find significant differences in the feeding preference between inoculated and control leaves, although therewas a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strainB. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effectiveinoculation technique was leaf spraying. We did not find differences of statistical significance with regard to themortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed atendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluatingthe indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to furtherexplore their potential use in the management of insect pests.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139809
Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Vianna, María Florencia; Abarca, Camila Lucía; et al.; Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae); Universidad de Costa Rica; Revista de Biología Tropical; 68; 4; 12-2020; 1084-1094
0034-7744
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139809
identifier_str_mv Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Vianna, María Florencia; Abarca, Camila Lucía; et al.; Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae); Universidad de Costa Rica; Revista de Biología Tropical; 68; 4; 12-2020; 1084-1094
0034-7744
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://revistas.ucr.ac.cr/index.php/rbt/article/view/41218
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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