Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
- Autores
- Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Vianna, María Florencia; Abarca, Camila Lucía; Scorsetti, Ana Clara
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Introduction: Entomopathogenic fungi have been used as biological control agents of insectpests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species.Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassianainoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassi-ana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference andgrowth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping.The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B.bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant sta-tistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a lowpresence. This might indicate the occurrence of antifungal activity that interferes with the establishment of theentomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepperleaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation withB. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We didnot find significant differences in the feeding preference between inoculated and control leaves, although therewas a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strainB. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effectiveinoculation technique was leaf spraying. We did not find differences of statistical significance with regard to themortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed atendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluatingthe indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to furtherexplore their potential use in the management of insect pests.
Fil: Allegrucci, Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Velázquez, María Silvana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Vianna, María Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Abarca, Camila Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Scorsetti, Ana Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina - Materia
-
Endophytic Fungi
Inoculation techniques
Biological control agents
Aphid pest - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/139809
Ver los metadatos del registro completo
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Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)Establecimiento del hongo entomopatógeno Beauveria bassiana como endófito en Capsicum annuum y sus efectos sobre la plaga del áfido Myzus persicae (Homoptera: Aphididae)Allegrucci, NataliaVelázquez, María SilvanaRusso, Maria LeticiaVianna, María FlorenciaAbarca, Camila LucíaScorsetti, Ana ClaraEndophytic FungiInoculation techniquesBiological control agentsAphid pesthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Entomopathogenic fungi have been used as biological control agents of insectpests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species.Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassianainoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassi-ana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference andgrowth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping.The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B.bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant sta-tistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a lowpresence. This might indicate the occurrence of antifungal activity that interferes with the establishment of theentomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepperleaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation withB. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We didnot find significant differences in the feeding preference between inoculated and control leaves, although therewas a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strainB. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effectiveinoculation technique was leaf spraying. We did not find differences of statistical significance with regard to themortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed atendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluatingthe indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to furtherexplore their potential use in the management of insect pests.Fil: Allegrucci, Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Velázquez, María Silvana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Vianna, María Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Abarca, Camila Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Scorsetti, Ana Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaUniversidad de Costa Rica2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139809Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Vianna, María Florencia; Abarca, Camila Lucía; et al.; Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae); Universidad de Costa Rica; Revista de Biología Tropical; 68; 4; 12-2020; 1084-10940034-7744CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15517/RBT.V68I4.41218info:eu-repo/semantics/altIdentifier/url/https://revistas.ucr.ac.cr/index.php/rbt/article/view/41218info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:57Zoai:ri.conicet.gov.ar:11336/139809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:57.424CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) Establecimiento del hongo entomopatógeno Beauveria bassiana como endófito en Capsicum annuum y sus efectos sobre la plaga del áfido Myzus persicae (Homoptera: Aphididae) |
title |
Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) |
spellingShingle |
Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) Allegrucci, Natalia Endophytic Fungi Inoculation techniques Biological control agents Aphid pest |
title_short |
Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) |
title_full |
Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) |
title_fullStr |
Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) |
title_full_unstemmed |
Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) |
title_sort |
Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) |
dc.creator.none.fl_str_mv |
Allegrucci, Natalia Velázquez, María Silvana Russo, Maria Leticia Vianna, María Florencia Abarca, Camila Lucía Scorsetti, Ana Clara |
author |
Allegrucci, Natalia |
author_facet |
Allegrucci, Natalia Velázquez, María Silvana Russo, Maria Leticia Vianna, María Florencia Abarca, Camila Lucía Scorsetti, Ana Clara |
author_role |
author |
author2 |
Velázquez, María Silvana Russo, Maria Leticia Vianna, María Florencia Abarca, Camila Lucía Scorsetti, Ana Clara |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Endophytic Fungi Inoculation techniques Biological control agents Aphid pest |
topic |
Endophytic Fungi Inoculation techniques Biological control agents Aphid pest |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Introduction: Entomopathogenic fungi have been used as biological control agents of insectpests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species.Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassianainoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassi-ana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference andgrowth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping.The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B.bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant sta-tistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a lowpresence. This might indicate the occurrence of antifungal activity that interferes with the establishment of theentomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepperleaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation withB. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We didnot find significant differences in the feeding preference between inoculated and control leaves, although therewas a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strainB. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effectiveinoculation technique was leaf spraying. We did not find differences of statistical significance with regard to themortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed atendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluatingthe indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to furtherexplore their potential use in the management of insect pests. Fil: Allegrucci, Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Velázquez, María Silvana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Vianna, María Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Abarca, Camila Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Scorsetti, Ana Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina |
description |
Introduction: Entomopathogenic fungi have been used as biological control agents of insectpests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species.Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassianainoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassi-ana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference andgrowth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping.The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B.bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant sta-tistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a lowpresence. This might indicate the occurrence of antifungal activity that interferes with the establishment of theentomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepperleaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation withB. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We didnot find significant differences in the feeding preference between inoculated and control leaves, although therewas a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strainB. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effectiveinoculation technique was leaf spraying. We did not find differences of statistical significance with regard to themortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed atendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluatingthe indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to furtherexplore their potential use in the management of insect pests. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/139809 Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Vianna, María Florencia; Abarca, Camila Lucía; et al.; Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae); Universidad de Costa Rica; Revista de Biología Tropical; 68; 4; 12-2020; 1084-1094 0034-7744 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/139809 |
identifier_str_mv |
Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Vianna, María Florencia; Abarca, Camila Lucía; et al.; Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae); Universidad de Costa Rica; Revista de Biología Tropical; 68; 4; 12-2020; 1084-1094 0034-7744 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.15517/RBT.V68I4.41218 info:eu-repo/semantics/altIdentifier/url/https://revistas.ucr.ac.cr/index.php/rbt/article/view/41218 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
publisher.none.fl_str_mv |
Universidad de Costa Rica |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614487965958144 |
score |
13.070432 |