Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance

Autores
Machado Assefh, Cristina Renata; Lòpez Isasmendi, Guadalupe; Tjallingii, W. Fred; Jander, Georg; Alvarez, Adriana Elisabet
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Myzus persicae Sulzer, like almost all aphids, associates with the endosymbiotic bacterium, Buchnera aphidicola. Although the accepted function of B. aphidicola is to complete the aphid diet with nutrients such as essential amino acids and vitamins, there is evidence that the bacteria may participate in the plant–insect interaction. Moreover, bacterial proteins with potential effector action on the metabolism of the host plant have been identified in the saliva of M. persicae. However, the possible involvement of B. aphidicola in relation to host plant acceptance by aphids needs further investigation. The aim of this study was to evaluate the effect that the disruption of the B. aphidicola–M. persicae symbiosis has on aphid feeding behaviour and on the expression of aphid salivary genes. The antibiotic rifampicin was administrated to adult aphids through artificial diets to disrupt the bacterial primary endosymbionts. Comparisons were made with control aphids, feeding from diet without rifampicin, as well as normal aphids fed on radish plants. Differences were found in the feeding behaviour of aposymbiotic aphids, which had delayed host acceptance and problems during stylet penetration into host plants. It was also found that B. aphidicola disruption down-regulated the expression of the Mp63 salivary protein gene. Together, these results indicate that B. aphidicola plays a role in plant–aphid interactions. The validity of the use of artificial diets in plant–aphid studies is also discussed.
Fil: Machado Assefh, Cristina Renata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Lòpez Isasmendi, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Tjallingii, W. Fred. EPG Systems; Países Bajos
Fil: Jander, Georg. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Alvarez, Adriana Elisabet. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Materia
Aphid Primary Endosymbiont
Aphid Salivary Genes
Artificial Diets
Epg Technique
Green Peach Aphid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50927
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptanceMachado Assefh, Cristina RenataLòpez Isasmendi, GuadalupeTjallingii, W. FredJander, GeorgAlvarez, Adriana ElisabetAphid Primary EndosymbiontAphid Salivary GenesArtificial DietsEpg TechniqueGreen Peach Aphidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Myzus persicae Sulzer, like almost all aphids, associates with the endosymbiotic bacterium, Buchnera aphidicola. Although the accepted function of B. aphidicola is to complete the aphid diet with nutrients such as essential amino acids and vitamins, there is evidence that the bacteria may participate in the plant–insect interaction. Moreover, bacterial proteins with potential effector action on the metabolism of the host plant have been identified in the saliva of M. persicae. However, the possible involvement of B. aphidicola in relation to host plant acceptance by aphids needs further investigation. The aim of this study was to evaluate the effect that the disruption of the B. aphidicola–M. persicae symbiosis has on aphid feeding behaviour and on the expression of aphid salivary genes. The antibiotic rifampicin was administrated to adult aphids through artificial diets to disrupt the bacterial primary endosymbionts. Comparisons were made with control aphids, feeding from diet without rifampicin, as well as normal aphids fed on radish plants. Differences were found in the feeding behaviour of aposymbiotic aphids, which had delayed host acceptance and problems during stylet penetration into host plants. It was also found that B. aphidicola disruption down-regulated the expression of the Mp63 salivary protein gene. Together, these results indicate that B. aphidicola plays a role in plant–aphid interactions. The validity of the use of artificial diets in plant–aphid studies is also discussed.Fil: Machado Assefh, Cristina Renata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Lòpez Isasmendi, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Tjallingii, W. Fred. EPG Systems; Países BajosFil: Jander, Georg. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Alvarez, Adriana Elisabet. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50927Machado Assefh, Cristina Renata; Lòpez Isasmendi, Guadalupe; Tjallingii, W. Fred; Jander, Georg; Alvarez, Adriana Elisabet; Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance; Springer; Arthropod-plant Interactions; 9; 5; 10-2015; 529-5411872-8855CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11829-015-9394-8info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11829-015-9394-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:16:24Zoai:ri.conicet.gov.ar:11336/50927instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:16:25.316CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
title Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
spellingShingle Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
Machado Assefh, Cristina Renata
Aphid Primary Endosymbiont
Aphid Salivary Genes
Artificial Diets
Epg Technique
Green Peach Aphid
title_short Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
title_full Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
title_fullStr Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
title_full_unstemmed Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
title_sort Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
dc.creator.none.fl_str_mv Machado Assefh, Cristina Renata
Lòpez Isasmendi, Guadalupe
Tjallingii, W. Fred
Jander, Georg
Alvarez, Adriana Elisabet
author Machado Assefh, Cristina Renata
author_facet Machado Assefh, Cristina Renata
Lòpez Isasmendi, Guadalupe
Tjallingii, W. Fred
Jander, Georg
Alvarez, Adriana Elisabet
author_role author
author2 Lòpez Isasmendi, Guadalupe
Tjallingii, W. Fred
Jander, Georg
Alvarez, Adriana Elisabet
author2_role author
author
author
author
dc.subject.none.fl_str_mv Aphid Primary Endosymbiont
Aphid Salivary Genes
Artificial Diets
Epg Technique
Green Peach Aphid
topic Aphid Primary Endosymbiont
Aphid Salivary Genes
Artificial Diets
Epg Technique
Green Peach Aphid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Myzus persicae Sulzer, like almost all aphids, associates with the endosymbiotic bacterium, Buchnera aphidicola. Although the accepted function of B. aphidicola is to complete the aphid diet with nutrients such as essential amino acids and vitamins, there is evidence that the bacteria may participate in the plant–insect interaction. Moreover, bacterial proteins with potential effector action on the metabolism of the host plant have been identified in the saliva of M. persicae. However, the possible involvement of B. aphidicola in relation to host plant acceptance by aphids needs further investigation. The aim of this study was to evaluate the effect that the disruption of the B. aphidicola–M. persicae symbiosis has on aphid feeding behaviour and on the expression of aphid salivary genes. The antibiotic rifampicin was administrated to adult aphids through artificial diets to disrupt the bacterial primary endosymbionts. Comparisons were made with control aphids, feeding from diet without rifampicin, as well as normal aphids fed on radish plants. Differences were found in the feeding behaviour of aposymbiotic aphids, which had delayed host acceptance and problems during stylet penetration into host plants. It was also found that B. aphidicola disruption down-regulated the expression of the Mp63 salivary protein gene. Together, these results indicate that B. aphidicola plays a role in plant–aphid interactions. The validity of the use of artificial diets in plant–aphid studies is also discussed.
Fil: Machado Assefh, Cristina Renata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Lòpez Isasmendi, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Tjallingii, W. Fred. EPG Systems; Países Bajos
Fil: Jander, Georg. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Alvarez, Adriana Elisabet. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
description Myzus persicae Sulzer, like almost all aphids, associates with the endosymbiotic bacterium, Buchnera aphidicola. Although the accepted function of B. aphidicola is to complete the aphid diet with nutrients such as essential amino acids and vitamins, there is evidence that the bacteria may participate in the plant–insect interaction. Moreover, bacterial proteins with potential effector action on the metabolism of the host plant have been identified in the saliva of M. persicae. However, the possible involvement of B. aphidicola in relation to host plant acceptance by aphids needs further investigation. The aim of this study was to evaluate the effect that the disruption of the B. aphidicola–M. persicae symbiosis has on aphid feeding behaviour and on the expression of aphid salivary genes. The antibiotic rifampicin was administrated to adult aphids through artificial diets to disrupt the bacterial primary endosymbionts. Comparisons were made with control aphids, feeding from diet without rifampicin, as well as normal aphids fed on radish plants. Differences were found in the feeding behaviour of aposymbiotic aphids, which had delayed host acceptance and problems during stylet penetration into host plants. It was also found that B. aphidicola disruption down-regulated the expression of the Mp63 salivary protein gene. Together, these results indicate that B. aphidicola plays a role in plant–aphid interactions. The validity of the use of artificial diets in plant–aphid studies is also discussed.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50927
Machado Assefh, Cristina Renata; Lòpez Isasmendi, Guadalupe; Tjallingii, W. Fred; Jander, Georg; Alvarez, Adriana Elisabet; Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance; Springer; Arthropod-plant Interactions; 9; 5; 10-2015; 529-541
1872-8855
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50927
identifier_str_mv Machado Assefh, Cristina Renata; Lòpez Isasmendi, Guadalupe; Tjallingii, W. Fred; Jander, Georg; Alvarez, Adriana Elisabet; Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance; Springer; Arthropod-plant Interactions; 9; 5; 10-2015; 529-541
1872-8855
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11829-015-9394-8
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11829-015-9394-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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