Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance
- Autores
- Machado Assefh, Cristina Renata; Lòpez Isasmendi, Guadalupe; Tjallingii, W. Fred; Jander, Georg; Alvarez, Adriana Elisabet
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Myzus persicae Sulzer, like almost all aphids, associates with the endosymbiotic bacterium, Buchnera aphidicola. Although the accepted function of B. aphidicola is to complete the aphid diet with nutrients such as essential amino acids and vitamins, there is evidence that the bacteria may participate in the plant–insect interaction. Moreover, bacterial proteins with potential effector action on the metabolism of the host plant have been identified in the saliva of M. persicae. However, the possible involvement of B. aphidicola in relation to host plant acceptance by aphids needs further investigation. The aim of this study was to evaluate the effect that the disruption of the B. aphidicola–M. persicae symbiosis has on aphid feeding behaviour and on the expression of aphid salivary genes. The antibiotic rifampicin was administrated to adult aphids through artificial diets to disrupt the bacterial primary endosymbionts. Comparisons were made with control aphids, feeding from diet without rifampicin, as well as normal aphids fed on radish plants. Differences were found in the feeding behaviour of aposymbiotic aphids, which had delayed host acceptance and problems during stylet penetration into host plants. It was also found that B. aphidicola disruption down-regulated the expression of the Mp63 salivary protein gene. Together, these results indicate that B. aphidicola plays a role in plant–aphid interactions. The validity of the use of artificial diets in plant–aphid studies is also discussed.
Fil: Machado Assefh, Cristina Renata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Lòpez Isasmendi, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Tjallingii, W. Fred. EPG Systems; Países Bajos
Fil: Jander, Georg. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Alvarez, Adriana Elisabet. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina - Materia
-
Aphid Primary Endosymbiont
Aphid Salivary Genes
Artificial Diets
Epg Technique
Green Peach Aphid - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/50927
Ver los metadatos del registro completo
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Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptanceMachado Assefh, Cristina RenataLòpez Isasmendi, GuadalupeTjallingii, W. FredJander, GeorgAlvarez, Adriana ElisabetAphid Primary EndosymbiontAphid Salivary GenesArtificial DietsEpg TechniqueGreen Peach Aphidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Myzus persicae Sulzer, like almost all aphids, associates with the endosymbiotic bacterium, Buchnera aphidicola. Although the accepted function of B. aphidicola is to complete the aphid diet with nutrients such as essential amino acids and vitamins, there is evidence that the bacteria may participate in the plant–insect interaction. Moreover, bacterial proteins with potential effector action on the metabolism of the host plant have been identified in the saliva of M. persicae. However, the possible involvement of B. aphidicola in relation to host plant acceptance by aphids needs further investigation. The aim of this study was to evaluate the effect that the disruption of the B. aphidicola–M. persicae symbiosis has on aphid feeding behaviour and on the expression of aphid salivary genes. The antibiotic rifampicin was administrated to adult aphids through artificial diets to disrupt the bacterial primary endosymbionts. Comparisons were made with control aphids, feeding from diet without rifampicin, as well as normal aphids fed on radish plants. Differences were found in the feeding behaviour of aposymbiotic aphids, which had delayed host acceptance and problems during stylet penetration into host plants. It was also found that B. aphidicola disruption down-regulated the expression of the Mp63 salivary protein gene. Together, these results indicate that B. aphidicola plays a role in plant–aphid interactions. The validity of the use of artificial diets in plant–aphid studies is also discussed.Fil: Machado Assefh, Cristina Renata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Lòpez Isasmendi, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Tjallingii, W. Fred. EPG Systems; Países BajosFil: Jander, Georg. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Alvarez, Adriana Elisabet. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50927Machado Assefh, Cristina Renata; Lòpez Isasmendi, Guadalupe; Tjallingii, W. Fred; Jander, Georg; Alvarez, Adriana Elisabet; Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance; Springer; Arthropod-plant Interactions; 9; 5; 10-2015; 529-5411872-8855CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11829-015-9394-8info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11829-015-9394-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:32Zoai:ri.conicet.gov.ar:11336/50927instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:33.18CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance |
title |
Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance |
spellingShingle |
Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance Machado Assefh, Cristina Renata Aphid Primary Endosymbiont Aphid Salivary Genes Artificial Diets Epg Technique Green Peach Aphid |
title_short |
Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance |
title_full |
Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance |
title_fullStr |
Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance |
title_full_unstemmed |
Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance |
title_sort |
Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance |
dc.creator.none.fl_str_mv |
Machado Assefh, Cristina Renata Lòpez Isasmendi, Guadalupe Tjallingii, W. Fred Jander, Georg Alvarez, Adriana Elisabet |
author |
Machado Assefh, Cristina Renata |
author_facet |
Machado Assefh, Cristina Renata Lòpez Isasmendi, Guadalupe Tjallingii, W. Fred Jander, Georg Alvarez, Adriana Elisabet |
author_role |
author |
author2 |
Lòpez Isasmendi, Guadalupe Tjallingii, W. Fred Jander, Georg Alvarez, Adriana Elisabet |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Aphid Primary Endosymbiont Aphid Salivary Genes Artificial Diets Epg Technique Green Peach Aphid |
topic |
Aphid Primary Endosymbiont Aphid Salivary Genes Artificial Diets Epg Technique Green Peach Aphid |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Myzus persicae Sulzer, like almost all aphids, associates with the endosymbiotic bacterium, Buchnera aphidicola. Although the accepted function of B. aphidicola is to complete the aphid diet with nutrients such as essential amino acids and vitamins, there is evidence that the bacteria may participate in the plant–insect interaction. Moreover, bacterial proteins with potential effector action on the metabolism of the host plant have been identified in the saliva of M. persicae. However, the possible involvement of B. aphidicola in relation to host plant acceptance by aphids needs further investigation. The aim of this study was to evaluate the effect that the disruption of the B. aphidicola–M. persicae symbiosis has on aphid feeding behaviour and on the expression of aphid salivary genes. The antibiotic rifampicin was administrated to adult aphids through artificial diets to disrupt the bacterial primary endosymbionts. Comparisons were made with control aphids, feeding from diet without rifampicin, as well as normal aphids fed on radish plants. Differences were found in the feeding behaviour of aposymbiotic aphids, which had delayed host acceptance and problems during stylet penetration into host plants. It was also found that B. aphidicola disruption down-regulated the expression of the Mp63 salivary protein gene. Together, these results indicate that B. aphidicola plays a role in plant–aphid interactions. The validity of the use of artificial diets in plant–aphid studies is also discussed. Fil: Machado Assefh, Cristina Renata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Lòpez Isasmendi, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Tjallingii, W. Fred. EPG Systems; Países Bajos Fil: Jander, Georg. Boyce Thompson Institute for Plant Research; Estados Unidos Fil: Alvarez, Adriana Elisabet. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina |
description |
Myzus persicae Sulzer, like almost all aphids, associates with the endosymbiotic bacterium, Buchnera aphidicola. Although the accepted function of B. aphidicola is to complete the aphid diet with nutrients such as essential amino acids and vitamins, there is evidence that the bacteria may participate in the plant–insect interaction. Moreover, bacterial proteins with potential effector action on the metabolism of the host plant have been identified in the saliva of M. persicae. However, the possible involvement of B. aphidicola in relation to host plant acceptance by aphids needs further investigation. The aim of this study was to evaluate the effect that the disruption of the B. aphidicola–M. persicae symbiosis has on aphid feeding behaviour and on the expression of aphid salivary genes. The antibiotic rifampicin was administrated to adult aphids through artificial diets to disrupt the bacterial primary endosymbionts. Comparisons were made with control aphids, feeding from diet without rifampicin, as well as normal aphids fed on radish plants. Differences were found in the feeding behaviour of aposymbiotic aphids, which had delayed host acceptance and problems during stylet penetration into host plants. It was also found that B. aphidicola disruption down-regulated the expression of the Mp63 salivary protein gene. Together, these results indicate that B. aphidicola plays a role in plant–aphid interactions. The validity of the use of artificial diets in plant–aphid studies is also discussed. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/50927 Machado Assefh, Cristina Renata; Lòpez Isasmendi, Guadalupe; Tjallingii, W. Fred; Jander, Georg; Alvarez, Adriana Elisabet; Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance; Springer; Arthropod-plant Interactions; 9; 5; 10-2015; 529-541 1872-8855 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/50927 |
identifier_str_mv |
Machado Assefh, Cristina Renata; Lòpez Isasmendi, Guadalupe; Tjallingii, W. Fred; Jander, Georg; Alvarez, Adriana Elisabet; Disrupting Buchnera aphidicola, the endosymbiotic bacteria of Myzus persicae, delays host plant acceptance; Springer; Arthropod-plant Interactions; 9; 5; 10-2015; 529-541 1872-8855 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11829-015-9394-8 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11829-015-9394-8 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |