Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs

Autores
Wolski, Erika Alejandra; Korgan, Samanta; Capezio, Silvia Beatriz; Huarte, Marcelo Atilio; Andreu, Adriana Balbina
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Potato Late Blight is the main pathogen, which affect potatoes. Due to unavailability of effective chemical control methods and the intensive use of fungicides, which leads to high environmental and production costs, the introduction of resistant cultivars represents a safe strategy to reduce the negative aspects of the disease effects. The sources of horizontal resistance to Late Blight in the Argentinean Potato Breeding Program have been varied. One of the interesting species to evaluate is Solanum tarijense since its adaptation to long days is promissory, producing tubers of good size and aspect. Many studies have demonstrated that the expression of pathogenesis related proteins (PR) differ with the level of disease resistance in potato cultivars. These results suggest that the constitutive or inducible expression of PR proteins may contribute to the resistance to P. infestans. In this study, the enzymatic activity of different PR proteins in the specie S. tarijense was measured. Two potato clones with different levels of resistance were used. These clones were more resistant to Late Blight than Bintje and Pampeana INTA cultivars, used as control. The activity of glucanases, chitinases, peroxidases and poliphenoloxidases were 8, 1.5, 2.5, and 3 times higher respectively, in S. tarijense than in Bintje and Pampeana INTA. These results together with future studies on the interaction S. tarijense-P. infestans, will allow the introgression of S. tarijense genes in breeding programs and the use of some PR proteins as biochemical markers to assist selection towards the identification of horizontal resistant cultivars.
Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Korgan, Samanta. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Capezio, Silvia Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
late blight
pathogenesis related proteins
polyphenol-oxidases
potato
potato breeding
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138382
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programsWolski, Erika AlejandraKorgan, SamantaCapezio, Silvia BeatrizHuarte, Marcelo AtilioAndreu, Adriana Balbinalate blightpathogenesis related proteinspolyphenol-oxidasespotatopotato breedinghttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Potato Late Blight is the main pathogen, which affect potatoes. Due to unavailability of effective chemical control methods and the intensive use of fungicides, which leads to high environmental and production costs, the introduction of resistant cultivars represents a safe strategy to reduce the negative aspects of the disease effects. The sources of horizontal resistance to Late Blight in the Argentinean Potato Breeding Program have been varied. One of the interesting species to evaluate is Solanum tarijense since its adaptation to long days is promissory, producing tubers of good size and aspect. Many studies have demonstrated that the expression of pathogenesis related proteins (PR) differ with the level of disease resistance in potato cultivars. These results suggest that the constitutive or inducible expression of PR proteins may contribute to the resistance to P. infestans. In this study, the enzymatic activity of different PR proteins in the specie S. tarijense was measured. Two potato clones with different levels of resistance were used. These clones were more resistant to Late Blight than Bintje and Pampeana INTA cultivars, used as control. The activity of glucanases, chitinases, peroxidases and poliphenoloxidases were 8, 1.5, 2.5, and 3 times higher respectively, in S. tarijense than in Bintje and Pampeana INTA. These results together with future studies on the interaction S. tarijense-P. infestans, will allow the introgression of S. tarijense genes in breeding programs and the use of some PR proteins as biochemical markers to assist selection towards the identification of horizontal resistant cultivars.Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Korgan, Samanta. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Capezio, Silvia Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaGlobal Science Book2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138382Wolski, Erika Alejandra; Korgan, Samanta; Capezio, Silvia Beatriz; Huarte, Marcelo Atilio; Andreu, Adriana Balbina; Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs; Global Science Book; Fruit, Vegetable and Cereal Science and Biotechnology; 3; 12-2009; 89-941752-3419CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.globalsciencebooks.info/Online/GSBOnline/OnlineFVCSB_3_SI1.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:35Zoai:ri.conicet.gov.ar:11336/138382instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:35.298CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs
title Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs
spellingShingle Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs
Wolski, Erika Alejandra
late blight
pathogenesis related proteins
polyphenol-oxidases
potato
potato breeding
title_short Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs
title_full Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs
title_fullStr Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs
title_full_unstemmed Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs
title_sort Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs
dc.creator.none.fl_str_mv Wolski, Erika Alejandra
Korgan, Samanta
Capezio, Silvia Beatriz
Huarte, Marcelo Atilio
Andreu, Adriana Balbina
author Wolski, Erika Alejandra
author_facet Wolski, Erika Alejandra
Korgan, Samanta
Capezio, Silvia Beatriz
Huarte, Marcelo Atilio
Andreu, Adriana Balbina
author_role author
author2 Korgan, Samanta
Capezio, Silvia Beatriz
Huarte, Marcelo Atilio
Andreu, Adriana Balbina
author2_role author
author
author
author
dc.subject.none.fl_str_mv late blight
pathogenesis related proteins
polyphenol-oxidases
potato
potato breeding
topic late blight
pathogenesis related proteins
polyphenol-oxidases
potato
potato breeding
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Potato Late Blight is the main pathogen, which affect potatoes. Due to unavailability of effective chemical control methods and the intensive use of fungicides, which leads to high environmental and production costs, the introduction of resistant cultivars represents a safe strategy to reduce the negative aspects of the disease effects. The sources of horizontal resistance to Late Blight in the Argentinean Potato Breeding Program have been varied. One of the interesting species to evaluate is Solanum tarijense since its adaptation to long days is promissory, producing tubers of good size and aspect. Many studies have demonstrated that the expression of pathogenesis related proteins (PR) differ with the level of disease resistance in potato cultivars. These results suggest that the constitutive or inducible expression of PR proteins may contribute to the resistance to P. infestans. In this study, the enzymatic activity of different PR proteins in the specie S. tarijense was measured. Two potato clones with different levels of resistance were used. These clones were more resistant to Late Blight than Bintje and Pampeana INTA cultivars, used as control. The activity of glucanases, chitinases, peroxidases and poliphenoloxidases were 8, 1.5, 2.5, and 3 times higher respectively, in S. tarijense than in Bintje and Pampeana INTA. These results together with future studies on the interaction S. tarijense-P. infestans, will allow the introgression of S. tarijense genes in breeding programs and the use of some PR proteins as biochemical markers to assist selection towards the identification of horizontal resistant cultivars.
Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Korgan, Samanta. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Capezio, Silvia Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Potato Late Blight is the main pathogen, which affect potatoes. Due to unavailability of effective chemical control methods and the intensive use of fungicides, which leads to high environmental and production costs, the introduction of resistant cultivars represents a safe strategy to reduce the negative aspects of the disease effects. The sources of horizontal resistance to Late Blight in the Argentinean Potato Breeding Program have been varied. One of the interesting species to evaluate is Solanum tarijense since its adaptation to long days is promissory, producing tubers of good size and aspect. Many studies have demonstrated that the expression of pathogenesis related proteins (PR) differ with the level of disease resistance in potato cultivars. These results suggest that the constitutive or inducible expression of PR proteins may contribute to the resistance to P. infestans. In this study, the enzymatic activity of different PR proteins in the specie S. tarijense was measured. Two potato clones with different levels of resistance were used. These clones were more resistant to Late Blight than Bintje and Pampeana INTA cultivars, used as control. The activity of glucanases, chitinases, peroxidases and poliphenoloxidases were 8, 1.5, 2.5, and 3 times higher respectively, in S. tarijense than in Bintje and Pampeana INTA. These results together with future studies on the interaction S. tarijense-P. infestans, will allow the introgression of S. tarijense genes in breeding programs and the use of some PR proteins as biochemical markers to assist selection towards the identification of horizontal resistant cultivars.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138382
Wolski, Erika Alejandra; Korgan, Samanta; Capezio, Silvia Beatriz; Huarte, Marcelo Atilio; Andreu, Adriana Balbina; Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs; Global Science Book; Fruit, Vegetable and Cereal Science and Biotechnology; 3; 12-2009; 89-94
1752-3419
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138382
identifier_str_mv Wolski, Erika Alejandra; Korgan, Samanta; Capezio, Silvia Beatriz; Huarte, Marcelo Atilio; Andreu, Adriana Balbina; Biochemical markers involved in the horizontal resistance to phytophthora infestans to assist potato breeding programs; Global Science Book; Fruit, Vegetable and Cereal Science and Biotechnology; 3; 12-2009; 89-94
1752-3419
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.globalsciencebooks.info/Online/GSBOnline/OnlineFVCSB_3_SI1.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Global Science Book
publisher.none.fl_str_mv Global Science Book
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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