Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus

Autores
Borges, Dyego Gonçalves Lino; de Araújo, Marcelo Augusto; Carollo, Carlos Alexandre; Hellmann Carollo, Aline Regina; Lifschitz, Adrian Luis; Conde, Mário Henrique; Green de Freitas, Mariana; dos Santos Freire, Zelina; Francielle Tutija, Juliane; Takemi Muchon Nakatani, Matheus; de Almeida Borges, Fernando
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to evaluate the in vitro effect of quercetin combined with ivermectin (IVM) on Haemonchus contortus larvae and adults with different resistance profiles and demonstrate the in vivo anthelmintic action of this combination when used in sheep naturally infected. The effect of combination was evaluated based on the analysis of the mean effective concentration (EC50) obtained for larvae using the larval migration inhibition test and for adults using the motility test on females. The tests with larvae and adults were conducted using isolates with different degrees of susceptibility to IVM (sensitive, intermediate and highly resistant). The in vivo effect was evaluated based on the reduction in the egg count (FEC) and reduction in the count of adult helminths recovered after parasitological necropsy. Using the combination of quercetin with IVM, it was observed that in larvae, quercetin did not significantly reduce the EC50 for IVM in the sensitive and highly resistant isolates, but led to a significant reduction in the EC50 for IVM in the intermediate isolate. In adults, quercetin did not significantly reduce the EC50 for IVM in any of the isolates. No significant effect of the combination was found regarding the reduction in FEC or total count of parasites. The results of the in vitro and in vivo tests performed in the present study on quercetin activity underscore the importance of evaluating resistance-reversing agents among different stages of parasite development as well as among isolates with different resistance profiles. The action of quercetin combined with IVM on the motility of H. contortus larvae and adults was influenced by the degree of resistance and development stage of the parasite. The combination was effective only on intermediate resistant larvae. No action of the combination against adults was found. Moreover, this combination, when administered through the intra-abomasal route, was not effective at reducing the FEC and parasite load of naturally infected sheep.
Fil: Borges, Dyego Gonçalves Lino. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: de Araújo, Marcelo Augusto. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Carollo, Carlos Alexandre. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Hellmann Carollo, Aline Regina. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Conde, Mário Henrique. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Green de Freitas, Mariana. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: dos Santos Freire, Zelina. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Francielle Tutija, Juliane. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Takemi Muchon Nakatani, Matheus. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: de Almeida Borges, Fernando. Universidade Federal do Mato Grosso do Sul; Brasil
Materia
ANTHELMINTIC RESISTANCE
FLAVONOIDS
MODULATION
PARASITES
RUMINANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128518
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/128518
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortusBorges, Dyego Gonçalves Linode Araújo, Marcelo AugustoCarollo, Carlos AlexandreHellmann Carollo, Aline ReginaLifschitz, Adrian LuisConde, Mário HenriqueGreen de Freitas, Marianados Santos Freire, ZelinaFrancielle Tutija, JulianeTakemi Muchon Nakatani, Matheusde Almeida Borges, FernandoANTHELMINTIC RESISTANCEFLAVONOIDSMODULATIONPARASITESRUMINANTShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of the present study was to evaluate the in vitro effect of quercetin combined with ivermectin (IVM) on Haemonchus contortus larvae and adults with different resistance profiles and demonstrate the in vivo anthelmintic action of this combination when used in sheep naturally infected. The effect of combination was evaluated based on the analysis of the mean effective concentration (EC50) obtained for larvae using the larval migration inhibition test and for adults using the motility test on females. The tests with larvae and adults were conducted using isolates with different degrees of susceptibility to IVM (sensitive, intermediate and highly resistant). The in vivo effect was evaluated based on the reduction in the egg count (FEC) and reduction in the count of adult helminths recovered after parasitological necropsy. Using the combination of quercetin with IVM, it was observed that in larvae, quercetin did not significantly reduce the EC50 for IVM in the sensitive and highly resistant isolates, but led to a significant reduction in the EC50 for IVM in the intermediate isolate. In adults, quercetin did not significantly reduce the EC50 for IVM in any of the isolates. No significant effect of the combination was found regarding the reduction in FEC or total count of parasites. The results of the in vitro and in vivo tests performed in the present study on quercetin activity underscore the importance of evaluating resistance-reversing agents among different stages of parasite development as well as among isolates with different resistance profiles. The action of quercetin combined with IVM on the motility of H. contortus larvae and adults was influenced by the degree of resistance and development stage of the parasite. The combination was effective only on intermediate resistant larvae. No action of the combination against adults was found. Moreover, this combination, when administered through the intra-abomasal route, was not effective at reducing the FEC and parasite load of naturally infected sheep.Fil: Borges, Dyego Gonçalves Lino. Universidade Federal do Mato Grosso do Sul; BrasilFil: de Araújo, Marcelo Augusto. Universidade Federal do Mato Grosso do Sul; BrasilFil: Carollo, Carlos Alexandre. Universidade Federal do Mato Grosso do Sul; BrasilFil: Hellmann Carollo, Aline Regina. Universidade Federal do Mato Grosso do Sul; BrasilFil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Conde, Mário Henrique. Universidade Federal do Mato Grosso do Sul; BrasilFil: Green de Freitas, Mariana. Universidade Federal do Mato Grosso do Sul; BrasilFil: dos Santos Freire, Zelina. Universidade Federal do Mato Grosso do Sul; BrasilFil: Francielle Tutija, Juliane. Universidade Federal do Mato Grosso do Sul; BrasilFil: Takemi Muchon Nakatani, Matheus. Universidade Federal do Mato Grosso do Sul; BrasilFil: de Almeida Borges, Fernando. Universidade Federal do Mato Grosso do Sul; BrasilElsevier Science2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128518Borges, Dyego Gonçalves Lino; de Araújo, Marcelo Augusto; Carollo, Carlos Alexandre; Hellmann Carollo, Aline Regina; Lifschitz, Adrian Luis; et al.; Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus; Elsevier Science; Acta Tropica; 201; 1-2020; 1-80001-706XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2019.105213info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0001706X19306023?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:38Zoai:ri.conicet.gov.ar:11336/128518instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:39.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
title Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
spellingShingle Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
Borges, Dyego Gonçalves Lino
ANTHELMINTIC RESISTANCE
FLAVONOIDS
MODULATION
PARASITES
RUMINANTS
title_short Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
title_full Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
title_fullStr Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
title_full_unstemmed Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
title_sort Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
dc.creator.none.fl_str_mv Borges, Dyego Gonçalves Lino
de Araújo, Marcelo Augusto
Carollo, Carlos Alexandre
Hellmann Carollo, Aline Regina
Lifschitz, Adrian Luis
Conde, Mário Henrique
Green de Freitas, Mariana
dos Santos Freire, Zelina
Francielle Tutija, Juliane
Takemi Muchon Nakatani, Matheus
de Almeida Borges, Fernando
author Borges, Dyego Gonçalves Lino
author_facet Borges, Dyego Gonçalves Lino
de Araújo, Marcelo Augusto
Carollo, Carlos Alexandre
Hellmann Carollo, Aline Regina
Lifschitz, Adrian Luis
Conde, Mário Henrique
Green de Freitas, Mariana
dos Santos Freire, Zelina
Francielle Tutija, Juliane
Takemi Muchon Nakatani, Matheus
de Almeida Borges, Fernando
author_role author
author2 de Araújo, Marcelo Augusto
Carollo, Carlos Alexandre
Hellmann Carollo, Aline Regina
Lifschitz, Adrian Luis
Conde, Mário Henrique
Green de Freitas, Mariana
dos Santos Freire, Zelina
Francielle Tutija, Juliane
Takemi Muchon Nakatani, Matheus
de Almeida Borges, Fernando
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTHELMINTIC RESISTANCE
FLAVONOIDS
MODULATION
PARASITES
RUMINANTS
topic ANTHELMINTIC RESISTANCE
FLAVONOIDS
MODULATION
PARASITES
RUMINANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of the present study was to evaluate the in vitro effect of quercetin combined with ivermectin (IVM) on Haemonchus contortus larvae and adults with different resistance profiles and demonstrate the in vivo anthelmintic action of this combination when used in sheep naturally infected. The effect of combination was evaluated based on the analysis of the mean effective concentration (EC50) obtained for larvae using the larval migration inhibition test and for adults using the motility test on females. The tests with larvae and adults were conducted using isolates with different degrees of susceptibility to IVM (sensitive, intermediate and highly resistant). The in vivo effect was evaluated based on the reduction in the egg count (FEC) and reduction in the count of adult helminths recovered after parasitological necropsy. Using the combination of quercetin with IVM, it was observed that in larvae, quercetin did not significantly reduce the EC50 for IVM in the sensitive and highly resistant isolates, but led to a significant reduction in the EC50 for IVM in the intermediate isolate. In adults, quercetin did not significantly reduce the EC50 for IVM in any of the isolates. No significant effect of the combination was found regarding the reduction in FEC or total count of parasites. The results of the in vitro and in vivo tests performed in the present study on quercetin activity underscore the importance of evaluating resistance-reversing agents among different stages of parasite development as well as among isolates with different resistance profiles. The action of quercetin combined with IVM on the motility of H. contortus larvae and adults was influenced by the degree of resistance and development stage of the parasite. The combination was effective only on intermediate resistant larvae. No action of the combination against adults was found. Moreover, this combination, when administered through the intra-abomasal route, was not effective at reducing the FEC and parasite load of naturally infected sheep.
Fil: Borges, Dyego Gonçalves Lino. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: de Araújo, Marcelo Augusto. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Carollo, Carlos Alexandre. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Hellmann Carollo, Aline Regina. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Conde, Mário Henrique. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Green de Freitas, Mariana. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: dos Santos Freire, Zelina. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Francielle Tutija, Juliane. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Takemi Muchon Nakatani, Matheus. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: de Almeida Borges, Fernando. Universidade Federal do Mato Grosso do Sul; Brasil
description The aim of the present study was to evaluate the in vitro effect of quercetin combined with ivermectin (IVM) on Haemonchus contortus larvae and adults with different resistance profiles and demonstrate the in vivo anthelmintic action of this combination when used in sheep naturally infected. The effect of combination was evaluated based on the analysis of the mean effective concentration (EC50) obtained for larvae using the larval migration inhibition test and for adults using the motility test on females. The tests with larvae and adults were conducted using isolates with different degrees of susceptibility to IVM (sensitive, intermediate and highly resistant). The in vivo effect was evaluated based on the reduction in the egg count (FEC) and reduction in the count of adult helminths recovered after parasitological necropsy. Using the combination of quercetin with IVM, it was observed that in larvae, quercetin did not significantly reduce the EC50 for IVM in the sensitive and highly resistant isolates, but led to a significant reduction in the EC50 for IVM in the intermediate isolate. In adults, quercetin did not significantly reduce the EC50 for IVM in any of the isolates. No significant effect of the combination was found regarding the reduction in FEC or total count of parasites. The results of the in vitro and in vivo tests performed in the present study on quercetin activity underscore the importance of evaluating resistance-reversing agents among different stages of parasite development as well as among isolates with different resistance profiles. The action of quercetin combined with IVM on the motility of H. contortus larvae and adults was influenced by the degree of resistance and development stage of the parasite. The combination was effective only on intermediate resistant larvae. No action of the combination against adults was found. Moreover, this combination, when administered through the intra-abomasal route, was not effective at reducing the FEC and parasite load of naturally infected sheep.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128518
Borges, Dyego Gonçalves Lino; de Araújo, Marcelo Augusto; Carollo, Carlos Alexandre; Hellmann Carollo, Aline Regina; Lifschitz, Adrian Luis; et al.; Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus; Elsevier Science; Acta Tropica; 201; 1-2020; 1-8
0001-706X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128518
identifier_str_mv Borges, Dyego Gonçalves Lino; de Araújo, Marcelo Augusto; Carollo, Carlos Alexandre; Hellmann Carollo, Aline Regina; Lifschitz, Adrian Luis; et al.; Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus; Elsevier Science; Acta Tropica; 201; 1-2020; 1-8
0001-706X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2019.105213
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0001706X19306023?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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