A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat

Autores
Podaza, Enrique Arturo; Echarte, Stella Maris; Chisari, Andrea Nancy
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pregnancy and fetal development are periods of rapid growth and cell differentiation when mother and offspring are vulnerable to changes. Adverse events during development can be linked to an increased risk for developing metabolic diseases. The aim of this work is study the effect of protein malnutrition during gestation and lactation on liver morphology and physiology. Pregnant Wistar rats of three months of age who were fed a diet containing 8% of proteins, malnourished group (M) or 20% control group (C). The male offspring of mothers M, or male offspring from mothers C were euthanized. Blood was drawn and liver were dissected. Body weights and liver were lower in the M group. Liver damage was observed by increased serum transaminase, cholesterol and triglycerides in liver. At weaning glucose in M rats was significantly lower relative to controls. Serum proteins, albumin and triglycerides, significantly decreased compared to the control. Both glycogen as proteins liver content decreased in the group M with respect to C. We observed inflammation by increasing TNF-α and IL-6. Histology showed a significant liver injury, cellular swelling was observed, hydropic degeneration, characterized by pale cytoplasm as a result of increased volume of water stored in the cells. Conclusion: The lack of protein during development, compromises the integrity of the structural and functional liver, manifested in adulthood.
Fil: Podaza, Enrique Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Echarte, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Chisari, Andrea Nancy. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Materia
Fetal Programming
Thrifty Phenotype
Low Protein Diet
Metabolic Syndrome
Liver Damage
Non-Alcoholic Fatty Liver
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85165
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/85165
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the ratPodaza, Enrique ArturoEcharte, Stella MarisChisari, Andrea NancyFetal ProgrammingThrifty PhenotypeLow Protein DietMetabolic SyndromeLiver DamageNon-Alcoholic Fatty Liverhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pregnancy and fetal development are periods of rapid growth and cell differentiation when mother and offspring are vulnerable to changes. Adverse events during development can be linked to an increased risk for developing metabolic diseases. The aim of this work is study the effect of protein malnutrition during gestation and lactation on liver morphology and physiology. Pregnant Wistar rats of three months of age who were fed a diet containing 8% of proteins, malnourished group (M) or 20% control group (C). The male offspring of mothers M, or male offspring from mothers C were euthanized. Blood was drawn and liver were dissected. Body weights and liver were lower in the M group. Liver damage was observed by increased serum transaminase, cholesterol and triglycerides in liver. At weaning glucose in M rats was significantly lower relative to controls. Serum proteins, albumin and triglycerides, significantly decreased compared to the control. Both glycogen as proteins liver content decreased in the group M with respect to C. We observed inflammation by increasing TNF-α and IL-6. Histology showed a significant liver injury, cellular swelling was observed, hydropic degeneration, characterized by pale cytoplasm as a result of increased volume of water stored in the cells. Conclusion: The lack of protein during development, compromises the integrity of the structural and functional liver, manifested in adulthood.Fil: Podaza, Enrique Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Echarte, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Chisari, Andrea Nancy. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaAustin Publishing Group2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85165Podaza, Enrique Arturo; Echarte, Stella Maris; Chisari, Andrea Nancy; A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat; Austin Publishing Group; Annals of Nutritional Disorders & Therapy; 2; 1020; 1-2015; 1-52381-8891CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.austinpublishinggroup.com/nutritional-disorders/fulltext/andt-v2-id1020.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:36Zoai:ri.conicet.gov.ar:11336/85165instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:36.721CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat
title A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat
spellingShingle A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat
Podaza, Enrique Arturo
Fetal Programming
Thrifty Phenotype
Low Protein Diet
Metabolic Syndrome
Liver Damage
Non-Alcoholic Fatty Liver
title_short A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat
title_full A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat
title_fullStr A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat
title_full_unstemmed A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat
title_sort A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat
dc.creator.none.fl_str_mv Podaza, Enrique Arturo
Echarte, Stella Maris
Chisari, Andrea Nancy
author Podaza, Enrique Arturo
author_facet Podaza, Enrique Arturo
Echarte, Stella Maris
Chisari, Andrea Nancy
author_role author
author2 Echarte, Stella Maris
Chisari, Andrea Nancy
author2_role author
author
dc.subject.none.fl_str_mv Fetal Programming
Thrifty Phenotype
Low Protein Diet
Metabolic Syndrome
Liver Damage
Non-Alcoholic Fatty Liver
topic Fetal Programming
Thrifty Phenotype
Low Protein Diet
Metabolic Syndrome
Liver Damage
Non-Alcoholic Fatty Liver
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pregnancy and fetal development are periods of rapid growth and cell differentiation when mother and offspring are vulnerable to changes. Adverse events during development can be linked to an increased risk for developing metabolic diseases. The aim of this work is study the effect of protein malnutrition during gestation and lactation on liver morphology and physiology. Pregnant Wistar rats of three months of age who were fed a diet containing 8% of proteins, malnourished group (M) or 20% control group (C). The male offspring of mothers M, or male offspring from mothers C were euthanized. Blood was drawn and liver were dissected. Body weights and liver were lower in the M group. Liver damage was observed by increased serum transaminase, cholesterol and triglycerides in liver. At weaning glucose in M rats was significantly lower relative to controls. Serum proteins, albumin and triglycerides, significantly decreased compared to the control. Both glycogen as proteins liver content decreased in the group M with respect to C. We observed inflammation by increasing TNF-α and IL-6. Histology showed a significant liver injury, cellular swelling was observed, hydropic degeneration, characterized by pale cytoplasm as a result of increased volume of water stored in the cells. Conclusion: The lack of protein during development, compromises the integrity of the structural and functional liver, manifested in adulthood.
Fil: Podaza, Enrique Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Echarte, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Chisari, Andrea Nancy. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
description Pregnancy and fetal development are periods of rapid growth and cell differentiation when mother and offspring are vulnerable to changes. Adverse events during development can be linked to an increased risk for developing metabolic diseases. The aim of this work is study the effect of protein malnutrition during gestation and lactation on liver morphology and physiology. Pregnant Wistar rats of three months of age who were fed a diet containing 8% of proteins, malnourished group (M) or 20% control group (C). The male offspring of mothers M, or male offspring from mothers C were euthanized. Blood was drawn and liver were dissected. Body weights and liver were lower in the M group. Liver damage was observed by increased serum transaminase, cholesterol and triglycerides in liver. At weaning glucose in M rats was significantly lower relative to controls. Serum proteins, albumin and triglycerides, significantly decreased compared to the control. Both glycogen as proteins liver content decreased in the group M with respect to C. We observed inflammation by increasing TNF-α and IL-6. Histology showed a significant liver injury, cellular swelling was observed, hydropic degeneration, characterized by pale cytoplasm as a result of increased volume of water stored in the cells. Conclusion: The lack of protein during development, compromises the integrity of the structural and functional liver, manifested in adulthood.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85165
Podaza, Enrique Arturo; Echarte, Stella Maris; Chisari, Andrea Nancy; A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat; Austin Publishing Group; Annals of Nutritional Disorders & Therapy; 2; 1020; 1-2015; 1-5
2381-8891
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85165
identifier_str_mv Podaza, Enrique Arturo; Echarte, Stella Maris; Chisari, Andrea Nancy; A low maternal protein diet during pregnancy and lactation induce liver offspring damage, in the rat; Austin Publishing Group; Annals of Nutritional Disorders & Therapy; 2; 1020; 1-2015; 1-5
2381-8891
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.austinpublishinggroup.com/nutritional-disorders/fulltext/andt-v2-id1020.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Austin Publishing Group
publisher.none.fl_str_mv Austin Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614169232408576
score 13.070432

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