Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways

Autores
Persia, Andres; Mariani, María Laura Lucía; Fogal, Teresa Hilda; Penissi, Alicia Beatriz
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present work was to determine whether hydroxytyrosol and oleuropein, the major polyphenols in olives and olive oil, inhibit mast cell activation induced by immune and non-immune pathways. Purified peritoneal mast cells were preincubated in the presence of test compounds (hydroxytyrosol or oleuropein), before incubation with concanavalin A, compound 48/80 or calcium ionophore A23187. Dose-response and time-dependence studies were carried out. Comparative studies with sodium chromoglycate, a classical mast cell stabilizer, were also made. The supernatants and pellets after incubations were used for determination of β-hexosaminidase content by colorimetric reaction. The percentage of β-hexosaminidase release in each tube was calculated, which was taken as a measure of mast cell activation. Other samples of cell pellets were used for cell viability studies by the trypan blue dye exclusion test, or fixed for light and electron microscopy. Biochemical and morphological findings of the present study showed for the first time that hydroxytyrosol and oleuropein inhibit the degranulation of mast cells induced by immune and non-immune pathways. These results suggest that olive polyphenols, particularly hydroxytyrosol and oleuropein, may provide insights into the development of tools useful in preventing and treating mast cell-mediated disorders.
Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Mariani, María Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Fogal, Teresa Hilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Penissi, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
Hydroxytyrosol
Oleuropein
Olive Oil
Mast Cell
Olea Europaea
Β-Hexosaminidase
Degranulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32037
Acceder
id CONICETDig_b5d453665b99b88c179758542078c27d
oai_identifier_str oai:ri.conicet.gov.ar:11336/32037
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune PathwaysPersia, AndresMariani, María Laura LucíaFogal, Teresa HildaPenissi, Alicia BeatrizHydroxytyrosolOleuropeinOlive OilMast CellOlea EuropaeaΒ-HexosaminidaseDegranulationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The aim of the present work was to determine whether hydroxytyrosol and oleuropein, the major polyphenols in olives and olive oil, inhibit mast cell activation induced by immune and non-immune pathways. Purified peritoneal mast cells were preincubated in the presence of test compounds (hydroxytyrosol or oleuropein), before incubation with concanavalin A, compound 48/80 or calcium ionophore A23187. Dose-response and time-dependence studies were carried out. Comparative studies with sodium chromoglycate, a classical mast cell stabilizer, were also made. The supernatants and pellets after incubations were used for determination of β-hexosaminidase content by colorimetric reaction. The percentage of β-hexosaminidase release in each tube was calculated, which was taken as a measure of mast cell activation. Other samples of cell pellets were used for cell viability studies by the trypan blue dye exclusion test, or fixed for light and electron microscopy. Biochemical and morphological findings of the present study showed for the first time that hydroxytyrosol and oleuropein inhibit the degranulation of mast cells induced by immune and non-immune pathways. These results suggest that olive polyphenols, particularly hydroxytyrosol and oleuropein, may provide insights into the development of tools useful in preventing and treating mast cell-mediated disorders.Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Mariani, María Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Fogal, Teresa Hilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Penissi, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaElsevier Gmbh2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32037Persia, Andres; Mariani, María Laura Lucía; Fogal, Teresa Hilda; Penissi, Alicia Beatriz; Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways; Elsevier Gmbh; Phytomedicine; 21; 11; 7-2014; 1400-14050944-7113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2014.05.010info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711314002499info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:44Zoai:ri.conicet.gov.ar:11336/32037instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:44.797CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
title Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
spellingShingle Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
Persia, Andres
Hydroxytyrosol
Oleuropein
Olive Oil
Mast Cell
Olea Europaea
Β-Hexosaminidase
Degranulation
title_short Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
title_full Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
title_fullStr Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
title_full_unstemmed Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
title_sort Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
dc.creator.none.fl_str_mv Persia, Andres
Mariani, María Laura Lucía
Fogal, Teresa Hilda
Penissi, Alicia Beatriz
author Persia, Andres
author_facet Persia, Andres
Mariani, María Laura Lucía
Fogal, Teresa Hilda
Penissi, Alicia Beatriz
author_role author
author2 Mariani, María Laura Lucía
Fogal, Teresa Hilda
Penissi, Alicia Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv Hydroxytyrosol
Oleuropein
Olive Oil
Mast Cell
Olea Europaea
Β-Hexosaminidase
Degranulation
topic Hydroxytyrosol
Oleuropein
Olive Oil
Mast Cell
Olea Europaea
Β-Hexosaminidase
Degranulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of the present work was to determine whether hydroxytyrosol and oleuropein, the major polyphenols in olives and olive oil, inhibit mast cell activation induced by immune and non-immune pathways. Purified peritoneal mast cells were preincubated in the presence of test compounds (hydroxytyrosol or oleuropein), before incubation with concanavalin A, compound 48/80 or calcium ionophore A23187. Dose-response and time-dependence studies were carried out. Comparative studies with sodium chromoglycate, a classical mast cell stabilizer, were also made. The supernatants and pellets after incubations were used for determination of β-hexosaminidase content by colorimetric reaction. The percentage of β-hexosaminidase release in each tube was calculated, which was taken as a measure of mast cell activation. Other samples of cell pellets were used for cell viability studies by the trypan blue dye exclusion test, or fixed for light and electron microscopy. Biochemical and morphological findings of the present study showed for the first time that hydroxytyrosol and oleuropein inhibit the degranulation of mast cells induced by immune and non-immune pathways. These results suggest that olive polyphenols, particularly hydroxytyrosol and oleuropein, may provide insights into the development of tools useful in preventing and treating mast cell-mediated disorders.
Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Mariani, María Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Fogal, Teresa Hilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Penissi, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description The aim of the present work was to determine whether hydroxytyrosol and oleuropein, the major polyphenols in olives and olive oil, inhibit mast cell activation induced by immune and non-immune pathways. Purified peritoneal mast cells were preincubated in the presence of test compounds (hydroxytyrosol or oleuropein), before incubation with concanavalin A, compound 48/80 or calcium ionophore A23187. Dose-response and time-dependence studies were carried out. Comparative studies with sodium chromoglycate, a classical mast cell stabilizer, were also made. The supernatants and pellets after incubations were used for determination of β-hexosaminidase content by colorimetric reaction. The percentage of β-hexosaminidase release in each tube was calculated, which was taken as a measure of mast cell activation. Other samples of cell pellets were used for cell viability studies by the trypan blue dye exclusion test, or fixed for light and electron microscopy. Biochemical and morphological findings of the present study showed for the first time that hydroxytyrosol and oleuropein inhibit the degranulation of mast cells induced by immune and non-immune pathways. These results suggest that olive polyphenols, particularly hydroxytyrosol and oleuropein, may provide insights into the development of tools useful in preventing and treating mast cell-mediated disorders.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32037
Persia, Andres; Mariani, María Laura Lucía; Fogal, Teresa Hilda; Penissi, Alicia Beatriz; Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways; Elsevier Gmbh; Phytomedicine; 21; 11; 7-2014; 1400-1405
0944-7113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32037
identifier_str_mv Persia, Andres; Mariani, María Laura Lucía; Fogal, Teresa Hilda; Penissi, Alicia Beatriz; Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways; Elsevier Gmbh; Phytomedicine; 21; 11; 7-2014; 1400-1405
0944-7113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2014.05.010
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711314002499
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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