Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis

Autores
Fernández Hurst, Nicolás; Bibolini, Mario Javier; Roth, German Alfredo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease with similar-ities to human multiple sclerosis involving peripheral activa-tion of autoreactive T cells which infiltrate the central ner-vous system and react to self antigens leading to damage. In previous studies, we have demonstrated that treatment with diazepam decreases the incidence and histological signs as-sociated with the disease and diminishes immunological re-sponses. The aim of the present work was to evaluate direct effects of diazepam on isolated T cells involved in immune responses during the development of EAE. Methods: Ani-mals were sensitized with whole myelin to induce EAE and sacrificed during the acute phase of the disease. In mono-nuclear cells isolated from popliteal lymph nodes, cell viabil-ity, apoptosis induction, proliferation and cytokine produc-tion were evaluated. Results: Diazepam did not have a toxic or proapoptotic effect on the cells, at least up to the concen-tration of 25 μ M , but proliferation, CD8+ T-cell activation and proinflammatory cytokine production were dose-depend-ently decreased. Conclusions: Diazepam has a direct inhibi-tory effect on the proliferation and activation of T lympho-cytes isolated from the main lymphoid organ involved in disease onset and this could be one of the mechanisms that contribute to the beneficial effect previously observed with diazepam in vivo during EAE development.
Fil: Fernández Hurst, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Bibolini, Mario Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Roth, German Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
Autoimmunity
Experimental Autoimmune Encephalomyelitis
Myelin Basic Protein
Benzodiazepines
T Lymphocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32103
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32103
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune EncephalomyelitisFernández Hurst, NicolásBibolini, Mario JavierRoth, German AlfredoAutoimmunityExperimental Autoimmune EncephalomyelitisMyelin Basic ProteinBenzodiazepinesT Lymphocyteshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease with similar-ities to human multiple sclerosis involving peripheral activa-tion of autoreactive T cells which infiltrate the central ner-vous system and react to self antigens leading to damage. In previous studies, we have demonstrated that treatment with diazepam decreases the incidence and histological signs as-sociated with the disease and diminishes immunological re-sponses. The aim of the present work was to evaluate direct effects of diazepam on isolated T cells involved in immune responses during the development of EAE. Methods: Ani-mals were sensitized with whole myelin to induce EAE and sacrificed during the acute phase of the disease. In mono-nuclear cells isolated from popliteal lymph nodes, cell viabil-ity, apoptosis induction, proliferation and cytokine produc-tion were evaluated. Results: Diazepam did not have a toxic or proapoptotic effect on the cells, at least up to the concen-tration of 25 μ M , but proliferation, CD8+ T-cell activation and proinflammatory cytokine production were dose-depend-ently decreased. Conclusions: Diazepam has a direct inhibi-tory effect on the proliferation and activation of T lympho-cytes isolated from the main lymphoid organ involved in disease onset and this could be one of the mechanisms that contribute to the beneficial effect previously observed with diazepam in vivo during EAE development.Fil: Fernández Hurst, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Bibolini, Mario Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Roth, German Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaKarger2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32103Fernández Hurst, Nicolás; Bibolini, Mario Javier; Roth, German Alfredo; Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis; Karger; NeuroImmunoModulation; 22; 5; 10-2014; 293-3021021-7401CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000369277info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/369277info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:48:46Zoai:ri.conicet.gov.ar:11336/32103instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:48:46.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis
title Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis
spellingShingle Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis
Fernández Hurst, Nicolás
Autoimmunity
Experimental Autoimmune Encephalomyelitis
Myelin Basic Protein
Benzodiazepines
T Lymphocytes
title_short Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis
title_full Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis
title_fullStr Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis
title_full_unstemmed Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis
title_sort Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis
dc.creator.none.fl_str_mv Fernández Hurst, Nicolás
Bibolini, Mario Javier
Roth, German Alfredo
author Fernández Hurst, Nicolás
author_facet Fernández Hurst, Nicolás
Bibolini, Mario Javier
Roth, German Alfredo
author_role author
author2 Bibolini, Mario Javier
Roth, German Alfredo
author2_role author
author
dc.subject.none.fl_str_mv Autoimmunity
Experimental Autoimmune Encephalomyelitis
Myelin Basic Protein
Benzodiazepines
T Lymphocytes
topic Autoimmunity
Experimental Autoimmune Encephalomyelitis
Myelin Basic Protein
Benzodiazepines
T Lymphocytes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease with similar-ities to human multiple sclerosis involving peripheral activa-tion of autoreactive T cells which infiltrate the central ner-vous system and react to self antigens leading to damage. In previous studies, we have demonstrated that treatment with diazepam decreases the incidence and histological signs as-sociated with the disease and diminishes immunological re-sponses. The aim of the present work was to evaluate direct effects of diazepam on isolated T cells involved in immune responses during the development of EAE. Methods: Ani-mals were sensitized with whole myelin to induce EAE and sacrificed during the acute phase of the disease. In mono-nuclear cells isolated from popliteal lymph nodes, cell viabil-ity, apoptosis induction, proliferation and cytokine produc-tion were evaluated. Results: Diazepam did not have a toxic or proapoptotic effect on the cells, at least up to the concen-tration of 25 μ M , but proliferation, CD8+ T-cell activation and proinflammatory cytokine production were dose-depend-ently decreased. Conclusions: Diazepam has a direct inhibi-tory effect on the proliferation and activation of T lympho-cytes isolated from the main lymphoid organ involved in disease onset and this could be one of the mechanisms that contribute to the beneficial effect previously observed with diazepam in vivo during EAE development.
Fil: Fernández Hurst, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Bibolini, Mario Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Roth, German Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description Objective: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease with similar-ities to human multiple sclerosis involving peripheral activa-tion of autoreactive T cells which infiltrate the central ner-vous system and react to self antigens leading to damage. In previous studies, we have demonstrated that treatment with diazepam decreases the incidence and histological signs as-sociated with the disease and diminishes immunological re-sponses. The aim of the present work was to evaluate direct effects of diazepam on isolated T cells involved in immune responses during the development of EAE. Methods: Ani-mals were sensitized with whole myelin to induce EAE and sacrificed during the acute phase of the disease. In mono-nuclear cells isolated from popliteal lymph nodes, cell viabil-ity, apoptosis induction, proliferation and cytokine produc-tion were evaluated. Results: Diazepam did not have a toxic or proapoptotic effect on the cells, at least up to the concen-tration of 25 μ M , but proliferation, CD8+ T-cell activation and proinflammatory cytokine production were dose-depend-ently decreased. Conclusions: Diazepam has a direct inhibi-tory effect on the proliferation and activation of T lympho-cytes isolated from the main lymphoid organ involved in disease onset and this could be one of the mechanisms that contribute to the beneficial effect previously observed with diazepam in vivo during EAE development.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32103
Fernández Hurst, Nicolás; Bibolini, Mario Javier; Roth, German Alfredo; Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis; Karger; NeuroImmunoModulation; 22; 5; 10-2014; 293-302
1021-7401
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32103
identifier_str_mv Fernández Hurst, Nicolás; Bibolini, Mario Javier; Roth, German Alfredo; Diazepam Inhibits Proliferation of Lymph Node Cells Isolated from Rats with Experimental Autoimmune Encephalomyelitis; Karger; NeuroImmunoModulation; 22; 5; 10-2014; 293-302
1021-7401
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000369277
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/369277
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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