Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation

Autores
Parera, Victoria; Feresin, Gabriela Egly; Parera, Carlos
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Phytoremediation is the use of plants to reduce the concentrations or toxic effects of contaminants in the environments and it´s considered an efficient, ecological and low-cost solution to the problem of heavy metal pollution. A great number of members of Fabaceae are well known to have the capacity to accumulate and tolerate heavy metal. Germination and root elongation are two critical stages in plant development that are sensitive to environmental contaminants. The aim was to determine the effect of different concentrations of Cadmium (Cd) and Nickel (Ni) on seed germination, emergence rate index (ERI) and % phytotoxicity (%P) on A. subterranea and A. pinnifolia. Seeds were treated with three levels of Cd: 500µM; 1000µM and 2000µM and Ni: 100µM; 200µM; 400µM and a control treatment (distilled water). Four replicates (25 seeds) per level of treatment and species were tested in a germination chamber. Agar media was used as germination substrate. The germinated seeds was recorded every 24h for one week. A seed was considered germinated when the emerging radicle was longer than 1 mm. The root length was measured after 10 days. Generalized linear mixed-effects models with a binomial error distribution was used for germination, and ANOVA followed by LSD Fisher for ERI and % phytoxicity. Results showed that the effect of the treatments on germination and ERI depend on the specie (treatments*specie p=0, 0001). The number of germinated seeds was significantly higher in treatments with Ni and control compared with Cd for both species. Cd 2000 µM reduced significantly the germination to 2 seeds for A. pinnifolia and 56 seeds for A.subterranea. Similar results were observed in the ERI evaluation, treatments with more than 1000µM Cd reduces significantly emergence rate compared to the control in both species. The %P of roots analyzed in all concentrations of Cd in both species was >94%, showing a high toxicity even at low concentrations. Ni showed a low %P at 100µM (41%) for A. subterranea and in the other concentrations was higher than 80%. A. pinnifolia also showed an increasing trend with increasing Ni concentration but with values above 77%. The results suggest that both Adesmia species could be considered as a potential candidate for phytoremediation. However future studies on later stages of plant growth should be tested to complete the performance.
Fil: Parera, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Feresin, Gabriela Egly. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Parera, Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan; Argentina
XXXVII Reunión Anual de La Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
HEAVY METALS
GERMINATION
PHYTOREMEDIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/161180

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network_name_str CONICET Digital (CONICET)
spelling Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediationParera, VictoriaFeresin, Gabriela EglyParera, CarlosHEAVY METALSGERMINATIONPHYTOREMEDIATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Phytoremediation is the use of plants to reduce the concentrations or toxic effects of contaminants in the environments and it´s considered an efficient, ecological and low-cost solution to the problem of heavy metal pollution. A great number of members of Fabaceae are well known to have the capacity to accumulate and tolerate heavy metal. Germination and root elongation are two critical stages in plant development that are sensitive to environmental contaminants. The aim was to determine the effect of different concentrations of Cadmium (Cd) and Nickel (Ni) on seed germination, emergence rate index (ERI) and % phytotoxicity (%P) on A. subterranea and A. pinnifolia. Seeds were treated with three levels of Cd: 500µM; 1000µM and 2000µM and Ni: 100µM; 200µM; 400µM and a control treatment (distilled water). Four replicates (25 seeds) per level of treatment and species were tested in a germination chamber. Agar media was used as germination substrate. The germinated seeds was recorded every 24h for one week. A seed was considered germinated when the emerging radicle was longer than 1 mm. The root length was measured after 10 days. Generalized linear mixed-effects models with a binomial error distribution was used for germination, and ANOVA followed by LSD Fisher for ERI and % phytoxicity. Results showed that the effect of the treatments on germination and ERI depend on the specie (treatments*specie p=0, 0001). The number of germinated seeds was significantly higher in treatments with Ni and control compared with Cd for both species. Cd 2000 µM reduced significantly the germination to 2 seeds for A. pinnifolia and 56 seeds for A.subterranea. Similar results were observed in the ERI evaluation, treatments with more than 1000µM Cd reduces significantly emergence rate compared to the control in both species. The %P of roots analyzed in all concentrations of Cd in both species was >94%, showing a high toxicity even at low concentrations. Ni showed a low %P at 100µM (41%) for A. subterranea and in the other concentrations was higher than 80%. A. pinnifolia also showed an increasing trend with increasing Ni concentration but with values above 77%. The results suggest that both Adesmia species could be considered as a potential candidate for phytoremediation. However future studies on later stages of plant growth should be tested to complete the performance.Fil: Parera, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Feresin, Gabriela Egly. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Parera, Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan; ArgentinaXXXVII Reunión Anual de La Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/161180Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation; XXXVII Reunión Anual de La Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 97-97CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/xxxv-reunion-cientifica-anual/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:58Zoai:ri.conicet.gov.ar:11336/161180instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:58.344CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation
title Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation
spellingShingle Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation
Parera, Victoria
HEAVY METALS
GERMINATION
PHYTOREMEDIATION
title_short Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation
title_full Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation
title_fullStr Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation
title_full_unstemmed Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation
title_sort Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation
dc.creator.none.fl_str_mv Parera, Victoria
Feresin, Gabriela Egly
Parera, Carlos
author Parera, Victoria
author_facet Parera, Victoria
Feresin, Gabriela Egly
Parera, Carlos
author_role author
author2 Feresin, Gabriela Egly
Parera, Carlos
author2_role author
author
dc.subject.none.fl_str_mv HEAVY METALS
GERMINATION
PHYTOREMEDIATION
topic HEAVY METALS
GERMINATION
PHYTOREMEDIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phytoremediation is the use of plants to reduce the concentrations or toxic effects of contaminants in the environments and it´s considered an efficient, ecological and low-cost solution to the problem of heavy metal pollution. A great number of members of Fabaceae are well known to have the capacity to accumulate and tolerate heavy metal. Germination and root elongation are two critical stages in plant development that are sensitive to environmental contaminants. The aim was to determine the effect of different concentrations of Cadmium (Cd) and Nickel (Ni) on seed germination, emergence rate index (ERI) and % phytotoxicity (%P) on A. subterranea and A. pinnifolia. Seeds were treated with three levels of Cd: 500µM; 1000µM and 2000µM and Ni: 100µM; 200µM; 400µM and a control treatment (distilled water). Four replicates (25 seeds) per level of treatment and species were tested in a germination chamber. Agar media was used as germination substrate. The germinated seeds was recorded every 24h for one week. A seed was considered germinated when the emerging radicle was longer than 1 mm. The root length was measured after 10 days. Generalized linear mixed-effects models with a binomial error distribution was used for germination, and ANOVA followed by LSD Fisher for ERI and % phytoxicity. Results showed that the effect of the treatments on germination and ERI depend on the specie (treatments*specie p=0, 0001). The number of germinated seeds was significantly higher in treatments with Ni and control compared with Cd for both species. Cd 2000 µM reduced significantly the germination to 2 seeds for A. pinnifolia and 56 seeds for A.subterranea. Similar results were observed in the ERI evaluation, treatments with more than 1000µM Cd reduces significantly emergence rate compared to the control in both species. The %P of roots analyzed in all concentrations of Cd in both species was >94%, showing a high toxicity even at low concentrations. Ni showed a low %P at 100µM (41%) for A. subterranea and in the other concentrations was higher than 80%. A. pinnifolia also showed an increasing trend with increasing Ni concentration but with values above 77%. The results suggest that both Adesmia species could be considered as a potential candidate for phytoremediation. However future studies on later stages of plant growth should be tested to complete the performance.
Fil: Parera, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Feresin, Gabriela Egly. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Parera, Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan; Argentina
XXXVII Reunión Anual de La Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description Phytoremediation is the use of plants to reduce the concentrations or toxic effects of contaminants in the environments and it´s considered an efficient, ecological and low-cost solution to the problem of heavy metal pollution. A great number of members of Fabaceae are well known to have the capacity to accumulate and tolerate heavy metal. Germination and root elongation are two critical stages in plant development that are sensitive to environmental contaminants. The aim was to determine the effect of different concentrations of Cadmium (Cd) and Nickel (Ni) on seed germination, emergence rate index (ERI) and % phytotoxicity (%P) on A. subterranea and A. pinnifolia. Seeds were treated with three levels of Cd: 500µM; 1000µM and 2000µM and Ni: 100µM; 200µM; 400µM and a control treatment (distilled water). Four replicates (25 seeds) per level of treatment and species were tested in a germination chamber. Agar media was used as germination substrate. The germinated seeds was recorded every 24h for one week. A seed was considered germinated when the emerging radicle was longer than 1 mm. The root length was measured after 10 days. Generalized linear mixed-effects models with a binomial error distribution was used for germination, and ANOVA followed by LSD Fisher for ERI and % phytoxicity. Results showed that the effect of the treatments on germination and ERI depend on the specie (treatments*specie p=0, 0001). The number of germinated seeds was significantly higher in treatments with Ni and control compared with Cd for both species. Cd 2000 µM reduced significantly the germination to 2 seeds for A. pinnifolia and 56 seeds for A.subterranea. Similar results were observed in the ERI evaluation, treatments with more than 1000µM Cd reduces significantly emergence rate compared to the control in both species. The %P of roots analyzed in all concentrations of Cd in both species was >94%, showing a high toxicity even at low concentrations. Ni showed a low %P at 100µM (41%) for A. subterranea and in the other concentrations was higher than 80%. A. pinnifolia also showed an increasing trend with increasing Ni concentration but with values above 77%. The results suggest that both Adesmia species could be considered as a potential candidate for phytoremediation. However future studies on later stages of plant growth should be tested to complete the performance.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/161180
Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation; XXXVII Reunión Anual de La Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 97-97
CONICET Digital
CONICET
url http://hdl.handle.net/11336/161180
identifier_str_mv Effect of heavy metals on seed germination of Adesmia subterranea and Adesmia pinnifolia: Implications for phytoremediation; XXXVII Reunión Anual de La Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 97-97
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/xxxv-reunion-cientifica-anual/
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
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