Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species

Autores
Mestre Citrinovitz, Ana Cecilia; Sestelo, Adrián Jorge; Ceballos, María Belén; Barañao, Jose Lino Salvador; Saragueta, Patricia Esther
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Buenos Aires Zoo together with scientists from IByME-CONICET have created the Biobank of Genetic Resources (BGR) dedicated to the acquisition, processing, storage, use and supply of biomaterials to the scientific and conservation industry that are viable, diverse and representative of South American wildlife populations. Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This work focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species.
Fil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sestelo, Adrián Jorge. Jardín Zoológico de la Ciudad de Buenos Aires S.A.; Argentina
Fil: Ceballos, María Belén. Jardín Zoológico de la Ciudad de Buenos Aires S.A.; Argentina
Fil: Barañao, Jose Lino Salvador. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Saragueta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Biobank
Cell Culture
Panthera Onca
Yaguarete
Jaguar
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23049
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23049
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered speciesMestre Citrinovitz, Ana CeciliaSestelo, Adrián JorgeCeballos, María BelénBarañao, Jose Lino SalvadorSaragueta, Patricia EstherBiobankCell CulturePanthera OncaYaguareteJaguarhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Buenos Aires Zoo together with scientists from IByME-CONICET have created the Biobank of Genetic Resources (BGR) dedicated to the acquisition, processing, storage, use and supply of biomaterials to the scientific and conservation industry that are viable, diverse and representative of South American wildlife populations. Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This work focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species.Fil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Sestelo, Adrián Jorge. Jardín Zoológico de la Ciudad de Buenos Aires S.A.; ArgentinaFil: Ceballos, María Belén. Jardín Zoológico de la Ciudad de Buenos Aires S.A.; ArgentinaFil: Barañao, Jose Lino Salvador. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Saragueta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaWiley2016-10-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23049Mestre Citrinovitz, Ana Cecilia; Sestelo, Adrián Jorge; Ceballos, María Belén; Barañao, Jose Lino Salvador; Saragueta, Patricia Esther; Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species; Wiley; Current Protocols in Molecular Biology; 116; 28.7; 10-10-2016; 1-141934-36391934-3647CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cpmb.25/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/cpmb.25info:eu-repo/semantics/altIdentifier/pmid/27723084info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:19Zoai:ri.conicet.gov.ar:11336/23049instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:19.654CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species
title Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species
spellingShingle Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species
Mestre Citrinovitz, Ana Cecilia
Biobank
Cell Culture
Panthera Onca
Yaguarete
Jaguar
title_short Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species
title_full Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species
title_fullStr Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species
title_full_unstemmed Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species
title_sort Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species
dc.creator.none.fl_str_mv Mestre Citrinovitz, Ana Cecilia
Sestelo, Adrián Jorge
Ceballos, María Belén
Barañao, Jose Lino Salvador
Saragueta, Patricia Esther
author Mestre Citrinovitz, Ana Cecilia
author_facet Mestre Citrinovitz, Ana Cecilia
Sestelo, Adrián Jorge
Ceballos, María Belén
Barañao, Jose Lino Salvador
Saragueta, Patricia Esther
author_role author
author2 Sestelo, Adrián Jorge
Ceballos, María Belén
Barañao, Jose Lino Salvador
Saragueta, Patricia Esther
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biobank
Cell Culture
Panthera Onca
Yaguarete
Jaguar
topic Biobank
Cell Culture
Panthera Onca
Yaguarete
Jaguar
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Buenos Aires Zoo together with scientists from IByME-CONICET have created the Biobank of Genetic Resources (BGR) dedicated to the acquisition, processing, storage, use and supply of biomaterials to the scientific and conservation industry that are viable, diverse and representative of South American wildlife populations. Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This work focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species.
Fil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sestelo, Adrián Jorge. Jardín Zoológico de la Ciudad de Buenos Aires S.A.; Argentina
Fil: Ceballos, María Belén. Jardín Zoológico de la Ciudad de Buenos Aires S.A.; Argentina
Fil: Barañao, Jose Lino Salvador. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Saragueta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description The Buenos Aires Zoo together with scientists from IByME-CONICET have created the Biobank of Genetic Resources (BGR) dedicated to the acquisition, processing, storage, use and supply of biomaterials to the scientific and conservation industry that are viable, diverse and representative of South American wildlife populations. Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This work focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23049
Mestre Citrinovitz, Ana Cecilia; Sestelo, Adrián Jorge; Ceballos, María Belén; Barañao, Jose Lino Salvador; Saragueta, Patricia Esther; Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species; Wiley; Current Protocols in Molecular Biology; 116; 28.7; 10-10-2016; 1-14
1934-3639
1934-3647
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23049
identifier_str_mv Mestre Citrinovitz, Ana Cecilia; Sestelo, Adrián Jorge; Ceballos, María Belén; Barañao, Jose Lino Salvador; Saragueta, Patricia Esther; Isolation of primary fibroblast culture from wildlife: the Panthera Onca case to preserve a South American endangered species; Wiley; Current Protocols in Molecular Biology; 116; 28.7; 10-10-2016; 1-14
1934-3639
1934-3647
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cpmb.25/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/cpmb.25
info:eu-repo/semantics/altIdentifier/pmid/27723084
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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