In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms

Autores
Delpech, Gastón; Sparo, Mónica Delfina; Bistoletti, Mariana; Lissarrague, Sabina; Cherjovsky, Mariana; Baldaccini, Maria Beatriz; García Allende, Leonardo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Background: The pathogen Staphylococcus aureus is able to persist on biotic andabiotic surfaces through biofilm formation. Enterocin AP-CECT7121 has shownhomogenous bactericidal activity in vitro against Staph. aureus of food and clinicalorigin. Objective: assess the eradication of biofilm-associated cells of multiresistant clinical Staph. aureus. Methods: 7 multi-resistant Staph. aureus(HSSA32-HSSA38) isolated from blood cultures of inpatients, from Hospital RamónSantamarina (Tandil City, Argentina) during the period January-November 2016were studied. All included patients had hip prosthesis infections. Purified APCECT7121 was used. MICs determinations were done by the micro-liquid dilutionmethod. For bactericidal activity against planktonic cells, AP-CECT7121 wasadded to staphylococcal cultures (108 CFU/mL), in a 96-well plate, to finalconcentrations of 1xMIC and 4xMIC, and incubated at 37°C. CFU counts wereperformed at 0, 4, 8, and 24 h. For assessing bactericidal activity against Staph.aureus biofilm cells, staphylococcal suspensions in Brain Heart Infusion broth-1%glucose were added to the wells and incubated for 24 h at 37°C. Formed biofilmswere incubated with the enterocin (1XMIC and 4XMIC) for 1 and 24 h. After, CFUcounts of the biofilms were done. Results: bactericidal activity of AP-CECT7121against planktonic cell was detected between 1- 4 h of incubation. A dosedependent effect of AP-CECT7121 against staphylococcal biofilms was observed.AP-CECT7121 (4XMIC) showed bactericidal activity against biofilm cells, butcomplete killing was not achieved. Conclusion: AP-CECT7121 showed a significantefficacy against staphylococcal biofilms and constitutes a candidate for futureapplications in the prevention and treatment of biofilm-associated infections
Fil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bistoletti, Mariana. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina
Fil: Lissarrague, Sabina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina
Fil: Cherjovsky, Mariana. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina
Fil: Baldaccini, Maria Beatriz. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina
Fil: García Allende, Leonardo. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina
7th Congress of European Microbiologists
España
Federation of European Microbiological Societies
Materia
AP-CECT7121
STAPHYLOCOCCUS AUREUS
MULTIRESISTANT
BIOFILMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/183882
Acceder
id CONICETDig_b3f057438efdff992ec32b3d67dc6e88
oai_identifier_str oai:ri.conicet.gov.ar:11336/183882
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilmsDelpech, GastónSparo, Mónica DelfinaBistoletti, MarianaLissarrague, SabinaCherjovsky, MarianaBaldaccini, Maria BeatrizGarcía Allende, LeonardoAP-CECT7121STAPHYLOCOCCUS AUREUSMULTIRESISTANTBIOFILMShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: The pathogen Staphylococcus aureus is able to persist on biotic andabiotic surfaces through biofilm formation. Enterocin AP-CECT7121 has shownhomogenous bactericidal activity in vitro against Staph. aureus of food and clinicalorigin. Objective: assess the eradication of biofilm-associated cells of multiresistant clinical Staph. aureus. Methods: 7 multi-resistant Staph. aureus(HSSA32-HSSA38) isolated from blood cultures of inpatients, from Hospital RamónSantamarina (Tandil City, Argentina) during the period January-November 2016were studied. All included patients had hip prosthesis infections. Purified APCECT7121 was used. MICs determinations were done by the micro-liquid dilutionmethod. For bactericidal activity against planktonic cells, AP-CECT7121 wasadded to staphylococcal cultures (108 CFU/mL), in a 96-well plate, to finalconcentrations of 1xMIC and 4xMIC, and incubated at 37°C. CFU counts wereperformed at 0, 4, 8, and 24 h. For assessing bactericidal activity against Staph.aureus biofilm cells, staphylococcal suspensions in Brain Heart Infusion broth-1%glucose were added to the wells and incubated for 24 h at 37°C. Formed biofilmswere incubated with the enterocin (1XMIC and 4XMIC) for 1 and 24 h. After, CFUcounts of the biofilms were done. Results: bactericidal activity of AP-CECT7121against planktonic cell was detected between 1- 4 h of incubation. A dosedependent effect of AP-CECT7121 against staphylococcal biofilms was observed.AP-CECT7121 (4XMIC) showed bactericidal activity against biofilm cells, butcomplete killing was not achieved. Conclusion: AP-CECT7121 showed a significantefficacy against staphylococcal biofilms and constitutes a candidate for futureapplications in the prevention and treatment of biofilm-associated infectionsFil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Bistoletti, Mariana. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; ArgentinaFil: Lissarrague, Sabina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; ArgentinaFil: Cherjovsky, Mariana. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; ArgentinaFil: Baldaccini, Maria Beatriz. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; ArgentinaFil: García Allende, Leonardo. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina7th Congress of European MicrobiologistsEspañaFederation of European Microbiological SocietiesFederation of European Microbiological Societies2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/183882In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms; 7th Congress of European Microbiologists; España; 2017; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://fems-microbiology.org/app/uploads/2020/05/FEMS2017_abstracts-book.pdfinfo:eu-repo/semantics/altIdentifier/url/https://fems-microbiology2017.kenes.com/index.htmlInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:07Zoai:ri.conicet.gov.ar:11336/183882instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:07.901CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms
title In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms
spellingShingle In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms
Delpech, Gastón
AP-CECT7121
STAPHYLOCOCCUS AUREUS
MULTIRESISTANT
BIOFILMS
title_short In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms
title_full In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms
title_fullStr In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms
title_full_unstemmed In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms
title_sort In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms
dc.creator.none.fl_str_mv Delpech, Gastón
Sparo, Mónica Delfina
Bistoletti, Mariana
Lissarrague, Sabina
Cherjovsky, Mariana
Baldaccini, Maria Beatriz
García Allende, Leonardo
author Delpech, Gastón
author_facet Delpech, Gastón
Sparo, Mónica Delfina
Bistoletti, Mariana
Lissarrague, Sabina
Cherjovsky, Mariana
Baldaccini, Maria Beatriz
García Allende, Leonardo
author_role author
author2 Sparo, Mónica Delfina
Bistoletti, Mariana
Lissarrague, Sabina
Cherjovsky, Mariana
Baldaccini, Maria Beatriz
García Allende, Leonardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AP-CECT7121
STAPHYLOCOCCUS AUREUS
MULTIRESISTANT
BIOFILMS
topic AP-CECT7121
STAPHYLOCOCCUS AUREUS
MULTIRESISTANT
BIOFILMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: The pathogen Staphylococcus aureus is able to persist on biotic andabiotic surfaces through biofilm formation. Enterocin AP-CECT7121 has shownhomogenous bactericidal activity in vitro against Staph. aureus of food and clinicalorigin. Objective: assess the eradication of biofilm-associated cells of multiresistant clinical Staph. aureus. Methods: 7 multi-resistant Staph. aureus(HSSA32-HSSA38) isolated from blood cultures of inpatients, from Hospital RamónSantamarina (Tandil City, Argentina) during the period January-November 2016were studied. All included patients had hip prosthesis infections. Purified APCECT7121 was used. MICs determinations were done by the micro-liquid dilutionmethod. For bactericidal activity against planktonic cells, AP-CECT7121 wasadded to staphylococcal cultures (108 CFU/mL), in a 96-well plate, to finalconcentrations of 1xMIC and 4xMIC, and incubated at 37°C. CFU counts wereperformed at 0, 4, 8, and 24 h. For assessing bactericidal activity against Staph.aureus biofilm cells, staphylococcal suspensions in Brain Heart Infusion broth-1%glucose were added to the wells and incubated for 24 h at 37°C. Formed biofilmswere incubated with the enterocin (1XMIC and 4XMIC) for 1 and 24 h. After, CFUcounts of the biofilms were done. Results: bactericidal activity of AP-CECT7121against planktonic cell was detected between 1- 4 h of incubation. A dosedependent effect of AP-CECT7121 against staphylococcal biofilms was observed.AP-CECT7121 (4XMIC) showed bactericidal activity against biofilm cells, butcomplete killing was not achieved. Conclusion: AP-CECT7121 showed a significantefficacy against staphylococcal biofilms and constitutes a candidate for futureapplications in the prevention and treatment of biofilm-associated infections
Fil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bistoletti, Mariana. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina
Fil: Lissarrague, Sabina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina
Fil: Cherjovsky, Mariana. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina
Fil: Baldaccini, Maria Beatriz. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina
Fil: García Allende, Leonardo. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina
7th Congress of European Microbiologists
España
Federation of European Microbiological Societies
description Background: The pathogen Staphylococcus aureus is able to persist on biotic andabiotic surfaces through biofilm formation. Enterocin AP-CECT7121 has shownhomogenous bactericidal activity in vitro against Staph. aureus of food and clinicalorigin. Objective: assess the eradication of biofilm-associated cells of multiresistant clinical Staph. aureus. Methods: 7 multi-resistant Staph. aureus(HSSA32-HSSA38) isolated from blood cultures of inpatients, from Hospital RamónSantamarina (Tandil City, Argentina) during the period January-November 2016were studied. All included patients had hip prosthesis infections. Purified APCECT7121 was used. MICs determinations were done by the micro-liquid dilutionmethod. For bactericidal activity against planktonic cells, AP-CECT7121 wasadded to staphylococcal cultures (108 CFU/mL), in a 96-well plate, to finalconcentrations of 1xMIC and 4xMIC, and incubated at 37°C. CFU counts wereperformed at 0, 4, 8, and 24 h. For assessing bactericidal activity against Staph.aureus biofilm cells, staphylococcal suspensions in Brain Heart Infusion broth-1%glucose were added to the wells and incubated for 24 h at 37°C. Formed biofilmswere incubated with the enterocin (1XMIC and 4XMIC) for 1 and 24 h. After, CFUcounts of the biofilms were done. Results: bactericidal activity of AP-CECT7121against planktonic cell was detected between 1- 4 h of incubation. A dosedependent effect of AP-CECT7121 against staphylococcal biofilms was observed.AP-CECT7121 (4XMIC) showed bactericidal activity against biofilm cells, butcomplete killing was not achieved. Conclusion: AP-CECT7121 showed a significantefficacy against staphylococcal biofilms and constitutes a candidate for futureapplications in the prevention and treatment of biofilm-associated infections
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/183882
In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms; 7th Congress of European Microbiologists; España; 2017; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/183882
identifier_str_mv In vitro activity of enterocin AP-CECT7121 against multi-resistant Staphylococcus aureus biofilms; 7th Congress of European Microbiologists; España; 2017; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://fems-microbiology.org/app/uploads/2020/05/FEMS2017_abstracts-book.pdf
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Federation of European Microbiological Societies
publisher.none.fl_str_mv Federation of European Microbiological Societies
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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