Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection

Autores
Eriko, Chiba; Tomosada, Yohsuke; Vizoso Pinto, María Guadalupe; Salva, Maria Susana; Takahashi, Takuya; Tsukida, Kohichiro; Kitazawa, Haruki; Alvarez, Gladis Susana; Villena, Julio Cesar
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previously we showed that orally administered Lactobacillus rhamnosus CRL1505 beneficially regulated the balance between pro- and anti-inflammatory mediators in the lungs of poly(I:C)-challenged mice, allowing an effective inflammatory response against the TLR3/RIG-I agonist but at the same time reducing tissue damage. The aim of the present study was to investigate whether oral administration of the CRL1505 strain was able to improve resistance against respiratory syncytial virus (RSV) infection in infant mice and to evaluate the immunological mechanisms involved in the immunobiotic effect. We demonstrated that treatment of 3-week old BALB/c mice with L. rhamnosus CRL1505 significantly reduce lung viral loads and tissue injuries after the challenge with RSV. Moreover, we showed that the protective effect achieved by the CRL1505 strain is related to its capacity to differentially modulate respiratory antiviral immune response. Our results shows that IFN-γ and IL-10 secreted in response to L. rhamnosus CRL1505 oral stimulation would modulate the pulmonary innate immune microenvironment conducting to the activation of CD103+ and CD11bhigh dendritic cells and the generation of CD3+CD4+IFN-γ+ Th1 cells with the consequent attenuation of the strong and damaging Th2 reactions associated with RSV challenge. Our results indicate that modulation of the common mucosal immune system by immunobiotics could favor protective immunity against respiratory viral pathogens with a high attack rate in early infancy, such as RSV.
Fil: Eriko, Chiba. Tohoku University; Japón
Fil: Tomosada, Yohsuke. Tohoku University; Japón
Fil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucuman. Facultad de Medicina. Departamento Biomedico. Laboratorio de Fisiologia y Farmacologia Vascular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Takahashi, Takuya. Tohoku University; Japón
Fil: Tsukida, Kohichiro. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Clínica Aplicada; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Materia
ANTIVIRAL IMMUNITY
CRL1505
L. RHAMNOSUS
RESPIRATORY SYNCYTIAL VIRUS
RESPIRATORY TRACT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2366
Acceder
id CONICETDig_b3d7d3571c8c1915418be91a3541d563
oai_identifier_str oai:ri.conicet.gov.ar:11336/2366
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infectionEriko, ChibaTomosada, YohsukeVizoso Pinto, María GuadalupeSalva, Maria SusanaTakahashi, TakuyaTsukida, KohichiroKitazawa, HarukiAlvarez, Gladis SusanaVillena, Julio CesarANTIVIRAL IMMUNITYCRL1505L. RHAMNOSUSRESPIRATORY SYNCYTIAL VIRUSRESPIRATORY TRACThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previously we showed that orally administered Lactobacillus rhamnosus CRL1505 beneficially regulated the balance between pro- and anti-inflammatory mediators in the lungs of poly(I:C)-challenged mice, allowing an effective inflammatory response against the TLR3/RIG-I agonist but at the same time reducing tissue damage. The aim of the present study was to investigate whether oral administration of the CRL1505 strain was able to improve resistance against respiratory syncytial virus (RSV) infection in infant mice and to evaluate the immunological mechanisms involved in the immunobiotic effect. We demonstrated that treatment of 3-week old BALB/c mice with L. rhamnosus CRL1505 significantly reduce lung viral loads and tissue injuries after the challenge with RSV. Moreover, we showed that the protective effect achieved by the CRL1505 strain is related to its capacity to differentially modulate respiratory antiviral immune response. Our results shows that IFN-γ and IL-10 secreted in response to L. rhamnosus CRL1505 oral stimulation would modulate the pulmonary innate immune microenvironment conducting to the activation of CD103+ and CD11bhigh dendritic cells and the generation of CD3+CD4+IFN-γ+ Th1 cells with the consequent attenuation of the strong and damaging Th2 reactions associated with RSV challenge. Our results indicate that modulation of the common mucosal immune system by immunobiotics could favor protective immunity against respiratory viral pathogens with a high attack rate in early infancy, such as RSV.Fil: Eriko, Chiba. Tohoku University; JapónFil: Tomosada, Yohsuke. Tohoku University; JapónFil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucuman. Facultad de Medicina. Departamento Biomedico. Laboratorio de Fisiologia y Farmacologia Vascular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Takahashi, Takuya. Tohoku University; JapónFil: Tsukida, Kohichiro. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Clínica Aplicada; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaElsevier Science2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2366Eriko, Chiba; Tomosada, Yohsuke; Vizoso Pinto, María Guadalupe; Salva, Maria Susana; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection; Elsevier Science; International Immunopharmacology; 17; 2; 10-2013; 373-3821567-5769enginfo:eu-repo/semantics/altIdentifier/url/http://sciencedirect.com/science/journal/15675769info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567576913002658info:eu-repo/semantics/altIdentifier/doi/10.1016/j.intimp.2013.06.024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:36Zoai:ri.conicet.gov.ar:11336/2366instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:36.646CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection
title Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection
spellingShingle Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection
Eriko, Chiba
ANTIVIRAL IMMUNITY
CRL1505
L. RHAMNOSUS
RESPIRATORY SYNCYTIAL VIRUS
RESPIRATORY TRACT
title_short Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection
title_full Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection
title_fullStr Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection
title_full_unstemmed Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection
title_sort Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection
dc.creator.none.fl_str_mv Eriko, Chiba
Tomosada, Yohsuke
Vizoso Pinto, María Guadalupe
Salva, Maria Susana
Takahashi, Takuya
Tsukida, Kohichiro
Kitazawa, Haruki
Alvarez, Gladis Susana
Villena, Julio Cesar
author Eriko, Chiba
author_facet Eriko, Chiba
Tomosada, Yohsuke
Vizoso Pinto, María Guadalupe
Salva, Maria Susana
Takahashi, Takuya
Tsukida, Kohichiro
Kitazawa, Haruki
Alvarez, Gladis Susana
Villena, Julio Cesar
author_role author
author2 Tomosada, Yohsuke
Vizoso Pinto, María Guadalupe
Salva, Maria Susana
Takahashi, Takuya
Tsukida, Kohichiro
Kitazawa, Haruki
Alvarez, Gladis Susana
Villena, Julio Cesar
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIVIRAL IMMUNITY
CRL1505
L. RHAMNOSUS
RESPIRATORY SYNCYTIAL VIRUS
RESPIRATORY TRACT
topic ANTIVIRAL IMMUNITY
CRL1505
L. RHAMNOSUS
RESPIRATORY SYNCYTIAL VIRUS
RESPIRATORY TRACT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previously we showed that orally administered Lactobacillus rhamnosus CRL1505 beneficially regulated the balance between pro- and anti-inflammatory mediators in the lungs of poly(I:C)-challenged mice, allowing an effective inflammatory response against the TLR3/RIG-I agonist but at the same time reducing tissue damage. The aim of the present study was to investigate whether oral administration of the CRL1505 strain was able to improve resistance against respiratory syncytial virus (RSV) infection in infant mice and to evaluate the immunological mechanisms involved in the immunobiotic effect. We demonstrated that treatment of 3-week old BALB/c mice with L. rhamnosus CRL1505 significantly reduce lung viral loads and tissue injuries after the challenge with RSV. Moreover, we showed that the protective effect achieved by the CRL1505 strain is related to its capacity to differentially modulate respiratory antiviral immune response. Our results shows that IFN-γ and IL-10 secreted in response to L. rhamnosus CRL1505 oral stimulation would modulate the pulmonary innate immune microenvironment conducting to the activation of CD103+ and CD11bhigh dendritic cells and the generation of CD3+CD4+IFN-γ+ Th1 cells with the consequent attenuation of the strong and damaging Th2 reactions associated with RSV challenge. Our results indicate that modulation of the common mucosal immune system by immunobiotics could favor protective immunity against respiratory viral pathogens with a high attack rate in early infancy, such as RSV.
Fil: Eriko, Chiba. Tohoku University; Japón
Fil: Tomosada, Yohsuke. Tohoku University; Japón
Fil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucuman. Facultad de Medicina. Departamento Biomedico. Laboratorio de Fisiologia y Farmacologia Vascular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Takahashi, Takuya. Tohoku University; Japón
Fil: Tsukida, Kohichiro. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Clínica Aplicada; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
description Previously we showed that orally administered Lactobacillus rhamnosus CRL1505 beneficially regulated the balance between pro- and anti-inflammatory mediators in the lungs of poly(I:C)-challenged mice, allowing an effective inflammatory response against the TLR3/RIG-I agonist but at the same time reducing tissue damage. The aim of the present study was to investigate whether oral administration of the CRL1505 strain was able to improve resistance against respiratory syncytial virus (RSV) infection in infant mice and to evaluate the immunological mechanisms involved in the immunobiotic effect. We demonstrated that treatment of 3-week old BALB/c mice with L. rhamnosus CRL1505 significantly reduce lung viral loads and tissue injuries after the challenge with RSV. Moreover, we showed that the protective effect achieved by the CRL1505 strain is related to its capacity to differentially modulate respiratory antiviral immune response. Our results shows that IFN-γ and IL-10 secreted in response to L. rhamnosus CRL1505 oral stimulation would modulate the pulmonary innate immune microenvironment conducting to the activation of CD103+ and CD11bhigh dendritic cells and the generation of CD3+CD4+IFN-γ+ Th1 cells with the consequent attenuation of the strong and damaging Th2 reactions associated with RSV challenge. Our results indicate that modulation of the common mucosal immune system by immunobiotics could favor protective immunity against respiratory viral pathogens with a high attack rate in early infancy, such as RSV.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2366
Eriko, Chiba; Tomosada, Yohsuke; Vizoso Pinto, María Guadalupe; Salva, Maria Susana; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection; Elsevier Science; International Immunopharmacology; 17; 2; 10-2013; 373-382
1567-5769
url http://hdl.handle.net/11336/2366
identifier_str_mv Eriko, Chiba; Tomosada, Yohsuke; Vizoso Pinto, María Guadalupe; Salva, Maria Susana; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection; Elsevier Science; International Immunopharmacology; 17; 2; 10-2013; 373-382
1567-5769
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://sciencedirect.com/science/journal/15675769
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567576913002658
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.intimp.2013.06.024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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